SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion

An Sunho,Vo Tam Thuy Lu,Son Taekwon,Choi Hoon,Kim Jinyoung,Lee Juyeon,Kim Byung Hoon,Choe Misun,Ha Eunyoung,Surh Young-Joon,Kim Kyu-Won,Seo Ji Hae 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Human sterile α motif and HD domain-containing protein 1 (SAMHD1) has deoxyribonucleoside triphosphohydrolase (dNTPase) activity that allows it to defend against human immunodeficiency virus type I (HIV-1) infections and regulate the cell cycle. Although SAMHD1 mutations have been identified in various cancer types, their role in cancer is unclear. Here, we aimed to investigate the oncogenic role of SAMHD1 in human clear cell renal cell carcinoma (ccRCC), particularly as a core molecule promoting cancer cell migration. We found that SAMHD1 participated in endocytosis and lamellipodia formation. Mechanistically, SAMHD1 contributed to the formation of the endosomal complex by binding to cortactin. Thereafter, SAMHD1-stimulated endosomal focal adhesion kinase (FAK) signaling activated Rac1, which promoted lamellipodia formation on the plasma membrane and enhanced the motility of ccRCC cells. Finally, we observed a strong correlation between SAMHD1 expression and the activation of FAK and cortactin in tumor tissues obtained from patients with ccRCC. In brief, these findings reveal that SAMHD1 is an oncogene that plays a pivotal role in ccRCC cell migration through the endosomal FAK-Rac1 signaling pathway.

Development of a genome editing tool for Pseudomonas putida

Sunho CHOI,Daegeun CHA,SungKuk LEE 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.10

Effects of mirror polishing brick surface on process yield of multi-wire slicing for thin single-crystalline silicon solar cells

Choi, Sunho,Jang, Boyun,Kim, Joonsoo,Song, Heeun,Baek, Taehyeon,Han, Moonhee Elsevier 2015 SOLAR ENERGY -PHOENIX ARIZONA THEN NEW YORK- Vol.122 No.-

<P><B>Abstract</B></P> <P>Thin silicon (Si) wafers with thickness of 100–140μm were obtained by using a multi-wire slicing process with a SiC slurry. We investigated the process yields of wafer slicing as well as physical/electrical properties of the sliced wafers with various thicknesses. As the wafer thickness decreases, the process yield abruptly decreased due to wafer breakage during the slicing process, and conventional polishing the brick surface was not enough to gain a considerable process yield. However, elimination of defects on the brick surface by mirror polishing resulted in an 83.1% enhancement of yield even for wafers with a thickness of 100μm. The number of wafers obtained was even higher than that of conventional 180μm-thick wafers (479 vs. 415 wafers in this research). Investigation of the microstructure of brick surfaces revealed that surface defects on bricks were main parameter to determine the yield of slicing process. Surface defects containing the micro-cracks introduced residual stress, which decreased the slicing process yield especially for thinner wafers. From measurements of physical and electrical properties, it was revealed that the relative total thickness variations (TTVs) and bowings increased and the characteristic fracture strength of sliced wafer and conversion efficiencies decreased as the wafer thickness decreased. For the wafer with thickness of 100μm, the relative TTV and bowing were 14.1% and 22.5mm, respectively. The conversion efficiency of a solar cell using this wafer was 17.6%, while that of a conventional Si solar cell using a 180μm-thick wafer was 18.4%.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Thin silicon wafers with thickness of 100–140μm were sliced by multi-wire slicing. </LI> <LI> Physical and electrical properties of thin Si wafers were investigated. </LI> <LI> Subsurface defects of the brick surface critically determined process yield in slicing Si wafers. </LI> </UL> </P>

Cereblon Maintains Synaptic and Cognitive Function by Regulating BK Channel

Choi, Tae-Yong,Lee, Seung-Hyun,Kim, Yoon-Jung,Bae, Jae Ryul,Lee, Kwang Min,Jo, Youhwa,Kim, Soo-Jeong,Lee, A-Ram,Choi, Sekyu,Choi, La-Mee,Bang, Sunhoe,Song, Mi-Ryoung,Chung, Jongkyeong,Lee, Kyung Jin,K The Society 2018 The Journal of neuroscience Vol.38 No.14

