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Sohae Park(박소혜) 한국생명과학회 2021 생명과학회지 Vol.31 No.8
태반유래 중간엽줄기세포(PD-MSCs)는 재생의학에서 세포기반치료제로 잘 알려진 세포군이다. PD-MSCs의 손상된 부위로의 이동과 호밍 기능은 MSC 생착의 중요한 특성이다. miRNA는 최근 MSC의 증식, 생존 이동과 같은 중요한 기능을 조절하는 것으로 알려져 있다. 본 연구의 목적은 담관결찰(BDL) 쥐 모델에서 PD-MSCs 호밍에 관련된 miRNA 및 표적 유전자를 동정하는 것으로, 마이크로어레이 분석을 이용하여 PD-MSCs 호밍에 관여하는 유전자 표적 miRNA를 선별하였다. BDL 쥐모델에 PD-MSCs을 이식한 일주일 후 간 조직에서 PD-MSCs 생착여부는 면역형광분석법과 qRT-PCR에 의한 인간 Alu유전자 발현으로 확인되었다. 저산소 및 정상조건(Hyp/Nor)에서 이동한 PD-MSC에 비하여, PD-MSCs 이식한 BDL군 간 조직에서 miRNAs 발현의 차이가 크게 나타났으며, PD-MSCs 호밍 관련 miRNA와 표적유전자를 검증하였다. miR199a-5p 및 miR-148a-3p에 대한 표적 유전자 인테그린 α4 (ITGA4)와 α5 (ITGA5)의 발현은 이식(Tx)그룹에서(p<0.05) 유의하게 상향 조절되었다. 또한 인테그린 β1 (ITGB1)과 β8 (ITGB8)의 발현은 miR-183-5p 및 miR-145-5p억제에 의하여 크게 증가되었다. 따라서 이러한 결과는 BDL에 의해 손상된 쥐간에서 PD-MSCs가 호밍효과을 위해 인테그린 그룹과 관련된 miRNA 발현 조절에 관여함을 나타내었다. 본 연구결과는 miRNA에 의한 인테그린 그룹 조절기능이 BDL에 의해 유도된 간섬유증 쥐모델에서 PD-MSCs의 치료효과에 기여할 수 있음을 시사한다. Placenta-derived mesenchymal stem cells (PD-MSCs) are promising candidates for cell-based therapy in regenerative medicine. The migration and homing potential of PD-MSCs to injured sites is a critical property of MSC engraftment. MicroRNAs (miRNAs) have recently been shown to regulate the critical functions of MSCs, such as proliferation, survival, and migration. The objective of the present study was to identify the miRNA and target genes involved in PD-MSCs homing in a bile duct ligation (BDL) rat model. We selected candidate miRNAs targeting genes for PD-MSCs homing based on microarray analysis. PD-MSC engraftment in BDL-injured rat liver was identified by immunofluorescence assay and human-specific Alu gene expression by quantitative real-time polymerase chain reaction (qRT-PCR) one week after transplantation. Compared with migrated naïve PD-MSCs under hypoxic and normoxic conditions (Hyp/Nor), the transplanted group with PD-MSCs (Tx) showed distinct differences in miRNA expressions in BDL-injured rat liver. We also validated the miRNAs and their target genes for PD-MSCs homing. The expressions of integrin α4 (ITGA4) and integrin α5 (ITGA5) target genes for miR-199a-5p and miR-148a-3p were significantly upregulated in the Tx group (p<0.05). In addition, integrin β1 (ITGB1) and integrin β8 (ITGB8) were upregulated by suppressing miR-183-5p and miR-145-5p, respectively. These results demonstrated that PD-MSCs regulate miRNA expression related to the integrin family for their homing effects on the BDL-injured rat liver. The findings further suggest that miRNA-mediated regulation of the integrin family contributes to the therapeutic efficacy of PD-MSCs in the rat hepatic fibrosis model by BDL.
다물체동역학기법을 이용한 고급버스의 전차량 시뮬레이션과 시험의 매칭
최소해(Sohae Choi),박성준(Seongjun Park),이정한(Jeonghan Lee),유완석(Wansuk Yoo),손정현(Jeonghyun Sohn) 한국자동차공학회 2010 한국 자동차공학회논문집 Vol.18 No.6
In this study, a large bus is tested for measuring the steering response based on the slarom test and step steer test. A full car model by using ADAMS/Car is established for computer simulation. For bus modeling, user defined templates are made and used in the simulation. Simulation results according to the slarom and step steer test are compared to the physical experiments, in which several sensors are installed to measure vehicle responses. The results obtained from the comparison show a good agreement with regard to the vehicle velocity and steering angle.
