Anti Tumoral Properties of Punica granatum (Pomegranate) Seed Extract in Different Human Cancer Cells

Seidi, Khaled,Jahanban-Esfahlan, Rana,Abasi, Mozhgan,Abbasi, Mehran Mesgari Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: Punica granatum (PG) has been demonstrated to possess antitumor effects on various types of cancer cells. In this study, we determined antiproliferative properties of a seed extract of PG (PSE) from Iran in different human cancer cells. Materials and Methods: A methanolic extract of pomegranate seeds was prepared. Total phenolic content (TPC) and total flavonoid content (TFC) were assessed by colorimetric assays. Antioxidant activity was determined with reference to DPPH radical scavenging activity. The cytotoxicity of different doses of PSE (0, 5, 20, 100, 250, 500, $1000{\mu}g/ml$) was evaluated by MTT assays with A549 (lung non small cell carcinoma), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer cells), and PC-3 (prostate adenocarcinoma) cells. Results: Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison to negative controls at all tested doses ($5-1000{\mu}g/ml$). In all studied cancer cells, PSE reduced the cell viability to values below 23%, even at the lowest doses. In all cases, IC50 was determined at doses below $5{\mu}g/ml$. In this regard, SKOV3 ovarian cancer cells were the most responsive to antiproliferative effects of PSE with a maximum mean growth inhibition of 86.8% vs. 82.8%, 81.4% and 80.0% in MCF-7, PC-3 and A549 cells, respectively. Conclusions: Low doses of PSE exert potent antiproliferative effects on different human cancer cells SKOV3 ovarian cancer cells as most and A549 cells ar least responsive regarding cytotoxic effects. However, the mechanisms of action need to be addressed.

The Balancing of Disassembly Line of Automobile Engine Using Genetic Algorithm (GA) in Fuzzy Environment

Seidi, Masoud,Saghari, Saeed Korean Institute of Industrial Engineers 2016 Industrial Engineeering & Management Systems Vol.15 No.4

Disassembly is one of the important activities in treating with the product at the End of Life time (EOL). Disassembly is defined as a systematic technique in dividing the products into its constituent elements, segments, sub-assemblies, and other groups. We concern with a Fuzzy Disassembly Line Balancing Problem (FDLBP) with multiple objectives in this article that it needs to allocation of disassembly tasks to the ordered group of disassembly Work Stations. Tasks-processing times are fuzzy numbers with triangular membership functions. Four objectives are acquired that include: (1) Minimization of number of disassembly work stations; (2) Minimization of sum of idle time periods from all work stations by ensuring from similar idle time at any work-station; (3) Maximization of preference in removal the hazardous parts at the shortest possible time; and (4) Maximization of preference in removal the high-demand parts before low-demand parts. This suggested model was initially solved by GAMS software and then using Genetic Algorithm (GA) in MATLAB software. This model has been utilized to balance automotive engine disassembly line in fuzzy environment. The fuzzy results derived from two software programs have been compared by ranking technique using mean and fuzzy dispersion with each other. The result of this comparison shows that genetic algorithm and solving it by MATLAB may be assumed as an efficient solution and effective algorithm to solve FDLBP in terms of quality of solution and determination of optimal sequence.

The Balancing of Disassembly Line of Automobile Engine Using Genetic Algorithm (GA) in Fuzzy Environment

Masoud Seidi,Saeed Saghari 대한산업공학회 2016 Industrial Engineeering & Management Systems Vol.15 No.4

Disassembly is one of the important activities in treating with the product at the End of Life time (EOL). Disassembly is defined as a systematic technique in dividing the products into its constituent elements, segments, sub-assemblies, and other groups. We concern with a Fuzzy Disassembly Line Balancing Problem (FDLBP) with multiple objectives in this article that it needs to allocation of disassembly tasks to the ordered group of disassembly Work Stations. Tasks-processing times are fuzzy numbers with triangular membership functions. Four objectives are acquired that include: (1) Minimization of number of disassembly work stations; (2) Minimization of sum of idle time periods from all work stations by ensuring from similar idle time at any work- station; (3) Maximization of preference in removal the hazardous parts at the shortest possible time; and (4) Maximization of preference in removal the high-demand parts before low-demand parts. This suggested model was initially solved by GAMS software and then using Genetic Algorithm (GA) in MATLAB software. This model has been utilized to balance automotive engine disassembly line in fuzzy environment. The fuzzy results derived from two software programs have been compared by ranking technique using mean and fuzzy dispersion with each other. The result of this comparison shows that genetic algorithm and solving it by MATLAB may be assumed as an efficient solution and effective algorithm to solve FDLBP in terms of quality of solution and determination of optimal sequence.

