A Study on an Independent Steering & Driving Control Algorithm for 6WS/6WD Vehicles

Joo-Young Choi,Dong-Hyung Kim,Chang-Jun Kim,Young-Ryul Kim,Sang-Ho Kim5,Chang-Soo Han 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Skid-steered vehicles are favored for military use in off-road operations because of their high maneuverability and mobility on extreme terrains and obstacles. There is a trend towards transforming steered tracked vehicles to skid-steered wheel vehicles for high speed at the expense of reduced mobility. Skid-steered vehicles turn by generating different longitudinal forces at the tires due to the application of different torques to the wheels on the opposite side of the vehicle. Conventional vehicles, however, cannot generate an opposite driving force at each side wheel. Using an independent steering and driving system, six-wheel vehicles can show better performance than conventional vehicles. Hybrid steering is a combination of skid steering in the load velocity and the steered wheel system at high speed. This steering enhances maneuverability under low speed and stability at high speed. This paper describes a 6WS/6WD vehicle for hybrid steering in three parts: the Vehicle Model, the Control Algorithm for Hybrid Steering, and a Simulation. First, the vehicle model is an application of the TruckSim software for 6WS and 6WD. Second, the hybrid steering control algorithm describes the optimum tire force distribution method for energy savings. The last is simulation and verification.

Construction of Stably Transformed Bm 5 Cells by Using Autographa californica Nuclear Polyhedrosis Virus IE 0 Gene

Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells

METHOD AND TECHNOLOGY : A Simple DNA Preparation Method for High Quality Polymerase Chain Reaction in Rice

( Sung Ryul Kim ),( Jungil Yang ),( Gynheung An ),( Kshirod K Jena ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.1

Preparation of DNA is cumbersome especially in the case of large numbers of plant samples. Several simple plant DNA preparation methods have been developed for use in conjunction with polymerase chain reaction (PCR) analysis. However, those methods have not been adopted widely for rice molecular analysis. We present a new, simple, and inexpensive method using tris-phosphate (TPE) ethylenediaminetetraacetic acid (EDTA) buffer (100 mM tris-HCl pH9.5, 1 M KCl, 10 mM EDTA pH 8.0) without phenol-chloroform extraction and DNA precipitation steps. The method consists of five steps: leaf tissue grinding, incubating in TPE buffer at 65oC for 20 to 90 minutes, diluting extracts with water, centrifuging to sediment tissue debris, and transferring the supernatant for direct use in PCR or storage. Agarose gel analysis of the crude extracts indicated that the method produced intact genomic DNA (gDNA) from young and old leaves of both young seedlings and mature plants. Leaf sample size (0.5 to 8.0 cm long) for DNA preparation was less sensitive to PCR than the previous methods. DNA quality was tested through PCR amplification of various GC content regions and product sizes, and we obtained bands from all samples, indicating that the method produced suitable DNA quality for PCR. gDNAs were stable for longer than eight months at 4oC. This protocol enabled one person to handle several hundred samples in a day and was tested through various PCR-gel analyses such as genotyping of rice T-DNA mutant lines, positional cloning of rice mutant, and high throughput marker-assisted breeding using allele-specific SNP/Indel markers.

Preparation and characterization of mesoporous Zr-WO_<i>x</i>/SiO₂ catalysts for the esterification of 1-butanol with acetic acid

Kim, Tae Yong,Park, Dae Sung,Choi, Youngbo,Baek, Jayeon,Park, Jae Ryul,Yi, Jongheop The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.19

<P>Zr-WO<SUB><I>x</I></SUB> clusters on WO<SUB><I>x</I></SUB>/ZrO<SUB>2</SUB> catalysts are known to be active sites for the acid catalyzed reactions, such as dehydration of alcohols and alkane isomerization reactions. However, synthetic methods for producing high density of Zr-WO<SUB><I>x</I></SUB> clusters with high surface areas are not currently available. Herein, a facile method for preparing mesoporous Zr-WO<SUB><I>x</I></SUB>/SiO<SUB>2</SUB> is proposed and the effect of Zr/W ratio on its structure and acidity was examined. Results showed that the sequential hydrolysis of zirconium and tungsten <I>via</I> soft-templating resulted in the formation of Zr-WO<SUB><I>x</I></SUB> clusters with uniform mesopore structures and a high acidity. The prepared Zr-WO<SUB><I>x</I></SUB>/SiO<SUB>2</SUB> was characterized by N<SUB>2</SUB> physisorption, XRD, TEM, XPS, UV-Vis spectroscopy, NH<SUB>3</SUB>-TPD and <I>in situ</I> FTIR. Catalytic performance for the esterification of 1-butanol with acetic acid was evaluated. The materials had a high surface area of over 500 m<SUP>2</SUP> g<SUP>−1</SUP> and ordered cylindrical pores with a uniform size of <I>ca.</I> 5 nm. Below a Zr/W ratio of ∼0.5, the zirconium was primarily associated with tungstate rather than SiO<SUB>2</SUB>, which indicates the formation of Zr-WO<SUB><I>x</I></SUB> clusters. The highest density of Zr-WO<SUB><I>x</I></SUB> clusters was obtained at a Zr/W ratio of 0.3 with a strong Brønsted acidity. Consequently, Zr-WO<SUB><I>x</I></SUB>/SiO<SUB>2</SUB>, as a Zr/W ratio of 0.3, exhibited the highest activity with a significantly improved performance compared to HZSM-5 and WO<SUB><I>x</I></SUB>/ZrO<SUB>2</SUB> catalysts.</P> <P>Graphic Abstract</P><P>Mesoporous Zr-WO<SUB><I>x</I></SUB>/SiO<SUB>2</SUB> catalysts with active Zr-WO<SUB><I>x</I></SUB> clusters were prepared and characterized. They exhibited significantly improved activity than conventional solid acids in the esterification of 1-butanol and acetic acid. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm30904a'> </P>

