Allelic variation of melanocortin-1 receptor locus in Saudi indigenous sheep exhibiting different color coats

Mahmoud, Ahmed H.,Mashaly, Ashraf M.,Rady, Ahmed M.,Al-Anazi, Khalid M.,Saleh, Amgad A. Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: This study was designed to characterize the DNA polymorphisms of the melanocortin-1 receptor (MC1R) gene in indigenous Saudi Arabian sheep breeds exhibiting different color coats, along with individuals of the Sawaknee breed, an exotic sheep imported from Sudan. Methods: The complete coding region of MC1R gene including parts of 3' and 5' untranslated regions was amplified and sequenced from three the indigenous Saudi sheep; Najdi (generally black, n = 41), Naeimi (generally white with brown faces, n = 36) and Herri (generally white, n = 18), in addition to 13 Sawaknee sheep. Results: Five single nucleotide polymorphisms (SNPs) were detected in the MC1R gene: two led to nonsynonymous mutations (c.218 T>A, p.73 Met>Lys and c.361 G>A, p.121 Asp>Asn) and three led to synonymous mutations (c.429 C>T, p.143 Tyr>Tyr; c.600 T>G, p.200 Leu>Leu, and c.735 C>T, p.245 Ile>Ile). Based on these five SNPs, eight haplotypes representing MC1R $E^d$ and $E^+$ alleles were identified among the studied sheep breeds. The most common haplotype (H3) of the dominant $E^d$ allele was associated with either black or brown coat color in Najdi and Sawaknee sheep, respectively. Two other haplotypes (H6 and H7) of $E^d$ allele, with only the nonsynonymous mutation A218T, were detected for the first time in Saudi indigenous sheep. Conclusion: In addition to investigating the MC1R allelic variation in Saudi indigenous sheep populations, the present study supports the assumption that the two independent nonsynonymous Met73Lys and Asp121Asn mutations in MC1R gene are associated with black or red coat colors in sheep breeds.

Investigation of photon, neutron and proton shielding features of H₃BO₃-ZnO-Na₂O-BaO glass system

Mhareb, M.H.A.,Alajerami, Y.S.M.,Dwaikat, Nidal,Al-Buriahi, M.S.,Alqahtani, Muna,Alshahri, Fatimh,Saleh, Noha,Alonizan, N.,Saleh, M.A.,Sayyed, M.I. Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.3

The current study aims to explore the shielding properties of multi-component borate-based glass series. Seven glass-samples with composition of (80-y)H₃BO₃-10ZnO-10Na₂O-yBaO where (y = 0, 5, 10, 15, 20, 25 and 30 mol.%) were synthesized by melt-quench method. Various shielding features for photons, neutrons, and protons were determined for all prepared samples. XCOM, Phy-X program, and SRIM code were performed to determine and explain several shielding properties such as equivalent atomic number, exposure build-up factor, specific gamma-ray constants, effective removal cross-section (Σ_R), neutron scattering and absorption, Mass Stopping Power (MSP) and projected range. The energy ranges for photons and protons were 0.015-15 MeV and 0.01-10 MeV, respectively. The mass attenuation coefficient (μ/ρ) was also determined experimentally by utilizing two radioactive sources (¹⁶⁶Ho and ¹³⁷Cs). Consistent results were obtained between experimental and XCOM values in determining μ/ρ of the new glasses. The addition of BaO to the glass matrix led to enhance the μ/ρ and specific gamma-ray constants of glasses. Whereas the remarkable reductions in Σ_R, MSP, and projected range values were reported with increasing BaO concentrations. The acquired results nominate the use of these glasses in different radiation shielding purposes.

Engineering Dependability to Embedded Systems Software via Tactics

Saleh H. Al-Daajeh,Rafa E. Al-Qutaish,Fuad Al-Qirem 보안공학연구지원센터 2011 International Journal of Software Engineering and Vol.5 No.4

Prediction of compressive strength of concrete using neural networks

Yousef A. Al-Salloum,Abid A. Shah,Saleh H. Alsayed,Tarek H. Almusallam,M.S. Al-Haddad,H. Abbas 사단법인 한국계산역학회 2012 Computers and Concrete, An International Journal Vol.10 No.2

This research deals with the prediction of compressive strength of normal and high strength concrete using neural networks. The compressive strength was modeled as a function of eight variables: quantities of cement, fine aggregate, coarse aggregate, micro-silica, water and super-plasticizer, maximum size of coarse aggregate, fineness modulus of fine aggregate. Two networks, one using raw variables and another using grouped dimensionless variables were constructed, trained and tested using available experimental data, covering a large range of concrete compressive strengths. The neural network models were compared with regression models. The neural networks based model gave high prediction accuracy and the results demonstrated that the use of neural networks in assessing compressive strength of concrete is both practical and beneficial. The performance of model using the grouped dimensionless variables is better than the prediction using raw variables.

