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RISS 인기검색어
- 검색키워드
- 저자 : Tong Mei (검색결과 39 건)
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Tong‑Mei Gao,Shuang‑Ling Wei,Jing Chen,Yin Wu,Feng Li,Li‑Bin Wei,Chun Li,Yan‑Juan Zeng,Yuan Tian,Dong‑Yong Wang,Hai‑Yang Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.1
Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl-1. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellowgreen mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group. Conclusion The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.
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Protective effect of Cordyceps militaris against hydrogen peroxide-induced oxidative stress in vitro
Mei Tong He,Ah Young Lee,Chan Hum Park,Eun Ju Cho 한국영양학회 2019 Nutrition Research and Practice Vol.13 No.4
BACKGROUND/OBJECTIVES: Excessive production of reactive oxygen species (ROS) such as hydroxyl (·OH), nitric oxide (NO), and hydrogen peroxide (H₂O₂) is reported to induce oxidative stress. ROS generated by oxidative stress can potentially damage glial cells in the nervous system. Cordyceps militaris (CM), a kind of natural herb widely found in East Asia. In this study, we investigated the free radical scavenging activity of the CM extract and its neuroprotective effects in H₂O₂-induced C6 glial cells. MATERIALS/METHODS: The ethanol extract of CM (100-1,000 μg/mL) was used to measure DPPH, ·OH, and NO radical scavenging activities. In addition, hydrogen peroxide (H₂O₂)-induced C6 glial cells were treated with CM at 0.5-2.5 μg/mL for measurement of cell viability, ROS production, and protein expression resulting from oxidative stress. RESULTS: The CM extract showed high scavenging activities against DPPH, ·OH, and NO radicals at concentration of 1,000 μg/mL. Treatment of CM with H₂O₂-induced oxidative stress in C6 glial cells significantly increased cell viability, and decreased ROS production. Cyclooxygenase-2 and inducible nitric oxide synthase protein expression was down-regulated in CM-treated groups. In addition, the protein expression level of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK), phospho-c-Jun N-terminal kinase (p-JNK), and phospho-extracellular regulated protein kinases (p-ERK) in H₂O₂-induced C6 glial cells was down-regulated upon CM administration. CONCLUSION: CM exhibited radical scavenging activity and protective effect against H₂O₂ as indicated by the increased cell viability, decreased ROS production, down-regulation of inflammation-related proteins as well as p-p38, p-JNK, and p-ERK protein levels. Therefore, we suggest that CM could play the protective role from oxidative stress in glial cells.
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THE DETECTION OF INSTANTANEOUS DISTORTED CURRENT WITH THREE-DIMENSIONAL SPACE VECTOR
Tong Mei,Tong Jie,Jiang Jingping 전력전자학회 1998 ICPE(ISPE)논문집 Vol.- No.-
Active power filter is a kind of device used for compensating instantaneous reactive and harmonic current in three-phase circuits An essential technology that determines the behavior of an active power filter is the method of detecting the distorted current Using three-dimensional space vectors, this paper describes a simple method for detecting the distorted current without any coordinate transformation The effectiveness of the novel method is verified by the theoretical analysis and simulation.
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Protective role of Cordyceps militaris in Aβ1–42-induced Alzheimer’s disease in vivo
Mei Tong He,이아영,김지현,박찬흠,신유수,조은주 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
According to the ‘‘amyloid cascade hypothesis’’,amyloid-beta (Ab) protein occupied one of the risk factorsof Alzheimer’s disease (AD). Cordyceps militaris (CM)has been reported to exert anti-inflammatory, anti-oxidant,and neuroprotective activities; however, its activity againstcognitive dysfunction has not been studied yet. In thisstudy, the CM ethanol extract was administered with a doseof 100 or 200 mg/kg for 2 weeks, and behavioral assessmentswere performed for learning and memory function inAb1–42-induced AD mice models. Supplementation withCM extract enhanced new route consciousness and novelobject recognition, and in the Morris water maze test, CMadministeredgroups showed less time to reach to the hiddenplatform compared with the control group. Moreover,the CM extract inhibited nitric oxide production and lipidperoxidation in the brain, liver, and kidney. The presentstudy indicated that CM could have the protective rolefrom cognitive impairment and progression of AD.