Synthesis and Characterization of a New Amino Chitosan Derivative for Facilitated Transport of CO2 Through Thin Film Composite Membranes

Farzad Seidi,Shahab Saedi,Shadi Haghdust,Xu Xiang 한국고분자학회 2016 Macromolecular Research Vol.24 No.1

Industrial polymeric membranes suffer from low CO2 permeability as well as low gas pair selectivity. The presence of a suitable carrier in polymer matrix that can react reversibly with CO2 can enhance the CO2 permeability and CO2-selective properties of polymeric membranes. The abundant amino groups of chitosan make it a good candidate for a CO2 carrier. In this research, a new water-soluble amino derivative of chitosan was synthesized and characterized using FTIR, 1H NMR, and elemntal analysis. The synthesised chitosan derivative was blended with polyvinyl alchol and used to prepare thin film composite (TFC) membranes for facilitated transport of CO2 from a CO2/CH4 gas mixture. The effect of feed pressure, feed temperature and chitosan derivative content on the CO2 permeance, CH4 permeance and the CO2/CH4 selectivtiy of the prepared membranes were investigated. The new TFC membranes possessed acceptable CO2 permeance and CO2/CH4 selectivity.

Novel DOX-MTX Nanoparticles Improve Oral SCC Clinical Outcome by Down Regulation of Lymph Dissemination Factor VEGF-C Expression in vivo: Oral and IV Modalities

Abbasi, Mehran Mesgari,Monfaredan, Amir,Hamishehkar, Hamed,Seidi, Khaled,Jahanban-Esfahlan, Rana Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because of its high propensity for local invasion and cervical lymph node dissemination. The aim of present study was to evaluate the efficacy of novel pH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in terms of their potential to change the VEGF-C expression profile in a rat OSCC model. Materials and Methods: 120 male rats were divided into 8 groups of 15 animals administrated with 4-nitroquinoline-1-oxide to induce OSCCs. Newly formulated doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) and free doxorubicin were IV and orally administered. Results: Results indicated that both oral and IV forms of DOX-MTX-nanoparticle complexes caused significant decrease in the mRNA level of VEGF-C compared to untreated cancerous rats (p<0.05). Surprisingly, the VEGF-C mRNA was not affected by free DOX in both IV and oral modalities (p>0.05). Furthermore, in DOX-MTX NP treated group, less tumors characterized with advanced stage and VEGF-C mRNA level paralleled with improved clinical outcome (p<0.05). In addition, compared to untreated healthy rats, the VEGF-C expression was not affected in healthy groups that were treated with IV and oral dosages of nanodrug (p>0.05). Conclusions: VEGF-C is one of the main prognosticators for lymph node metastasis in OSCC. Down-regulation of this lymph-angiogenesis promoting factor is a new feature acquired in group treated with dual action DOX-MTX-NPs. Beside the synergic apoptotic properties of concomitant use of DOX and MTX on OSCC, DOX-MTX NPs possessed anti-angiogenesis properties which was related to the improved clinical outcome in treated rats. Taking together, we conclude that our multifunctional doxorubicin-methotrexate complex exerts specific potent apoptotic and anti-angiogenesis properties that could ameliorate the clinical outcome presumably via down-regulating dissemination factor-VEGF-C expression in a rat OSCC model.