A Study on the General Characteristics and Instrumental Analysis of Natural Omija Extract

Sung, Ki-Chun,Kim, Ki-Jun,Kim, Yong-Ryul,Nam, Sang-Sung The Korean Society of Applied Science and Technolo 2013 한국응용과학기술학회지 Vol.30 No.2

Omija component was known to possess natural odor, taste, color, and various general characteristics. Omija extraction was extracted using ethanol as a solvent. Omija extract showed a red-purple color of some viscous liquid state. Some conclusions from natural Omija extract were obtained as follow. The result of antimicrobial experiment to add Omija extract, the number of microbial population showed negative reaction from 3 days after it cultivated. This phenomenon could confirm that Omija component affected to antimicrobial effect. The result of dyeing experiment to add Omija extract, fiber dyeing effect showed with some ivory color after dyed to cotton and silk. Also, this phenomenon could confirm that Omija component affected to natural dyeing effect from observated dye state with biological microscope(BM). The result of instrumental analysis, inorganic components of K(109.60ppm), Na(3.500ppm), Ca(1.205ppm), Mg(0.900ppm), Li(0.350ppm), Si(0.380ppm), Cu(0.250ppm), Fe(0.125ppm), Zn(0.090ppm), etcs from Omija were ascertained with ICP/OES, and organic components of benzene(10.808), borny lacetate(11.289), phenol(14.183), ${\beta}$-terpinene(15.840), ${\alpha}$-terpinolene(17.616) etcs from Omija were ascertained with GC/MSD.

Trichome density of main stem is tightly linked to PepMoV resistance in chili pepper (Capsicum annuum L.)

Kim, Hyun Jung,Han, Jung-Heon,Kim, Seungill,Lee, Heung Ryul,Shin, Jun-Sung,Kim, Jeong-Ho,Cho, Juok,Kim, Young Ho,Lee, Hee Jae,Kim, Byung-Dong,Choi, Doil Springer-Verlag 2011 TAG. Theoretical and applied genetics. Theoretisch Vol.122 No.6

S-578 The serum galactomannan test in allogeneic stem cell transplantation recipients

( Ryul Kim ),( Youngil Koh ),( Dong-yeop Shin ),( Pyoeng Gyun Choe ),( Nam Joong Kim ),( Sung-soo Yoon ),( Myoung-don Oh ),( Wan Beom Park ),( Inho Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Objectives: We evaluated the outcomes of using the serum galactomannan (GM) assay for the screening of invasive pulmonary aspergillosis (IPA) in patients undergoing allogeneic hematopoietic stem cell transplantation (alloHSCT) for hematologic disorders while on primary antifungal prophylaxis (PAP). Methods: This study included patients with hematologic disorders who underwent alloHSCT from January 2013 to November 2015. The conditioning regimen of alloHSCT was tailored accord-ing to the underlying hematologic disease and the patient’s general condition. Patients received routine PAP with fluconazole prior to 2014 and micafungin after 2014; serum GM tests performed were retrospectively analyzed. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of serum GM tests for detection of proba-ble/proven IPA were evaluated. Results: A total of 136 alloHSCT recipients at Seoul National University Hospital were included in the study. Fluconazole was administered in 72 patients for PAP, while micafungin was admin-istered in the remaining 64 patients. The overall sensitivity, specificity, and NPV of serum GM assays were 95.8% (95% confidence interval [CI] 78.9-99.9%), 93.8% (95% CI 91.7-95.5%) and 99.8% (95% CI 99.1-100.0%), respectively. However, the PPV of GM tests was relatively low at 35.4% (95% CI 23.9-48.2%). The serial change of serum GM levels differed according to the antifungal agents used. With effective PAP using micafungin, serial serum GM levels show zero order kinetics during the neutropenic period. Conclusions: Although the serum GM assay is a sensitive and specific test for detecting IPA in alloHSCT recipients, its role for routine surveillance is limited in an era of effective PAP with micafungin.

