Photo Physical Studies of PVP Arrested ZnS Quantum Dots

Ashutosh Kumar Shahi,Bishnu Kumar Pandey,Bheeshma Pratap Singh,Bipin Kumar Gupta,Sukhvir Singh,Ram Gopal 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.2

Monodispersed polyvinylpyrrolidone (PVP) arrested ZnS quantumdots (QDs) having diameter in range ~2-5 nm are synthesized by acolloidal precipitation method using PVP as the stabilizing agent. X-ray diffraction (XRD), transmission electron microscopy (TEM),high resolution transmission electron microscopy (HRTEM),selective area electron diffraction (SAED) and Fourier transforminfrared (FT-IR) spectroscopy are probed to investigate thestructural information. The optical properties are studied usingdiffuse UV-visible reflectance and photoluminescence (PL)spectroscopy techniques. TEM images as well as XRD reflectionpeak broadening indicate the nanometer size particles formationwith cubic (sphalerite) phase within the polymer matrix. Opticalabsorbance studies reveal an excitonic peak at around ~310 nmdictates the effect of quantum confinement effect in the ZnS QDs. PL emission spectra for ZnS QDs in PVP exhibit four emissionpeaks at ~382 nm, ~414 nm, ~480 nm and ~527 nm are observed. These excitonic emissions from ZnS QDs are caused by theinterstitial sulfur/Zn vacancies and surface states.

Interleukin-1B (IL-1B-31 and IL-1B-511) and interleukin-1 receptor antagonist (IL-1Ra) gene polymorphisms in primary immune thrombocytopenia

Deependra Kumar Yadav,Anil Kumar Tripathi,Divya Gupta,Saurabh Shukla,Aloukick Kumar Singh,Ashutosh Kumar,Jyotsna Agarwal,K. N. Prasad 대한혈액학회 2017 Blood Research Vol.51 No.4

Background: Immune thrombocytopenia (ITP) is an immune-mediated disease caused by autoanti-bodies against platelets membrane glycoproteins GPIIb/IIIa and GPIb/IX. The etiology of ITP remains unclear. This study evaluated the association of polymorphisms in interleukin (IL)-1B-31, IL-1B-511, and IL-1Ra with ITP. Methods: Genotyping of IL-1B-31, IL-1B-511, and IL-1Ra was performed in 118 ITP patients and 100 controls by polymerase chain reaction restriction fragment length polymorphism and detection of variable number tandem repeats. Results: Genotype differences in IL-1B-31 and IL-1Ra were significantly associated with ITP. Patients showed a higher frequency of the IL-1B-31 variant allele (T) and a 1.52-fold greater risk of susceptibility to ITP (odds ratio [OR]=1.52, 95% confidence interval [CI]=1.04‒2.22, P=0.034). The frequencies of both homozygous and heterozygous variant geno-types of IL-1B-31 were higher (OR=2.33, 95% CI=1.069‒5.09, P=0.033 and OR=2.044, 95% CI=1.068‒39, P=0.034) among patients and were significantly associated with ITP susceptibility. Both homozygous and heterozygous variant genotypes of IL-1Ra were also more frequent (OR=4.48, 95% CI=1.17‒17.05, P=0.0230 and OR=1.80, 95% CI=1.03‒3.14, P=0.0494) among patients and were associated with ITP risk. IL-1B-31 and IL-1Ra also showed significant association with severe ITP. However, IL-1B-511 was not asso-ciated with ITP. Conclusion: IL-1B-31 and IL-1Ra polymorphisms may significantly impact ITP risk, and they could be associated with disease severity, which may contribute to the pathogenesis of ITP.

Interleukin-1B (IL-1B-31 and IL-1B-511) and interleukin-1 receptor antagonist (IL-1Ra) gene polymorphisms in primary immune thrombocytopenia

Deependra Kumar Yadav,Anil Kumar Tripathi,Divya Gupta,Saurabh Shukla,Aloukick Kumar Singh,Ashutosh Kumar,Jyotsna Agarwal,K. N. Prasad 대한혈액학회 2017 Blood Research Vol.52 No.4

Background: Immune thrombocytopenia (ITP) is an immune-mediated disease caused by autoanti-bodies against platelets membrane glycoproteins GPIIb/IIIa and GPIb/IX. The etiology of ITP remains unclear. This study evaluated the association of polymorphisms in interleukin (IL)-1B-31, IL-1B-511, and IL-1Ra with ITP. Methods: Genotyping of IL-1B-31, IL-1B-511, and IL-1Ra was performed in 118 ITP patients and 100 controls by polymerase chain reaction restriction fragment length polymorphism and detection of variable number tandem repeats. Results: Genotype differences in IL-1B-31 and IL-1Ra were significantly associated with ITP. Patients showed a higher frequency of the IL-1B-31 variant allele (T) and a 1.52-fold greater risk of susceptibility to ITP (odds ratio [OR]=1.52, 95% confidence interval [CI]=1.04‒2.22, P=0.034). The frequencies of both homozygous and heterozygous variant geno-types of IL-1B-31 were higher (OR=2.33, 95% CI=1.069‒5.09, P=0.033 and OR=2.044, 95% CI=1.068‒39, P=0.034) among patients and were significantly associated with ITP susceptibility. Both homozygous and heterozygous variant genotypes of IL-1Ra were also more frequent (OR=4.48, 95% CI=1.17‒17.05, P=0.0230 and OR=1.80, 95% CI=1.03‒3.14, P=0.0494) among patients and were associated with ITP risk. IL-1B-31 and IL-1Ra also showed significant association with severe ITP. However, IL-1B-511 was not asso-ciated with ITP. Conclusion: IL-1B-31 and IL-1Ra polymorphisms may significantly impact ITP risk, and they could be associated with disease severity, which may contribute to the pathogenesis of ITP.