Properties of Different Types of Rough Approximations Defined by a Family of Dominance Relations

A. K. Salah,Essam El-Seidy,A.S Salama 한국지능시스템학회 2022 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.22 No.2

In this study, we examine the properties of three types of lower and upper approximations ofa non-empty finite set based on the dominance class generated by the dominance relations. We generalize these types using a family of dominance relations {Ri: i = 1, 2, . . . , n} andstudying their properties

A Randomized Open-Label Trial to Assess the Effect of Plant Sterols Associated with Ezetimibe in Low-Density Lipoprotein Levels in Patients with Coronary Artery Disease on Statin Therapy

Gisane Biacchi Gomes,Ana Denise Zazula,Leonardo Seidi Shigueoka,Rosangela Alquieri Fedato,Ana Beatriz Brenner Affonso da Costa,Luiz Cesar Guarita-Souza,Cristina Pellegrino Baena,Marcia Olandoski,Jose´ 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.1

Consumption of food products enriched with plant sterols and the use of ezetimibe reduce cholesterol absorption in the intestine and effectively reduce low-density lipoprotein (LDL) plasma levels. We evaluated the therapeutic effect of the ezetimibe+plant sterol association in patients with coronary artery disease still not reaching recommended lipid levels despite the use of statins. We performed a prospective open-label study with 41 patients with stable coronary disease and LDL >70 mg/dL. Patients were randomized into four groups for a 6-week treatment: the control (CT) group remained on the same statin therapy, the ezetimibe (EZ) group received 10 mg/day of ezetimibe, the plant sterol (PS) group received spread enriched with 2 g of plant sterols, and the ezetimibe+PS (EZ+PS) group received 10 mg/day EZ +2 g PS. Initial mean LDL level was 97.4 ± 31.1 mg/dL in control group, 105.1 ± 23.1 mg/dL in EZ group, 95.4 ± 27.7 mg/dL in PS group, and 97.0 ± 8.3 mg/dL in EZ+PS group (P > .05). After 6 weeks of treatment, LDL of patients slightly increased in the control group (+8.9%; P > .05) and dropped in EZ group (-19.1%; P = .06), PS group (-16.6%; P = .01), and EZ+PS group (-27.3%; P < .01). Mean LDL levels after treatment were 70.5 – 17.9 mg/dL in EZ+PS group, lower than the other groups (control was 106.1 ± 34.9 mg/dL, EZ group was 85.0 ± 35.6 mg/dL, and PS was 79.6 ± 29.7 mg/dL) (P = .05 variance analysis factor [ANOVA]). Body weight, body–mass index, and glucose plasma levels did not change significantly after intervention. The combination of PS+ezetimibe was associated with lower LDL levels and suggests beneficial therapeutic effect against major cardiovascular events.

Synthesis, Characterization and ESR Studies of New Copper(II) Complexes of Vicinal Oxime Ligands

El-Tabl, Abdou S.,Shakdofa, Mohamad M.E.,El-Seidy, Ahmed M.A. Korean Chemical Society 2011 대한화학회지 Vol.55 No.4

