Targeting the IL-1β/IL-1Ra pathways for the aggregation of human islet amyloid polypeptide in an ex vivo organ culture system of the intervertebral disc

Cao Yang,Xinghuo Wu,Zhiwei Liao,Kun Wang,Wenbin Hua,Xianzhe Liu,Yu Song,Yukun Zhang,Shuhua Yang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Intervertebral disc degeneration (IDD) is characterized by excessive apoptosis of nucleus pulposus (NP) cells and hyperactive extracellular matrix (ECM) catabolism. Our previous studies revealed the relationship between human islet amyloid polypeptide (hIAPP) and NP cell apoptosis. However, the role of hIAPP aggregates in IDD has not yet been investigated. This study aimed to determine whether the accumulation of hIAPP aggregates promotes IDD progression. The aggregation of hIAPP increased in human NP tissues during IDD. The deposition of hIAPP aggravated the compression-induced IDD that promoted NP cell apoptosis and ECM degradation via IL-1β/IL-1Ra signaling in an ex vivo rat disc model. Moreover, neutralizing IL-1β augmented the protective effects of hIAPP overexpression by decreasing hIAPP aggregation in human NP cells. These results suggest that the aggregation of hIAPP promotes NP cell apoptosis and ECM degradation ex vivo and in vitro by disrupting the balance of IL-1β/IL-1Ra signaling.

Performance study of g-C3N4/carbon black/BiOBr@Ti3C2/MoS2 photocatalytic fuel cell for the synergistic degradation of different types of pollutants

Guo Huilin,Yu Tingting,Zhao Lei,Qian Jun,Yu Jiahe,Zhang Yu,Teng Yongyue,Zhu Chunshui,Yang Tao,Chen Wenbin,Gong Picheng,Jiang Cuishuang,Gao Changfei,Yang Bing,Yang Chenyu 한국탄소학회 2023 Carbon Letters Vol.33 No.3

In this study, a bipolar visible light responsive photocatalytic fuel cell (PFC) was constructed by loading a Z-scheme g-C3N4/carbon black/BiOBr and a Ti3C2/MoS2 Schottky heterojunction on the carbon brush to prepare the photoanode and photocathode, respectively. It greatly improved the electron transfer and achieved efficient degradation of organic pollutants such as antibiotics and dyes simultaneously in two chambers of the PFC system. The Z-scheme g-C3N4/carbon black/BiOBr formed by adding highly conductive carbon black to g-C3N4/BiOBr not only effectively separates the photogenerated carriers, but also simultaneously retains the high reduction of the conduction band of g-C3N4 and the high oxidation of the valence band of BiOBr, improving the photocatalytic performance. The exceptional performance of Ti3C2/MoS2 Schottky heterojunction originated from the superior electrical conductivity of Ti3C2 MXene, which facilitated the separation of photogenerated electron–hole pairs. Meanwhile, the synergistic effect of the two photoelectrodes further improved the photocatalytic performance of the PFC system, with degradation rates of 90.9% and 99.9% for 50 mg L−1 tetracycline hydrochloride (TCH) and 50 mg L−1 rhodamine-B (RhB), respectively, within 180 min. In addition, it was found that the PFC also exhibited excellent pollutant degradation rates under dark conditions (79.7%, TCH and 97.9%, RhB). This novel pollutant degradation system is expected to provide a new idea for efficient degradation of multiple pollutant simultaneously even in the dark.

Electromagnetic Counter-recoil Mechanism Based on Adaptive Sliding Mode Control

Wenbin Yu,Guolai Yang,Liqun Wang,Lei Li,Hongyi Zhang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.9

Traditional counter-recoil machines always have problems of difficulty in adjusting the movement process flexibly, maintenance difficulties, and so on. To end these, this paper proposes a new electromagnetic counterrecoil scheme using cylindrical linear motors. Firstly, the mathematical expression of counter-recoil movement under the linear motor is formulated, and the ideal movement trajectory is designed using the piecewise polynomial of velocity based on acceleration. To obtain a better performance, of the motor controller, a composed approach of an adaptive sliding mode control based on barrier function (BFASMC) and the finite-time disturbance observer (FTDO) is introduced into the modeling and computation. The stability of the controller is proved by establishing the Lyapunov function. The new adaptive laws based on the barrier function effectively make up for the shortcomings of the exponential function and improves control chattering. At last, the new scheme is simulated. The results show that it has a strong robustness and anti-interference ability.

