Antifungal Activity of Silver Salts of Keggin-Type Heteropolyacids Against Sporothrix spp.

Luciana Da Silva Mathias,João Carlos De Aquino Almeida,Luis César Passoni,Cristiani Miranda David Gossani,Gabriel Bonan Taveira,Valdirene Moreira Gomes,Olney Vieira-Da-Motta 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Sporotrichosis is a chronic and subacute mycosis causing epidemiological outbreaks involving sick cats and humans in southeastern Brazil. The systemic disease prevails in cats and in humans, with the symptoms restricted to the skin of immunocompetent individuals. Under these conditions, the prolonged treatment of animals and cases of recurrence justify the discovery of new treatments for sporotrichosis. This work addresses the antifungal activity of silver salts of Keggin-type heteropolyacid salts (Ag-HPA salts) such as Ag3[PW12O40], Ag6[SiW10V2O40], Ag4[SiW12O40] and Ag3[PMo12O40] and interactions with the antifungal drugs itraconazole (ITC), terbinafine (TBF) and amphotericin B (AMB) on the yeast and mycelia forms of Sporothrix spp. Sporothrix spp. yeast cells were susceptible to Ag-HPA salts at minimum inhibitory concentration (MIC) values ranging from 8 to 128 μg/ml. Interactions between Ag3[PW12O40] and Ag3[PMo12O40] with itraconazole and amphotericin B resulted in higher antifungal activity with a reduction in growth and melanization. Treated cells showed changes in cell membrane integrity, vacuolization, cytoplasm disorder, and membrane detachment. Promising antifungal activity for treating sporotrichosis was observed for the Ag-HPA salts Ag3[PMo12O40] and Ag3[PW12O40], which have a low cost, high yield and activity at low concentrations. However, further evaluation of in vivo tests is still required.

Multi-Domain Model for Electric Traction Drives Using Bond Graphs

Silva, Luis I.,De La Barrera, Pablo M.,De Angelo, Cristian H.,Aguilera, Facundo,Garcia, Guillermo O. The Korean Institute of Power Electronics 2011 JOURNAL OF POWER ELECTRONICS Vol.11 No.4

In this work the Multi-Domain model of an electric vehicle is developed. The electric domain model consists on the traction drive and allows including faults associated with stator winding. The thermal model is based on a spatial discretization. It receives the power dissipated in the electric domain, it interacts with the environment and provides the temperature distribution in the induction motor. The mechanical model is a half vehicle model. Given that all models are obtained using the same approach (Bond Graph) their integration becomes straightforward. This complete model allows simulating the whole system dynamics and the analysis of electrical/mechanical/thermal interaction. First, experimental results are aimed to validate the proposed model. Then, simulation results illustrate the interaction between the different domains and highlight the capability of including faults.

Multi-Domain Model for Electric Traction Drives Using Bond Graphs

Luis I. Silva,Pablo M. de la Barrera,Cristian H. De Angelo,Facundo Aguilera,Guillermo O. Garcia 전력전자학회 2011 JOURNAL OF POWER ELECTRONICS Vol.11 No.4

In this work the Multi-Domain model of an electric vehicle is developed. The electric domain model consists on the traction drive and allows including faults associated with stator winding. The thermal model is based on a spatial discretization. It receives the power dissipated in the electric domain, it interacts with the environment and provides the temperature distribution in the induction motor. The mechanical model is a half vehicle model. Given that all models are obtained using the same approach (Bond Graph) their integration becomes straightforward. This complete model allows simulating the whole system dynamics and the analysis of electrical/mechanical/thermal interaction. First, experimental results are aimed to validate the proposed model. Then, simulation results illustrate the interaction between the different domains and highlight the capability of including faults.