Genome-wide identification and analysis of Subtilisin-like serine protease gene family in banana (Musa accuminta L.) and their expression under abiotic stresses

Purwar Shalini,Chugh Vishal,Singh Poornima,Srivastava Akhilesh Kumar,Singh Akhilesh Kumar,Mishra Akhilesh Chandra,Singh Ashutosh,Singh Chandra Mohan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Subtilisin-like serine proteases (SBT), a serine proteolytic enzymes play an important role in plant growth function and during different stresses responses. The systematic analysis of the SBT gene family in Musa acuminate (MaSBT) has been done and their responses to abiotic stresses in banana variety cv. G-9 were also analyzed. Total of 67 MaSBT genes were identified and based on phylogeny these were grouped into five districted subgroups. Cis-acting element analysis indicated that almost all MaSBT promoters contain regulatory elementary related to growth and development, hormonal regulation, and stress responses. The gene structure and domain analysis showed a maximum of seventeen exons and four functional domains in MaSBT. The 42 orthologous genes, 07 MaSBT paralogous genes were also identified through synteny analysis. The Ka /Ks study indicated that four MaSBT paralogous gene pairs were tandemly duplicated, while the other three were segmental duplications. Further, the expression pattern via RNA-seq data revealed that MaSBTs exhibited differential expres- sion specifically in response to the abiotic stress of low nitrogen. and also during the flowering time. The MaSBT-1.7 gene was found involved in the response to salt stress and flowering. These findings establish a cornerstone for future research on banana's salt stress mechanism. The study offers valuable insights into SBT encoding genes, shedding light on their roles in growth, development, and abiotic stress responses

Resource-efficient load-balancing framework for cloud data center networks

Kumar, Jitendra,Singh, Ashutosh Kumar,Mohan, Anand Electronics and Telecommunications Research Instit 2021 ETRI Journal Vol.43 No.1

Cloud computing has drastically reduced the price of computing resources through the use of virtualized resources that are shared among users. However, the established large cloud data centers have a large carbon footprint owing to their excessive power consumption. Inefficiency in resource utilization and power consumption results in the low fiscal gain of service providers. Therefore, data centers should adopt an effective resource-management approach. In this paper, we present a novel load-balancing framework with the objective of minimizing the operational cost of data centers through improved resource utilization. The framework utilizes a modified genetic algorithm for realizing the optimal allocation of virtual machines (VMs) over physical machines. The experimental results demonstrate that the proposed framework improves the resource utilization by up to 45.21%, 84.49%, 119.93%, and 113.96% over a recent and three other standard heuristics-based VM placement approaches.

Diagnostic Evaluation of Non-Interpretable Results Associated with rpoB Gene in Genotype MTBDRplus Ver 2.0

( Binit Kumar Singh ),( Rohini Sharma ),( Parul Kodan ),( Manish Soneja ),( Pankaj Jorwal ),( Neeraj Nischal ),( Ashutosh Biswas ),( Sanjay Sarin ),( Ranjani Ramachandran ),( Naveet Wig ) 대한결핵 및 호흡기학회 2020 Tuberculosis and Respiratory Diseases Vol.83 No.4

Background: Line probe assay (LPA) is standard diagnostic tool to detect multidrug resistant tuberculosis. Noninterpretable (NI) results in LPA (complete missing or light wild-type 3 and 8 bands with no mutation band in rpoB gene region) poses a diagnostic challenge. Methods: Sputum samples obtained between October 2016 and July 2017 at the Intermediate Reference Laboratory, All India Institute of Medical Sciences Hospital, New Delhi, India were screened. Smear-positive and smear-negative culturepositive specimens were subjected to LPA Genotype MTBDRplus Ver 2.0. Smear-negative with culture-negative and culture contamination were excluded. LPA NI samples were subjected to phenotypic drug susceptibility testing (pDST) using MGIT-960 and sequencing. Results: A total of 1,614 sputum specimens were screened and 1,340 were included for the study (smear-positive [n=1,188] and smear-negative culture-positive [n=152]). LPA demonstrated 1,306 (97.5%) valid results with TUB (Mycobacterium tuberculosis) band, 24 (1.8%) NI, three (0.2%) valid results without TUB band, and seven (0.5%) invalid results. Among the NI results, 22 isolates (91.7%) were found to be rifampicin (RIF) resistant and two (8.3%) were RIF sensitive in the pDST. Sequencing revealed that rpoB mutations were noted in all 22 cases with RIF resistance, whereas the remaining two cases had wild-type strains. Of the 22 cases with rpoB mutations, the most frequent mutation was S531W (n=10, 45.5%), followed by S531F (n=6, 27.2%), L530P (n=2, 9.1%), A532V (n=2, 9.1%), and L533P (n=2, 9.1%). Conclusion: The present study showed that the results of the Genotype MTBDRplus assay were NI in a small proportion of isolates. pDST and rpoB sequencing were useful in elucidating the cause and clinical meaning of the NI results.

