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Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.
단상 멀티레벨 능동전력필터를 위한 고조파 검지 기법 비교
김윤호(Yoon-Ho Kim),김수홍(Soo-Hong Kim),김성민(Sung-Min Kim),서강문(Kang-Moon Seo) 전력전자학회 2005 전력전자학술대회 논문집 Vol.- No.-
In this paper, harmonic detecting methods for the active power application are investigated. They are RDFT, Kalman Filter, Adaptive predictive filter, Instantaneous reactive power detecting method, Improved adaptive filter detecting method. The 5 harmonic detecting methods are simulated and their characteristics for the active filter application are compared using simulation results.
저서성 (底棲性) 대형무척추동물에 (大型無脊椎動物) 의한 금호강 (琴湖江) 수계의 (水系) 생물학적 수질평가
윤일병,공동수,이상협 ( Il Byong Yoon,Dong Soo Kong,Sang Hyeop Lee ) 한국하천호수학회 1992 생태와 환경 Vol.25 No.3
Keumho river is flowing across Taeku city, which is α-mesosaprobic according to annual and situal average level. Sillyong stream inflowing to upper branch is β-mesosaprobic, and Omok stream is α-mesosaprobic, and Namchon, Pulno, and Palkye stream were polysaprobic, respectively. Then, these streams impact on main branch. But Tonghwa stream is a little clean and dilutes a very polluted middle main branch. Among them, Namchon, Pulno, Pakye, and lower main branch are polysaprobic zones with McNaughton`s dominance DI above 0.8, Kno¨pp`s B.E.O.L. above 90, Pantle-Buck`s S above 3.2, Beck-Tsuda`s BI below 10, Shannon`s diversity below 1.5, Yoon-Kong`s TBS below 30. Therefore these zones seem to be target area to be ameliorated in Keumho basin. In the fluctuation of biotic indices, B.E.O.L., Saprobic index S, SI, dominance DI in last season of 1990 were lower than those in same period of 1991, but diversity H`, BI, and TBS were higher.
Soo-Yoon Sung(Soo-Yoon Sung),Jin Ho Song(Jin Ho Song),Byoung Hyuck Kim(Byoung Hyuck Kim),Yoo-Kang Kwak(Yoo-Kang Kwak),Kyung Su Kim(Kyung Su Kim),Gyu Sang Yoo(Gyu Sang Yoo),Hwa Kyung Byun(Hwa Kyung Byu 대한방사선종양학회 2024 Radiation Oncology Journal Vol.42 No.1
Advances in radiotherapy (RT) techniques, including intensity-modulated RT and image-guided RT, have allowed hypofractionation, increasing the fraction size over the conventional dose of 1.8–2.0 Gy. Hypofractionation offers advantages such as shorter treatment times, improved compliance, and under specific conditions, particularly in tumors with a low α/β ratio, higher efficacy. It was initially explored for use in RT for prostate cancer and adjuvant RT for breast cancer, and its application has been extended to various other malignancies. Hypofractionated RT (HFRT) may also be effective in patients who are unable to undergo conventional treatment owing to poor performance status, comorbidities, or old age. The treatment of brain tumors with HFRT is relatively common because brain stereotactic radiosurgery has been performed for over two decades. However, re-irradiation of recurrent lesions and treatment of elderly or frail patients are areas under investigation. HFRT for head and neck cancer has not been widely used because of concerns regarding late toxicity. Thus, we aimed to provide a comprehensive summary of the current evidence for HFRT for brain tumors and head and neck cancer and to offer practical recommendations to clinicians faced with the challenge of choosing new treatment options.
