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Song, Sun U.,Cha, Young-Deog,Han, Jeoung-Uk,Oh, In-Suk,Choi, Kyoung Baek,Yi, Youngsuk,Hyun, Jong-Pil,Lee, Hyeon-Youl,Chi, Guang Fan,Lim, Chae-Lyul,Ganjei, J. Kelly,Noh, Moon-Jong,Kim, Seong-Jin,Lee, D Mary Ann Liebert, Inc 2005 Tissue engineering Vol.11 No.9-10
<P>The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-beta1 (TGF-beta1)-producing human chondrocytes (hChon-TGF-beta1) and primary human chondrocytes (hChon) ('mixed cells'), compared with either hChon-TGF-beta1 or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into the backs of immune-deficient nude mice to test the feasibility of cartilage formation in vivo. Both the mixed cells and the hChon-TGF-beta1 cells alone induced cartilage formation in nude mice, whereas hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with hChon-TGF-beta1 cells with or without mixing additional untransduced hChon cells, and hyaline cartilage regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and the quality of tissue generated after implanting were evaluated on the basis of a histological grading system modified from O'Driscoll et al. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed cells (14.2 +/- 0.9) produced significantly better results than hChon-TGF-beta1 (9.0 +/- 1.7) or hChon (8.0 +/- 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues produced after treatment with either mixed cells or hChon-TGF-beta1 cells alone showed hyaline cartilage- like characteristics. These results suggest that the implantation of mixed cells may be a clinically efficient method of regenerating hyaline articular cartilage.</P>
Clostridium속 세균포자의 안정성과 Plasmids 탐색
윤기홍,이정기,김병홍,소선주,전영숙,권기석,Z.G.Zeikus 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.2
제1, 2차세계대전 동안 분리되어 soil culture로 보존되어 온 131주의 Clostridium속 세균 중에서 100주가 재생되어 76%의 높은 재생력을 보여주었다. 이들 재생균주들의 발효산물을 분석한 결과, 주요산물이 acetone, ethanol 및 butanol인 solvent 생산균들은 63주이고 acetic acid와 butyric acid를 생성하는 산 생성균은 37주였다. 재생된 100주 중 plasmid를 갖는 strain은 50주로 plasmid 함유율은 50%였다. 균주에 따라서 하나에서 여러 개의 plasmids를 가지고 있었으며 그 크기는 1.0kb에서 50kb에 달하였다. Stock cultures of Clostridium, which had been maintained as spores with soil as carrier for 50-70 years, were used to determine their revival ratio, and plasmids were screened among the revived strains. From 131 soil cultures, 100 strains grew in the liguid medium giving the revival ratio of 76%. Analyses of the fermentation products divided the revived strains into 63 solventogenic strains and 37 acidogenic strains. Agarose gel electrophoresis showed one or more plasmid bands in the cleared lysates of 50 revived cultures. The sizes of the plasmids range in size from 1 to more than 50 kilobase pairs.
MutMap 분석에 의한 벼 왜성 돌연변이 계통의 변이 유전자 탐색
오준(Jun Oh),천경성(Kyeong-Seong Cheon),강도유(Do-Yu Kang),김송림(Song Lim Kim),이은경(Eungyeong Lee),김년희(Nyunhee Kim),오효자(Hyoja Oh),최인찬(Inchan Choi),백정호(Jeongho Baek),윤인선(In Sun Yoon),김경환(Kyung-Hwan Kim),정남진(Nam-J 한국육종학회 2020 한국육종학회지 Vol.52 No.1
A dwarf mutant rice line was selected from an Ac/Ds insertion mutant population and named dwf1. The phenotype of F1 and F2 plants derived from a cross between dwf1 and Dongjin indicated that a single recessive gene is responsible for the mutant phenotype, and we named this gene dwf1. Resequencing of the dwf1 line and Dongjin (wild type) revealed 42,386 homozygous single nucleotide polymorphisms (SNPs) between dwf1 line and Dongjin. MutMap analysis was performed by sequencing a DNA pool prepared from 100 mutant type plants in the dwf1/Dongjin F2 population, and it was found that the dwf1 gene was located in the 23 ~ 30 Mbp region on chromosome 4. In this region, we found a non-synonymous SNP in the Os04g0469800 gene, which was reported as D11 gene encoding a cytochrome P450 family protein involved in the biosynthesis of brassinosteroids (BRs). This SNP was regarded as the causative SNP for the dwf1 phenotype, and the dwf1 gene is a novel allele of D11. We performed mapping of the dwf1 gene with five SNP markers on chromosome 4 with 190 dwf1/Dongjin F2 plants. The phenotype of F2 plants was completely co-segregated with genotypes of the J10402 marker, which was developed based on the non-synonymous SNP in the D11 gene. These results will contribute to the study of the molecular biological functions of the D11 gene and BRs.
