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      • KCI등재

        An ultra-thin dual-band wide-angle polarization-insensitive metamaterial absorber with near-unity absorbance

        Shijun Ji,Chengxin Jiang,Ji Zhao,Jilong Yang,Jili Wang,Handa Dai 한국물리학회 2019 Current Applied Physics Vol.19 No.11

        In this paper, a dual-band metamaterial absorber (MMA) with wide-angle and polarization-insensitivity is proposed. The MMA consists of two copper layers with a layer of FR-4 between them. And its top layer consists of a cross-shaped resonator and a square ring resonator. The calculation result demonstrates that there are two distinct absorption peaks, whose absorptivity are 99.933% at 3.8441 GHz and 99.99% at 9.1094 GHz. And its thickness is only 1.34% of the wavelength of the lowest absorption frequency. The dual-band MMA shows polarization-insensitivity for normal incident wave and shows high absorptivity in a wide incident angle for both TE and TM polarization wave. In addition, we discuss the working mechanism. The influences of main parameters on the dual-band MMA's absorption are also analyzed. The proposed ultra-thin MMA has simple structure and high absorptivity, which has many potential applications, such as thermal radiometer, detection sensor, stealth technology.

      • KCI등재

        Interfacial Reaction in (Mg-37.5Al)/(Mg-6.7Nd) Diffusion Couples

        Jiahong Dai,Shijun Shen,Bin Jiang,Jianyue Zhang,Qingshan Yang,Zhongtao Jiang,Hanwu Dong,Fusheng Pan 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.1

        The solid-state diffusion reaction between Mg-37.5Al and Mg-6.7Nd was studied in the 623-673 K temperature range. A solid-liquid contact method was employed to produce diffusion couples. The Al4Nd, Al11Nd3, Al3Nd and Al2Nd intermetallic compounds form in the diffusion reaction layers of the couples. The formation of intermetallic compounds in the diffusion reaction layers is rationalized using the Miedema model. The local average compositions through the diffusion reaction layer were determined, and used to construct a semi-quantitative diffusion path on the isothermal Mg-Al-Nd ternary phase diagram at 673 K. The growth constants of the entire diffusion reaction layers were determined as 8.91 (±0.94)×10 -14 m 2 /s, 1.39 (±0.15)×10 -13 m 2 /s and 1.93 (±0.38)×10 -13 m 2 /s at 623 K, 648 K and 673 K, respectively. The activation energy Q for the growth of the entire diffusion reaction layers was 54±4.6 kJ/mol.

      • SCIEKCI등재

        A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

        Liu, Na,Jiang, Shijun,Feng, Songli,Shang, Wenyan,Xing, Guozhen,Qiu, Rui,Li, Chengjun,Li, Shujun,Zheng, Wenming The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.2

        A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and ${\beta}$-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was $100fg/{\mu}l$ of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

      • KCI등재

        A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

        Na Liu,Shijun Jiang,Songli Feng,Wenyan Shang,Guozhen Xing,Rui Qiu,Chengjun Li,Shujun Li,Wenming Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2

        A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

      • SCIESCOPUSKCI등재

        Production of Transgenic Pigs with an Introduced Missense Mutation of the Bone Morphogenetic Protein Receptor Type IB Gene Related to Prolificacy

        Zhao, Xueyan,Yang, Qiang,Zhao, Kewei,Jiang, Chao,Ren, Dongren,Xu, Pan,He, Xiaofang,Liao, Rongrong,Jiang, Kai,Ma, Junwu,Xiao, Shijun,Ren, Jun,Xing, Yuyun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.7

        In the last few decades, transgenic animal technology has witnessed an increasingly wide application in animal breeding. Reproductive traits are economically important to the pig industry. It has been shown that the bone morphogenetic protein receptor type IB (BMPR1B) A746G polymorphism is responsible for the fertility in sheep. However, this causal mutation exits exclusively in sheep and goat. In this study, we attempted to create transgenic pigs by introducing this mutation with the aim to improve reproductive traits in pigs. We successfully constructed a vector containing porcine BMPR1B coding sequence (CDS) with the mutant G allele of A746G mutation. In total, we obtained 24 cloned male piglets using handmade cloning (HMC) technique, and 12 individuals survived till maturation. A set of polymerase chain reactions indicated that 11 of 12 matured boars were transgene-positive individuals, and that the transgenic vector was most likely disrupted during cloning. Of 11 positive pigs, one (No. 11) lost a part of the terminator region but had the intact promoter and the CDS regions. cDNA sequencing showed that the introduced allele (746G) was expressed in multiple tissues of transgene-positive offspring of No.11. Western blot analysis revealed that BMPR1B protein expression in multiple tissues of transgene-positive $F_1$ piglets was 0.5 to 2-fold higher than that in the transgene-negative siblings. The No. 11 boar showed normal litter size performance as normal pigs from the same breed. Transgene-positive $F_1$ boars produced by No. 11 had higher semen volume, sperm concentration and total sperm per ejaculate than the negative siblings, although the differences did not reached statistical significance. Transgene-positive $F_1$ sows had similar litter size performance to the negative siblings, and more data are needed to adequately assess the litter size performance. In conclusion, we obtained 24 cloned transgenic pigs with the modified porcine BMPR1B CDS using HMC. cDNA sequencing and western blot indicated that the exogenous BMPR1B CDS was successfully expressed in host pigs. The transgenic pigs showed normal litter size performance. However, no significant differences in litter size were found between transgene-positive and negative sows. Our study provides new insight into producing cloned transgenic livestock related to reproductive traits.

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