Mechanical and Thermal Characteristics of Optimized Electrospun Nylon 6,6 Nanofibers by Using Taguchi Method

Saleh S. Abdelhady,Said H. Zoalfakar,M. A. Agwa,Ashraf A. Ali 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11

This study is an attempt to optimize the electrospinning process to produce minimum Nylon 6,6 nanofibers by using Taguchi statistical technique. Nylon 6,6 solutions were prepared in a mixture of formic acid (FA) and Dichloromethane (DCM). Design of experiment by using Taguchi statistical technique was applied to determine the most important processing parameters influence on average fiber diameter of Nylon 6,6 nanofiber produced by electrospinning process. The effects of solvent/nylon and FA/DCM ratio on average fiber diameter were investigated. Optimal electrospinning conditions were determined by using the signal-to-noise (S/N) ratio that was calculated from the electrospun Nylon 6,6 nanofibers diameters according to "the-smaller-the-better" approach. The optimum Nylon 6,6 concentration (NY%) and FA/DCM ratio were determined. The morphology of electrospun nanofibers is significantly altered by FA/DCM solvent ratio as well as Nylon 6,6 concentration. The smallest diameter and the narrowest diameter distribution of Nylon 6,6 nanofibers (166 ffi 33 nm) were obtained for 10 wt% Nylon 6,6 solution in 80 wt% FA and 20 wt% DCM. An increase of 118%, 280% and 26% in tensile strength, modulus of elasticity and elongation at break over as-cast was obtained, respectively. Glass transition temperature of Nylon 6,6 nanofibers were determined by using differential scanning calorimeter (DSC). Analysis of variance ANOVA shows that NY% is the most influential parameter.

Enhancing Knowledge, Beliefs, and Intention to Screen for Prostate Cancer via Different Health Educational Interventions: a Literature Review

Saleh, Ahmad M,Fooladi, Marjaneh M,Petro-Nustas, Wasileh,Dweik, Ghadeer,Abuadas, Mohammad H Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Prostate cancer is one of the most common cancers affecting men globally, constituting the sixth leading cause of cancer related death in males, and the eleventh leading cause of death from cancer in all age groups. In Jordan, prostate cancer is the third most common cancer in the male population, accounting for one third (6.2%) of cancer related deaths and in 2010 alone, 218 (9.4%) new cases were identified. Objective: To assess the effectiveness of different health education interventions aimed at enhancing knowledge, beliefs and intention to screen for prostate cancer. Materials and Methods: A literature search from January 2000 to April 2015 was conducted using the key words "prostate disease," "educational program," "knowledge," "prostate cancer," "demographic factors and prostate cancer," "knowledge and prostate cancer," "education for patients with prostate cancer," "factors that affect intention to screen," "knowledge, beliefs, and intention to screen for prostate cancer," "impact of prostate educational program on beliefs," and "impact of educational program on intention to screen." Results: Majority of studies reviewed indicated that men had low levels of knowledge regarding prostate cancer, and mild to moderate beliefs with good intention to screen for prostate cancer. Conclusions: Most studies indicated that men's knowledge levels about prostate cancer were poor and they had mild to moderate beliefs and intentions to screen for prostate cancer. Therefore, development of an assessment strategy based on the Health Belief Model seems essential. An effectively designed and implemented educational program can help identify the needs and priorities of the target population.

Characterization of Novel Trichoderma asperellum Isolates to Select Effective Biocontrol Agents Against Tomato Fusarium Wilt

Mahmoud H. El_Komy,Amgad A. Saleh,Anas Eranthodi,Younes Y. Molan 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.1

The use of novel isolates of Trichoderma with efficient antagonistic capacity against Fusarium oxysporum f. sp. lycopersici (FOL) is a promising alternative strategy to pesticides for tomato wilt management. We evaluated the antagonistic activity of 30 isolates of T. asperellum against 4 different isolates of FOL. The production of extracellular cell wall degrading enzymes of the antagonistic isolates was also measured. The random amplified polymorphic DNA (RAPD) method was applied to assess the genetic variability among the T. asperellum isolates. All of the T. asperellum isolates significantly reduced the mycelial growth of FOL isolates but the amount of growth reduction varied significantly as well. There was a correlation between the antagonistic capacity of T. asperellum isolates towards FOL and their lytic enzyme production. Isolates showing high levels of chitinase and β-1,3-glucanase activities strongly inhibited the growth of FOL isolates. RAPD analysis showed a high level of genetic variation among T. asperellum isolates. The UPGMA dendrogram revealed that T. asperellum isolates could not be grouped by their antagonistic behavior or lytic enzymes production. Six isolates of T. asperellum were highly antagonistic towards FOL and potentially could be used in commercial agriculture to control tomato wilt. Our results are consistent with the conclusion that understanding the genetic variation within Trichoderma isolates and their biochemical capabilities are required for the selection of effective indigenous fungal strains for the use as biocontrol agents.

