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Loss of Stemness by Suppression of Kruppel-Like Factor 5 in Human Limbal Stem Cells
( Woo June Hur ),( Jun Sub Choi ),( Choun Ki Joo ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
The aim of this study is to investigate the role of endogenous Kruppel-like factor 5 (KLF5) in human limbal stem cells (hLSCs). The hLSCs were isolated from the corneoscleral rims of donors after corneal transplantation. Isolated cells were co-cultured with mitomycin-c (MMC)-treated fibroblasts. HLSCs were treated with siRNA directed against KLF5, and effects on stemness were analyzed using colony forming efficacy (CFE). ABCG2, OCT4 and P63 as markers of hLSCs, cytokeratin 3 (CK3) and E-cadherin as markers of differentiation were confirmed by RT-PCR, western blotting assay, immunocytochemistry. And corneal epithelialization of the limbal stem cells was confirmed by air-lift 3d-culture. The number of colony (larger than 1 mm in diameter) in KLF5 siRNA treated-group was observed lower than that of control groups (non-treated group and negative siRNA treated-group). And expression of PCNA, OCT4, ABCG2 and P63 were decreased by KLF5 siRNA treatment. On the other hand, expression of cyclin D1, CK3 and E-cadherin were increased in KLF5 siRNA treated-group. Additionally, the limbal stem cells with klf5 siRNA treatment had not formed multi-layers in air-lift 3d-culture. In this study, suppression of KLF5 expression in hLSCs induced the loss of their stemness. These results suggested that KLF5 is closely relative to proliferation and stemness of hLSCs in cornea.
Yoon, Chang-Hwan,Hur, Jin,Oh, Il-Young,Park, Kyung-Woo,Kim, Tae-Youn,Shin, Jae-Hoon,Kim, Ji-Hyun,Lee, Choon-Soo,Chung, June-Key,Park, Young-Bae,Kim, Hyo-Soo Ovid Technologies Wolters Kluwer -American Heart A 2006 Arteriosclerosis, thrombosis, and vascular biology Vol.26 No.5
<P>BACKGROUND: Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules. METHODS AND RESULTS: Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and platelet-endothelial cell adhesion molecule-1 (PECAM-1) in ischemic muscle by immunofluorescence. ICAM-1 was overexpressed in ischemic muscle compared with nonischemic muscle, whereas expressions of E-selectin, VCAM-1, and PECAM-1 did not show that much difference. ICAM-1 was also upregulated by hypoxia in murine endothelial cells (ECs) as assessed by immunoblot and flow cytometry. EPCs were attached to ECs specifically through ICAM-1/beta-2 integrin interaction in vitro. When EPCs were labeled with fluorescent dye or radioisotope (Tc-99m-HMPAO) and systemically administrated in vivo, EPCs preferentially homed to ischemic muscle. By blocking ICAM-1, EPCs entrapment to ischemic limb in vivo was significantly reduced and neovascularization induced by EPC transplantation was attenuated. CONCLUSIONS: ICAM-1 is upregulated by ischemia, and this is closely associated with EPCs entrapment to ischemic limb. Our findings suggest that ICAM-1 expression might be important in regulating the process of neovascularization through its ability to recruit EPCs.</P>
Hyunmin Choe,Gam Hur,Woo-Ik Jang,Chang Young Kim,Sung Uk Kwon,Joon Hyung Doh,June Namgung,Sung Yun Lee,Won Ro Lee 연세대학교의과대학 2009 Yonsei medical journal Vol.50 No.1
A coronary artery aneurysm is an uncommon disorder and is seen as a characteristic dilatation of a localized portion of the coronary artery. Clinical manifestation of a coronary artery aneurysm varies from an asymptomatic presentation to sudden death of a patient. Although coronary aneurysms are typically diagnosed by the use of coronary angiography, a new generation of coronary 64-slice multidetector computed tomography (64- MDCT) scanners have successfully been used for evaluating this abnormality in a noninvasive manner. In the present case, we performed coronary 64-MDCT scanning preoperatively and postoperatively on a patient with multiple giant coronary aneurysms. The use of coronary 64-MDCT may provide an evaluation technique not only for diagnosis but also for follow-up after surgery for this condition.
