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Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.
Son, Jun Seong,Song, Jae-Hoon,Ko, Kwan Soo,Yeom, Joon Sup,Ki, Hyun Kyun,Kim, Shin-Woo,Chang, Hyun-Ha,Ryu, Seong Yeol,Kim, Yeon-Sook,Jung, Sook-In,Shin, Sang Yop,Oh, Hee Bok,Lee, Yeong Seon,Chung, Doo The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7
<P>Recent changes in healthcare systems have changed the epidemiologic paradigms in many infectious fields including bloodstream infection (BSI). We compared clinical characteristics of community-acquired (CA), hospital-acquired (HA), and healthcare-associated (HCA) BSI. We performed a prospective nationwide multicenter surveillance study from 9 university hospitals in Korea. Total 1,605 blood isolates were collected from 2006 to 2007, and 1,144 isolates were considered true pathogens. HA-BSI accounted for 48.8%, CA-BSI for 33.2%, and HCA-BSI for 18.0%. HA-BSI and HCA-BSI were more likely to have severe comorbidities. <I>Escherichia coli</I> was the most common isolate in CA-BSI (47.1%) and HCA-BSI (27.2%). In contrast, <I>Staphylococcus aureus</I> (15.2%), coagulase-negative <I>Staphylococcus</I> (15.1%) were the common isolates in HA-BSI. The rate of appropriate empiric antimicrobial therapy was the highest in CA-BSI (89.0%) followed by HCA-BSI (76.4%), and HA-BSI (75.0%). The 30-day mortality rate was the highest in HA-BSI (23.0%) followed by HCA-BSI (18.4%), and CA-BSI (10.2%). High Pitt score and inappropriate empirical antibiotic therapy were the independent risk factors for mortality by multivariate analysis. In conclusion, the present data suggest that clinical features, outcome, and microbiologic features of causative pathogens vary by origin of BSI. Especially, HCA-BSI shows unique clinical characteristics, which should be considered a distinct category for more appropriate antibiotic treatment.</P>
OH, Sang-Ik,JEON, Albert Byungyun,JUNG, Byeong-Yeal,BYUN, Jae-Won,GOTTSCHALK, Marcelo,KIM, Aeran,KIM, Jong Wan,KIM, Ha-Young JAPANESE SOCIETY OF VETERINARY SCIENCE 2017 The Journal of veterinary medical science Vol.79 No.4
<P><I>Streptococcus suis</I> is an important pig pathogen with potential for human transmission. The serotype distributions and phenotypic characteristics vary over time and among regions; however, little is known about the characteristics of <I>S. suis</I> isolates in Korea. In this study, 240 <I>S. suis</I> isolates collected from pigs in Korea in 2009–2010 were serotyped by coagglutination tests, subsequently screened for three virulence-associated genes (<I>mrp</I>, <I>epf</I> and <I>sly</I>) and tested for antimicrobial susceptibility. As for 80 isolates, the serotypes of which were relevant to human infections, clonal complexes (CCs) were further identified by PCR. Serotype 3 was the most prevalent (15.8%), followed by serotype 2 (15.0%), with geographical variation for each serotype. Overall, 55.4% of the isolates carried <I>mrp</I>, whereas only 3.8% carried <I>epf.</I> CC25 was the most prevalent (41.3%) and was related to serotypes 2 and 9. The isolates showed higher susceptibility to ampicillin (93.4%) and ceftiofur (90.8%) than to the other antimicrobial agents tested. The highest resistance rate was observed to tetracycline (98.0%), followed by erythromycin (88.8%). In addition, the resistance to certain antimicrobials was significantly associated, in part, with virulence-associated genes or serotypes. Therefore, continuous characterization of <I>S. suis</I> is essential for the benefit of veterinary and human medicine.</P>
Oh, Mi-Hwa,Hwang, Hee-Sung,Chung, Bo-Ram,Paik, Hyun-Dong,Han, Sang-Ha,Kang, Sun-Moon,Ham, Jun-Sang,Kim, Hyoun-Wook,Seol, Kuk-Hwan,Jang, Ae-Ra,Jung, Gyoo-Yeol Korean Society for Food Science of Animal Resource 2012 한국축산식품학회지 Vol.32 No.2
This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.
Oh-Seok Kwon,Sung-Mok Ha,이동하 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.12
In this paper, we propose the stiffness characteristics of compliant three segment leg that can have a self-stable region in slow and fast running. This proposition can contribute to reducing the control effort and enhancing the locomotion energy efficiency for the compliant three segment legged robot in slow and fast running. Previous research indicated that the running self-stable region of the spring-mass system is located in a relatively fast running region and that of the two segment leg is located in a relatively slow running region. In this paper, we analyze the stiffness characteristics of the spring-mass system and the two segment leg to explain the previous research results. From this analysis, we propose the stiffness characteristics of the compliant three segment leg with a self-stable region in slow and fast running. We further design the compliant three segment leg based on this proposition and check its structural stability. We examine the running self-stable region of this compliant three segment leg to determine whether it has a self-stable region in slow and fast running. We also examine the walking self-stable region of this compliant three segment leg.
