Interactions among Measles Virus Hemagglutinin, Fusion Protein and Cell Receptor Signaling Lymphocyte Activation Molecule (SLAM) Indicating a New Fusion-trimer Model

Peng Zhang,Ling Yun Li,Chun Lin Hu,Qin Xu,Xin Liu,Yi Peng Qi 한국생활환경학회 2005 BMB Reports Vol.38 No.4

MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Hu Liangcong,Xie Xudong,Xue Hang,Wang Tiantian,Panayi Adriana C.,Lin Ze,Xiong Yuan,Cao Faqi,Yan Chengcheng,Chen Lang,Cheng Peng,Zha Kangkang,Sun Yun,Liu Guodong,Yu Chenyan,Hu Yiqiang,Tao Ranyang,Zhou 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

MicroRNAs (miRNAs) broadly regulate normal biological functions of bone and the progression of fracture healing and osteoporosis. Recently, it has been reported that miR-1224-5p in fracture plasma is a potential therapy for osteogenesis. To investigate the roles of miR-1224-5p and the Rap1 signaling pathway in fracture healing and osteoporosis development and progression, we used BMMs, BMSCs, and skull osteoblast precursor cells for in vitro osteogenesis and osteoclastogenesis studies. Osteoblastogenesis and osteoclastogenesis were detected by ALP, ARS, and TRAP staining and bone slice resorption pit assays. The miR-1224-5p target gene was assessed by siRNA-mediated target gene knockdown and luciferase reporter assays. To explore the Rap1 pathway, we performed high-throughput sequencing, western blotting, RT-PCR, chromatin immunoprecipitation assays and immunohistochemical staining. In vivo, bone healing was judged by the cortical femoral defect, cranial bone defect and femoral fracture models. Progression of osteoporosis was evaluated by an ovariectomy model and an aged osteoporosis model. We discovered that the expression of miR-1224-5p was positively correlated with fracture healing progression. Moreover, in vitro, overexpression of miR-1224-5p slowed Rankl-induced osteoclast differentiation and promoted osteoblast differentiation via the Rap1-signaling pathway by targeting ADCY2. In addition, in vivo overexpression of miR-1224-5p significantly promoted fracture healing and ameliorated the progression of osteoporosis caused by estrogen deficiency or aging. Furthermore, knockdown of miRNA-1224-5p inhibited bone regeneration in mice and accelerated the progression of osteoporosis in elderly mice. Taken together, these results identify miR-1224-5p as a key bone osteogenic regulator, which may be a potential therapeutic target for osteoporosis and fracture nonunion.

A Robust Crack Filter Based on Local Gray Level Variation and Multiscale Analysis for Automatic Crack Detection in X-ray Images

Peng, Shao-Hu,Nam, Hyun-Do The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.4

Internal cracks in products are invisible and can lead to fatal crashes or damage. Since X-rays can penetrate materials and be attenuated according to the material’s thickness and density, they have rapidly become the accepted technology for non-destructive inspection of internal cracks. This paper presents a robust crack filter based on local gray level variation and multiscale analysis for automatic detection of cracks in X-ray images. The proposed filter takes advantage of the image gray level and its local variations to detect cracks in the X-ray image. To overcome the problems of image noise and the non-uniform intensity of the X-ray image, a new method of estimating the local gray level variation is proposed in this paper. In order to detect various sizes of crack, this paper proposes using different neighboring distances to construct an image pyramid for multiscale analysis. By use of local gray level variation and multiscale analysis, the proposed crack filter is able to detect cracks of various sizes in X-ray images while contending with the problems of noise and non-uniform intensity. Experimental results show that the proposed crack filter outperforms the Gaussian model based crack filter and the LBP model based method in terms of detection accuracy, false detection ratio and processing speed.