Efficacy and Toxicity of Pemetrexed as a Third-line Treatment for Non-small Cell Lung Cancer
Sun, J.-M.,Lee, K.-W.,Kim, J. H.,Kim, Y. J.,Yoon, H. I.,Lee, J.-H.,Lee, C.-T.,Lee, J. S. Oxford University Press 2009 Japanese journal of clinical oncology Vol.39 No.1
<P>OBJECTIVE: Pemetrexed has been approved for second-line treatment in non-small cell lung cancer (NSCLC). However, the role of third-line pemetrexed therapy in NSCLC has not yet been generally accepted. We attempted to validate third-line pemetrexed therapy and evaluate predictive factors for pemetrexed therapy for NSCLC. METHODS: Medical records of NSCLC patients who received pemetrexed therapy that progressed after systemic therapy were reviewed retrospectively. We stratified patients according to clinicopathologic characteristics to find predictive factors for pemetrexed therapy. RESULTS: A total of 100 patients were eligible for analysis, and overall progression-free survival (PFS) was 3.03 months. The objective response rate was 12%, and the toxicity profile was favorable. Pemetrexed was used as a second-line treatment in 30% of patients, and as third- or further-line treatment in 70%. Comparing the efficacy of pemetrexed in these two settings (second-line versus third- or further-line), there was no significant difference in terms of PFS (3.07 versus 2.83 months, P = 0.86). When we evaluated predictive factors by multivariate analysis, performance status significantly influenced PFS. CONCLUSIONS: Pemetrexed is a suitable third-line treatment option with good efficacy and tolerable toxicity profile for NSCLC.</P>
A Review of the Jindo, Korean Native Dog - Review -
Lee, C.G.,Lee, J.I.,Lee, C.Y.,Sun, S.S. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.3
The Jindo is a Korean native dog, well-known for its hunting and guarding abilities. When he gives his devotion to one individual, he gives it whole-heartedly. He is not tempted easily and impetuous. The breed was not developed. but the dog retained their original qualities -loyal, alert, fearless, obedient, watchful, intelligent, energetic- to survive in the harsh environment of the Jindo island. The dog had been spread over the entire Korean peninsula from the time unknown, and the ones in the Jindo island, isolated until lately, survived and maintained their original characteristics. They are now spread over the entire Jindo County consisted of many islands, whence the breed name came. The Jindo comes in a variety of colors and color combinations, with the fawn and white colorings predominant. The dog is one of the Korean natural monuments, protected by law since early 1960s. The Jindo gained official approval by the Federation Cynologique Internationale as a hunting dog. Apart from the basic housetraining, the dog rarely gets training. Many people have attempted to preserve its pure bloodlines and original qualities. Today, there are a total of 10,356 Jindoes being raised over the entire Jindo County, and many more are kept elsewhere. A research into genetic characteristics of the Jindo is now going on, using the technique of isozyme electrophoresis. The Jindo Dog Breeding Management Center has been reinforced lately, and in addition to their routines, the Center is to work on the breeding of the Jindo. Efforts should be made in the future to produce stable, trustworthy Jindoes according to their proposed use and to modify their temperament in order to make it more widely acceptable as a pet and companion dog in the strangers home.