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      • SCIESCOPUSKCI등재

        Characterization of Lipases from Staphylococcus aureus and Staphylococcus epidermidis Isolated from Human Facial Sebaceous Skin

        ( Xie Winny ),( Vivia Khosasih ),( Antonius Suwanto ),( Hyung Kwoun Kim ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

        Two staphylococcal lipases were obtained from Staphylococcus epidermidis S2 and Staphylococcus aureus S11 isolated from sebaceous areas on the skin of the human face. The molecular mass of both enzymes was estimated to be 45 kDa by SDS-PAGE. S2 lipase displayed its highest activity in the hydrolysis of olive oil at 32oC and pH 8, whereas S11 lipase showed optimal activity at 31oC and pH 8.5. The S2 lipase showed the property of cold-adaptation, with activation energy of 6.52 kcal/mol. In contrast, S11 lipase`s activation energy, at 21 kcal/mol, was more characteristic of mesophilic lipases. S2 lipase was stable up to 45oC and within the pH range from 5 to 9, whereas S11 lipase was stable up to 50oC and from pH 6 to 10. Both enzymes had high activity against tributyrin, waste soybean oil, and fish oil. Sequence analysis of the S2 lipase gene showed an open reading frame of 2,067 bp encoding a signal peptide (35 aa), a pro-peptide (267 aa), and a mature enzyme (386 aa); the S11 lipase gene, at 2,076 bp, also encoded a signal peptide (37 aa), pro-peptide (255 aa), and mature enzyme (399 aa). The two enzymes maintained amino acid sequence identity of 98-99% with other similar staphylococcal lipases. Their microbial origins and biochemical properties may make these staphylococcal lipases isolated from facial sebaceous skin suitable for use as catalysts in the cosmetic, medicinal, food, or detergent industries.

      • KCI등재

        Volumetric measurement of the tongue and oral cavity with cone-beam computed tomography: A systematic review

        Aflah Kannitha Alina,Yohana Winny,Oscandar Fahmi 대한영상치의학회 2022 Imaging Science in Dentistry Vol.52 No.4

        Purpose: The goal of this systematic review was to compare the use of cone-beam computed tomography (CBCT) with that of computed tomography (CT) for volumetric evaluations of the tongue and oral cavity. Materials and Methods: A search for articles was conducted in accordance with the Preferred Reporting Items for Systematic Review and Meta-analyses guidelines. The PubMed, Scopus, ScienceDirect, and SAGE Journals databases were searched for articles published between 2011 and 2021. Articles were screened and assessed for eligibility. Screening involved checking for duplication, reading the title and abstract, and reading the full text. Results: The initial search retrieved 25,780 articles. Application of the eligibility criteria yielded 16 articles for qualitative analysis. Multiple uses of CBCT were identified. In several studies, researchers assessed the volumetric correlation between tongue and oral cavity volumes, as well as other parameters. Post-treatment volumetric evaluations of the oral cavity were also reported, and the reliability of CBCT was assessed. The use of CT resembled that of CBCT. Conclusion: CBCT has been used in the evaluation of tongue and oral cavity volumes to assess correlations between those volumes and with the upper airway. It has also been used for volumetric evaluation after surgical and non- surgical procedures and to assess the relationships between tongue volume, tooth position, occlusion, and body mass index. Participants with obstructive sleep apnea and malocclusion have been evaluated, and the reliability of CBCT has been assessed. In the included studies, CT was utilized for similar purposes as CBCT, but its reliability was not assessed.

