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Hypoallergenic and Physicochemical Properties of the A2 β-Casein Fraction of Goat Milk
Tae-Hwan Jung,Hyo-Jeong Hwang,Sung-Seob Yun,Won-Jae Lee,Jin-Wook Kim,Ji-Yun Ahn,Woo-Min Jeon,Kyoung-Sik Han2.6* 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.6
Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 β-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 β-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at 25°C). Selective reduction of β- lactoglobulin and αs-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 β-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 β-casein fraction. There was no significant difference in levels of both indicators between A2 β-casein treatment and the control (no protein treatment). The A2 β-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 β-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 β-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.
Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
Identification of genomic regions associated with white fruitbody forming in Flammulina velutipes
Sung-I Woo,Jae-Gu Han,Pyung-Gyun Shin,Youn-Lee Oh,Song-Hee Lee,Kyung-Soo Kim,Sung-Hwan Jo,Won-Sik Kong 한국버섯학회 2014 버섯 Vol.18 No.2
A total of 25 strains of Flammulina velutipes were analyzed to identify the genomic regions responsible for producing white-fruiting body. NGS data was yielded by using Illumina Hiseq platform. Short reads were filtered by quality score and read length were mapped on the reference genome (KACC42780). Between the white- and brown fruitbody forming strains, we found 9376 SNPs, of which 8178 were non-polymorphic and 1198 were polymorphic. There is a high possibility that SNPs can be detected among the white strains as homozygous because white phenotype is recessive in F. velutipes. Thus, we constructed SNP matrix within 8 white strains. SNPs discovered between mono3 and mono19, the parental monokaryotic strains of 4120 strain (white), were excluded from the candidate. If the genotypes of SNPs detected between white and brown strains were identical with those in mono3 and mono19 strains, they were included in candidate as a priority. As a result, if more than 5 candidates SNPs were localized in single gene, we regarded as they are possibly related to the white color. In F. velutipes genome, chr08: 950kb-2650kb, chr09: 500kb-1400kb, chr09: 2800kb-4350kb, and chr11: 2450kb-3500kb regions were identified to be associated with white fruitbody forming.
Han, Paul,Jang, Young Woong,Kim, Jung Sung,Yoo, Oui Sik,Lee, Myung Chul,Lim, Dohyung Korean Society for Precision Engineering 2014 International Journal of Precision Engineering and Vol.15 No.12
This study aimed to determine the clinical applicability of the new TKA by evaluating biomechanical stability through measurement of contact pressure and strain distribution in the proximal tibia during the stand-sit-stand motion. The reaction forces generated during knee flexion from <TEX>$0^{\circ}$</TEX> to <TEX>$140^{\circ}$</TEX> were measured in eight men (<TEX>$25{\pm}2years$</TEX>, <TEX>$23{\pm}4kg/m^2$</TEX>) using two force-plates integrated with a computer-aided video motion capture system and were used for load applications in the mechanical test to identify the contact pressure and strain distribution in the tibia with the new TKA. The results indicate that the new TKA may be appropriate for sharing and transfer of physiological loads on the tibia without critical damage to the tibia and TKA during the high deep flexion associated with the stand-sit-stand motion.
Strategically Designed Zeolitic Imidazolate Frameworks for Controlling the Degree of Graphitization
Han, Sang A,Lee, Jaewoo,Shim, Kyubin,Lin, Jianjian,Shahabuddin, Mohammed,Lee, Jong-Won,Kim, Sang-Woo,Park, Min-Sik,Kim, Jung Ho The Chemical Society of Japan 2018 Bulletin of the Chemical Society of Japan Vol.91 No.10
Tumorigenicity Evaluation of Umbilical Cord Blood-derived Mesenchymal Stem Cells
Sang-Jin Park,Hyun-Jung Kim,Woojin Kim,Ok-Sun Kim,Sunyeong Lee,Su-Yeon Han,Eun Ju Jeong,Hyun-shin Park,Hea-Won Kim,Kyoung-Sik Moon 한국독성학회 2016 Toxicological Research Vol.32 No.3
Mesenchymal stem cells (MSCs) have been identified in multiple types of tissue and exhibit characteristic self-renewal and multi-lineage differentiation abilities. However, the possibility of oncogenic transformation after transplantation is concerning. In this study, we investigated the tumorigenic potential of umbilical cord blood-derived MSCs (hUCB-MSCs) relative to MRC-5 and HeLa cells (negative and positive controls, respectively) both in vitro and in vivo. To evaluate tumorigenicity in vitro, anchorage-independent growth was assessed using the soft agar colony formation assay. hUCB-MSCs and MRC-5 cells formed few colonies, while HeLa cells formed a greater number of larger colonies, indicating that hUCBMSCs and MRC-5 cells do not have anchorage-independent proliferation potential. To detect tumorigenicity in vivo, hUCB-MSCs were implanted as a single subcutaneous injection into BALB/c-nu mice. No tumor formation was observed in mice transplanted with hUCB-MSCs or MRC-5 cells based on macroand microscopic examinations; however, all mice transplanted with HeLa cells developed tumors that stained positive for a human gene according to immunohistochemical analysis. In conclusion, hUCBMSCs do not exhibit tumorigenic potential based on in vitro and in vivo assays under our experimental conditions, providing further evidence of their safety for clinical applications.
Topoisomerase-II-Inhibitory Principles from the Stems of Spatholobus suberectus
Han, Ah-Reum,Park, Hyen ,Joo,Chen, Daofeng,Jang, Dae ,Sik,Kim, Hwa-Jung,Lee, Sang ,Kook,Seo, Eun-Kyoung VERLAG HELVETICA CHIMICA ACTA AG 2007 Chemistry & Biodiversity Vol. No.
<P>Bioassay-guided fractionation of the stems of Spatholobus suberectus led to the isolation of procyanidin B4 (=(+)-catechin-(4→8)-(−)-epicatechin) (1) and (+)-catechin-(4→8)-(+)-catechin-(4→8)-(−)-epicatechin (2). These compounds, isolated before from other sources, were found to be highly potent inhibitors of DNA-topoisomerase-II (Topo-II)-mediated KDNA decatenation, with IC<SUB>50</SUB> values of 22.5±2.3 and 21.9±2.2 nM, resp.</P>