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- 저자 : SoonOk Woo (검색결과 32 건)
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In vitro Skin Irritation Test of Honeypolis using Human Skin Model
Woo, SoonOk,Sangmi Han,Inpyo Hong,Kim, Sung-kuk 한국양봉학회 2018 韓國養蜂學會誌 Vol.33 No.4
Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on EpiDerm<SUP>TM</SUP>, a reconstituted threedimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study under the given conditions honeypolis showed no irritant effects. Honeypolis meets acceptance criteria if: mean absolute OD 570 nm of the three negative control tissues is ≥0.8 and ≤2.8, mean relative tissue viability of the three positive control tissues is ≤ 20%, standard deviation of relative tissue viability obtained from each three concurrently tested tissues is ≤ 18%. Honeypolis is therefore classified as “non-irritant” in accordance with UN GHS “No Category”.
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Decolorization of Triphenylmethane Dyes by Wild Mushrooms
Kang, Hyeon Woo,Yang, Yun Hui,Kim, Sang Woo,Kim, Soonok,Ro, Hyeon-Su 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3
Triphenylmethane dyes such as Crystal Violet (CV) and Malachite Green (MG) are common textile dyes. MG, which is toxic to humans, is widely used in aquaculture as an antifungal agent. In this study, 56 mushroom strains from 12 species of wild mushrooms were examined on dye-containing PDA plates to evaluate their potential for the bioremediation of synthetic dyes. Pycnoporus coccineus, Coriolus versicolor, and Lentinula edodes showed fair growth on CV, but only a few survived on MG. However, a decolorization experiment in an aqueous system revealed that the growth on MG-containing solid medium did not directly match the decolorization of MG in the aqueous system. C. versicolor IUM0061 grew well on both MG and CV plates, but could not decolorize MG in the reaction mixture. Conversely, HPLC analysis revealed that P. coccineus IUM0032, which could not grow on the MG plate, completely mineralized MG within 3 days. A subsequent enzyme activity assay revealed a high lignin peroxidase activity in the reaction mixture, indicating that lignin peroxidase is the key enzyme involved in degradation of MG in P. coccineus IUM0032.
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SangMi Han,KwangGill Lee,JooHong Yeo,SoonOk Woo,HaeYong Kweon,YouYoung Jo,Peter Molan 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Since the ancient times the therapeutic application of honeybee venom (BV) is practised and persisted until the present days. Resistant bacteria are in emergence and some drugs no longer have an antimicrobial action. To purify the melittin known as antibacterial peptide, five major peptidergic subfractions were separated, purified and identified from the whole BV. We investigated the antibacterial activity of whole BV and purified melittin against Staphylococcus aureus by the minimum inhibitory concentrations (MIC) and the postantibiotic effect (PAE). The MIC of whole BV for S. aureus was 0.06 ㎍/㎖, respectively. The MIC of melittin was 0.06 ㎍/㎖ on S. aureus. The in vitro PAE of whole BV and isolated melittin were determined using E. coli and S. aureus. The PAE of whole BV against S. aureus were 3.45 h (1×MIC). The PAE of melittin against S. aureus was 4.35 h (1 × MIC). Also both whole BV and melittin killed S. aureus at 5 × MIC. The regrowth wasn't observed after 18 h. These results suggest that whole BV and melittin will be developed a novel antibacterial drug.
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Lee, Changyeol,Kim, Soonok,Li, Wei,Bang, Sunghee,Lee, Hanna,Lee, Hyun-Jung,Noh, Eun-Young,Park, Jung-Eun,Bang, Woo Young,Shim, Sang Hee Springer Science and Business Media LLC 2017 Journal of antibiotics Vol.70 No.6
<P>Endophytes, important plant-associated mycobionts, have attracted a great deal of attention because of their bioactive secondary metabolites. Even though halophytes have been reported to overcome salt stress via associations with their endophytes, few studies have investigated the metabolites produced by the endophytes from halophytes. In this study, a dark septate endophytic fungal strain (JS0464), identified as Gaeumannomyces sp. by ITS sequencing, was isolated from the rhizome of a halophyte, Phragmites communis, in Suncheon bay, South Korea. This strain was cultured on a large scale and extracted with ethyl acetate. Chemical investigations of extracts of JS0464 led to the isolation of two glycosylated dialkylresorcinol derivatives (1-2), an anthraquinone derivative (3) and eight known compounds (4-11), which were identified by spectroscopic analyses incorporating one-dimensional/2D NMR and MS. Nine compounds showed significant nitric oxide reduction activity in lipopolysaccharide-stimulated microglia BV-2 cells, seven of which did not impair cell viability. The results suggest that endophytes from the halophytes could be potential resources for bioactive natural products.</P>
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Isolation of Abscisic Acid from Korean Acacia Honey with Anti- <i>Helicobacter pylori</i> Activity
Kim, SeGun,Hong, InPyo,Woo, SoonOk,Jang, HyeRi,Pak, SokCheon,Han, SangMi Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50
<P><B>Background:</B></P><P><I>Helicobacter pylori</I> (<I>H. pylori</I>) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide.</P><P><B>Objective:</B></P><P>This study is aimed at evaluating the anti-<I>H. pylori</I> activity of Korean acacia honey and isolating the related active components using organic solvents.</P><P><B>Material and Methods:</B></P><P>The crude acacia honey was extracted with <I>n</I>-hexane, dichloromethane, ethyl acetate (EtOAc), and <I>n</I>-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-<I>H. pylori</I> activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against <I>H. pylori</I> was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay.</P><P><B>Results:</B></P><P>Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36–72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against <I>H. pylori</I>.</P><P><B>Conclusion:</B></P><P>Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for <I>H. pylori</I>-induced infections.</P><P><B>SUMMARY</B></P><P><P>The crude acacia honey was extracted with <I>n</I>-hexane, dichloromethane, EtOAc, and <I>n</I>-butanol</P><P>The EtOAc extract yielded eight fractions and four subfractions were subsequently obtained chromatographically</P><P>Abscisic acid was isolated from one subfraction</P><P>All the solvent extracts and fractions showed antibacterial activity against <I>H. pylori</I></P><P>Abscisic acid exhibited antibacterial activity against <I>H. pylori</I>.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error.</P>
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한상미(Sangmi Han),우순옥(Soonok Woo),홍인표(Inpyo Hong),최용수(Youngsoo Choi),김정민(Joungmin Kim),조윤희(Yunhi Cho) 한국양봉학회 2012 韓國養蜂學會誌 Vol.27 No.2
The changes in quality and physiological activity of royal jelly (RJ) stored at room temperatures, 4°C, and -20°C under dark and light conditions for up to 6 months were investigated. The results showed that 10-HDA, total amino acid, free amino acid of RJ changed significantly during storage at room temperature both dark and light and 4°C, but not at -20°C. The physiological activity of RJ such as antioxidative activity using ABTS radical scavenging activity and nitric oxide scavenging activity at 50 μg/mL were decreased significantly during storage at room temperature and 4°C after 3 months, but not at -20°C. The human dermal fibroblasts (HDF) proliferations was decreased significantly durings torage at room temperature after 3 months, but not 4°C and -20°C. The results indicated that the quality deterioration and physiological activity of RJ during storage was due to the Maillard browning reaction.
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