Autologous Bone-marrow Mesenchymal Cell induced Chondrogenesis: Single-stage Arthroscopic Cartilage Repair

Asode Ananthram Shetty,김석중,Vishvas Shetty,David Stelzeneder,Neha Shetty,Praveen Bilagi,이현진 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.3

We describe a single stage arthroscopic procedure for the treatment of articular cartilage defects in theknee. The novel procedure involves microfracture and application of bone marrow aspirate concentrate cells(BMAC) with hyaluronic acid and fibrin gel. The aim of the study was to evaluate the clinical and radiological out-comes at 2 years. A prospective study of 30 patients with symptomatic ICRS grade III/IV chondral defects, rangingfrom 2-9cm2, who were assessed clinically and radiologically. The surgical procedure involved debridement of thelesion, microfracture and application of concentrated BMAC with HA and fibrin gel under CO2 insufflation. Patientsunderwent morphological MRI, quantitative T2*-mapping and d-GEMRIC scan. Clinical assessment used the Lysh-olm, IKDC and KOOS scores. Radiological assessment used the MOCART score. At 2 year follow-up, Lysholmscore was 80.1, as compared to 50.8 pre-operatively (p<0.05). KOOS (symptomatic) was 92.1, as compared to 65.7pre-operatively. IKDC (subjective) was 83, up from 39 preoperatively. The mean T2* relaxation-times for the repairtissue and native cartilage were 29.1 and 29.9 respectively. Average MOCART score for all lesions was 72. Our tech-nique shows encouraging clinical results at 2 year follow-up. Clinical outcome scores show significant benefit. Themorphological MRI shows good cartilage defect filling and the biochemical MRI (T2*-mapping) suggests hyalinelike repair tissue.

Cone-beam computed tomography characterization of the intraosseous vascular canal in the lateral wall of the maxillary antrum

Shishir Ram Shetty,Saad Wahby Al Bayatti,Hesham Marei,Raghavendra Shetty,Hossam Abdelatty Abdelmagyd,Alexander Maniangat Luke 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.1

Objectives: The purpose of the study was to assess the occurrence, location, and dimensions of the intraosseous vascular canal in the lateral wall of the maxillary antrum using cone-beam computed tomography (CBCT). Materials and Methods: In this retrospective study, we examined 400 CBCT scans from our archive of patients who had earlier reported to a dental teaching hospital in the United Arab Emirates. The prevalence, location, and dimensions of the lateral antral intraosseous canal (LAIC) in the maxillary antrum were evaluated by 2 examiners using standardised methods. A third examiner was consulted in cases of disagreement. Results: The prevalence of LAIC was 62.3% (249 maxillary antra) among the study population. The mean distance between the most inferior point of the alveolar bone and the inferior border of the LAIC in the posterior maxillary region was 19.83±3.12 mm. There was a significant difference (P=0.05) between the maxillary molar and premolar regions in mean distance from the most inferior point of the alveolar bone and the inferior border of the LAIC. There was no statistically significant difference in mean distance between the most inferior point of the alveolar bone and the inferior border of the LAIC between dentulous and edentulous areas (P=0.1). The G3-intrasinusal type canal less than 1mm in diameter was the most common type of LAIC. Conclusion: This study established the approximate location of the LAIC in a United Arab Emirates cohort, which will assist the oral surgeon in selecting the appropriate site for sinus lift procedures with reduced risk of surgical hemorrhage.

Efficacy of Acupuncture in the Management of Primary Dysmenorrhea: A Randomized Controlled Trial

Geetha B. Shetty,Balakrishna Shetty,A. Mooventhan 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.4

Introduction: Dysmenorrhea constitutes one of the most frequent disorders in women of a fertile age. The present study was conducted to evaluate the efficacy of acupuncture in the management of primary dysmenorrhea. Materials and methods: Sixty females aged 17e23 years were randomly assigned to either a study group or a control group. The study group received acupuncture for the duration of 20 minutes/day, for 15 days/month, for the period of 90 days. The control group did not receive acupuncture for the same period. Baseline, during, and post assessments of both the groups were taken on day 1; day 30 and day 60; and day 90, respectively. Statistical analysis was performed by repeated measures of analysis of variance followed by post hoc analysis with Bonferroni adjustment for multiple comparisons, independent samples t test for visual analog scale score, and ManneWhitney U test for rest of the variables using statistical package for the social sciences, version 16. Results: This study showed a significant reduction in all the variables such as the visual analog scale score for pain, menstrual cramps, headache, dizziness, diarrhea, faint, mood changes, tiredness, nausea, and vomiting in the study group compared with those in the control group. Conclusion: Acupuncture could be considered as an effective treatment modality for the management of primary dysmenorrhea.