( Ha-young Park ),( In-jae Oh ),( Bo Gun Kho ),( Tae-ok Kim ),( Hong-joon Shin ),( Cheol Kyu Park ),( Yong-soo Kwon ),( Yu-il Kim ),( Sung-chul Lim ),( Young-chul Kim ),( Yoo-duk Choi ) 대한결핵 및 호흡기학회 2019 Tuberculosis and Respiratory Diseases Vol.82 No.3
Background: Programmed death-ligand 1 (PD-L1), a transmembrane protein, binds to the programmed death-1 (PD- 1) receptor, and anti-PD-1 therapy enables immune responses against tumors. This study aimed to assess clinical characteristics of PD-L1 expression using immunohistochemistry among Korean patients with lung cancer. Methods: We retrospectively reviewed the data of patients with pathologically proven lung cancer from a single institution. PD-L1 expression determined by Tumor Proportion Score (TPS) was detected using 22C3 pharmDx (Agilent Technologies) and SP263 (Ventana Medical Systems) assays. Results: From July 2016 to July 2017, 267 patients were enrolled. The main histologic type was adenocarcinoma (69.3%). Most participants were smokers (67.4%) and had clinical stage IV disease (60.7%). In total, 116 (42%) and 58 (21%) patients had TPS ≥1% and ≥50%, respectively. The patients were significantly older in TPS ≥1% group than in TPS <1% group (64.83 ± 9.38 years vs. 61.73 ± 10.78 years, p=0.014), not in TPS ≥50% cutoff value (64.69 ± 9.39 vs. 62.36 ± 10.51, p= 0.178). Regarding histologic grade, higher proportions of poorly differentiated tumor were observed in the TPS ≥1% (40.8% vs. 25.8%, p=0.020) and TPS ≥50% groups (53.2% vs. 27.2%, p=0.004). Among 34 patients examined with 22C3 and SP263 assays, 27 had positive results in both assays, with a cutoff of TPS ≥1% (r=0.826; 95% confidence interval, 0.736-0.916). Conclusion: PD-L1 expression, defined as TPS ≥1%, was related to older age and poorly differentiated histology. There was a similar distribution of PD-L1 expression in both 22C3 and SP263 results.
Sawtooth Fingered Comb Drive Actuator for Greater Displacement
Ha Sang Wook,Oh Sang-Woo,Hahm Ju-Hee,Kim Kwon Hee,Pak James Jungho The Korean Institute of Electrical Engineers 2005 KIEE International Transactions on Electrophysics Vol.5C No.6
The electrostatic comb drive actuator is one of the main building blocks in the field of micro electro-mechanical systems (MEMS). Most of the comb actuators presented previously have fingers that are rectangular in shape which produce a stable, constant force output during actuation. The use of sawtooth fixed fingers in a comb drive, which were presumed to produce an increasing force output with displacement due to the increased number of regions where fringing force, the driving force of comb actuators, appear. The dimensions of the sawtooth were derived from finite element analysis (FEA) of simplified finger models with sawtooth type fingers of various dimension and were compared to the rectangular finger model that showed that the sawtooth type fingers have $7\~9$ times stronger driving force. Finally, comb drive actuators with sawtooth type and rectangular fingers were fabricated and although the gap was bigger, the comb actuator with sawtooth type fingers showed about 1.7 times greater electrostatic force than the one with rectangular fingers at equal driving voltages. In conclusion, using the proposed sawtooth type comb fingers in a comb drive makes it possible to increase its displacement or reduce the driving voltage.
Ha, Jang Ho,Jang, Hyun A.,Moon, Ki-Beom,Baek, Kwang Hyun,Choi, Gyung Ja,Choi, Doil,Cho, Hye Sun,Kwon, Suk Yun,Jeon, Jae-Heung,Oh, Sang-Keun,Kim, Hyun-Soon G. Fischer 2017 Journal of plant physiology Vol. No.
<P><B>Abstract</B></P> <P>We previously isolated <I>Nicotiana benthamiana</I> matrix metalloprotease 1 (<I>NMMP1</I>) from tobacco leaves. The <I>NMMP1</I> gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant’s defense against bacterial pathogens. Expression of <I>NMMP1</I> was strongly induced during interactions between tobacco and one of its pathogens, <I>Phytophthora infestans</I>. To elucidate the role of the <I>NMMP1</I> in defense of <I>N. benthamiana</I> against fungal pathogens, we performed gain-of-function and loss-of-function studies. <I>NMMP1</I>-overexpressing plants had stronger resistance responses against <I>P. infestans</I> infections than control plants, while silencing of <I>NMMP1</I> resulted in greater susceptibility of the plants to the pathogen. This greater susceptibility correlated with fewer <I>NMMP1</I> transcripts than the non-silenced control. We also examined cell death as a measure of disease. The amount of cell death induced by the necrosis-inducing <I>P. infestans</I> protein 1, PiNPP1, was dependent on <I>NMMP1</I> in <I>N. benthamiana</I>. Potato plants overexpressing <I>NMMP1</I> also had enhanced disease resistance against <I>P. infestans.</I> RT-PCR analysis of these transgenic potato plants revealed constitutive up-regulation of the potato defense gene <I>NbPR5. NMMP1</I>-overexpressing potato plants were taller and produced heavier tubers than control plants. We suggest a role for <I>NMMP1</I>in pathogen defense and development.</P>