Tibial-graft fixation methods on anterior cruciate ligament reconstructions: a literature review

( Vitor Luis Pereira ),( João Victor Medeiros ),( Gilvan Rodrigues Silva Nunes ),( Gabriel Taniguti De Oliveira ),( Alexandre Pedro Nicolini ) 대한슬관절학회 2021 대한슬관절학회지 Vol.33 No.-

Introduction: Anterior cruciate ligament (ACL) reconstruction is the most performed orthopedic surgical procedure. The result of ACL reconstructions depends on multiple technical variables, including tension to be applied to the graft for fixation, knee-flexion angle during fixation and the type of fixation to the bone. Objective: To carry out a survey of the literature with the best evidence on these themes. Methods: Literature review about methods of tibial-graft fixation in ACL reconstructions - tension applied at the time of fixation, type of graft fixation, and knee-flexion degree during tibial fixation. Results: Thirty studies on the selected topics were found. Most studies point to graft-tension levels close to 90 N to obtain the best results. Regarding the knee-flexion angle, multiple studies suggest that fixation at a 30° angle would bring superior biomechanical advantages. Regarding the type of implant for fixation, it is not possible to affirm the superiority of one method over another in clinical outcomes. Conclusions: There is no consensus on the best method for tibial fixation of the grafts in ACL reconstructions regarding tension, type of implant and knee-flexion angle. However, the analysis of the studies pointed to certain trends and allowed the drawing of specific conclusions.

Intron 4 VNTR (4a/b) Polymorphism of the Endothelial Nitric Oxide Synthase Gene Is Associated with Breast Cancer in Mexican Women

Ramírez-Patiño Ramiro,Figuera Luis Eduardo,Puebla-Pérez Ana María,Delgado-Saucedo Jorge Ivan,Legazpi-Macias María Magdalena,Mariaud-Schmidt Rocio Patricia,Ramos-Silva Adriana,Gutiérrez-Hurtado Itzae A 대한의학회 2013 Journal of Korean medical science Vol.28 No.11

The endothelial nitric oxide synthase (eNOS) gene plays an important role in several biological functions. Polymorphisms of the eNOS gene have been associated with cancer. It has been suggested that the VNTR 4 a/b polymorphism may affect the expression of eNOS and contributes to tumor promotion in the mammary gland. We examined the role of the eNOS4 a/b polymorphism by comparing the genotypes of 281 healthy Mexican women with the genotypes of 429 Mexican women with breast cancer (BC). The observed genotype frequencies for control and BC patients were 0.6% and 0.7% for a/a (polymorphic); 87% and 77% for a/a (wild type); and 12% and 22% for a/b respectively. We found that the odds ratio (OR) was 1.9, with a 95% confidence interval (95%CI) of 1.29-2.95, P=0.001 for genotypes a/a-a/b, b/c. The association was also evident when comparing the distribution of the a/a-a/b genotypes in patients with high levels of glutamate-oxaloacetate transaminase (SGOT) (OR, 1.93; 95% CI, 1.14-3.28; P=0.015); undergoing menopause with high levels of SGOT (OR, 2.0; 95% CI, 1.1-3.84); and with high levels of glutamic-pyruvic transaminase (SGPT) (OR, 3.5; 95% CI, 1.56-8.22). The genotypes a/a-a/b are associated with BC susceptibility in the analyzed samples from the Mexican population.

Intraoperative fluid therapy for video-assisted ovariohysterectomy in dogs

Marília Teresa de Oliveira,João Pedro Scussel Feranti,Gabriela Pesamosca Coradini,Rafael Oliveira Chaves,Luis Felipe Dutra Corrêa,Marcella Teixeira Linhares,Roberto Thiesen,Marco Augusto Machado Silva 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.3