      • SCOPUSKCI등재

        Volumetric measurement of the tongue and oral cavity with cone-beam computed tomography: A systematic review

        Kannitha Alina, Aflah,Winny, Yohana,Fahmi, Oscandar Korean Academy of Oral and Maxillofacial Radiology 2022 Imaging Science in Dentistry Vol.52 No.-

        Purpose: The goal of this systematic review was to compare the use of cone-beam computed tomography (CBCT) with that of computed tomography (CT) for volumetric evaluations of the tongue and oral cavity. Materials and Methods: A search for articles was conducted in accordance with the Preferred Reporting Items for Systematic Review and Meta-analyses guidelines. The PubMed, Scopus, ScienceDirect, and SAGE Journals databases were searched for articles published between 2011 and 2021. Articles were screened and assessed for eligibility. Screening involved checking for duplication, reading the title and abstract, and reading the full text. Results: The initial search retrieved 25,780 articles. Application of the eligibility criteria yielded 16 articles for qualitative analysis. Multiple uses of CBCT were identified. In several studies, researchers assessed the volumetric correlation between tongue and oral cavity volumes, as well as other parameters. Post-treatment volumetric evaluations of the oral cavity were also reported, and the reliability of CBCT was assessed. The use of CT resembled that of CBCT. Conclusion: CBCT has been used in the evaluation of tongue and oral cavity volumes to assess correlations between those volumes and with the upper airway. It has also been used for volumetric evaluation after surgical and nonsurgical procedures and to assess the relationships between tongue volume, tooth position, occlusion, and body mass index. Participants with obstructive sleep apnea and malocclusion have been evaluated, and the reliability of CBCT has been assessed. In the included studies, CT was utilized for similar purposes as CBCT, but its reliability was not assessed.

      • KCI등재

        A Simple Interactive 3D Interior Design Application for Living Room - Cost Management

        Winnie, Chin,Hui, Saw Seow,Gee, Yap Seok Korea Multimedia Society 2017 The journal of multimedia information system Vol.4 No.3

        The title of this project is "A Simple Interactive 3D Interior Design Application for Living Room - Cost Management", focusing on developing an application that enables costing and designing to be executed concurrently in interior design phases. This is because any changes made at costing phase after design phase is completed, it will potentially lead to unnecessary increase in cost and time. Therefore, the objective of this project is aimed to devise a cost management module to be included in the proposed application, allowing interior designer to make cost-based decision at the designing phase itself. This can be achieved through its proposed functions which include total cost update, budget management, cost estimator calculator and material list generation. The methodology being proposed in this project is Prototyping Development, categorized under Rapid Application Development (RAD), by starting off with planning, analysis, design and implementation phases are performed concurrently and repeatedly in a cycle to produce a prototype until the application is completed. In the end of this project, the developed prototype is expected to contribute a better solution towards cost management in interior design.

      • Utility Specialists in Hong Kong : A Corpus Study of Perception of Communicative Competence

        Winnie Cheng 한국코퍼스언어학회 2015 Corpus Linguistics Research Vol.1 No.-

        The paper reports on part of a larger scale collaborative and interdisciplinary project among English Applied Studies, Land Surveying and Geo-informatics, and utility specialists industry in engineering in Hong Kong. The present study interviewed 36 utility specialists in Hong Kong to find out their understanding of and perceived importance of communicative competences in English situated in their professional workplaces. The corpora created from the interview data were analysed in several complementary and inter-connected ways, namely word lists, concgram lists, and key semantic fields, to ascertain the kinds of meanings or topical concerns and themes expressed by the professionals when they talked about different types of competences in communication, namely linguistic, sociolinguistic, discourse, strategic, socio-cultural, and social in their workplace. The project has also achieved effective outcomes in terms of knowledge sharing and exchange with the industry.

