담도폐쇄증 환아의 치과치료 : 증례보고

김재곤,양연미,백병주,이승익 大韓小兒齒科學會 2000 大韓小兒齒科學會誌 Vol.27 No.1

Intra-& extra-hepatic duct system의 경화를 야기하는 Biliary atresia는 출생시 1:10,000의 발생빈도를 보이며, 5년 이하의 생존율을 나타내는 예후가 좋지 않은 질환이다. 담도폐쇄증의 원인은 아직 명확하지 않으나, 유전적인 요소나 발육성 원인으로 믿고 있다. 임상적 특징으로 두드러진 황달, 간비증대, 가려움증, 지방변, 황색종, 성장지연, 문맥압항진증, 출혈, 골연하증, 복수 그리고 호흡기계 감염 등을 나타낸다. 구강내 소견으로 녹색을 띄는 변색치아, 유치의 확장된 치수강과 치근관, 치아맹출지연, 법랑질 형성부전, 그리고 심한 치아 우식 등이다. 담도폐쇄증은 조기진단과 수술에 의해 사망률이 감소되고, 성장과 발달이 정상으로 돌아오며 생존율도 향상되었으나 여전히 그 원인과 치료에 대한 연구가 계속되어야 할 것이다. 이에 저자는 전북대학교 치과병원에 내원한 2명의 담도폐쇄증환아에 대해 임상, 방사선학적 특징 및 적합한 처치에 대하여 보고하고자 한다. Congenital biliary atresia with pregressive sclerosis of the intra-and extra-hepatic duct system occurs in 1:10,000 live births, and has a poor prognosis with an expected survival of less than 5 years. Etiology of biliary atresia is unclear, however, it is believed a genetic or developmental cause. The clinical characteristics include pronounced jaundice, hepatosplenomegaly, pruritus, steatorrhea, xanthomas, growth retardation, portal hypetension, bleedings, ascites and respiratory infections. Oral manifestations have seldom been reported in patients with biliary atresia, but there may be enamel hypoplasia, delayed tooth eruption, and green teeth. Early diagnosis and surgical intervention have decreased morbidity, returned growth and development to normal and improved the prognosis for survival. Authors report the clinical and radiologic characteristics, proper managements about two cases with biliary atresia.

익산시 주변 하천 담수조류군집의 다양성에 대하여

최환석,김미연,김백호,최민규,박승택 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

혈관내초음파로 평가한 스텐트를 삽입한관동맥의 형태학적 특징

정남식,홍범기,임세중,백성일,이문형,장양수,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

ERP investigation of attentional disengagement from suicide-relevant information in patients with major depressive disorder

Baik, Seung Yeon,Jeong, Minkyung,Kim, Hyang Sook,Lee, Seung-Hwan Elsevier 2018 Journal of affective disorders Vol.225 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Previous studies suggest the presence of attentional bias towards suicide-relevant information in suicidal individuals. However, the findings are limited by their reliance on behavioral measures. This study investigates the role of difficulty in disengaging attention from suicide-relevant stimuli using the P300 component of event-related potentials (ERPs).</P> <P><B>Methods</B></P> <P>Forty-four adults with Major Depressive Disorder (MDD) were administered the spatial cueing task using suicide-relevant and negatively-valenced words as cue stimuli. Disengagement difficulty was measured using reaction time and P300 during invalid trials.</P> <P><B>Results</B></P> <P>P300 amplitudes at Pz were higher in suicide-relevant compared to negatively-valenced word condition on invalid trials for participants with low rates of suicidal behavior. However, no such difference was found among participants with high rates of suicidal behavior. P300 amplitudes for suicide-relevant word condition were negatively correlated with “lifetime suicide ideation and attempt” at Pz. No significant results were found for the reaction time data, indicating that the ERP may be more sensitive in capturing the attentional disengagement effect.</P> <P><B>Limitations</B></P> <P>The groups were divided according to Suicidal Behaviors Questionnaire-Revised (SBQ-R) total score. Neutral stimulus was not included as cue stimuli. Most participants were under medication during the experiment.</P> <P><B>Conclusions</B></P> <P>Our results indicate that patients with MDD and low rates of suicidal behavior show difficulty in disengaging attention from suicide-relevant stimuli. We suggest that suicide-specific disengagement difficulties may be related to recentness of suicide attempt and that acquired capability for suicide may contribute to reduced disengagement difficulties.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Reaction time and P300 amplitudes are higher for invalid trials than valid trials. </LI> <LI> Suicide-related attentional bias is associated with disengagement difficulties. </LI> <LI> Suicidal patients show reduced difficulty disengaging attention from suicidal words. </LI> <LI> Greater lifetime suicidal behavior is related to less disengagement difficulties. </LI> </UL> </P>

