Integrative metabolome and transcriptome analyses reveal the differences in flavonoid and terpenoid synthesis between Glycyrrhiza uralensis (licorice) leaves and roots

Kaiqiang Yu,Li Peng,Wenyu Liang,Jing Shi,Guoqi Zheng,Hong Wang,Xinhua Liang,Shijie Wu 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

Licorice from Glycyrrhiza uralensis roots is used in foods and medicines. Although we are aware that licorice roots and leaves have distinct material compositions, the specific reasons for these differences remain unknown. Comparison of the metabolomes and transcriptomes between the leaves and roots revealed flavonoids and triterpenoid saponins were significantly different. Isoflavones were enriched in roots because of upregulation of genes encoding chalcone isomerase and flavone synthase, which are involved in isoflavone synthesis. Six triterpenoid saponins were significantly enriched only in the roots. The leaves did not accumulate glycyrrhetinic acid because of low expression levels of genes involved in its synthesis. A gene encoding a UDP glycosyltransferase, which likely catalyzes the key step in the transformation of glycyrrhetinic acid to glycyrrhizin, was screened. Our results provide information about the differences in flavonoid and triterpenoid synthesis between roots and leaves, and highlight targets for genetic engineering.

Extraction of visual texture features of seabed sediments using an SVDD approach

Li, Yan,Liu, Shijie,Zhu, Puqiang,Yu, Jiancheng,Li, Shuo Pergamon Press 2017 Ocean engineering Vol.142 No.-

<P><B>Abstract</B></P> <P>Perception of the seabed environment is an important capability of autonomous underwater vehicles. This paper focuses on defining and extracting robust texture features from visual images that lead to useful and practical automated identification of the types of seabed sediments. The visual texture features are described by using a gray-level co-occurrence matrix (GLCM) and fractal dimension, after which an unsupervised learning method, self-organizing map (SOM), is adopted to evaluate the validity of features descriptors on three types of seabed sediments. Subsequently, a kernel-based approach that exhibits robustness versus low numbers of high-dimensional samples, named support vector domain description (SVDD), is applied to classify the types of seabed sediments. In comparison with state-of-the-art classifiers, the experimental results demonstrated the effectiveness of the SVDD on the classification of seabed sediments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The visual images of seabed sediments are characterized by the texture features which are extracted based on the GLCM and fractal theory. </LI> <LI> A multi-class classification strategy for seabed sediments is proposed by adding a distance measure after SVDD implementation. </LI> <LI> The experimental results demonstrate that the proposed classification strategy is feasible in recognizing the type of seabed sediments. </LI> </UL> </P>

Investigation on the stability of periodic motions of a flexible rotor-bearing system with two unbalanced disks

Chaofeng Li,Shihua Zhou,Shijie Jiang,Hexing Yu,Bang-chun Wen 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.7

The stability of periodic motion of a rotor-bearing system with two unbalanced disks is analyzed based on a multi-degree of freedom(M-DOF) finite element model. Nonlinear effects of supporting oil-film and inertia distributions as well as shearing effect are taken intoaccount. Focusing on the more realistic FE method to study the stability of nonlinear rotor-bearing system with the influence of the eccentricityphase, the stability calculation of the M-DOF FE model is done by using the combination of Shooting method and Newmarkmethod. Experiments with a rotor-bearing test rig (two disks on a shaft with two bearings) are then performed and results are presented. Itis found that typical instability characteristic is successfully produced. Through comparisons between calculated results and measurements,it is shown that the combination of Shooting method and Newmark method in studying the M-DOF rotor-bearing system is useful,especially for the complex rotor-bearing system. In addition, altering eccentricity phase of the two disks can significantly influence instabilitytype and speed. When the two disks are out of phase, the occurring of oil whip is restrained effectively. It is suggested that a carefulexamination should be made in modeling more complex non-linear rotor-bearing system.