Ethoxylacetyl oxime ligands [HL, (1) and $H_2L^1$, (3)] react with copper(II) acetate monohydrate yield octahedral and square planar complexes, respectively. The complexes have been postulated due to elemental analyses, IR, UVVis. spectra, magnetic susceptibility, conductivity and ESR spectra. Molar conductance of the complexes in DMF indicates a non-ionic character. The ESR spectra of [$(L)_2Cu(H_2O)_2$], (2) complex at room temperature and 77K are characteristic of an axial symmetry ($d_{x2-y2}$) with covalent bond character and have a large line width typical of dipolar interactions. However, [$(L^1)Cu$], (4) complex in the solid state showed spectra of marked broadening and loss of hyperfine splitting confirming spinexchange interactions between the copper(II) sites. The spectrum of the doped copper(II) complex at room temperature showed super-hyperfine splitting from coordinated nitrogen atoms and it has an axial type ($d_{x2-y2}$) with covalent bond character and an essentially square-planar arrangement around the copper(II) ion. The spectrum of [$(L^1)Cu$], (4) in frozen methanol at 77K was characteristic of the triplet state of a dimer species and the distance found between the two copper(II) centers was calculated and is equal to 4.8 ${\AA}$. Ethoxylacetyl oxime 리간드 [HL, (1) 및 $H_2L^1$, (3)]와 아세트산 구리(II) 일수화물을 반응시켜 팔면체 및 사각평면착물을 각각 얻었다. 이들 착물을 원소분석, IR 및 UV-Vis 스펙트럼, 자기수자율, 전기전도도 및 ESR 스펙트럼으로 조사하였다. DMF에서 이들 착물의 몰전기전도도 측정한 결과 비이온성을 보였다. 실온 및 77 K에서 [$(L)_2Cu(H_2O)_2$], (2) 착물의 ESR 스펙트럼은 배위결합성을 갖는 축대칭($d_{x2-y2}$)의 특성을 보이며, 또한 전형적인 쌍극자 상호작용에 의해 큰 띠나비를 갖는다. 그러나 착물 [$(L^1)Cu$], (4)는 고체상태에서 큰 띠넓임으로 인해 초미세 갈라짐이 관찰되지 않았는데, 이는 구리(II) 결합자리들 사이의 스핀-교환 상호작용에 의한 것으로 확인되었다. 실온에서 도포된 구리(II) 착물의 스펙트럼은 배위된 질소원자로 인해 극초미세 갈라짐을 보였다. 또한 이 물질은 공유 결합성을 갖는 축형태($d_{x2-y2}$)의 특성을 보이고, 구리(II) 이온의 환경은 기본적으로 사각-평면 배열을 갖는다. 77 K의 얼어있는 메탄올에서 [$(L_1)Cu$], (4)의 스펙트럼은 이합체 화합물의 삼중항 상태의 특성을 보이며, 두 구리(II) 이온 중심 사이의 거리를 계산한 결과 4.8 이다.

Synthesis, Characterization and Biological Studies of New Mn(II), Ni(II), Co(II), Cu(II) and Zn(II) of 2-(benzothiazol-2-yl)-N'-(2,5-dihydroxybenzylidene)acetohydrazide

El-Tabl, Abdou S.,Shakdofa, Mohamad M.E.,El-Seidy, Ahmed M.A.,Al-Hakimi, Ahmed N. Korean Chemical Society 2011 대한화학회지 Vol.55 No.1

2-(Benzothiazol-2-yl)-N'-(2,5-dihydroxybenzylidene)acetohydrazide 에대한새로운일련의 Mn(II), Ni(II), Co(II), Cu(II) 및 Zn(II) 착물을 합성하여 그 특성을 원소분석, IR, UV-vis, $^1H$-NMR, 질량분석, ESR, 자기수자율 및 몰 전기전도도 측정에 의하여 조사하였다. 이들 착물의 기하구조가 사각평면 또는 팔면체임을 분광학적 데이터 및 자기적 측정으로부터 알았다. 이 리간드와 해당착물의 세균에(Aspergillus nigar 및 Fusarium oxysporium) 대한생물학적활성을 조사하였다. 그 결과, 금속착물들은 리간드 및 금속이온 모두에 비해 더 큰 활성을 나타내었다. New series of Mn(II), Ni(II), Co(II), Cu(II) and Zn(II) of the 2-(benzothiazol-2-yl)-N'-(2,5-dihydroxybenzylidene) acetohydrazide have been synthesized and characterized by elemental analysis, IR, UV-vis, $^1H$-NMR, mass and ESR spectra, magnetic susceptibility and molar conductivity measurements. The spectral data and magnetic measurements of the complexes indicate that, the geometries are either square planar or octahedral. The biological activity of the ligand and its complexes against fungi (Aspergillus nigar and Fusarium oxysporium) were investigated. The metal complexes exhibited higher activity than both the parent ligand and the corresponding metal ion.