Background: Intraoperative fluids are still poorly studied in veterinary medicine. In humans the dosage is associated with significant differences in postoperative outcomes. Objectives: The aim of this study is to verify the influence of three different fluid therapy rates in dogs undergoing video-assisted ovariohysterectomy. Methods: Twenty-four female dogs were distributed into three groups: G5, G10, and G20. Each group was given 5, 10, and 20 mL·kg−1·h−1 of Lactate Ringer, respectively. This study evaluated the following parameters: central venous pressure, arterial blood pressure, heart rate, respiratory rate, temperature, acid-base balance, and serum lactate levels. Additionally, this study evaluated the following urinary variables: urea, creatinine, protein to creatinine ratio, urine output, and urine specific gravity. The dogs were evaluated up to 26 h after the procedure. Results: All animals presented respiratory acidosis during the intraoperative period. The G5 group evidenced intraoperative oliguria (0.80 ± 0.38 mL·kg−1·h−1), differing from the G20 group (2.17 ± 0.52 mL·kg−1·h−1) (p = 0.001). Serum lactate was different between groups during extubation (p = 0.036), with higher values being recorded in the G5 group (2.19 ± 1.65 mmol/L). Animals from the G20 group presented more severe hypothermia at the end of the procedure (35.93 ± 0.61°C) (p = 0.032). Only the members of the G20 group presented mean potassium values below the reference for the species. Anion gap values were lower in the G20 group when compared to the G5 and G10 groups (p = 0.017). Conclusions: The use of lactated Ringer's solution at the rate of 10 mL·kg−1·h−1 seems to be beneficial in the elective laparoscopic procedures over the 5 or 20 mL·kg−1·h−1 rates of infusion.

Genetics components of rice root architecture and carbon isotopic fractionation parameters: a tracer for breeding in a water-saving irrigation management

Brito Giovani G.,Concenço Germani,Costa Vladimir E.,Fagundes Paulo Ricardo R.,da Silva-Filho João Luis,Parfitt José Maria B.,Magalhães Ariano,Silva Giovana T.,Jardim Thaís M.,Luccas Nathália Furtado,S 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1

Phenotyping is the major bottleneck in the efort to develop varieties of rice (Oryza sativa L.) suitable for growing under a water-saving irrigation management, such as alternate wetting and drying irrigation techniques (AWD). To analyze if the genotypic variability for carbon isotope discrimination (CID) in rice leaves could be used as a relatively high-throughput tracer to early select superior genotypes highlighting improved root architecture traits when submitted to AWD, a set of twenty varieties grown under semi-natural conditions were submitted to two water irrigation regimes, continuous fooding (CF) and AWD cycles. Coefcients of genetic variance (π2g) obtained for root architecture, micro-morphological and physiological traits were signifcant for all of them regardless of the adopted irrigation system, except to mean root diameter. The three signifcant principal components (PCs) with eigenvalue>1, explain most of the total variation across cycles and water regimes. For most of analyzed traits, the values of heritability coefcients were higher regardless of adopted irrigation management and trait category; for CID, the magnitudes of broad heritability at an individual level (greater than 0.80) were similar in the two irrigation techniques, evidencing that the success of the selection is independent of irrigation management. The higher CID values after three AWD cycles are associated with varieties with higher total root length and volume. To our knowledge, this is the frst study demonstrating the potential application of CID as a tracer to select root architecture traits in rice when water-saving irrigation management is of concern

GENERATION OF MAGNETIC FIELDS IN COSMOLOGICAL SHOCKS

MEDVEDEV MIKHAIL V.,SILVA LUIS O.,FIORE MASSIMILIANO,FONSECA RICARDO A.,MORI WARREN B. The Korean Astronomical Society 2004 Journal of The Korean Astronomical Society Vol.37 No.5

The origin of magnetic fields in the universe remains an outstanding problem in cosmology. We propose that these fields are produced by shocks during the large-scale structure formation. We discuss the mechanism of the field generation via the counter-streaming (Weibel) instability. We also show that these Weibel-generated fields are long-lived and weakly coupled to dissipation. Subsequent field amplification by the intra-cluster turbulence may also take place, thus maintaining the magnetic energy density close to equipartition.

