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RISS 인기검색어
- 검색키워드
- 저자 : Shuqin Yan (검색결과 3 건)
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Mingquan Wang,Yufeng Wang,Yifei Zhang,Chunxia Li,Shichen Gong,Shuqin Yan,Guoliang Li,Guanghui Hu,Honglei Ren,Jianfei Yang,Tao Yu,Kejun Yang 한국유전학회 2019 Genes & Genomics Vol.41 No.7
Background Salt stress is a devastating environmental stress that causes plant growth inhibition and yield reduction. Objective The identification of salt-tolerant genes brings hope for the generation of salinity-tolerant crop plants through molecular breeding. Methods In this study, one salt-sensitive and one salt-tolerant maize inbred line were screened from 242 maize inbred lines. Reactive oxygen species (ROS)-related enzyme activities were detected and salt-responsive comparative transcriptome analysis was performed for control and 220 mM NaCl treated maize leaves. Results Salt-tolerant maize inbred line (L87) showed higher ROS-related enzyme (SOD, POD, APX and CAT) activities and accumulated relatively lower levels of ROS under salt stress. Of the total DEGs, 1856 upregulated DEGs were specific to L87, including stress tolerance-related members of the 70kDa family of heat shock proteins (Hsp70s) and aquaporins. The DEGs involved in the abscisic acid (ABA), ethylene, jasmonic acid (JA) and salicylic acid (SA) signal transduction pathways may determine the difference in salt tolerance between the two varieties, especially one central component SnRK2, that positively regulates ABA signaling and was only upregulated in L87. Analysis of DEGs related to ROS scavenging showed that some peroxidase (POD), glutathione S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) genes specific to L87 probably enhanced its salt tolerance. The analysis of differentially expressed transcription factors (TFs) suggested that WRKY TFs could contribute to the difference in salt tolerance between the two maize lines. Conclusion Compared with Salt-sensitive maize inbred line (L29), L87 exhibits specific regulatory mechanisms related to salt tolerance, including plant hormone interactions, ROS scavenging and the regulation of TFs. Our study identifies new candidate genes that may regulate maize tolerance to salt stress and provides useful information for breeding maize with high salt resistance.
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Chunyu Zhu,Fangliang Zheng,Sugai Yin,Hongsheng Liu,Yan Liu,Maosheng Zhang,Shuqin Xiong 한국유전학회 2014 Genes & Genomics Vol.36 No.6
The baculovirus ChaB proteins are conservedin all completely sequenced Lepidopteran NPVs and areannotated as putative DNA binding proteins. In our previousstudy, ORF51, a ChaB homologue from Helicoverpaarmigera single nucleocapsid nucleopolyhedrovirus(HearNPV) was found to be involved in budded virusproduction and DNA replication. In the present study, wecharacterized ORF52 (Ha52), the other ChaB homologousgene in HearNPV. 50-RACE revealed that Ha52 was transcribedfrom a conventional late promoter transcriptionalinitiator motif (TAAG) located 25 nucleotides upstream ofATG. Expression analysis demonstrated that HA52 proteinwas expressed from 48 to 96 h post infection. Western blotanalysis of virions from both budded viruses (BVs) andocclusion-derived viruses (ODVs) indicated that HA52 wasa structural component of nucleocapsid from ODV. Tostudy the function of Ha52 in the life cycle of HearNPV,Ha52-knockout and Ha52-restored viruses were generatedby the Bac-to-Bac system. Growth curve analyses showedthat the level of BV production in cells infected with Ha52null virus was similar to those infected with wild-typebacmid derived virus. However, bioassay showed thatdeletion of Ha52 significantly decreased the per os infectivityof HearNPV. Taken together, our results indicatedthat, unlike the previously characterized baculovirual ChaBgenes, Ha52 may be involved in HearNPV per os infection.
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Facile Preparation of Mechanical Reinforced and Biocompatible Silk Gels
Qiang Zhang,Guocong Han,Chen Lu,Qiusheng Wang,Xiufang Li,Zuwei Luo,Renchuan You,Shuqin Yan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4
Nontoxic and controllable way to fabricate silk fibroin (SF) gel with high mechanical properties is of critical important to biomaterial in tissue engineering. Electrochemically triggered electrophoretic migration and electric charge of the silk molecules both contributed to SF microspheres and electronic gel (e-gel) formation by sol-gel transition. In this study, a novel silk pH e-gel with higher mechanical property was prepared by combining low-voltage electric fields with isoelectric point (pI) adjustment. This green process was mild and friendly without chemical crosslinker. Compressive modulus of the silk pH e-gel was up to 70 MPa that was significantly higher than that of SF gelation spontaneously. Furthermore, analysis of molecule conformation of the silk pH-e-gel demonstrated that most of random coil structures transformed into α-helix and a little β-sheet structures during this process. The silk pH e-gel was loaded with rhodamine B and showed an obvious sustainable release profile. Accumulation releasing amounts was approximately 60 % at day 9. Cytocompatibility of the silk pH-e-gel was evaluated by epithelial cell. The results showed that the gels could support the cell growth and proliferation in vitro. Finally, gel biodegradation was assessed by protease XIV. After biodegradation for 28 days, remaining weight of the gel was about 20.23±2.59 wt%, indicating its good biodegradability. This novel process was established successfully by combining low voltage field with pH-control, which provided an alternative material for regenerative medicine.
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