In vitro cytotoxicity and in vivo acute toxicity of selected polysaccharide hydrogels as pharmaceutical excipients

Kulkarni GT,Gowthanarajan K,Raghu C,Ashok G,Vijayan P Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.1

Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.

Comparative genome analysis to identify SNPs associated with high oleic acid and elevated protein content in soybean

Kulkarni, Krishnanand P.,Patil, Gunvant,Valliyodan, Babu,Vuong, Tri D.,Shannon, J. Grover,Nguyen, Henry T.,Lee, Jeong-Dong,Belzile, F. National Research Council of Canada, Conseil natio 2018 Genome Vol. No.

<P> The objective of this study was to determine the genetic relationship between the oleic acid and protein content. The genotypes having high oleic acid and elevated protein (HOEP) content were crossed with five elite lines having normal oleic acid and average protein (NOAP) content. The selected accessions were grown at six environments in three different locations and phenotyped for protein, oil, and fatty acid components. The mean protein content of parents, HOEP, and NOAP lines was 34.6%, 38%, and 34.9%, respectively. The oleic acid concentration of parents, HOEP, and NOAP lines was 21.7%, 80.5%, and 20.8%, respectively. The HOEP plants carried both FAD2-1A (S117N) and FAD2-1B (P137R) mutant alleles contributing to the high oleic acid phenotype. Comparative genome analysis using whole-genome resequencing data identified six genes having single nucleotide polymorphism (SNP) significantly associated with the traits analyzed. A single SNP in the putative gene Glyma.10G275800 was associated with the elevated protein content, and palmitic, oleic, and linoleic acids. The genes from the marker intervals of previously identified QTL did not carry SNPs associated with protein content and fatty acid composition in the lines used in this study, indicating that all the genes except Glyma.10G278000 may be the new genes associated with the respective traits. </P>

Hologram Quantitative Structure Activity Relationship Analysis of JNK Antagonists

Kulkarni, Seema A.,Madhavan, Thirumurthy The Basic Science Institute Chosun University 2015 조선자연과학논문집 Vol.8 No.2

c-Jun N-terminal kinase-3 (JNK3) is a member of the mitogen-activated protein kinase family (MAPK), and plays an important role in neurological disorders. Therefore, identification of selective JNK3 inhibitor may contribute towards neuroprotection therapies. In this work, we performed hologram quantitative structure-activity relationship (HQSAR) on a series of thiophene trisubstituted derivatives. The best predictions were obtained for HQSAR model with $q^2=0.628$ and $r^2=0.986$. Statistical parameters from the generated QSAR models indicated the data is well fitted and have high predictive ability. HQSAR result showed that atom, bond and chirality descriptors play an important role in JNK3 activity and also shows that electronegative groups is highly favourble to enhance the biological activity. Our results could be useful to design novel and selective JNK3 inhibitors.

THERMAL AND STRUCTURAL ANALYSIS OF CALANDRIA VESSEL OF A PHWR DURING A SEVERE ACCIDENT

Kulkarni, P.P.,Prasad, S.V.,Nayak, A.K.,Vijayan, P.K. Korean Nuclear Society 2013 Nuclear Engineering and Technology Vol.45 No.4

In a postulated severe core damage accident in a PHWR, multiple failures of core cooling systems may lead to the collapse of pressure tubes and calandria tubes, which may ultimately relocate inside the calandria vessel forming a terminal debris bed. The debris bed, which may reach high temperatures due to the decay heat, is cooled by the moderator in the calandria. With time, the moderator is evaporated and after some time, a hot dry debris bed is formed. The debris bed transfers heat to the calandria vault water which acts as the ultimate heat sink. However, the questions remain: how long would the vault water be an ultimate heat sink, and what would be the failure mode of the calandria vessel if the heat sink capability of the reactor vault water is lost? In the present study, a numerical analysis is performed to evaluate the thermal loads and the stresses in the calandria vessel following the above accident scenario. The heat transfer from the molten corium pool to the surrounding is assumed to be by a combination of radiation, conduction, and convection from the calandria vessel wall to the vault water. From the temperature distribution in the vessel wall, the transient thermal loads have been evaluated. The strain rate and the vessel failure have been evaluated for the above scenario.