Estimation of remaining energy and available power for Li‑Ion battery packs considering energy consumption by heat convection

Yafeng Zheng,Chunyu Wang,Shijie Sang,Suoqing Yu 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.1

To realize the efficient use of battery residual energy, this paper attempts to estimate both the state of energy (SoE) and the state of available power (SoAP) for li-ion battery packs. First, the parameters of a 1st-order equivalent circuit model are identified online where the charging and discharging resistances are separately modeled. Then a state of energy estimator, considering the energy dissipation by heat convection, is designed using an unscented particle filter. Afterwards, multiple constraints in terms of cut-off voltages, recommended residual energy, extreme currents, and powers are incorporated to aid in SoAP prediction. Experiments on a 4-cell battery pack using a high-dynamic load profile show that the SoE estimator is reliable against various working conditions. The predicted SoAP with different time horizons and at different temperatures can avoid the conflicts with the preset constraints while giving reliable predictions.

Microstructural Evolution of a High W Content Ni-Based Superalloy at γ′ Sub-Solvus Temperatures

Sigeng Zhou,Naicheng Sheng,Shijie Sun,Shigang Fan,Jinjiang Yu,Guichen Hou,Jinguo Li,Yizhou Zhou,Xiaofeng Sun 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.1

The evolution of γ′ phase and γ/γ′ eutectic during short-time heat treatment of the Ni-based superalloy with high W content,K416B are investigated. In this study, high temperature Differential Scanning Calorimetry (DSC) is employed to designthe heat treatment experiments and the precipitate temperature of secondary γ′ is found in the cooling curve. The standardcoarsening model is applied to figure out the coarsening rate constant of γ′ precipitate. On this basis, a novel method isproposed to predict the globularization time of γ phase in γ/γ′ eutectic during the heat treatment process. As holding temperatureincreases, more γ lamellae convert to platelets before reaching thermodynamic equilibrium. Electron microscopyobservations exhibit the boundary splitting and necking formation on the γ plate surface. Moreover, the dissolution kineticsof γ phase is quantitatively assessed by JMAK model. The volume fraction descends rapidly in the initial stage, and thenreach to a threshold. Above proposed approaches are validated via various heat treatment experiments.

Regulating the magnetic anisotropy by Hf thickness and heat treatment in Pt/Co/Hf films

Li Minghua,Zhang Shijie,Wang Shuanghai,Li Yongzeng,Liu Lu,Chen Yu,Shi Hui,Yu Guanghua 한국물리학회 2022 Current Applied Physics Vol.34 No.-

Pt/Co/Hf multilayers were prepared by magnetron sputtering, and the magnetic anisotropy was effectively regulated by Hf thickness and heat treatment in Pt/Co/Hf films. The interface microstructures were characterized. The influence of the interface microstructure on magnetic properties was studied. The results show that the magnetic anisotropy in Pt/Co/Hf films is closely related to the interface microstructure, which is influenced by Hf thickness and the heat treatment temperature. Microstructure analysis shows that after the Pt(3)/Co(1.5)/Hf (1) film is heat-treated, the CoOx content increases, more CoPt(111) forms, the interface is smoother and sharper, and the roughness of the Co/Hf interface decreases. Several factors work together to cause the magnetic anisotropy of the sample to change from in-plane magnetic anisotropy (IMA)to perpendicular magnetic anisotropy (PMA).

Facile synthesis of AgPt nano-pompons for efficient methanol oxidation: Morphology control and DFT study on stability enhancement

Tao Shao,Dehong Bai,Mingzhu Qiu,Yu Li,Qiankun Zhang,Ziyu Xue,Shijie He,Dongxia Zhang,Xibin Zhou 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-

Facile synthesis of more dendritic and uniform Pt-based nanostructures with carbon materials couldgreatly reduce cost and increase Pt utilization for methanol oxidation reaction (MOR) in direct methanolfuel cell (DMFC). This study reports a novel one-pot method to fabricate AgPt nano-pompons (AgPt NPs)with the guidance of N-GQDs through AA reduce the precursor of Ag and Pt. Morphology characterizationdescribes N-GQDs as morphology guiding and dispersing agents to regulate the dendrite formation ofnano-pompons. Under the optimized conditions, the AgPt NPs (Ag1Pt2) display above 11 times improvementin electrocatalytic activity and higher stability for the MOR compared with Pt/C catalysts. Densityfunction theory (DFT) studies prove that the introduction of Ag can greatly enhance the adsorption of COon Pt and promote the transformation of CO to COOH. The facile synthetic method and excellent MORperformance endow AgPt NPs with great application prospect in DMFCs as an anode catalyst.

