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- 저자 : Peng Zhengqiang (검색결과 4 건)
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Electrospun Fibrous Silk Fibroin/Poly(L-Lactic Acid) Scaffold for Cartilage Tissue Engineering
Weiwei Liu,Zhengqiang Li,Lu Zheng,Xiaoyan Zhang,Peng Liu,Ting Yang,Bing Han 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.5
For successful tissue engineering of articular cartilage, a scaffold with mechanical properties that match those of natural cartilage as closely as possible is needed. In the present study, we prepared a fibrous silk fibroin (SF)/poly(L-lactic acid) (PLLA) scaffold via electrospinning and investigated the morphological, mechanical, and degradation properties of the scaffolds fabricated using different electrospinning conditions, including collection distance, working voltage, and the SF:PLLA mass ratio. In addition, in vitro cell-scaffold interactions were evaluated in terms of chondrocyte adhesion to the scaffolds as well as the cytotoxicity and cytocompatibility of the scaffolds. The optimum electrospinning conditions for generating a fibrous SF/PLLA scaffold with the best surface morphology (ordered alignment and suitable diameter) and tensile strength (~1.5 MPa) were a collection distance of 20 cm, a working voltage of 15 kV, and a SF:PLLA mass ratio of S50P50. The degradation rate of the SF/PLLA scaffolds was found to be determined by the SF:PLLA mass ratio, and it could be increased by reducing the PLLA proportion. Furthermore, chondrocytes spread well on the fibrous SF/PLLA scaffolds and secreted extracellular matrix, indicating good adhesion to the scaffold. The cytotoxicity of SF/PLLA scaffold extract to chondrocytes over 24 and 48 h in culture was low, indicating that the SF/PLLA scaffolds are biocompatible. Chondrocytes grew well on the SF/PLLA scaffold after 1, 3, 5, and 7 days of direct contact, indicating the good cytocompatibility of the scaffold. These results demonstrate that the fibrous SF/PLLA scaffold represents a promising composite material for use in cartilage tissue engineering.
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Comestible and temperature effects on the biological traits of fall armyworms, Spodoptera frugiperda
Li Fen,Wang Likui,Lu Rongcai,Peng Zhengqiang,Malhat Farag,Lyu Baoqian,Wu Shaoying 한국곤충학회 2021 Entomological Research Vol.51 No.10
Spodoptera frugiperda is considered a serious pest for many crops throughout the world. Here, we develop an easy and quick-to-prepare solid medium with multiple benefits, including reductions in cost, post-rearing waste, storage space, and labor, for rearing S. frugiperda larvae. The body length and weight at each development stage showed no differences with those of the corn leaves treatment group (P > 0.05). The development time of F0 generation from the first to adults stage was 51.27 ± 4.62 days when the larvae were reared on artificial diet, which is slightly longer than the 50.69 ± 4.56 days for those reared on corn leaves diet (P > 0.05). The temperature changes have no effect on body length and weight when the fall armyworms are reared on artificial diet, however, the influence of temperature change on development was more obvious. The development time was significantly longer at 18°C and shorter at 35°C than that at 25°C (P < 0.05). Furthermore, in the age-specific two-sex life table method, the life table parameters (i.e., r, λ, R0, GRR) significantly decreased at 18°C and 35°C compared with those at 25°C. The age–stage survival rate of S. frugiperda was higher on artificial diet at 25°C than 18°C, 35°C, and on corn leaves at 25°C in all stages. The 35°C temperature change had more influence on the age–stage survival than the other temperatures. This study provided ideal indoor breeding conditions for fall armyworm and sufficient materials for further indoor and outdoor research.
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Tao Jin,Yuying Lin,Shichou Han,Guangchang Ma,Haibo Wen,Zhengqiang Peng 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
Five candidate species for biocontrol of Opisina arenosella Walker, a destructive pest of palm trees, were evaluated. To compare host performance, 5 Trichogramma species were allowed to parasitize O. arenosella eggs of different ages and egg densities. The parasitization capacity of two species, T. embryophagum and T. japonicum, were evaluated with respect to adult age. Treatments consisted of field with releases of T. embryophagum or T. japonicum set up in Danzhou, Hainan, and the pest densities was recorded periodically. The results showed that T. embryophagum, T. chilonis, and T. dendrolimi had a strong parasitic ability on 0-day-old O. arenosella eggs. In species comparisons, the parasitism rates of T. embryophagum on 1-day-old and 4-day-old eggs, and T. japonicum on 2-day-old, 3-day-old, and 5-day-old eggs of O. arenosella were significantly higher than those of other Trichogramma species. At densities of 40 and 35 eggs/tube (84.8 cm 3 ), the numbers of eggs parasitized by T. embryophagum and T. japonicum were the highest, at 29.9 and 24.6, respectively. During the first 24-h, the numbers of eggs parasitized by T. embryophagum and T. japonicum were 110.6 and 82.2, accounting for 84.7% and 59.5%, respectively, of the total parasitized eggs. After 2 months releases, the larvae and pupa density of O. arenosella under T. embryophagum and T. japonicun treatment were 0.95 and 0.94 individuals/leaflet, respectively, were significantly lower than untreated control (1.6 individuals/leaflet). These findings encouraged that T. embryophagum and T. japonicun are able to be used for biological control for O. arenosella.
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Mbuji Amosi Leonard,Gong Xueyan,Li Fen,Yang Lei,Shi Xueyan,Peng Zhengqiang,Zhang Ying,Wu Shaoying 한국곤충학회 2022 Entomological Research Vol.52 No.5
Cannibalism behaviour in the Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is among of liming factor for mass production of the larvae for laboratory experiment. To understand the change of cannibalism, larvae of 1st, 2nd, and 3rd were used to study cannibalism they were put on the 3rd, 4th, and 5th larvae growth stages in a density of 30, 40, 50, 60, and 70. In this study, the results showed that the functional response of the 3rd -5th instar larvae to the 2nd larvae and the 4th–5th instar larvae to the 3rd larvae and 5th and 6th instar larvae to the 4th larvae fitted well with the Holling II mode. The maximum predation of the 3rd-5th instar larvae on the 2nd instar larvae were 27.17, 74.63, and 232.6 respectively, the maximum predation of the 4th–5th instar larvae on the 3rd instar larvae were 76.92 and 11.82 respectively, and the maximum predation of the 5th–6th instar larvae on the 4th instar larvae were 15.83 and 10.40. The predation of the older larvae to the younger larvae increased with the increase of the density of the younger larvae. This research aims at selecting the minimum number of larvae that will be effective and sustainable for laboratory experiments and minimizing the development of cannibalism in S. frugiperda, reduces crowding for larvae management and promoting healthy larvae. This study provides a pavement of managing larvae ensuring massive indoor larvae rearing to reduce the challenge of inadequate larva for the experiment.
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