Mapping QTLs for 100-seed weight in an interspecific soybean cross of Williams 82 (Glycine max) and PI 366121 (Glycine soja)

Kulkarni, Krishnanand P.,Asekova, Sovetgul,Lee, Dong-Ho,Bilyeu, Kristin,Song, Jong Tae,Lee, Jeong-Dong CSIRO Publishing 2017 Crop & pasture science Vol.68 No.2

<P> Seed weight can be an important component for soybean quality and yield. The objective of the present study was to identify quantitative trait loci (QTLs) for 100-seed weight by using 169 recombinant inbred lines (RILs) derived from the cross Williams 82 × PI 366121. The parental lines and RILs were grown for four consecutive years (2012-15) in the field. The seeds were harvested after maturity, dried and used to measure 100-seed weight. Analysis of variance indicated significant differences among the RILs for 100-seed weight. The environment had significant effect on seed-weight expression as indicated by the genotype × environment interaction. QTL analysis employing inclusive composite interval mapping of additive QTLs implemented in QTL IciMapping (Version 4.1) identified nine QTLs (LOD >3) on chromosomes 1, 2, 6, 8, 13, 14, 17 and 20. The individual QTLs explained phenotypic variation in the range 6.1-12.4%. The QTLs were detected in one or two environments, indicating major influence of the growing environment on seed-weight expression. Four QTLs identified in this study, qSW-02_1, qSW-06_1, qSW-13_1 and qSW-14_1, were found to be new QTLs. The findings of the study may be helpful to reveal the molecular genetic basis of the seed-weight trait in soybean. </P>

EXPERIMENTAL CHARACTERISATION OF VLSI INTERCONNECTS USED IN HIGH-SPEED MULTICHIP MODULES

Kulkarni,S. Y.,Murthy,K. V. V. 대한전자공학회 1995 ICVC : International Conference on VLSI and CAD Vol.4 No.1

The cross-talk voltage generated by on-chip and off chip circuitry is one of the major considerations in IC design and package for high-speed systems. These crosstalk effects increase as a result of shorter rise times, larger chip currents, greater die dimensions and smaller spacing between the circuit components on the chips and boards. As a consequence of this the system-performance is mainly governed by the performance of interconnection system-performance. Considerable amount of work has been done theoretically to characterize such interconnection performance. However, the experimental characterisation aspects of such high-speed interconnects has received very less attention. In this paper, an effort has been made to characterise such interconnects experimentally. The results obtained from such characterisation are used to validate the theoretical results.

“Spine Surgery Checklist”: A Step towards Perfection through Protocols

Kulkarni Arvind Gopalrao,Patel Jwalant Yogeshkumar,Asati Sanjeev,Mewara Navin 대한척추외과학회 2022 Asian Spine Journal Vol.16 No.1

Study Design: A retrospective study.Purpose: This study aimed to evaluate the effectiveness of a novel checklist that was designed specifically for the “spine-surgerysubspecialty” to reduce the incidence of some common preventable human errors and major perioperative complications in spine surgery.Overview of Literature: We propose a unique spine surgery-specific checklist that recognizes the risk factors, anticipates the possible human errors, and thus helps in preventing these errors. This checklist is associated with increased patient safety awareness, improved communication (keeps everyone updated regarding their responsibilities), reduction in the surgical claims, and reduction in the number of postoperative complications, including mortality.Methods: This retrospective pilot study was performed at single center on 858 spine surgery patients. The patients were divided into the following two groups: the study group (after implementation of the checklist [2016–2017]) and the control group (before the implementation of the checklist [2015–2016]). The incidence of common preventable human errors and major perioperative complications in spine surgeries were recorded and compared between the two groups.Results: The prevalence of wrong-level surgeries was 0%, and the overall prevalence of the preventable errors was 1.63% (7/428). The rate of adverse, near-miss, and no-harm events was 0.23% (1/428), 0.70% (3/428) and 0.70% (3/428), respectively. The preoperative, intraoperative, and postoperative errors were 0.70% (3/428), 0.23% (1/428), and 0.70 (3/428), respectively. The reoperation rate related to preventable errors reduced after the checklist was used. There were significant differences in the total preventable errors related to complications, such as infections, prolonged hospital stays, and unplanned hospital readmission/revision surgeries (p=0.001)Conclusions: The authors propose the first-of-its kind spine surgery-specific checklist that is comprehensive and involves perioperative parameters. The checklist is easy to use, safe, and effective for reducing the unforgiving errors and perioperative complications. However, its broader implementation would require validation in large, multi-center, randomized control studies.