Plant derived cationic dye as an effective corrosion inhibitor for 7075 aluminum alloy in 3.5% NaCl solution

Ambrish Singh,Yuanhua Lin,Wanying Liu,Shijie Yu,Jie Pan,Chengqiang Ren,Deng Kuanhai 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6

The inhibition of the corrosion of 7075 aluminum alloy in 3.5 wt.% NaCl solution by 5,6-dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolo[5,6-a]quinolizinium (berberine) has been studied using electrochemicalimpedance spectroscopy (EIS), potentiodynamic polarization, scanning electrochemicalmicroscopy (SECM) and scanning electron microscopy (SEM). Inhibition efficiency was found toincrease with increasing concentration of berberine. The adsorption of the berberine on the 7075aluminum alloy surface obeyed the Langmuir adsorption isotherm. The adsorbed film on 7075aluminum alloy surface containing inhibitor was confirmed by the SEM, and SECM. The results obtainedshowed that the berberine could serve as an effective inhibitor of the corrosion of 7075 aluminum alloyin 3.5% NaCl.

Biodegradable Magnesium Alloy Stents as a Treatment for Vein Graft Restenosis

Yugang Li,Lei Wang,Shanshan Chen,Dan Yu,Weifeng Sun,Shijie Xin 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5

Purpose: To explore the effects of biodegradable magnesium alloy stents (BMAS) on remodeling of vein graft (VG) anastomoticrestenosis. Materials and Methods: To establish a VG restenosis model, seventy two New Zealand rabbits were randomly divided into threegroups according to whether a stent was implanted in the graft vein or not. BMASs and 316L stainless steel stents were implantedin BMAS and 316L groups, respectively, while no stent was implanted in the no-treatment control group (NC group). Loss of lumendiameter in the graft vein was measured in all three groups. Upon harvesting VG segments to evaluate intimal proliferationand re-endothelization, the degradation and biological safety of the stents were observed to explore the effects of BMAS on VGremodeling. Results: Model establishment and stent implantation were successful. The BMAS reduced lumen loss, compared with the controlgroup (0.05±0.34 mm vs. 0.90±0.39 mm, p=0.001), in the early stage. The neointimal area was smaller in the BMAS group than the316L group after 4 months (4.96±0.66 mm2 vs. 6.80±0.69 mm2, p=0.017). Re-endothelialization in the BMAS group was better thanthat in the 316L group (p=0.001). Within 4 months, the BMAS had degraded, and the magnesium was converted to phosphorusand calcium. The support force of the BMAS began to reduce at 2–3 months after implantation, without significant toxic effects. Conclusion: BMAS promotes positive remodeling of VG anastomosis and has advantages over the conventional 316L stents in thetreatment of venous diseases.

Characteristics analysis of rotor-rolling bearing coupled system with fit looseness fault and its verification

Wang Haifei,Guan Xiaoying,Chen Guo,Gong Junjie,Yu Liang,Yuan Shijie,Zhu Zhida 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

Outer ring of bearing fit looseness fault is a common fault. Scratch often appears in the inner surface of pedestal. The fit looseness fault mechanism is not clear. For rotor-rolling bearing system with fit looseness fault between rotor-bearing outer ring and pedestal, a rotor coupling dynamic model that the interaction of bearing outer ring and pedestal are considered. This model is different from the universal rubbing model, where the directions of relative motion between rotor and stator are not considered. Numerical integration method is used to obtain the response of the system where the rotor is established by FEM and the bearing outer ring and pedestal are established by lumped mass model. Firstly, modal test results and simulation results were used to verify the correctness of this model. Secondly, the role of tightening torque between bearing outer ring and pedestal is considered, and the response characteristics of bearing and rotor are analyzed when fit looseness fault is considered. Finally, comparing the simulation results with test results, the waveform and spectrum are similar, which verifies the correctness of the fit looseness model. The fit looseness fault characteristics are that the acceleration after noise reduction shows periodic impact, up and down asymmetry, multiple frequencies appear. A method by increasing tightening torque is put forward to control the vibration caused by fit looseness fault.