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무막줄기세포추출물의 H₂O₂에 의해 유도된 치주 세포의 염증 반응 보호 효과
허메이통(Mei Tong He),김지현(Ji Hyun Kim),김영실(Young Sil Kim),박혜숙(Hye Sook Park),조은주(Eun Ju Cho) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.6
대표적인 치주질환인 치주염은 출혈, 통증 및 치아 손실을 초래하며, 산화적 스트레스는 치주염의 주요 원인으로 알려져 있다. 본 연구는 지방조직 유래 무막줄기세포추출물의 H₂O₂ 유도 산화적 손상에 대한 치주염 보호 효과를 확인하고자, 치주인대 섬유모세포(human periodontal ligament fibroblasts; HPLF)를 이용하여 세포 생존율, 염증 및 세포사멸 관련 단백질 발현을 측정하였다. H₂O₂로 산화적 스트레스를 유도한 HPLF 세포에 무막줄기세포추출물 처리 시, H₂O₂만을 처리한 control군에 비해 유의적으로 세포 생존율이 증가함을 통해 산화적 손상에 대한 세포 보호 효과를 확인하였다. 또한, 무막줄기세포추출물은 nuclear factor kappa light chain enhancer of activated B cells, inducible nitric oxide synthase 및 interleukin-6와 같은 염증 관련 단백질 발현을 감소시켜 H2O2로 유도된 염증반응 보호 효과를 확인할 수 있었다. 뿐만 아니라, 무막줄기세포추출물 처리 군은 caspase-9, -3, poly (ADP-ribose) polymerase 단백질 발현 감소와 B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 비율을 저하시켜 H₂O₂ 유도 산화적 손상에 대한 세포사멸 보호 효과를 보였다. 따라서 지방조직 유래 무막줄기세포추출물은 H2O2 유도 산화적 손상에 대한 HPLF 세포의 염증반응 및 세포사멸을 저해함으로써 치주염으로부터 보호 효과가 있어, 치주질환 치료용 소재로써의 활용 가능성이 있을 것으로 기대된다. Periodontal inflammation, a major kind of periodontal diseases, is characterized to bleed, pain, and teeth loss, and it is resulted from oxidative stress. Membrane-free stem cell extract could avoid the immunogencity rejection by removal of cell membrane. In the present study, we investigated the protective effect of membrane-free stem cell extract from oxidative stress-induced periodontal inflammation in human periodontal ligament fibroblasts (HPLF). In the cell viability measurement, membrane-free stem cell extract showed significant increase of cell viability, compared with the H₂O₂-treated control group. To further investigation of molecular mechanisms, we measured inflammation and apoptosis related protein expressions. Membrane-free stem cell extract attenuated inflammation-related protein expressions such as nuclear factor kappa light chain enhancer of activated B cells, inducible nitric oxide synthase, and interleukin-6. In addition, the treatment of membrane-free stem cell extract decreased apoptotic protein expressions such as cleaved caspase-9, -3, poly (ADP-ribose) polymerase, and B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 ratio in the H₂O₂-treated HPLF cells. In conclusion, membrane-free stem cell extract exhibited anti-oxidative stress effects by regulation of inflammation and apoptosis in HPLF, suggesting that it could be used as the treatment agents for periodontal inflammatory disease.
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Protective effect of Cordyceps militaris against hydrogen peroxide-induced oxidative stress in vitro
He, Mei Tong,Lee, Ah Young,Park, Chan Hum,Cho, Eun Ju The Korean Nutrition Society 2019 Nutrition Research and Practice Vol.13 No.4
BACKGROUND/OBJECTIVES: Excessive production of reactive oxygen species (ROS) such as hydroxyl (${\cdot}OH$), nitric oxide (NO), and hydrogen peroxide ($H_2O_2$) is reported to induce oxidative stress. ROS generated by oxidative stress can potentially damage glial cells in the nervous system. Cordyceps militaris (CM), a kind of natural herb widely found in East Asia. In this study, we investigated the free radical scavenging activity of the CM extract and its neuroprotective effects in $H_2O_2$-induced C6 glial cells. MATERIALS/METHODS: The ethanol extract of CM ($100-1,000{\mu}g/mL$) was used to measure DPPH, ${\cdot}OH$, and NO radical scavenging activities. In addition, hydrogen peroxide ($H_2O_2$)-induced C6 glial cells were treated with CM at $0.5-2.5{\mu}g/mL$ for measurement of cell viability, ROS production, and protein expression resulting from oxidative stress. RESULTS: The CM extract showed high scavenging activities against DPPH, ${\cdot}OH$, and NO radicals at concentration of $1,000{\mu}g/mL$. Treatment of CM with $H_2O_2$-induced oxidative stress in C6 glial cells significantly increased cell viability, and decreased ROS production. Cyclooxygenase-2 and inducible nitric oxide synthase protein expression was down-regulated in CM-treated groups. In addition, the protein expression level of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK), phospho-c-Jun N-terminal kinase (p-JNK), and phospho-extracellular regulated protein kinases (p-ERK) in $H_2O_2$-induced C6 glial cells was down-regulated upon CM administration. CONCLUSION: CM exhibited radical scavenging activity and protective effect against $H_2O_2$ as indicated by the increased cell viability, decreased ROS production, down-regulation of inflammation-related proteins as well as p-p38, p-JNK, and p-ERK protein levels. Therefore, we suggest that CM could play the protective role from oxidative stress in glial cells.
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Preparation and High Degradation Activity of Supported Nano-Bi2WO6-TiO2/Nickel Foam Photocatalyst
Qin Tong,Ya-Mei Dong,Xiao-Jia Wang,Peng Yan,Dan-Nong He 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6
The supported nano-Bi2WO6–TiO2/nickel foam photocatalyst was synthesized via spraying method with silica sol as a binder. The as-prepared photocatalyst with large surface and thermal stability exhibited enhanced photocatalytic performance for degradation of Rhodamine B (RhB) solution in sunlight irradiation. The results showed that the RhB solution could be degraded to 71.6% within 60 min, exhibiting that improved photocatalytic activity increased by 77.6%, compared with that of pure Bi2WO6–TiO2 nanoparticles. Its high photocatalytic activity was due to the presence of nickel foam in the catalytic process, making the Bi2WO6–TiO2 highly dispersed and increasing the contact area of the photocatalysts with the organic pollutants. At the same time, a possible photocatalytic mechanism for the enhanced photocatalytic performance was investigated.
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