전자간호기록을 이용한 후향적 응급실 간호업무 분석

백승연(Baik, Seung Yeon),조은희(Cho, Eunhee),김영아(Kim, Young Ah),최모나(Choi, Mona) 한국성인간호학회 2019 성인간호학회지 Vol.31 No.5

A LuxR-type Transcriptional Regulator, PsyR, Coordinates Regulation of Pathogenesis-related Genes in Pseudomonas syringae pv. tabaci

Yeon Hee Choi(최연희),Jun Seung Lee(이준승),Sora Yun(윤소라),Hyung Suk Baik(백형석) 한국생명과학회 2015 생명과학회지 Vol.25 No.2

Pseudomonas syringae pv. tabaci 11528은 담배를 숙주로 하여 wildfire disease를 일으키는 식물 병원성 세균이다. P. syringae pv. tabaci psyR deletion mutant를 이용하여 swarming motility, tabtoxin 생산능, siderophore 생산능, AHL 생산능 등의 phenotypic test를 수행하였다. psyR deletion mutant는 wild-type 균주보다 swarming motility가 증가하였고, tabtoxin 생산 또한 증가하였다. 하지만 siderophore와 AHL 생산능은 감소하였고 virulence 또한 지연되었다. 이러한 결과로 PsyR이 QS regulator로 작용한다는 사실과 더불어 병원성 유전자의 조절에도관여한다는 것을 확인하였다. PsyR이 각각의 병원성 유전자의 발현을 조절하는 regulator들에게 미치는 영향을 전사단계에서 확인하기 위해 fur, gacA, psyI, prhI, prhA, hrpR, hrpA 유전자들을 정량적 real-time PCR (qRT-PCR)방법으로 확인하였다. 또한 PsyR에 의한 병원성 유전자 조절이 DNA상에 직접적으로 결합하여 일어나는 것인지 아니면 다른 경로를 통해 간접적으로 일어나는 것인지를 확인할 필요가 있어 정제한 PsyR 단백질과 병원성 관련 유전자들의 upstream region 서열을 이용하여 electrophoretic mobility shift assay (EMSA)를 수행한 결과 본 연구에서 선정한 병원성 관련 유전자들이 PsyR에 의해 직접적으로 조절되지는 않는다는 사실을 밝혔다. Pseudomonas syringae pathovar tabaci is a plant pathogenic bacterium that causes wildfire disease in tobacco plants. In P. syringae pv. tabaci, PsyI, a LuxI-type protein, acts as an AHL synthase, while primary and secondary sequence analysis of PsyR has revealed that it is a homolog of the LuxR-type transcriptional regulator that responds to AHL molecules. In this study, using phenotypic and genetic analyses in P. syringae pv. tabaci, we show the effect of PsyR protein as a quorum-sensing (QS) transcriptional regulator. Regulatory effects of PsyR on swarming motility and production of siderophores, tabtoxin, and N-acyl homoserine lactones were examined via phenotypic assays, and confirmed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Further qRT-PCR showed that PsyR regulates expression of these virulence genes in response to environmental signals. However, an upstream region of the gene was not bound with purified MBP-PsyR protein; rather, PsyR was only able to shift the upstream region of psyI. These results suggested that PsyR may be indirectly controlled via intermediate-regulatory systems and that auto-regulation by PsyR does not occur.

Local deformation of liquid crystal director induced by translational motion of carbon nanotubes under in-plane field

Baik, In-Su,Jeon, Sang Yeon,Jeong, Seok Jin,Lee, Seung Hee,An, Kay Hyeok,Jeong, Seok Ho,Lee, Young Hee American Institute of Physics 2006 JOURNAL OF APPLIED PHYSICS - Vol.100 No.7

Isolation of Bacteria with Protease Activity from Cheonggukjang and Purification of Fibrinolytic Enzyme

Yeon Hee Choi(최연희),Jun Seung Lee(이준승),So Young Bae(배소영),Keun Jae Yang(양근재),Kyu Won Yeom(염규원),Dong Hyeok Jo(조동혁),Ock hwa Kang(강옥화),Hyung Suk Baik(백형석) 한국생명과학회 2013 생명과학회지 Vol.23 No.2