      • KCI등재

        The Roles of Menopausal-specific Quality of Life on Breast Cancer Screening Beliefs in Menopausal and Postmenopausal Women

        Winnie Lai-Sheung Cheng 대한폐경학회 2018 대한폐경학회지 Vol.24 No.3

        Objectives: This study investigated the association between menopausal-specific quality of life (MENQOL) and breast cancer screening beliefs among Hong Kong Chinese menopausal and postmenopausal women.Methods: A cross-sectional study was employed to collect data. The questionnaire was distributed to a convenience sample of 218 women aged above 45. The outcome variables were breast cancer screening beliefs and breast examination. Multiple regression analyses were conducted to test the effect of 4 domains in MENQOL (vasomotor, psycho-social, physical, and sexual symptom) and the health behaviors on the 2 outcome variables.Results: This study found that the overall MENQOL scores (in particular psycho-social, and physical aspects) were significantly associated with positive attitudes toward health check-ups and better knowledge and perceptions in breast cancer. Regular exercise was also significantly related to breast examination.Conclusions: MENQOL (especially psycho-social and physical domain) and regular exercise are important factors associated with breast cancer screening beliefs. The results of this study illuminate health care professionals to develop primary health care strategies to improve the quality of life of mid-life women.

      • SCIESCOPUSKCI등재

        Does Acupuncture Therapy Alter Activation of Neural Pathway for Pain Perception in Irritable Bowel Syndrome?: A Comparative Study of True and Sham Acupuncture Using Functional Magnetic Resonance Imaging

        ( Winnie Cw Chu ),( Justin Cy Wu ),( David Tw Yew ),( Liang Zhang ),( Lin Shi ),( David Kw Yeung ),( De Feng Wang ),( Raymond Ky Tong ),( Ya Wen Chan ),( Li Xing Lao ),( Ping C Leung ),( Brian M Berma 대한소화기기능성질환·운동학회 2012 Journal of Neurogastroenterology and Motility (JNM Vol.18 No.3

        Background/Aims Patients with irritable bowel syndrome (IBS) are characterized by abnormal central processing with altered brain activation in response to visceral nociceptive signals. The effect of electroacupuncture (EA) on IBS patients is unclear. The study is set to study the effect of EA on brain activation during noxious rectal distension in IBS patients using a randomized sham-controlled model. Methods Thirty IBS-diarrhea patients were randomized to true electroacupuncture or sham acupuncture. Functional MRI was performed to evaluate cerebral activation at the following time points: (1) baseline when there was rectal distension only, (2) rectal distension during application of EA, (3) rectal distension after cessation of EA and (4) EA alone with no rectal distension. Group comparison was made under each condition using SPM5 program. Results Rectal distension induced significant activation of the anterior cingulated cortex, prefrontal cortex, thalamus, temporal regions and cerebellum at baseline. During and immediately after EA, increased cerebral activation from baseline was observed in the anterior cingulated cortex, bilateral prefrontal cortex, thalamus, temporal regions and right insula in both groups. However, true electroacupuncture led to significantly higher activation at right insula, as well as pulvinar and medial nucleus of the thalamus when compared to sham acupuncture. Conclusions We postulate that acupuncture might have the potential effect of pain modulation in IBS by 2 actions: (1) modulation of serotonin pathway at insula and (2) modulation of mood and affection in higher cortical center via ascending pathway at the pulvinar and medial nucleus of the thalamus. (J Neurogastroenterol Motil 2012;18:305-316)

      • Green Activism in the City: Hong Kong Independent Ecofilms in the Post-2008 Era

        Winnie L M YEE 연세대학교 영어영문학과 BK21 Plus 사업단 2018 Situations: Cultural Studies in the Asian Context Vol.11 No.1

        More and more people are acutely aware of the pollutants entering the city from the industries in Southern China, more people are “going green,” and discussions of animal rights have become much more prominent. Still, the lack of imminent danger seems to have lulled the citizens of Hong Kong into a comfortable sense that they are, at least temporarily, free from global climate change. Two arenas of ecocritical responsibility will be examined in this paper: alternative communities, with a focus on Sangwoodgoon, and the thriving ecocinema scene in Hong Kong. Sangwoodgoon combines farming activities with films, art, and performance, creating a platform that connects the aesthetic portrayal of the land with the physical working of the land. It seeks to engage audiences with the land, both as a physical site and as a rich terrain of the imagination. The combination of art appreciation and farm-related activities encourages members to reflect on life and environment, nature and development, the local and the globe.

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