A visual shape descriptor using sectors and shape context of contour lines

Peng, Shao-Hu,Kim, Deok-Hwan,Lee, Seok-Lyong,Chung, Chin-Wan Elsevier 2010 Information sciences Vol.180 No.16

<P><B>Abstract</B></P><P>This paper describes a visual shape descriptor based on the sectors and shape context of contour lines to represent the image local features used for image matching. The proposed descriptor consists of two-component feature vectors. First, the local region is separated into sectors and their gradient magnitude and orientation values are extracted; a feature vector is then constructed from these values. Second, local shape features are obtained using the shape context of contour lines. Another feature vector is then constructed from these contour lines. The proposed approach calculates the local shape feature without needing to consider the edges. This can overcome the difficulty associated with textured images and images with ill-defined edges. The combination of two-component feature vectors makes the proposed descriptor more robust to image scale changes, illumination variations and noise. The proposed visual shape descriptor outperformed other descriptors in terms of the matching accuracy: 14.525% better than SIFT, 21% better than PCA-SIFT, 11.86% better than GLOH, and 25.66% better than the shape context.</P>

Case Reports : Paroxysmal Supraventricular Tachycardia in an Infant with Hand, Foot, and Mouth Disease

( Peng Hu ),( Shu Hou ),( Peng Fei Du ),( Jia Bin Li ),( Ying Ye ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.2

An 11-month-old male infant was admitted to our hospital with fever, fussiness, poor feeding, vomiting, and tachypnea for two days prior. Physical examination revealed sporadic papules and vesicles occurring on his hands, feet, face, and perianal mucosa. Enterovirus 71 was identified from both throat swab and vesicle fluid using virus isolation techniques. The patient`s heart rate fluctuated in a very narrow range from 180~210/beats/min regardless of his physiologic state. An electrocardiogram showed P-waves buried within or occurring just after regular, narrow, QRS complexes. The patient was diagnosed as having hand, foot, and mouth disease in combination with paroxysmal supraventricular tachycardia (PSVT). The child recovered well with symptomatic treatment, including intravenous administration of acyclovir, glucocorticoids, immunoglobulin, adenosine, and sotalol. PSVT was terminated within 36 hours of hospitalization. The skin lesions became crusted on the third day, and then proceeded to heal spontaneously. Here we report on this unusual case and review the associated literature. (Ann Dermatol 24(2) 200∼202, 2012)

The appropriate shape of the boundary transition section for a mountain-gorge terrain model in a wind tunnel test

Peng Hu,Yongle Li,Rui Kang,Haili Liao 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.20 No.1

Characterization of wind flows over a complex terrain, especially mountain-gorge terrain (referred to as the very complex terrain with rolling mountains and deep narrow gorges), is an important issue for design and operation of long-span bridges constructed in this area. In both wind tunnel testing and numerical simulation, a transition section is often used to connect the wind tunnel floor or computational domain bottom and the boundary top of the terrain model in order to generate a smooth flow transition over the edge of the terrain model. Although the transition section plays an important role in simulation of wind field over complex terrain, an appropriate shape needs investigation. In this study, two principles for selecting an appropriate shape of boundary transition section were proposed, and a theoretical curve serving for the mountain-gorge terrain model was derived based on potential flow theory around a circular cylinder. Then a two-dimensional (2-D) simulation was used to compare the flow transition performance between the proposed curved transition section and the traditional ramp transition section in a wind tunnel. Furthermore, the wind velocity field induced by the curved transition section with an equivalent slope of 30 was investigated in detail, and a parameter called the \'velocity stability factor\' was defined; an analytical model for predicting the velocity stability factor was also proposed. The results show that the proposed curved transition section has a better flow transition performance compared with the traditional ramp transition section. The proposed analytical model can also adequately predict the velocity stability factor of the wind field.