Neural Network Learning Method using Weight Mirroring and Direct Feedback Error
이소하(Soha Lee),양희성(Heesung Yang),박혜영(Hyeyoung Park) Korean Institute of Information Scientists and Eng 2024 정보과학회논문지 Vol.51 No.5
오류 역전파 알고리즘은 신경망의 핵심적인 학습 알고리즘으로 최근에도 다양한 딥러닝 모델에서 사용되고 있다. 그러나 오류 역전파 학습에서 상위층 오차 신호가 하위층으로 순차적으로 전달되면서 상위층의 가중치 정보가 하위층 가중치 업데이트에 사용되는 방식은 생물학적 타당성의 결여와 계산 효율성 저하의 문제가 지적되어왔다. 이러한 문제들을 개선하기 위하여 역방향 가중치를 별도로 사용하는 학습방법론들이 제안되었으나 아직 초기 연구에 머물고 있으며 다양한 관점에서의 분석이 필요하다. 본 논문에서는 상위층의 오차를 하위층으로 직접 투사하는 직접 피드백 정렬 방법과 별도의 역방향 가중치를 업데이트하는 단계를 가지는 가중치 미러 방법을 결합하여 새로운 학습 방법을 제안한다. 제안하는 방법은 기존의 두 알고리즘이 가지던 한계를 극복하여 생물학적으로 타당하며 효율적인 병렬 학습이 가능한 가중치 업데이트 방법을 구현한다. 여러 벤치마크 데이터에 대한 실험을 통하여 제안하는 방법의 가능성을 확인하였다. Error backpropagation algorithm is a core learning algorithm of neural networks and, until recently, has been used in various deep learning models. However, the weight update rule of error backpropagation, in which the error signal of the upper layer is sequentially transmitted to the lower layer and the weight values of the upper layer that are used to update the lower layer weights, has a problem of biological implausibility and computational inefficiency. To address these issues, learning methods using separate backward weights have been proposed, but they are still at an early stage and require further analysis and improvement from various perspectives. In this paper, we proposed a new learning method by combining the direct feedback alignment method, which directly projects the errors of the last layer into each hidden layer, and a weight mirror method with a separate step for updating backward weights. The proposed method overcomes the limitations of learning methods to implement a weight update method that is biologically plausible and allows for more efficient parallel learning. We confirmed the potential of the proposed method through experiments on various benchmark datasets.
So-Hyun Park,Kyungsun Kim,Soha Cho,Dong-Hwa Chung,Sug-Joon Ahn 대한치과교정학회 2022 대한치과교정학회지 Vol.52 No.4
Objective: To evaluate differences in the adhesion levels of the most common oral pathogens, Streptococcus mutans and Porphyromonas gingivalis, in human saliva-derived microcosm biofilms with respect to time and raw materials of orthodontic brackets. Methods: The samples were classified into three groups of bracket materials: 1) monocrystalline alumina ceramic (CR), 2) stainless steel metal (SS), and 3) polycarbonate plastic (PL), and a hydroxyapatite (HA) group was used to mimic the enamel surface. Saliva was collected from a healthy donor, and saliva-derived biofilms were grown on each sample. A real-time polymerase chain reaction was performed to quantitatively evaluate differences in the attachment levels of total bacteria, S. mutans and P. gingivalis at days 1 and 4. Results: Adhesion of S. mutans and P. gingivalis to CR and HA was higher than the other bracket materials (SS = PL < CR = HA). Total bacteria demonstrated higher adhesion to HA than to bracket materials, but no significant differences in adhesion were observed among the bracket materials (CR = SS = PL < HA). From days 1 to 4, the adhesion of P. gingivalis decreased, while that of S. mutans and total bacteria increased, regardless of material type. Conclusions: The higher adhesion of oral pathogens, such as S. mutans and P. gingivalis to CR suggests that the use of CR brackets possibly facilitates gingival inflammation and enamel decalcification during orthodontic treatment.