Genetic Improvement of the Fatty Acid Biosynthesis System to Alter the ω-6/ω-3 Ratio in the Soybean Seed

Kulkarni, Krishnanand P.,Kim, Minsu,Song, Jong Tae,Bilyeu, Kristin D.,Lee, Jeong-Dong Springer New York LLC 2017 Journal of the American Oil Chemists' Society Vol. No.

Note: Real time optical sensing of alpha-radiation emitting radioactive aerosols based on solid state nuclear track detector.

Kulkarni, A,Ha, S,Joshirao, P,Manchanda, V,Bak, M S,Kim, T American Institute of Physics 2015 Review of scientific instruments Vol.86 No.6

<P>A sensitive radioactive aerosols sensor has been designed and developed. Its design guidance is based on the need for a low operational cost and reliable measurements to provide daily aerosol monitoring. The exposure of diethylene-glycol bis (allylcarbonate) to radiation causes modification of its physico-chemical properties like surface roughness and reflectance. In the present study, optical sensor based on the reflectance measurement has been developed with an aim to monitor real time presence of alpha radioactive aerosols emitted from thorium nitrate hydrate. The results shows that the fabricated sensor can detect 0.0157 kBq to 0.1572 kBq of radio activity by radioactive aerosols generated from (Th(NO3)4 ??? 5H2O) at 0.1 ml/min flow rate. The proposed instrument will be helpful to monitor radioactive aerosols in/around a nuclear facility, building construction sites, mines, and granite polishing factories.</P>

The effect of denture base surface pretreatments on bond strengths of two long term resilient liners

Kulkarni, Rahul Shyamrao,Parkhedkar, Rambhau The Korean Academy of Prosthodonitics 2011 The Journal of Advanced Prosthodontics Vol.3 No.1

PURPOSE. Purpose of this study was to evaluate effect of two surface treatments, sandblasting and monomer treatment, on tensile bond strength between two long term resilient liners and poly (methyl methacrylate) denture base resin. MATERIALS AND METHODS. Two resilient liners Super-Soft and Molloplast-B were selected. Sixty acrylic resin (Trevalon) specimens with cross sectional area of $10{\times}10$ mm were prepared and divided into two groups of 30 specimens each. Each group was surface treated (n = 10) by sandblasting (250 ${\mu}$ alumina particles), monomer treatment (for 180 sec) and control (no surface treatment). Resilient liners were processed between 2 poly(methyl methacrylate) surfaces, in the dimensions of $10{\times}10{\times}3$ mm. Tensile strength was determined with Instron Universal testing machine, at a crosshead speed of 5 mm/min; and the modes of failure (adhesive, cohesive or mixed) were recorded. The data were analyzed using one-way ANOVA, followed by Tukey HSD test (${\alpha}$= 0.05). RESULTS. Monomer pretreatment of acrylic resin produced significantly higher bond strengths when compared to sandblasting and control for both resilient liners (P < .001). Sandblasting significantly decreased the bond strength for both the liners when compared to monomer pretreatment and control (P < .001). Mean bond strength of Super-Soft lined specimens was significantly higher than Molloplast-B in various surface treatment groups (P < .05). CONCLUSION. Surface pretreatment of the acrylic resin with monomer prior to resilient liner application is an effective method to increase bond strength between the base and soft liner. Sandblasting, on the contrary, is not recommended as it weakens the bond between the two.