Assessment of Fracture Behaviors for CIP Anchors Fastened to Cracked and Uncracked Concretes
Yoon, Young-Soo,Kim, Ho-Seop,Kim, Sang-Yun Korea Concrete Institute 2001 KCI concrete journal Vol.13 No.2
This paper presents the crack effect on CIP anchors and prediction of tensile capacity, as governed by concrete cone failure. Single anchors where located at center of concrete specimen. Three different types of cracks such as crack width of 0.2 mm and 0.5 mm, crack depth of 10 cm and 20cm , and crack location of center and off-center point were simulated. Static tensile load was applied to 7/8-in. CIP anchors of 10 cm and 20 cm embedment length in concrete with compressive strength of 280 kgf/$\textrm{cm}^2$. Tested pullout capacities were compared to the values determined using current design methods (such as ACI 349-97, ACI 349 revision and CEB-FIP which is based on CCD Method). The comparison of CCD Method and ACI revision showed almost the same values in uncracked concrete specimen. In cracked concrete, CCD Method predicted conservative values. Three-dimensional non-linear FEM modeling also has been performed to determine the stresses distribution and crack inclination.
( Sung Eun Lee ),( Byeoung Soo Park ),( Jeong Jun Yoon ) 한국균학회 2010 Mycobiology Vol.38 No.4
Formic acid is a representative carboxylic acid that inhibits bacterial cell growth, and thus it is generally considered to constitute an obstacle to the reuse of renewable biomass. In this study, Saccharomyces cerevisiae was used to elucidate changes in protein levels in response to formic acid. Fifty-seven differentially expressed proteins in response to formic acid toxicity in S. cerevisiae were identified by 1D-PAGE and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) analyses. Among the 28 proteins increased in expression, four were involved in the MAP kinase signal transduction pathway and one in the oxidative stress-induced pathway. A dramatic increase was observed in the number of ion transporters related to maintenance of acid-base balance. Regarding the 29 proteins decreased in expression, they were found to participate in transcription during cell division. Heat shock protein 70, glutathione reductase, and cytochrome c oxidase were measured by LC-MS/MS analysis. Taken together, the inhibitory action of formic acid on S. cerevisiae cells might disrupt the acidbase balance across the cell membrane and generate oxidative stress, leading to repressed cell division and death. S. cerevisiae also induced expression of ion transporters, which may be required to maintain the acid-base balance when yeast cells are exposed to high concentrations of formic acid in growth medium.
Enhancement of Gene Delivery Using Novel Homodimeric Tat Peptide Formed by Disulfide Bond
( Soo Jin Lee ),( Sung Hwa Yoon ),( Kyung Oh Doh ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.8
Cationic liposomes have been actively used as gene delivery vehicle because of their minimal toxicity, but their relatively low efficiency of gene delivery is the major disadvantage of these vectors. Recently, cysteine residue incorporation to HIV-1 Tat peptide increased liposome mediated transfection compared with unmodified Tat peptide. Therefore, we designed a novel modified Tat peptide having a homodimeric (Tat-CTHD, Tat-NTHD) and closed structure (cyclic Tat) simply by using the disulfide bond between cysteines to develop a more efficient and safe nonviral gene delivery system. The mixing of Tat-CTHD and Tat-NTHD with DNA before mixing with lipofectamine increased the transfection efficiency compared with unmodified Tat peptide and lipofectamine only in MCF-7 breast cancer cells and rat vascular smooth muscle cells. However, cyclic Tat did not show any improvement in the transfection efficiency. In the gel retardation assay, Tat-CTHD and Tat-NTHD showed more strong binding with DNA than unmodified Tat and cyclic Tat peptide. This enhancement was only shown when Tat-CTHD and Tat-NTHD were mixed with DNA before mixing with lipofectamine. The effects of Tat- CTHD and Tat-NTHD were also valid in the experiment using DOTAP and DMRIE instead of lipofectamine. We could not find any significant cytotoxicity in the working concentration and more usage of these peptides. In conclusion, we have designed a novel transfection-enhancing peptide by easy homodimerization of Tat peptide, and the simple mix of these novel peptides with DNA increased the gene transfer of cationic lipids more efficiently with no additional cytotoxicity.