<P>Mutations in the cereblon (CRBN) gene cause human intellectual disability, one of the most common cognitive disorders. However, the molecular mechanisms of CRBN-related intellectual disability remain poorly understood. We investigated the role of CRBN in synaptic function and animal behavior using male mouse and Drosophila models. Crbn knock-out (KO) mice showed normal brain and spine morphology as well as intact synaptic plasticity; however, they also exhibited decreases in synaptic transmission and presynaptic release probability exclusively in excitatory synapses. Presynaptic function was impaired not only by loss of CRBN expression, but also by expression of pathogenic CRBN mutants (human R419X mutant and Drosophila G552X mutant). We found that the BK channel blockers paxilline and iberiotoxin reversed this decrease in presynaptic release probability in Crbn KO mice. In addition, paxilline treatment also restored normal cognitive behavior in CrbnKOmice. These results strongly suggest that increased BK channel activity is the pathological mechanism of intellectual disability in CRBN mutations.</P>

Construction and enhancement of E. coli Biosensor for detection of landmines

Sunho CHOI,Hyunji RHO,Sungkuk LEE 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

공통 데이터 모델(CDM)을 위한 비정형 · 반정형 의료문서 데이터의 정형화

최선호(Sunho Choi),김유중(Yoojoong Kim),석준희(Junhee Seok) 한국정보과학회 2019 한국정보과학회 학술발표논문집 Vol.2019 No.12

초음파를 활용한 3D 프린터 제작품 상태 평가 연구

최선호(Sunho Choi),이재선(Jaesun Lee),박준필(Junpil Park) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.05

With the development of 3D printing technology, various products are produced and used in industrial sites. While 3D printers have the advantage of complex shape production, there are still many points to be improved in terms of strength and quality. The nondestructively material properties measurements technology using ultrasonic waves has been applied in various ways to metallic and non-metallic materials. However, studies conducted on 3D printing products are insufficient, and studies on changes in material properties according to additive manufacturing conditions are also relatively insufficient. In this study, the state of the material according to the additive manufacturing conditions was measured in a non-destructive method using ultrasonic waves, and the correlation between the state of the material and the ultrasonic properties was studied. It was confirmed that the characteristics of ultrasonic waves change according to the lamination conditions, and based on these results, it is expected that the state and reliability of 3D printing products can be secured and applied to industrial sites.

C 대역 데이터 링크 적용을 위한 새로운 형태의 급전기 안테나 설계

최선호(Sunho Choi),정선우(Sun-woo Jeong),안재영(Jae-young An),조봉현(Bong-hyun Cho),박익서(Ick-suh Park) 항공우주시스템공학회 2018 항공우주시스템공학회 학술대회 발표집 Vol.2018 No.11

MWCNT 보강된 에폭시 수지의 장기성능 예측

최순호(Sunho Choi),차재호(Jaeho Cha),윤성호(Sungho Yoon) 한국추진공학회 2018 한국추진공학회 학술대회논문집 Vol.2018 No.12

‘홍익인간’은 무엇을 뜻하나?

