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Uses and Gratification on eWOM Tourism Information
Yang,Sung-Soo,Huh,Hyang-Jin,Suh,Yong-Kun 세계문화관광학회 2009 Conference Proceedings Vol.10 No.0
This study draw on the uses and gratifications perspective in tourism information research to examine the tourist experience associated with website. The goal of this study is to investigate the extent to eWOM(electronic Word-of-Mouth) using motivation and gratification. In methodological sense, the survey was employed in user of destination search for travel and performed application with AVOVA for analysis. Data collecting method was a convenience sampling with face-to-face interview. A total of 600 questionnaires were collected from the survey and 577 questionnaires were coded for a data for analysis on this study. Data for the study were drawn from the user who have been travel information on eWOM. The findings deriving from this study are followings; First, Perception of using motivation is consist of 5 dimensions such as Self-Development, Information Acquisition, Enjoyment, Community, and Travel Cost. Second, The findings deriving from this study is a statistically significant difference in the relationship between the using dimensions and user propensity of eWOM tourism information. Finally, using motivation was positively related with behavioral intentions of making process.
( Sul Lee ),( Hyung Joon Yoon ),( Eun Hye Jeon ),( Juseok Yang ),( Yong Jung Song ),( Hyun Jin Roh ),( Sang Hun Lee ),( Byung Su Kwon ),( Ki Hyung Kim ),( Dong Soo Suh ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-
Objective: The aim of this study is to evaluate oncologic outcomes of total abdominal radical hysterectomy (TARH), total laparoscopic radical hysterectomy (TLRH) and laparoscopic-assisted radical vaginal hysterectomy (LARVH) in early cervical cancer patients (stage IB2-3, tumor size≥2cm), analyzing the risk factors that may affect the clinical results. Methods: A total of 756 patients underwent radical hysterectomy between 2000 and 2019 from three university hospitals (Pusan National University Hospital, Yang-san Pusan National University Hospital, Ulsan University Hospital) were enrolled. Among them, 172 patients with stage IB2-3 were analyzed retrospectively. Results: Seventy-seven patients were treated with TARH, and 95 were managed via minimally invasive surgery (MIS), consisting of 35 with TLRH and 60 with LARVH. TARH group had better disease five-year disease free survival (DFS) and overall survival (OS) compared with MIS group (86.4% vs. 73.4 for DFS and 91.7 vs. 82.2 for OS), but not statistically significant in both (p=0.058 for DFS and p=0.119 for OS). When TARH and the LARVH group were compared, both five-year DFS and OS were not significant different (86.4 vs 82.2%; p=0.288 for DFS and 91.7 vs 84.4%; p=0.206 for OS). However, when TARH and the TLRH group were compared, TLRH group had significantly worse five-year DFS than TARH (86.4 vs 54.5%; p=0.008), but five-year OS was not significant different (91.7 vs 77.5%; p=0.127). Multivariate analysis showed that LN metastasis and TLRH surgical approaches were an independent prognostic factor for DFS in IB2-3 cervical cancers. Conclusion: Our findings provided the evidence that TLRH surgical approach was associated with worse DFS in IB2-3 cervical cancers but that of LARVH was not. Such results may have important implications of colpotomy method for MIS in cervical cancer.
Ginsenoside Composition Changes in Ginseng Extracts by Different Ascorbic Acid Treatments
Sung Kwon Ko,Ok Sun Cho,Hye Min Bae,Uy Dong Sohn,Byung Ok Im,Soon Hyun Cho,Byung Wook Yang,Sung Hyun Chung,Wang Soo Shin,Boo Yong Lee 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.4
The purpose of this study was to develop a new preparation process for chemical transformation of ginseng saponin glycosides to prosapogenins. Ginseng and ginseng extracts were processed under several treatment conditions using ascorbic acid solution. Treating with ascorbic acid at pH 2-3 and above 80℃ increased the ginsenoside Rg<sub>3</sub> content of samples to over 3% as compared to other pH levels and temperatures. In addition, ginseng and ginseng extracts that were processed under a high ascorbic acid solution treatment condition (pH 2.0, 5 hr) contained more ginsenoside Rg3 (approximately 16 times) than those processed under a low ascorbic acid solution treatment condition (pH 3.0, 5 hr). The highest quantity of ginsenoside Rg<sub>3</sub> (3.434%) occurred when a sample of fine ginseng root extract (AG2-9) was processed with the ascorbic acid solution at pH 2.0 for 9 hr. However, there was no change in the amount of ginsenoside Rg<sub>3</sub> when fine ginseng root extracts were processed with ascorbic acid solution at pH 2.0 for over 9 hr. In conclusion, the results indicated that ascorbic acid treatment of ginseng extracts can produce a level of ginsenoside Rg<sub>3<sub> that is over 90-fold the amount found in commercial red ginseng.
