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- 저자 : Xiangpeng Gao (검색결과 3 건)
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Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse
Han, Haoyuan,Zhang, Qin,Gao, Kexin,Yue, Xiangpeng,Zhang, Tao,Dang, Ruihua,Lan, Xianyong,Chen, Hong,Lei, Chuzhao Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8
In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five YSNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (${\pi}=5.6{\times}10^{-4}$) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (${\pi}=0.00000$) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.
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Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse
Haoyuan Han,Qin Zhang,Kexin Gao,Xiangpeng Yue,Tao Zhang,Rui-Hua Dang,Xianyong Lan,Hong Chen,Chuzhao Lei 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8
In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five Y-SNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (π = 5.6×10‒4) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (π = 0.00000) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.
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Shaojie Wu,Mingyang Li,Lili Xin,Hongming Long,Xiangpeng Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-
Conventional photocatalysts for environmental applications are mainly transitional metal compounds orprecious metal nanoparticles, which suffer from weak pollutant adsorption performance, thus limitedtheir applications for high concentrated wastewaters. In this study, asynchronous cross-linked triethylenetetraminemodified sodium alginate (CFTS) was used as photocatalyst for the simultaneouslycatalytic degradation of Cr(VI) and metronidazole (MNZ). Experimental results suggested that asynchronouscross-linking and triethylenetetramine modification have dramatically enhanced the chemicalstability as well as the adsorption and photocatalytic behavior of the material. The grafted amino functionalgroups and cross-linked Fe3+ ions can promote the rapid separation and transfer of electrons, thusreducing the recombination rate of photo-generated electron-hole pairs. The optimum removal rate of Cr(VI) has reached 99.5 % and MNZ can be completely removed within 20 and 30 min at pH 1.0 under UVlight irradiation, respectively. The removal mechanism of Cr(VI) by CFTS is the synergistic effect of electrostaticadsorption and photocatalytic reduction, while photocatalytic oxidation dominated the degradationprocess of MNZ. Moreover, the removal efficiency can be enhanced in the binary solution ofthese two pollutants due to the utilization of photo generated electron-hole pairs, which provides newpathways in the treatment of complicated pharmaceutical and domestic sewage wastewaters.
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