(Hydroxyapatite 코팅 대퇴 삽입물을 이용한 인공 고관절 전 치환술 -최소 5년 이상의 추시결과-
한창동 ( Chang Dong Han ),이우석 ( Woo Suk Lee ),허준혁 ( June Huyck Hur ) 대한고관절학회 1998 Hip and Pelvis Vol.10 No.2
Forty-two primary total hip arthroplasty with hydroxyapatite-coated femoral stem were performed in thirty-seven patients from 1992 to 1993. The patients were followed for a minimum of 5 years and the results were evaluated with clinical and radiological data. The mean Harris hip score increased from 60.8 points preoperatively to 93.3 points at the time of the most recent follow-up, and 93% of patients had good or excellent results. On radiologic examination, there was a rapid bony integration of implants with bone apposition on the coating within 6 months. Endosteal bone formation(spotwelds) was mainly seen at Gruen zone 2(71%) and 6(74%) which were the area with hydroxyapatite coating. Radiolucent lines were mainly detected at the uppermost portion of Gruen zone 1(86%). None of the femoral stems showed evidence of measurable migration and all femoral stems were radiologically stable. The progressive osteolysis was most frequently of the linear type which was seen from Gruen zone 1, and extended to Gruen zone 2. The rate of progressive linear osteolysis was relatively low(7.1%) and no distal osteolysis was found. The hydroxyapatite-coated femoral stems have produced encouraging results in these patients, although longer follow-up is necessary to determine its durability.
In Seok Park,Jun Wook Hur,Soo Yeon Im,Dong Won Seol,Woo June Hur,Min Ouk Park,Hye Suck An,Eun Mi Kim,Seok Jung Han 한국유전학회 2008 Genes & Genomics Vol.30 No.2
Morphometric traits, percent shell weight, percent cavity volume and condition index of 4-year-old induced hybrid abalone from female Haliotis gigantea Gmelin and male Haliotis discus discus Reeve were compared to those of the parental species. Apex height/shell height of the induced hybrid abalone was in between the average height of the parental species. The induced hybrid abalone shared three traits with the paternal species and two with the maternal species. After 4 years of growth, the condition index of the induced hybrids weresignificantly higher than that of either parental species ( P< 0.05). Generally, induced hybrid abalone displayed an external morphology that was intermediate between the representative of both parental species. Haliotis gigantea and the induced hybrids had four respiratory pores, while H. discus discus had five.
Dong Won Seol,Soo Yeon Im,Woo June Hur,Min Ouk Park,Dong Soo Kim,Jae Yoon Jo,In Seok Park 한국유전학회 2008 Genes & Genomics Vol.30 No.3
Haematological features pertaining to aerobic capability were compared between diploid and triploid specimens of the Far Eastern catfish, Silurus asotus. No significant differences between diploids and triploids were found for the haematocrit value, total haemoglobin, and mean corpuscular haemoglobin concentration, while the mean corpuscular volume, mean corpuscular haemoglobin, and plasma glucose concentration were significantly higher in triploids than in diploids, and the number of red blood cells was significantly lower in triploids than in diploids. The oxygen consumption rate did not differ significantly between diploid and triploid fish ( P>0.05). Nevertheless, the respiratory frequency was higher in triploids than in diploids ( P< 0.05). Triploids were characterized by a lower concentration of circulating blood cells, and aquaculture practice should consider the need for a lower surface to volume ratio.
Lee, Hae-Youn,Kim, Jinyoung,Quan, Wenying,Lee, June-Chul,Kim, Min-Soo,Kim, Seok-Hyung,Bae, Jin-Woo,Hur, Kyu Yeon,Lee, Myung-Shik Informa UK (TaylorFrancis) 2016 Autophagy Vol.12 No.8
<P>Autophagy, which is critical for the proper turnover of organelles such as endoplasmic reticulum and mitochondria, affects diverse aspects of metabolism, and its dysregulation has been incriminated in various metabolic disorders. However, the role of autophagy of myeloid cells in adipose tissue inflammation and type 2 diabetes has not been addressed. We produced mice with myeloid cell-specific deletion of Atg7 (autophagy-related 7), an essential autophagy gene (Atg7 conditional knockout [cKO] mice). While Atg7 cKO mice were metabolically indistinguishable from control mice, they developed diabetes when bred to ob/w mice (Atg7 cKO-ob/ob mice), accompanied by increases in the crown-like structure, inflammatory cytokine expression and inflammasome activation in adipose tissue. M phi s (macrophages) from Atg7 cKO mice showed significantly higher interleukin 1 release and inflammasome activation in response to a palmitic acid plus lipopolysaccharide combination. Moreover, a decrease in the NAD(+):NADH ratio and increase in intracellular ROS content after treatment with palmitic acid in combination with lipopolysaccharide were more pronounced in M phi s from Atg7 cKO mice, suggesting that mitochondrial dysfunction in autophagy-deficient M phi s leads to an increase in lipid-induced inflammasome and metabolic deterioration in Atg7 cKO-ob/ob mice. Atg7 cKO mice were more susceptible to experimental colitis, accompanied by increased colonic cytokine expression, T helper 1 skewing and systemic bacterial invasion. These results suggest that autophagy of M phi s is important for the control of inflammasome activation in response to metabolic or extrinsic stress, and autophagy deficiency in M phi s may contribute to the progression of metabolic syndrome associated with lipid injury and colitis.</P>