Changes in Ginsenoside Composition of White Ginseng by Fermentation
Sung Kwon Ko,Ok Sun Cho,Hye Min Bae,Byung Wook Yang,Byung-Ok Im,Young Tae Hahm,Kyung Nam Kim,Soon Hyun Cho,Jae Young Kim,Sung Hyun Chung,Boo Yong Lee 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.1
The purpose of the study was to develop a new process to manufacture ginseng extract containing saponin aglycon of high concentration. The process to transform saponin glycosides to saponin aglycon was analyzed by high performance liquid chromatography (HPLC). GCK-1 (open cultured mixture for 1 day at 42℃) had the highest content of protopanaxadiol (0.662%). However, other mixtures (GCK-2, 3, 4, 5, and 6) had less than 0.152% in the content of protopanaxadiol. In case of fermentation by inoculation of Bacillus natto, BNG-5 (B. natto inoculated mixture for 5 days at 42℃) showed the highest content of protopanaxadiol (0.364%). Other mixtures (BNG-1, 2, 3, 4, and 6) also showed the high content of more than 0.2% in protopanaxadiol. B. natto inoculation or open culture fermentation with soybean transformed ginseng saponin glycosides into saponin aglycon.
Yang, Dongsoo,Yoo, Seung Min,Gu, Changdai,Ryu, Jae Yong,Lee, Jae Eun,Lee, Sang Yup Elsevier 2019 Metabolic engineering Vol.54 No.-
<P><B>Abstract</B></P> <P>Synthetic small regulatory RNA (sRNA) can efficiently downregulate target gene expression at translational level in metabolic engineering, but cannot be used in engineered strain already having incompatible plasmid(s). To address this problem and make the sRNA gene expression modulation platform universally applicable, we report the development and applications of expanded synthetic sRNA expression platforms for rapid, multiplexed and genome-scale target gene knockdown in engineered <I>Escherichia coli</I>. As proof-of-concept, high performance strains capable of producing L-proline (54.1 g l<SUP>−1</SUP>) and L-threonine (22.9 g l<SUP>−1</SUP>) are rapidly developed by combinatorial knockdown of up to three genes via one-step co-transformation of sRNA expression vectors. Furthermore, a genome-scale sRNA library targeting 1,858 <I>E. coli</I> genes is employed to construct crude violacein (5.19 g l<SUP>−1</SUP>) and indigo (135 mg l<SUP>−1</SUP>) producers by high-throughput colorimetric screening. These examples demonstrate that the expanded sRNA expression vectors developed here enables rapid development of chemical overproducers regardless of plasmid compatibility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Various origins of replication and antibiotic markers in <I>E. coli</I> were analyzed. </LI> <LI> Expanded sRNA expression platforms were constructed to allow universal application. </LI> <LI> Combinatorial target gene knockdowns enhanced proline and threonine production. </LI> <LI> A method to rapidly construct a genome-scale sRNA library was developed. </LI> <LI> Colorimetric screening was used to develop violacein and indigo overproducers. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Yang, Do-Hyeon,Lee, Chang-Soo,Jeon, Beung-Hoon,Choi, Sung Mook,Kim, Yong-Dae,Shin, Jae Sup,Kim, Heon Elsevier 2017 Journal of industrial and engineering chemistry Vol.51 No.-
<P><B>Abstract</B></P> <P>A novel electrochemical nanofilm sensor for 1-hydroxypyrene (1-OHP) was developed based on a molecular imprinted TiO<SUB>2</SUB> gel matrix, which was prepared with Ti(O-<I>n</I>-Bu)<SUB>4</SUB> and 1-OHP using sol–gel technology. Cyclic voltammetry (CV) showed that guest binding on the non-imprinted sensor resulted in nearly the same current changes for all guest molecules, indicating non-specific binding onto the TiO<SUB>2</SUB> gel matrix. In contrast, binding at the molecular imprinted sensor revealed that the selectivity for 1-OHP relative to structurally related guest molecules was estimated to be 1.8–6.7 times greater at 1×10<SUP>−9</SUP> M, reflecting its specific adsorption and binding capacity for the template molecule.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>