<i>Mycobacterium</i> <i>parakoreense</i> sp. nov.,<i></i> a slowly growing non-chromogenic species related to <i>Mycobacterium koreense</i>, isolated from a human clinical specimen

Kim, Byoung-Jun,Hong, Seok-Hyun,Yu, Hee-Kyung,Park, Young-Gil,Jeong, Joseph,Lee, Seon Ho,Kim, Sung-Ryul,Kim, Kijeong,Kook, Yoon-Hoh,Kim, Bum-Joon International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.6

<P>A previously undescribed, slowly growing, non-chromogenic <I>Mycobacterium</I> strain (299<SUP>T</SUP>) was isolated from the sputum sample of a patient with a symptomatic pulmonary infection. Phenotypically, strain 299<SUP>T</SUP> was generally similar to <I>Mycobacterium koreense</I> DSM 45576<SUP>T</SUP> and <I>Mycobacterium triviale</I> ATCC 23292<SUP>T</SUP>. The 16S rRNA gene sequence of strain 299<SUP>T</SUP> was similar to that of <I>M. koreense</I> DSM 45576<SUP>T</SUP> (GenBank accession no. AY734996, 99.5 % similarity); however, it differed substantially from that of <I>M. triviale</I> ATCC 23292<SUP>T</SUP> (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 299<SUP>T</SUP> clustered together with <I>M. koreense</I> DSM 45576<SUP>T</SUP> and <I>M. triviale</I> ATCC 23292<SUP>T</SUP>, supported by high bootstrapping values (99 %). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, the <I>hsp65</I> and <I>rpoB</I> genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 299<SUP>T</SUP> represents a novel mycobacterial species, for which the name <I>Mycobacterium parakoreense</I> sp. nov. is proposed. The type strain is 299<SUP>T</SUP> ( = DSM 45575<SUP>T</SUP> = KCTC 19818<SUP>T</SUP>).</P>

Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale

Kim, Byoung-Jun,Jeong, Joseph,Lee, Seon Ho,Kim, Sung-Ryul,Yu, Hee-Kyung,Park, Young-Gil,Kim, Ki-Jeong,Kook, Yoon-Hoh,Kim, Bum-Joon Microbiology Society 2012 International journal of systematic and evolutiona Vol.62 No.6

<P>A novel slow-growing, non-chromogenic mycobacterium (strain 01-305<SUP>T</SUP>) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus <I>Mycobacterium</I>. Phenotypically, strain 01-305<SUP>T</SUP> was generally similar to <I>Mycobacterium triviale</I> ATCC 23292<SUP>T</SUP>, but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305<SUP>T</SUP> was similar to those of <I>M. triviale</I> ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and <I>M. triviale</I> ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of <I>M. triviale</I> ATCC 23292<SUP>T</SUP> (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305<SUP>T</SUP> in the slow-growing <I>Mycobacterium</I> group close to <I>M. triviale</I> ATCC 23290 and <I>M. triviale</I> ATCC 23291, but not <I>M. triviale</I> ATCC 23292<SUP>T</SUP>. Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the <I>hsp65</I> and <I>rpoB</I> genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305<SUP>T</SUP> represents a novel mycobacterial species, for which the name <I>Mycobacterium koreense</I> sp. nov. is proposed. The type strain is 01-305<SUP>T</SUP> ( = DSM 45576<SUP>T</SUP> = KCTC 19819<SUP>T</SUP>).</P>

Leaves of Persimmon (<i>Diospyros kaki</i> Thunb.) Ameliorate <i>N</i>-Methyl-<i>N</i>-nitrosourea (MNU)-Induced Retinal Degeneration in Mice

Kim, Kyung-A,Kang, Suk Woo,Ahn, Hong Ryul,Song, Youngwoo,Yang, Sung Jae,Jung, Sang Hoon American Chemical Society 2015 Journal of agricultural and food chemistry Vol.63 No.35

<P>The purpose of the study was to investigate the protective effects of the ethanol extract of <I>Diospyros kaki</I> (EEDK) persimmon leaves to study <I>N</I>-methyl-<I>N</I>-nitrosourea (MNU)-induced retinal degeneration in mice. EEDK was orally administered after MNU injection. Retinal layer thicknesses were significantly increased in the EEDK-treated group compared with the MNU-treated group. The outer nuclear layer was preserved in the retinas of EEDK-treated mice. Moreover, EEDK treatment reduced the MNU-dependent up-regulation of glial fibrillary acidic protein (GFAP) and nestin expression in Müller and astrocyte cells. EEDK treatment also inhibited MNU-dependent down-regulation of rhodopsin expression. Quercetin exposure significantly attenuated the negative effects of H<SUB>2</SUB>O<SUB>2</SUB> in R28 cells, suggesting that quercetin can act in an antioxidative capacity. Thus, EEDK may be considered as an agent for treating or preventing degenerative retinal diseases, such as retinitis pigmentosa and age-related macular degeneration.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2015/jafcau.2015.63.issue-35/acs.jafc.5b02578/production/images/medium/jf-2015-025787_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf5b02578'>ACS Electronic Supporting Info</A></P>