Nitric oxide-inhibited chloride transport in cortical thick ascending limbs is reversed by 8-iso-prostaglandin-F2α

( Pablo D. Cabral ),( Guillermo B. Silva ),( Sandra T. Baigorria ),( Luis I. Juncos ),( Ebenezer I. O. Ajayi ),( Néstor H. García ) 대한신장학회 2022 Kidney Research and Clinical Practice Vol.41 No.6

Background: Sodium chloride (NaCl) reabsorption in the cortical thick ascending limb (cTAL) is regulated by opposing effects. Nitric oxide (NO) inhibits NaCl reabsorption while 8-iso-prostaglandin-F2α (8-iso-PGF2α) stimulates it. Their interaction has not been evaluated in the cTAL. Because 8-iso-PGF2α has considerable stability while NO is a free radical with a short half-life, we hypothesized that, in the cTAL, the inhibition of NaCl absorption will be reversed by 8-iso-PGF2α. Methods: Chloride absorption (J_Cl) was measured in isolated perfused cTALs and whether the activation of protein kinase A (PKA) is required for this interaction. Since cyclic adenosine monophosphate (cAMP) is a major messenger for the 8-iso-PGF2α signaling cascade, and NO inhibits J_Cl by decreasing cAMP bioavailability, we measured 8-iso-PGF2α-stimulated cAMP in the presence of sodium nitroprusside (SNP). Results: The NO donor, SNP (10^-6 M), decreased J_Cl by 41%, while luminal 8-iso-PGF2α (100 μM) increased J_Cl to 315 ± 46 pmol/ min/mm (p < 0.003), reversing the effects of the NO donor. SNP inhibited J_Cl, 8-iso-PGF2α failed to increase J_Cl in the presence of H89. Basal cAMP was 56 ± 13 fmol/min/mm, in the presence of SNP 57 ± 6 fmol/min/mm, and 8-iso-PGF2α increased it to 92 ± 2 fmol/min/mm (p < 0.04). Conclusion: We concluded that 1) NO-induced inhibition of J_Cl in the cTAL can be reversed by 8-iso-PGF2α, 2) 8-iso-PGF2α and NO interaction requires PKA to control J_Cl, and 3) in the presence of NO, 8-iso-PGF2α continues to stimulate J_Cl because NO cannot reverse 8-iso-PGF2α-stimulated cAMP level.

Genotype–environment interaction for milk production of Gyr cattle in Brazil and Colombia

Alejandra Maria Toro‑Ospina,Ricardo Antonio Faria,Dominguez-Castaño Pablo,Santana Mario Luiz,Gonzalez Luis Gabriel,Espasandin Ana Carolina,Silva Josineudson Augusto II Vasconcelos 한국유전학회 2023 Genes & Genomics Vol.45 No.2

Background Genotype by environment interactions (G × E) can play an important role in cattle populations and should be included in breeding programs in order to select the best animals for different environments. Objective The aim of this study was to investigate the G × E for milk production of Gyr cattle in Brazil and Colombia by applying a reaction norm model used genomics information, and to identify genomic regions associated with milk production in the two countries. Methods The Brazilian and Colombian database included 464 animals (273 cows and 33 sires from Brazil and 158 cows from Colombia) and 27,505 SNPs. A two-trait animal model was used for milk yield adjusted to 305 days in Brazil and Colombia as a function of country of origin, which included genomic information obtained with a single-step genomic reaction norm model. The GIBBS3F90 and POSTGSf90 programs were used. Results The results obtained indicate G × E based on the reranking of bulls between Brazil and Colombia, demonstrating environmental differences between the two countries. The findings highlight the importance of considering the environment when choosing breeding animals in order to ensure the adequate performance of their progeny. Within this context, the reranking of bulls and the different SNPs associated with milk production in the two countries suggest that G × E is an important effect that should be included in the genetic evaluation of Dairy Gyr cattle in Brazil and Colombia. Conclusion The Gyr breeding program can be optimized by choosing a selection environment that will allow maximum genetic progress in milk production in different environments within and between countries.