Perioperative duloxetine as part of a multimodal analgesia regime reduces postoperative pain in lumbar canal stenosis surgery: a randomized, triple blind, and placebo-controlled trial

Nishith Govil,Kumar Parag,Pankaj Arora,Hariom Khandelwal,Ashutosh Singh,Ruchi 대한통증학회 2020 The Korean Journal of Pain Vol.33 No.1

Background: Duloxetine is an antidepressant that is also useful in chronic neuropathic and central origin pain. In this study, the role of duloxetine in decreasing acute postoperative pain after lumbar canal stenosis surgery is explored. Methods: In this single center, triple blinded, and placebo-controlled trial, 96 patients were randomized for statistical analysis. The intervention group received oral duloxetine 30 mg once a day (OD) for 2 days before surgery, 60 mg OD from the day of surgery to the postoperative second day and 30 mg OD for the next 2 days (a total duration of 7 days). A placebo capsule was given in the other group for a similar time and schedule. The same standard perioperative analgesia protocols were followed in both groups. Results: Total morphine consumption up to 24 hours was significantly decreased in the duloxetine group (P < 0.01). The time to the first analgesia requirement was similar in both groups but the time to the second and third dose of rescue analgesia increased significantly in the duloxetine group. The time to ambulation was decreased significantly (P < 0.01) in the duloxetine group as compared to the placebo group. Pain scores remained similar during most of the time interval. No significant difference was observed in the complication rate and patient satisfaction score recorded. Conclusions: Duloxetine reduces postoperative pain after lumbar canal stenosis surgery with no increase in adverse effects.

Challenges, Configuration, Control, and Scope of DC Microgrid Systems: A Review

Maurya Rinki,Prakash Surya,Singh Ashutosh Kumar 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

Managing natural resources and air pollution has been challenging for humans for quite a long time. A severe manifestation of natural resource mismanagement has been in the form of CO2 emissions from smoke bellowing thermal power plants (TPPs). Besides evoking global warming, the TPPs also foster limited coal reserve reduction with the increasing electricity demand. The ongoing scenario may lead to severe catastrophes until efficient plans for natural resource management are prepared. Many researchers have suggested DC microgrid as a credible alternative for power generation, significantly reducing carbon emissions. Efficient control strategies have brought microgrid technology to the level of other generation sources in terms of system reliability and efficiency. Renewable energy resources can be implemented as a safe, low voltage (< 50 V) local DC microgrid for DC load, reducing the converter requirement with low transmission losses and overcoming the scarcity of electricity. Seamless integration of solar-PV systems and batteries into the DC microgrid will increase the predominance of clean electricity in the modern power system. It will alleviate the air pollution problems generated by TPPs and sequels to enable natural resource management effectively. In light of the above facts, this paper presents a detailed survey on the challenges, configuration, control, and scope of DC microgrid systems. Various predominant configurations, recent advances in control strategies, operation challenges, and DC microgrid commissioning have been discussed. Moreover, a case study illustrating the benefits of DC systems is presented along with the energy-saving analysis compared with AC systems.

Blistering kinetics in H-implanted 4H-SiC for large-area exfoliation

Sharma Madan,Soni K.K.,Kumar Ashutosh,Ohkubo Tadakatsu,Kapoor A.K.,Singh Rajendra 한국물리학회 2021 Current Applied Physics Vol.31 No.-

Layer transfer of single-crystalline SiC based on layer splitting and wafer bonding on an alternate substrate is a viable approach to fabricate SiC power devices at a lower cost. Dependence of implantation-induced surface blistering and exfoliation of thin layers, on the implantation parameters and subsequent annealing conditions, for large area exfoliation of 4H-SiC have been studied. We report the optimization of the annealing process (one-step or two-step) and annealing parameters like temperature and time to achieve large area exfoliation of SiC. Using the F¨oppl-von Karman (FvK) theory, the pressure inside the blister cavities and implantation-induced stress inside the damage region have been studied. We deduce that pressure inside the blister depends primarily on the blister size. HRXRD analysis has been used to determine the strain relaxation behavior as a function of annealing conditions. Raman spectroscopy and STEM measurements revealed the formation of defects inside the material due to ion implantation. AFM, Nomarski, and SEM were used to measure the size and depth details of the blisters as a function of annealing temperature and time. We have successfully exfoliated large area (up to 500 μm) in a two-step annealing process. The results reported in this paper are useful for SiC layer transfer on an arbitrary substrate by the Smart Cut process.