Synthesis, Spectroscopic, and Biological Studies of Chromium(III), Manganese(II), Iron(III), Cobalt(II), Nickel(II), Copper(II), Ruthenium(III), and Zirconyl(II) Complexes of N¹,N²-Bis(3-((3-hydroxynaphthalen-2-yl)methylene-amino)pr

Al-Hakimi, Ahmed N.,Shakdofa, Mohamad M.E.,El-Seidy, Ahemd M.A.,El-Tabl, Abdou S. Korean Chemical Society 2011 대한화학회지 Vol.55 No.3

[ $N^1,N^2$ ]bis(3-((3-hydroxynaphthalen-2-yl)methylene-amino)propyl)phthalamide ($H_4L$, 1) 의 새로운 크롬(III), 망간(II), 철(III), 코발트(II), 니켈(II), 구리(II), 루테늄(III) 및 산화 지르코늄(II) 착물을 합성하여 원소분석, 물리적 성질 및 분광학적으로 특성을 규명하였다. 분광학적 결과를 통해 이 리간드는 $[H_4LMX_2(H_2O)]{\cdot}nH_2O$ (M = Cu(II), Ni(II), Co(II), X = Cl 또는 $NO_3$)의 일반식을 갖는 착물2-5에서는 중성의 삼배위 리간드로 행동한다. 또는 $[H_4L(ZrO)_2Cl_2]{\cdot}8H_2O$ 의 일반식을 갖는 착물 6-9 에서는 이염기성 육배위 리간드로 행동한다. DMF 용액에서의 몰전기전도도 실험결과 이들 착물은 비이온성을 나타낸다. 고체 구리착물 2, 5 및 6 의 ESR 스펙트럼에서 $g_{\parallel}$ >g> $g_e$을 보이는데, 이는 일그러진 팔면체구조와 큰 공유결합성을 갖는 $d{_x}^2{_{-y}}^2$ 오비탈에 비공유 전자쌍이 존재함을 의미한다. 이합체 구리(II) 착물 $[H_2LCu_2Cl_2(H_2O)_4]{\cdot}3H_2O$ (6)에 대해 두 구리원자 사이의 거리를 ESR 스펙트럼으로부터 추정한 parallel component 의 field zero splitting 파라메타를 이용하여 계산하였다. 이들 화합물의 항박테리아 및 항균 활성도를 측정한 결과, 몇가지 금속 착물의 경우 표준시약인 tetracycline (박테리아) 및 Amphotricene B (균류)보다 더 큰 저해효과를 보였다. Novel chromium(III), manganese(II), iron(III), cobalt(II), nickel(II), copper(II), ruthenium(III), and zirconyl(II) complexes of $N^1,N^2$-bis(3-((3-hydroxynaphthalen-2-yl)methylene-amino)propyl)phthalamide ($H_4L$, 1) have been synthesized and characterized by elemental, physical, and spectral analyses. The spectral data showed that the ligand behaves as either neutral tridentate ligand as in complexes 2-5 with the general formula $[H_4LMX_2(H_2O)]{\cdot}nH_2O$ (M=Cu(II), Ni(II), Co(II), X = Cl or $NO_3$), neutral hexadentate ligand as in complexes 10-12 with the general formula $[H_4LM_2Cl_6]{\cdot}nH_2O$ (M=Fe(III), Cr(III) or Ru(III)), or dibasic hexadentate ligand as in complexes 6-9 with the general formula $[H_2LM_2Cl_2(H_2O)_4]{\cdot}nH_2O$ (M = Cu(II), Ni(II), Co(II) or Mn(II), and 13 with general formula $[H_4L(ZrO)_2Cl_2]{\cdot}8H_2O$. Molar conductance in DMF solution indicated the non-ionic nature of the complexes. The ESR spectra of solid copper(II) complexes 2, 5, and 6 showed $g_{\parallel}$ >g> $g_e$, indicating distorted octahedral structure and the presence of the unpaired electron in the $N^1,N^2$ orbital with significant covalent bond character. For the dimeric copper(II) complex $[H_2LCu_2Cl_2(H_2O)_4]{\cdot}3H_2O$ (6), the distance between the two copper centers was calculated using field zero splitting parameter for the parallel component that was estimated from the ESR spectrum. The antibacterial and antifungal activities of the compounds showed that, some of metal complexes exhibited a greater inhibitory effect than standard drug as tetracycline (bacteria) and Amphotricene B (fungi).