$^{68}Ga$-Labeled Radiopharmaceuticals for Positron Emission Tomography

Shetty, Dinesh,Lee, Yun-Sang,Jeong, Jae-Min The Korea Society of Nuclear Medicine 2010 핵의학 분자영상 Vol.44 No.4

[ $^{68}Ga$ ]is a promising emerging radionuclide for positron emission tomography (PET). It is produced using a $^{68}Ga/^{68}Ga$-generator, and thus, would enable the cyclotronindependent distribution of PET. However, new $^{68}Ga$-labeled radiopharmaceuticals that can replace $^{18}F$-labeled agents like [$^18F$]fluorodeoxyglucose (FDG) are needed. Most of the $^{68}Ga$-labeled derivatives currently used are peptide agents, but the developments of other agents, such as amino acid derivatives, nitroimidazole derivatives, and glycosylated human serum albumin, are being actively pursued in many laboratories. Thus, appearance of new $^{68}Ga$-labeled radiopharmaceuticals with high impact are expected in the near future. Here, we present an overview of $^{68}Ga$-labeled agents in terms of their clinical significances and relevances to the management of certain tumors, and pertinent pre-clinical developments.

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

Shetty, Dinesh,Khedkar, Jayshree K.,Park, Kyeng Min,Kim, Kimoon The Royal Society of Chemistry 2015 Chemical Society reviews Vol.44 No.23

<P>The design of synthetic, monovalent host-guest molecular recognition pairs is still challenging and of particular interest to inquire into the limits of the affinity that can be achieved with designed systems. In this regard, cucurbit[7]uril (CB[7]), an important member of the host family cucurbit[n]uril (CB[n], n = 5-8, 10, 14), has attracted much attention because of its ability to form ultra-stable complexes with multiple guests. The strong hydrophobic effect between the host cavity and guests, ion-dipole and dipole-dipole interactions of guests with CB portals helps in cooperative and multiple noncovalent interactions that are essential for realizing such strong complexations. These highly selective, strong yet dynamic interactions can be exploited in many applications including affinity chromatography, biomolecule immobilization, protein isolation, biological catalysis, and sensor technologies. In this review, we summarize the progress in the development of high affinity guests for CB[7], factors affecting the stability of complexes, theoretical insights, and the utility of these high affinity pairs in different challenging applications.</P>

Flexural bond strength behaviour in OPC concrete of NBS beam for various corrosion levels

Shetty, Akshatha,Venkataramanaa, Katta,Babu Narayan, K.S. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.49 No.1

Corrosion is one of the primary reasons why structures have limited durability. The present investigation is carried out to study the behavior of RC (Reinforced Concrete) structural members subjected to corrosion. Experimental investigations were carried out on National Bureau of Standard (NBS), RC beam specimens made of Ordinary Portland Cement (OPC) concrete. Load versus deflection behaviour was studied for different levels of corrosion i.e., 2.5%, 5%, 7.5% and 10%. It is observed that for every percentage increase in corrosion level, there is about 1.6% decrease in load carrying capacity. Also as the amount of corrosion increases there is a reduction in bond stress.

Posterior Stabilization of Unstable Sacral Fractures: A Single-Center Experience of Percutaneous Sacroiliac Screw and Lumbopelvic Fixation in 67 Cases

Shetty Ajoy Prasad,Renjith Karukayil Ramakrishnan,Perumal Ramesh,Anand Sri Vijay,Kanna Rishi Mugesh,Rajasekaran Shanmuganathan 대한척추외과학회 2021 Asian Spine Journal Vol.15 No.5

Study Design: This is a retrospective study. Purpose: Recent advances in intraoperative imaging and closed reduction techniques have led to a shifting trend toward surgical management in every unstable sacral fracture. This study aimed to evaluate the clinicoradiological outcome of the sacroiliac (SI) screw and lumbopelvic fixation (LPF) techniques and thereby delineate the indications for each. Overview of Literature: Optimal management guidelines for unstable sacral fractures are still lacking probably due to the rarity of these injuries and varying fixation trends. Methods: Out of the 67 patients, 40 and 27 were in the SI and LPF groups, respectively. The electronic medical record for each patient was reviewed, including patient demographic data, mode of trauma, coexisting injuries, neurological status (Gibbon’s four-grade system), Injury Severity Score, time from admission to operative stabilization, type of surgical stabilization, complications, return to the operating room, and treatment outcome measures using Majeed’s functional grading system and Matta’s radiological criteria. The minimum follow-up period was 2 years. Results: Noncomminuted longitudinal injuries with normal neurology and acceptable closed reduction have undergone SI screw fixation (n=40). Irreducible, comminuted, or high transverse fractures associated with dysmorphic anatomy or neurodeficit were managed by LPF (n=27). Excellent and good Majeed and Matta scores at 86.57% and 92.54% of the patients, respectively, were postoperatively achieved. Conclusions: Unstable sacral fractures can be effectively managed with percutaneous SI screw including vertically unstable injuries by paying strict attention to preoperative patient selection whereas LPF can be reserved for comminuted fractures, unacceptable closed reduction, associated neurodeficit, lumbosacral dysmorphism, and high transverse fractures.