Removal of Some Heavy Metals from Industrial Wastewater by Lemmna Minor

Mahmoud Saleh Al-Khafaji,Faris H. Al-Ani,Alaa F. Ibrahim 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.4

Treatment of industrial wastewater by constructed wetland system for the removal of heavy metals consisting of clarification andLemna minor (duckweed) system was studied under experimental conditions through design, construction and operating pilot plant. Thisplant was operated with a constant hydraulic retention time of 2 hr and 10 days for clarifier and duckweed tanks respectively. Thepotential of duckweed has been investigated to accumulate Cd, Cr, Ni and Pb present in a combined industrial wastewater. Theexperiments results show that the average removal efficiency of Cd, Cr, Ni and Pb were 44.93%, 32.26%, 74.48%, and 79.1 respectively. Duckweed plant is a poor accumulator for Cd. While it is a hyperaccumulator for Pb, Cr and Ni. Also, the removal percentages of theseheavy metals have significant positive linear correlation with the pH reduction percentage and increases in acidic solution (pH < 7). Therefore pH highly affects the removal efficiency of these metals from wastewater. Duckweed shows promise for the removal of heavymetals from industrial wastewater which makes duckweed a good species for phytoremediation activities. Duckweed based treatmentsystems are a cost-effective and reliable supply of acceptable quality water for river rehabilitation and reuse for other purposes.

A comparative study on effects of tadalafil, losartan, extracts of grape seed and ginko biloba on skin inflammation induced by cisplatin in rats

Basma H. Marghani,Walaa F. Awadin,Rasha M. Saleh,Ahmed I. Ateya 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.3

We investigated the effects of cisplatin (CDDP) on inflammation in general and skin inflammation in particular with application of different treatment models using tadalafil™ (Tad), losartan™ (Los), extracts of grape seed (G.S) and ginko biloba (G.B). Ninety healthy male albino rats were used in this experiment distributed randomly into six groups (n = 15). The duration of the experiment was four weeks. At the end of 1st, 2nd and 4th weeks of treatment, blood samples were subjected to RNA extraction and RT-PCR for interleukin (IL)-1B and IL-18 genes expression. The results showed a significant increase (P < 0.0001) in levels of mRNA genes expression of IL-1B and IL-18 in CDDP exposed rats when compared with control rats. Four weeks after treatment, expression fold changes significantly decreased in all treated groups with variable degrees where Los was the best to decrease the fold changes of IL-1B and IL-18 genes expression. Numbers of blood vessels, CD31 positive proliferating endothelial cells around preexisting skin vessels, CD34 positive blood vessels, perivascular inflammatory cells and total skin inflammatory cells were higher in untreated CDDP exposed rats when compared with control rats. Pathological evaluation revealed that Los followed by Tad then G.B were able to reduce the increased inflammatory markers in blood, skin inflammation and skin blood vessel density in CDDP exposed rats.

Research Articles : Optimal Culture Conditions for Mycelial Growth of Lignosus rhinocerus

( W. H. Lai ),( M. J. Siti Murni ),( D. Fauzi ),( O. Abas Mazni ),( N. M. Saleh ) 한국균학회 2011 Mycobiology Vol.39 No.2

Lignosus rhinocerus is a macrofungus that belongs to Polyporaceae and is native to tropical regions. This highly priced mushroom has been used as folk medicine to treat diseases by indigenous people. As a preliminary study to develop a culture method for edible mushrooms, the cultural characteristics of L. rhinocerus were investigated in a range of culture media under different environmental conditions. Mycelial growth of this mushroom was compared on culture media composed of various carbon and nitrogen sources in addition to C/N ratios. The optimal conditions for mycelial growth were 30˚C at pH 6 and 7. Rapid mycelial growth of L. rhinocerus was observed on glucose-peptone and yeast extract peptone dextrose media. Carbon and nitrogen sources promoting mycelial growth of L. rhinocerus were glucose and potassium nitrate, respectively. The optimum C/N ratio was approximately 10:1 using 2% glucose supplemented as a carbon source in the basal media.