혈전용해효소를 생산하는 균주 분리를 위해서 우선 한국, 일본 등지에서 모은 21개의 청국장 시료를 준비하였고 총 268개의 균주를 분리하였다. 이 중에서 1% skim milk가 포함된 nutrient agar 배지에서 protease를 생산하는 bacteria를 분리하였고, 이 결과로 22개의 균주가 분리되었다. 균주들은 apiweb을 통해 생화학적 특성에 근거하여 동정하였다. 또한 세균동정을 위해 16S rRNA 염기서열 분석을 수행하였다. 분리된 대부분의 균주는 Bacillus subtilis와 Bacillus amyloliquefaciens였다. 혈전용해효소의 활성은 fibrin plate 방법에 의해 측정되었고 A2-14, A2-20, C1-05, C1-09, F2-01로 명명된 다섯 균주가 선택되었다. 이중에서 A2-20 균주는 강한 혈전용해 활성을 보였고 동정결과 Bacillus amyloliquefaciens에 가까웠다. A2-20 균주에 의해 생산되는 혈전용해효소는 균 상등액을 이용한 gel filtration과 ion exchange chromatography를 거쳐 부분정제 되었다. 부분 정제된 효소의 최적 pH는 7.0이었고, 최적 온도는 35℃였다. 정제된 단백질의 분석은 SDS-PAGE와 zymography로 이루어졌다. 이와 더불어 혈전용해효소의 유전자적 분석도 수행되었으며 A2-20 균주가 생산하는 혈전용해효소의 부분적인 염기서열과 유전적 상동성을 보이는 서열을 규명하였다. To isolate the fibrinolytic enzyme, 268 strains from 21 samples were morphologically isolated from Cheonggukjang collected from Korea and Japan. Among the 268 strains, protease-producing bacteria were isolated in nutrient agar medium including 1% skimmed milk. As a result of this, 22 strains were isolated. Apiweb site was used to identify these strains based on their biochemical properties. In addition, 16S rRNA sequencing was performed to identify the strain. Most of the identified strains were Bacillus subtilis and B. amyloliquefaciens. Fibrinolytic enzyme activity was measured with the fibrin plate method. Five strains were finally selected: A2-14, A2-20, C1-05, C1-09, and F2-01. Of those five strains, the A2-20 strain, which is close to B. amyloliquefaciens, showed the strongest fibrinolytic activity. The fibrinolytic enzyme produced by the A2-20 strain was partially purified from culture supernatant by gel filtration and ion exchange chromatography. The optimal pH and temperature values of the partially purified enzyme were 7.0 and 35°C, respectively. Purified protein analysis was carried out with SDS-PAGE and zymography. A genetic analysis was also conducted by PCR based on the consensus sequence of fibrinolytic enzyme. Corresponding genes with a partial sequence of the A2-20 strain were identified.

송전선로 점퍼선 횡진방지용 지지애자장치 개발

백승도(Seung-Do Baik),민병욱(Byeong-Wook Min),위화복(Hwa-Bog Wi),박기용(Kee-Yong Park),금의연(Eui-Yeon Keum),정헌수(Heon-Soo Jeong) 대한전기학회 2005 대한전기학회 학술대회 논문집 Vol.2005 No.7

Evaluation of PCR-Based Assay for Diagnosis of Spotted Fever Group Rickettsiosis in Human Serum Samples

Choi, Yeon-Joo,Lee, Seung-Hyun,Park, Kyung-Hee,Koh, Young-Sang,Lee, Keun-Hwa,Baik, Hyung-Suk,Choi, Myung-Sik,Kim, Ik-Sang,Jang, Won-Jong AMERICAN SOCIETY FOR MICROBIOLOGY 2005 CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY Vol.12 No.6

<B>ABSTRACT</B><P>A nested PCR assay was developed for the detection of spotted fever group (SFG) rickettsiae in serum samples. The assay was based on specific primers derived from the rickettsial outer membrane protein B gene (<I>rompB</I>) of <I>Rickettsia conorii</I>. An SFG rickettsia-specific signal is obtained from <I>R. akari</I>, <I>R. japonica</I>, <I>R. sibirica</I>, and <I>R. conorii</I>. Other bacterial species tested did not generate any signal, attesting to the specificity of the assay. As few as seven copies of the <I>rompB</I> gene of <I>R. conorii</I> could be detected in 200 μl of serum sample. The assay was evaluated with a panel of sera obtained from patients with acute-phase febrile disease tested by immunofluorescent antibody assay (IFA). The SFG rickettsia-specific DNA fragment was detected in 71 out of 100 sera, which were proven to have immunoglobulin M antibodies against SFG rickettsial antigen by IFA. The results were further confirmed by restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated that this PCR assay is suitable for the diagnosis of spotted fever group rickettsiosis in Korea.</P>