Formation and Characterization of Gallium(III) Complexes with Monoamide Derivatives of 1,4,7‐Triazacyclononane‐1,4,7‐triacetic Acid: A Study of the Dependency of Structure on Reaction pH

Shetty, Dinesh,Choi, Soo Young,Jeong, Jae Min,Hoigebazar, Lathika,Lee, Yun‐,Sang,Lee, Dong Soo,Chung, June‐,Key,Lee, Myung Chul,Chung, Young Keun WILEY‐VCH Verlag 2010 European journal of inorganic chemistry Vol.2010 No.34

<P><B>Abstract</B></P><P>Two monoamide derivatives of 1,4,7‐triazacyclononane‐1,4,7‐triacetic acid (NOTA) conjugated with methylamine (<B>4</B>) or benzylamine (<B>5</B>) were synthesized by treating di‐<I>tert</I>‐butyl 1,4,7‐triazacyclononane‐1,4‐diacetate (<B>1</B>) with 2‐chloro‐<I>N</I>‐benzyl‐ or ‐<I>N</I>‐methylacetamide, followed by an acid cleavage reaction. Complexes of <B>4</B> and <B>5</B> chelated to Ga<SUP>3+</SUP> to give Ga‐<B>4</B> and Ga‐<B>5</B>, respectively, in reaction solutions at different pH values (3 and 5). Complexes Ga‐<B>4</B> and Ga‐<B>5</B> were characterized by single‐crystal X‐ray diffraction and multinuclear NMR spectroscopy. In the solid state, these complexes were isostructural, and the coordination spheres of the metal ions exhibited distorted octahedral geometries. In the case of the Ga‐<B>4</B> complex, which was formed at both pH values, the metal ion is coordinated to the amide nitrogen atom of the modified pendent arm of <B>4</B>. However, in the case of Ga‐<B>5</B>, the metal ion is coordinated to a nitrogen or an oxygen atom of the amide linkage when the pH of the reaction soultion was 5 or 3, respectively. No significant difference was found between the <SUP>1</SUP>H NMR spectra of the complexes formed at pH = 3 and 5. However, <SUP>71</SUP>Ga NMR spectra showed a broad resonance signal and a narrow singlet for the complex synthesized at the lower pH, but only a single narrow singlet for the complex prepared at neutral pH. Variable‐temperature <SUP>1</SUP>H NMR spectra showed that complexes Ga‐<B>4</B> and Ga‐<B>5</B> are rigid in solution. The stability of these complexes in the physiological pH range and at high temperature suggests that NOTA can be used as a bifunctional chelating agent to label biomolecules with radioactive gallium by direct conjugation of the complex to target molecules. This finding could open up a wide range of applications for NOTA‐type bifunctional chelating agents by eliminating the multi‐step synthesis routes required to introduce extra linker groups to the labelling agent.</P>

Scalp Acupuncture on the Immediate and Prolonged Motor Recovery in Spinal Cord Injury: a Case Report

Shetty Geetha B.,H L Nanjeshgowda 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.5

Spinal cord injury (SCI) is one of the main causes of lifelong motor impairment and is associated with important secondary complications. Thus, multifaceted treatments are needed for early functional recovery. Currently, in cases of SCI, surgery, stem cell treatment, medication, and physical therapy are used to repair and restore neuronal activity. Additionally, encouraging results have been reported on the use of acupuncture to modulate neuronal plasticity. Here, we present an SCI case involving a burst fracture at the L3 level, which was treated for 21 days using scalp acupuncture with residential physical therapy. Activation of the motor area was observed after the 1st day of treatment, with the patient completely regaining power and range of motion in the knees, Additionally, over 21 days, the patient exhibited markedly improved motor recovery and functional outcomes, which had not been observed over the previous six months. This report highlights the importance of complementing scalp acupuncture with intensive physical therapy for better motor recovery.

Core-Modified Analogues of Expanded Porphyrins Containing Rigid β,β′-Linked<i>o</i>-Phenylene Bridges

Shetti, Vijayendra S.,Kee, Se-Young,Lee, Chang-Hee Wiley (John WileySons) 2014 Chemistry - An Asian Journal Vol.9 No.3

<P>Structural modifications that lead to the creation of π-extended aromatic macrocyles involving a heterocyclic ring other than pyrrole and rigid β-β' linkages have not been well studied up to date. The rigidity caused by the conformational restriction would change the spectroscopic properties of the system as compared with those of the normal congeners. With these considerations, we have synthesized and fully characterized π-extended, core modified expanded porphyrins bearing rigid bipyrrole units. Core-modified naphthorubyrins were synthesized by the Lewis acid-catalyzed condensation of naphthobipyrrole with thiophene/furan diols, whereas naphthosapphyrins were obtained by reacting 2,9-diformyl-naphthobipyrrole with 16-thia/oxatripyrranes under mild reaction conditions. The core-modified analogues of both naphthorubyrin and naphthosapphyrin displayed the aromatic character. The dithiarubyrin analogues showed a lack of conformational change as expected and displayed well-resolved (1) H?NMR resonances at room temperature. On the other hand, the oxasapphyrin analogue adopts a furan-inverted geometry, and the ring inversion is independent of the protonation state. The oxanaphthosapphyrin also exhibited a weak fluorescence emission at 613?nm.</P>