Numerical simulation of the neutral equilibrium atmospheric boundary layer using the SST k-ω turbulence model

Hu, Peng,Li, Yongle,Cai, C.S.,Liao, Haili,Xu, G.J. Techno-Press 2013 Wind and Structures, An International Journal (WAS Vol.17 No.1

Modeling an equilibrium atmospheric boundary layer (ABL) in an empty computational domain has routinely been performed with the k-${\varepsilon}$ turbulence model. However, the research objects of structural wind engineering are bluff bodies, and the SST k-${\omega}$ turbulence model is more widely used in the numerical simulation of flow around bluff bodies than the k-${\varepsilon}$ turbulence model. Therefore, to simulate an equilibrium ABL based on the SST k-${\omega}$ turbulence model, the inlet profiles of the mean wind speed U, turbulence kinetic energy k, and specific dissipation rate ${\omega}$ are proposed, and the source terms for the U, k and ${\omega}$ are derived by satisfying their corresponding transport equations. Based on the proposed inlet profiles, numerical comparative studies with and without considering the source terms are carried out in an empty computational domain, and an actual numerical simulation with a trapezoidal hill is further conducted. It shows that when the source terms are considered, the profiles of U, k and ${\omega}$ are all maintained well along the empty computational domain and the accuracy of the actual numerical simulation is greatly improved. The present study could provide a new methodology for modeling the equilibrium ABL problem and for further CFD simulations with practical value.

Solvothermal Synthesis of Bi₂O₂CO₃ Nanoplates for Efficient Photodegradation of RhB and Phenol under Simulated Solar Light Irradiation

Hu, Sheng-Peng,Xu, Cheng-Yan,Zhang, Bao-You,Pei, Yi,Zhen, Liang Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.10

Monodispersed $Bi_2O_2CO_3$ nanoplates with an average width of 320 nm and thicknesses of 50-90 nm were successfully synthesized by a simple solvothermal method in a mixture solution of polyethylene glycol and $H_2O$. The obtained nanoplates were characterized by means of XRD, FT-IR, SEM and TEM. The effect of surfactant sodium dodecyl benzene sulfonate on the morphology of $Bi_2O_2CO_3$ product was investigated. Under simulated solar light irradiation, $Bi_2O_2CO_3$ nanoplates exhibited superior photocatalytic activities towards the degradation of RhB as well as high chemical stability upon cycling photocatalytic test. The nanoplates also showed promising photodegradation ability for eliminating refractory pollutant of phenol. The excellent photocatalytic performance of $Bi_2O_2CO_3$ nanoplates as compared with P25-$TiO_2$ endows them as promising high efficiency photocatalysts.

An Improved Texture Feature Extraction Method for Recognizing Emphysema in CT Images

Peng, Shao-Hu,Nam, Hyun-Do The Korean Institute of IIIuminating and Electrica 2010 조명·전기설비학회논문지 Vol.24 No.11

In this study we propose a new texture feature extraction method based on an estimation of the brightness and structural uniformity of CT images representing the important characteristics for emphysema recognition. The Center-Symmetric Local Binary Pattern (CS-LBP) is first used to combine gray level in order to describe the brightness uniformity characteristics of the CT image. Then the gradient orientation difference is proposed to generate another CS-LBP code combining with gray level to represent the structural uniformity characteristics of the CT image. The usage of the gray level, CS-LBP and gradient orientation differences enables the proposed method to extract rich and distinctive information from the CT images in multiple directions. Experimental results showed that the performance of the proposed method is more stable with respect to sensitivity and specificity when compared with the SGLDM, GLRLM and GLDM. The proposed method outperformed these three conventional methods (SGLDM, GLRLM, and GLDM) 7.85[%], 22.87[%], and 16.67[%] respectively, according to the diagnosis of average accuracy, demonstrated by the Receiver Operating Characteristic (ROC) curves.

Application Research on Face Detection Technology based on OpenCV in Mobile Augmented Reality

Hu Peng 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.4

Augmented Reality (AR) is a new computer application and human-machine interaction derived from virtual reality. It has been widely applied in social each domain. With the improvement on the performance and the quality of built-in camera of smartphones, it is possible to have the augmented reality system to come true. And with people entertainment needs growing, mobile augmented reality based on face detection has become a research hotspot. This paper discusses the development and typical technology of face detection technology and mobile augmented reality. A development framework of the face detection technique based on OpenCV in mobile augmented reality application is proposed. And this essay takes the AR face book show as an example analyzes the key technology in the process of development and proves its feasibility and effectiveness.