Molecular characterization of a sweet potato stress toleranceassociated trehalose-6-phosphate synthase 1 gene (IbTPS1) in response to abiotic stress

Wenbin Wang,Huan Yu,김호수,Yanxin Yang,Xiangpo Qiu,곽상수 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

Trehalose-6-phosphate synthase (TPS) plays an important regulatory role in the response of plants to multiple abiotic stresses. However, our knowledge of the stress tolerance functions of TPS genes in sweet potato (Ipomoea batatas [L.] Lam) remains limited. In the present study, we isolated and functionally characterized the sweet potato gene encoding TPS1, IbTPS1. Sequence analysis showed that IbTPS1 belongs to class I TPS proteins and harbors highly conserved acceptor (glucose- 6-phosphate [Glc6P]) and donor (uridine diphosphoglucose [UDP-Glc]) binding sites. The IbTPS1 gene showed the highest level of constitutive expression in leaves and storage roots of sweet potato plants and was induced by several environmental stresses including drought, salt, and heat shock. The IbTPS1 protein might be localized to the cytosol. Complementation assay of yeast tps1Δ and tps1Δtps2Δ growth-defective mutants confirmed the TPS activity of IbTPS1, and truncation of the N-terminal extension of IbTPS1 (ΔNIbTPS1) increased the catalytic activity of the protein. Additionally, expression of IbTPS1 in yeast conferred abiotic stress tolerance to dehydration, salinity, and oxidation, and expression of ΔNIbTPS1 further enhanced the tolerance to abiotic stresses. The results of this study advance our understanding of the functions of IbTPS1 under abiotic stress conditions. Our results suggest that the IbTPS1 gene is an excellent candidate for improving the stress tolerance of different crop plants.

Evaluation of Public Servant Execution Based on Data Mining Technique and Multiple Factors Joint Modeling Analysis

Yang Du,Wenbin Chen,Di Cheng 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.1

With the rapid development of computer science and technology, data mining modelling techniques have emerged and rapidly developed as an alternative powerful meta-learning tool to accurately and fast analyze the massive volume of data generated by modern applications. The combination of data analysis technique and evaluation of public servant execution is urgently needed. Improve the execution of public servants at the grass-roots level is one of the important link to strengthen the construction of authority administrative efficiency of administrative goals is very important. Enhance the execution must first cultivate advanced concept, armed with advanced execution concept to the vast number of public servants at the grass-roots level. The assessment of public execution has a lot of traditional methods and models can be used but there is limitation. The limitation could be concluded as the following. Carelessness or poor sensitivity, At the grassroots level, the implementation of the main body of the general public servants at the grass-roots level and they can perform in place, one of the important factor is whether the leader on the work division of labor, organization, management and supervision effectively. In this paper, we conduct research on evaluation of public servant execution based on data mining technique and joint modeling analysis of multiple factors under big data environment. Firstly, we introduce some state-of-the-art clustering algorithm to serve as the basis of our model. Combined with deep neural network and optimization modelling, we propose our support vector machine based data clustering algorithm through multiple factor modelling. Subsequently, we discuss the principles on evaluation of public servant execution and process management. In the experimental part, we conduct experiment on both data clustering based data pre-processing step and the evaluation of elements’ weight for process management. The result indicates the most important factor for management and the feasibility and effectiveness of our proposed clustering method. Future potential research areas are also discussed in the final Section.

Application of Optic Emission Spectroscopy in Plasma Electrolytic Oxidation

Wenbin Xue,Run Liua,Xuan Yang,Xiaoyue Jin,Jiancheng Du 한국표면공학회 2017 한국표면공학회 학술발표회 초록집 Vol.2017 No.11

A tubular linear motor structure suitable for large thrust

Wenbin Yu,Guolai Yang,Zixuan Li,Dianrong Wang,Xiuye Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.11

In response to the problem that the motor shaft of conventional tubular linear motor is prone to bending, this paper proposes a new tubular linear motor structure for large thrust demand. Bending of the motor shaft can lead to collisions between structures and affect motor performance, and traditional air-gap structures cannot effectively handle this problem. The stator of the new motor is divided into an inner and outer cylinder structure. The armature windings are arranged between the inner and outer cylinders. The permanent magnets are arranged on the motor shaft. After analysis, the structural proposed in this paper can well stop the bending of the motor shaft. It also effectively improves the problem of large fluctuations in the thrust of linear motors. Finally, it is concluded that the peak motor thrust is maximized when the material with low permeability is chosen for the inner cylinder.

Molecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress

Wenbin Wang,Xiangpo Qiu,Ho Soo Kim,Yanxin Yang,Dianyun Hou,Xuan Liang,곽상수 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Dehydroascorbate reductase (DHAR) plays a critical role in the regeneration of ascorbic acid (AsA), and widely involved in plant tolerance to biotic and abiotic stresses. In this study, the IbDHAR3 gene was cloned from sweetpotato cultivar Xushu 18 by RT-PCR. The full-length of this gene was 813 bp which encodes 270 amino acids. The IbDHAR3 protein contained two conserved domains of glutathione S-transferase (GST) and GST-C-DHAR, and one chloroplast transit peptide with 52 amino acids length. Transient expression in tobacco leaf epidermal cells indicated that IbDHAR3 protein is subcellular localized to chloroplast. The qRT-PCR results revealed that the relative expression level of IbDHAR3 in leaves is much higher than that in other tissues, and could be up-regulated by ABA, drought, salinity, and high-temperature stresses. The seed germination rate and root elongation were increased in contrast to wild type under mannitol and NaCl stresses in T3 transgenic Arabidopsis overexpressing IbDHAR3 gene. The soil drought experiments showed that the overexpression of IbDHAR3 gene in Arabidopsis reduced the malondialdehyde (MDA) and the H2O2 content, enhanced the level of AtGR gene expression, superoxide dismutase (SOD), ascorbate peroxidase (APX), DHAR activity, and the AsA content. Therefore, overexpression of IbDHAR3 gene could enhance the ability of scavenging reactive oxygen species such as H2O2 by promoting AsA-glutathione cycle and related antioxidant enzymes system, thereby contributing to increased stress tolerance in Arabidopsis.

Effects of ruminally degradable starch levels on performance, nitrogen balance, and nutrient digestibility in dairy cows fed low corn-based starch diets

Guobin Luo,Wenbin Xu,Jinshan Yang,Yang Li,Liyang Zhang,Yizhen Wang,Cong Lin,Yonggen Zhang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.5

Objective: This trial was performed to examine the effects of ruminally degradable starch (RDS) levels in total mixed ration (TMR) with low corn-based starch on the milk production, whole-tract nutrient digestibility and nitrogen balance in dairy cows. Methods: Eight multiparous Holstein cows (body weight [BW]: 717±63 kg; days in milk [DIM]: 169±29) were assigned to a crossover design with two dietary treatments: a diet containing 62.3% ruminally degradable starch (% of total starch, low RDS) or 72.1% ruminally degradable starch (% of total starch, high RDS). Changes to the ruminally degradable levels were conducted by using either finely ground corn or steam-flaked corn as the starch component. Results: The results showed that dry matter intake, milk yield and composition in dairy cows were not affected by dietary treatments. The concentration of milk urea nitrogen was lower for cows fed high RDS TMR than low RDS TMR. The whole-tract apparent digestibility of neutral detergent fiber, acid detergent fiber and crude protein decreased, and that of starch increased for cows fed high RDS TMR over those fed low RDS TMR, with no dietary effect on the whole-tract apparent digestibility of dry matter and organic matter. The proportion of urinary N excretion in N intake was lower and that of fecal N excretion in N intake was higher for cows fed high RDS TMR than those fed low RDS TMR. The N secretion in milk and the retention of N were not influenced by the dietary treatments. Total purine derivative was similar in cows fed high RDS TMR and low RDS TMR. Consequently, estimated microbial N flow to the duodenum was similar in cows fed high RDS TMR and low RDS TMR. Conclusion: Results of this study show that ruminally degradable starch levels can influence whole-tract nutrient digestibility and nitrogen balance in dairy cows fed low corn-based starch diets, with no influence on performance.

An Approach for Lactulose Production Using the CotX-Mediated Spore-Displayed β-Galactosidase as a Biocatalyst

Wang, He,Yang, Ruijin,Hua, Xiao,Zhang, Wenbin,Zhao, Wei The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7

Currently, enzymatic synthesis of lactulose, a synthetic prebiotic disaccharide, is commonly performed with glycosyl hydrolases. In this work, a new type of lactulose-producing biocatalyst was developed by displaying β-galactosidase from Bacillus stearothermophilus IAM11001 (Bs-β-Gal) on the surface of Bacillus subtilis 168 spores. Localization of β-Gal on the spore surface as a fusion to CotX was verified by western blot analysis, immunofluorescence microscopy, and flow cytometry. The optimum pH and temperature for the resulting spore-displayed β-Gal was 6.0 and 75℃, respectively. Under optimal conditions, it showed maximum activity of 0.42 U/mg spores (dry weight). Moreover, the spore-displayed CotX-β-Gal was employed as a whole cell biocatalyst to produce lactulose, yielding 8.8 g/l from 200 g/l lactose and 100 g/l fructose. Reusability tests showed that the spore-displayed CotX-β-Gal retained around 30.3% of its initial activity after eight successive conversion cycles. These results suggest that the CotX-mediated spore-displayed β-Gal may provide a promising strategy for lactulose production.