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SYNTHESIS OF CaWO 4 AND CaWO 4 :Eu MICROSPHERES BY PRECIPITATION
YUGUO YANG,XUPING WANG,BING LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.1
Nearly monodisperse CaWO 4 and CaWO 4 :Eu 3 þmicrospheres have been synthesized in largescale by a surfactant-assisted solution route, in which cetyltrimethyl ammonium bromide(CTAB) is used. X-ray di®raction (XRD), scanning electron microscopy (SEM), Fourier trans-form infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and photo-luminescence (PL) were used to characterize the resulting samples. The results of XRD indicatethat the CaWO 4 and CaWO 4 :Eu 3 þsamples have the scheelite structures. The growth process ofthese nearly monodisperse spheres with an average diameter around 3.2 ? m has been examined. The results of FTIR indicate that CTAB plays an important role in the formation of micro-spheres. The CaWO 4 microspheres exhibit a blue emission band with a maximum at 423 nm. Butthe CaWO 4 :Eu 3 þmicrospheres exhibit a red emission band with a maximum at 623 nm.
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Study on morphology and photoluminescence of Au/SiO2 nanocomposite films
Aichun Yang,Yuguo Li,Boshi Zhuo,Ruiqin Peng,Xuelei Zheng 한국물리학회 2012 Current Applied Physics Vol.12 No.1
Au/SiO2 nanocomposite films were fabricated on Si (111) substrates by radio frequency (RF) magnetron sputtering technique and annealing at different temperature for 20 min (mode A) and at 1000℃ for different annealing time (mode B). The nanocomposite films were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and photoluminescence (PL). SEM results demonstrate that the size of Au crystallites in mode A first increases and then decreases, on increasing annealing temperature, according to the results of XRD spectra. Analysis of PL spectra in mode B shows that the intensity of the emission peak at 440 nm and 523 nm early increases and late decreases, with increasing annealing time at 1000℃. The origin of the emission peak at around 440 nm was related to the size and quantity of Au particles and one of the emission peak at around 523 nm was related to the nanostructure of films in agreement with SEM imagines. Experimental results indicated that morphology, microstructure and luminescence of Au/SiO2 nanocomposite films showed close affinity with annealing temperature and annealing time.
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Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet
YingWu Shi,Chun Li,HongMei Yang,Tao Zhang,Yan Gao,Min Chu,Jun Zeng,Qing Lin,OuTiKuEr,YuGuo Li,Xiangdong Huo,Kai Lou 한국미생물학회 2017 The journal of microbiology Vol.55 No.4
This study details the introduction of a gfp marker into anendophytic bacterial strain (Achromobacter marplatensisstrain 17, isolated from sugar beet) to monitor its colonizationof sugar beet (Beta. vulgaris L.). Stability of the plasmidencoding the gfp was confirmed in vitro for at least 72 h ofbacterial growth and after the colonization of tissues, undernonselective conditions. The colonization was observed usingfluorescence microscopy and enumeration of culturableendophytes in inoculated sugar beet plants that grew for 10or 20 days. gfp-Expressing strains were re-isolated from theinner tissues of surface-sterilized roots and stems of inoculatedplants, and the survival of the Achromobacter marplatensis17:gfp strain in plants 20 days after inoculation, evenin the absence of selective pressure, suggests that it is goodcolonizer. These results also suggest that this strain could bea useful tool for the delivery of enzymes or other proteinsinto plants. In addition, the study highlights that sugar beetplants can be used effectively for detailed in vitro studies onthe interactions between A. marplatensis strain 17 and its host,particularly if a gfp-tagged strain of the pathogen is used.
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Wu Jiang,Fu Liwei,Yan Zineng,Yang Yu,Yin Han,Li Pinxue,Yuan Xun,Ding Zhengang,Kang Teng,Tian Zhuang,Liao Zhiyao,Tian Guangzhao,Ning Chao,Li Yuguo,Sui Xiang,Chen Mingxue,Liu Shuyun,Guo Quanyi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more endogenous stem cells from the joint cavity to the defect area to promote cartilage regeneration.In this study, we used ECM alone as a bioink in low-temperature deposition manufacturing (LDM) 3D printing and then successfully fabricated a hierarchical porous ECM scaffold incorporating GDF-5.Comparative in vitro experiments showed that the 7% ECM scaffolds had the best biocompatibility. After the addition of GDF-5 protein, the ECM scaffolds significantly improved bone marrow mesenchymal stem cell (BMSC) migration and chondrogenic differentiation. Most importantly, the in vivo results showed that the ECM/GDF-5 scaffold significantly enhanced in situ cartilage repair.In conclusion, this study reports the construction of a new scaffold based on the concept of in situ regeneration, and we believe that our findings will provide a new treatment strategy for AC defect repair.
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