최선호(Sunho Choi) 세계환단학회 2021 세계환단학회지 Vol.8 No.2

오늘날에는 홍익인간이라는 개념을 서구적이고 현대적인 사유체계 속에서 해석함에 따라, 홍익인간의 의미가 크게 왜곡되어 있다. 본 논문에서는 홍익인간이라는 개념이 만들어졌던 당시 우리의 고유한 사유체계를 통해 홍익인간의 개념을 해석해보았으며, 이에 따라 홍익인간의 올바른 의미가 ‘도道로써 널리 또 크게 중衆을 깨우쳐서, 사람과 사람 사이 삶의 도리를 실천하며 살아가게 하다, 또는 그렇게 살아가는 세상’으로 해석하고 있다. 이는 ‘깨우침’과 ‘공존 상생’을 강조하는 개념으로, 오늘날 우리가 일반적으로 이해하고 있는 홍익인간의 의미와는 크게 다름을 보여주고 있다, 즉 홍익인간이 ‘이타적인 사람의 모습’을 강조한, ‘널리 사람 또는 세상을 이롭게 하다’라는 의미가 아님을 강조하고 있다. 본 논문에서는 ‘고문헌에 기록된 한문 해석에 대한 접근법’과 ‘기존 역사연구방법에 대한 비판’을 통해, 홍익인간의 의미를 원래대로 복원하여 제대로 해석하기 위한 방법론을 제시하고 있다. 또한 ‘홍익弘益’은 ‘홍도익중弘道益衆’에서, ‘인간人間’은 ‘인재기간人在其間’에서 온 표현임을 보여주고 있다. 이를 통해 우리는 홍익인간이라는 개념이 삶에 대한 깊은 통찰력과 원대한 이상에서 나온 것임을 알 수 있다. In modern days, the Korean traditional concept of 'Hong-Ik-In-Gan' has been grossly misinterpreted and misunderstood, mainly due to the Westernized, modern way of life and thinking. In this article, we use the Korea's own traditional philosophy which dates back to approximately 9,000 years ago to reinterpret and restore the original concept of 'Hong-Ik-In-Gan'. The Korea's own traditional philosophy is unique and different from other Asian philosophies such as Confucianism, Taoism, Buddhism, etc. The origin of the Korea's own traditional philosophy can be traced back to two ancient Korean writings, Cheonbugyeong and Samilsingo, which have been passed on to today's Koreans for over 9,000 years. Cheonbugyeong and Samilsingo are very short, consisting of only 81 and 366 ideographic characters (known as Chinese characters today), respectively, but contain limitless implications to our lives and the universe well beyond imagination. The backbone of Korea's traditional philosophy laid down by Cheonbugyeong and Samilsingo has been further explained in detail in five old Korean books such as Samseong-gi I, Samseong-gi II, Dangun-Segi, Bukbuyeo-gi, and Taebaek-Ilsa, and these five old Korean books are put together in one book, known as Hwandan Gogi. According to the Korea's traditional philosophy, the meaning of 'Hong-Ik-In-Gan' is 'Enlighten (and/or brighten and/or civilize) the people by having them understand Do (道) so that they can live in accordance with Do(道), i.e., live together in mutual understanding and mutual prosperity‘. The meaning of Do(道) in the context of Korea's traditional philosophy is also different from other Asian philosophies in that it is very much scientific and practical. In such context, the concept of 'Hong-Ik-In-Gan' implies that no one can exist/live alone and that everyone needs everyone else. 'Hong-Ik-In-Gan' emphasizes 1) people should be enlightened (and/or brightened and/or civilized), and 2) they can only exist and should live together with mutual understanding and mutural prosperity. Unfortunately, the meaning of 'Hong-Ik-In-Gan' has been misinterpreted and misunderstood in modern days as one meaning 'Benefit and/or contribute to the wellbeing of other people so that all human beings can live together in peace and harmony'. That is, 'Hong-Ik-In-Gan' is misunderstood as a concept that emphasizes being altruistic as opposed to being selfish, which is the outcome of wrongly interpreting the unique Korean philosophical concept in a non-Korean philosophical context. Though most people praise being altruistic is more ethical and better to a society than being selfish, being altruistic is as troublesome as being selfish in that it is an unstable and incomplete concept as well. In this article, we also propose new methodology for interpreting the meaning of the ancient Korean writings in a more adequate way. We also show the origin of the expression, 'Hong-Ik-In-Gan'. In sum, we can say that the concept of 'Hong-Ik-In-Gan' truly reveals the deep insight and understanding about our life and the grandiose vision to our future.