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Ba Linh, Nguyen Thuy,Lee, Kap‐,Ho,Lee, Byong‐,Taek Wiley Subscription Services, Inc., A Wiley Company 2013 Journal of biomedical materials research. Part A Vol.101 No.8
<P><B>Abstract</B></P><P>New biodegradable mats was successfully obtained by functional polyvinyl alcohol (PVA)/Gelatin (GE) blend fiber mats containing different BCP amounts (20, 40, and 50 w/v%) of biphasic calcium phosphate (BCP) nanoparticles for bone regeneration. BCP nanoparticles were loaded and dispersed successfully in the PVA/GE fibrous matrix. The addition of BCP was found to have increased fiber diameter, tensile strength, osteoblast cell adhesion, proliferation, and protein expression. Compared to the others, the 50% BCP‐loaded electrospun PVA/GE fibers had the most favorable mechanical properties, cell attachment and growth, and protein expression. <I>In vivo</I> bone formation was examined using rat models, and increased bone formation was observed for the 50% BCP‐loaded electrospun PVA/GE blends within 2 and 4 weeks. This result suggests that the 50% BCP‐PVA/GE composite nanofiber mat has high potential for use in the field of bone regeneration and tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.</P>
Linh, Nguyen Thuy Ba,Abueva, Celine D G,Lee, Byong-Taek IOP Publishing 2017 Biomedical materials Vol.12 No.1
<P>An injectable, in situ forming hydrogel system capable of co-delivering human adipose-derived stem cells (hADSC) and platelet-derived growth factor (PDGF) was investigated as a new system for tissue engineering, envisaged to support vascularization. The system consists of tyramine-conjugated gelatin and hydroxyphenyl acetamide chitosan derivative. Both are soluble and stable at physiologic conditions, which is a key factor for retaining viable cells and active growth factor. In situ gelation involved enzymatic crosslinking using horseradish peroxidase as a catalyst and hydrogen peroxide as an oxidant. Gel formation occurred within 30-90 s by controlling the concentration of polymers. PDGF release showed adequate release kinetics within the intended period of time and hADSC showed good compatibility with the hydrogel formulation based on the in vitro assay and subcutaneous implantation into BALB/c-nu/nu nude female mice. Immunohistochemical analysis confirmed viability of delivered hADSC. Histological analysis showed no immune reaction and confirmed blood vessel formation. The results implicate the hydrogel as a promising delivery vehicle or carrier of both cell and growth factor, which support vascularization for tissue engineering applications.</P>
Linh, Nguyen Thuy Ba,Huyen, Nguyen Thi Dieu,Noh, Seok Kyun,Lyoo, Won Seok,Lee, Dong-Ho,Kim, Yongman Elsevier 2009 Journal of organometallic chemistry Vol.694 No.21
<P><B>Graphical abstract</B></P><P>Dinuclear half-titanocene bearing a xylene bridge, <I>ortho-</I> and <I>meta-</I>xylene, have been successfully synthesized and introduced for styrene polymerization. The activities of the catalysts with rigid <I>ortho</I>- and <I>meta</I>-xylene bridges were higher than those of catalysts with flexible pentamethylene bridges. Activity of the catalysts increased with higher temperature and [Al]:[Ti].</P><ce:figure></ce:figure> <P><B>Abstract</B></P><P>Half-titanocene is well-known as an excellent catalyst for the preparation of SPS (syndiotactic polystyrene) when activated with methylaluminoxane (MAO). Dinuclear half-sandwich complexes of titanium bearing a xylene bridge, <I>(Ti</I>Cl<SUB>2</SUB>L<I>)<SUB>2</SUB>{(μ-η<SUP>5</SUP>, η<SUP>5</SUP>-C<SUB>5</SUB>H<SUB>4</SUB>-ortho-(CH<SUB>2</SUB>–C<SUB>6</SUB>H<SUB>4</SUB>–CH<SUB>2</SUB>)C<SUB>5</SUB>H<SUB>4</SUB>}</I>, (<B>4</B> (L=Cl), <B>7</B> (L=O-2,6-<I>i</I>Pr<SUB>2</SUB>C<SUB>6</SUB>H<SUB>3</SUB>)) and <I>(Ti</I>Cl<SUB>2</SUB>L<I>)<SUB>2</SUB>{(μ-η<SUP>5</SUP>, η<SUP>5</SUP>-C<SUB>5</SUB>H<SUB>4</SUB>-meta-(CH<SUB>2</SUB>–C<SUB>6</SUB>H<SUB>4</SUB>–CH<SUB>2</SUB>)C<SUB>5</SUB>H<SUB>4</SUB>}</I> (<B>5</B> (L=Cl), <B>8</B>(L=O-2,6-<I>i</I>Pr<SUB>2</SUB>C<SUB>6</SUB>H<SUB>3</SUB>)), have been successfully synthesized and introduced for styrene polymerization. The catalysts were characterized by <SUP>1</SUP>H- and <SUP>13</SUP>C NMR, and elemental analysis. These catalysts were found to be effective in forming SPS in combination with MAO. The activities of the catalysts with rigid <I>ortho</I>- and <I>meta</I>-xylene bridges were higher than those of catalysts with flexible pentamethylene bridges. The catalytic activity of four dinuclear half-titanocenes increased in the order of <B>4</B><<B>5</B><<B>7</B><<B>8</B>. This result displays that the <I>meta</I>-xylene bridged catalyst is more active than the <I>ortho</I>-xylene bridged and that the aryloxo group at the titanium center is more effective at promoting catalyst activity compared to the chloride group at the titanium center. Temperature and ratio of [Al]:[Ti] had significant effects on catalytic activity. Polymerizations were conducted at three different temperatures (25, 40, and 70°C) with variation in the [Al]:[Ti] ratio from 2000 to 4000. It was observed that activity of the catalysts increased with increasing temperature, as well as higher [Al]:[Ti]. Different xylene linkage patterns (<I>ortho</I> and <I>meta</I>) were recognized to be a principal factor leading to the characteristics of the dinuclear catalyst due to its different spatial arrangement, causing dissimilar intramolecular interactions between the two active sites.</P>
Sukul, Mousumi,Nguyen, Thuy Ba Linh,Min, Young-Ki,Lee, Sun-Young,Lee, Byong-Taek Mary Ann Liebert 2015 Tissue engineering. Part A Vol.21 No.11
<P>Bone regeneration is a coordinated process mainly regulated by multiple growth factors. Vascular endothelial growth factor (VEGF) stimulates angiogenesis and bone morphogenetic proteins (BMPs) induce osteogenesis during bone healing process. The aim of this study was to investigate how these growth factors released locally and sustainably from nano-cellulose (NC) simultaneously effect bone formation. A biphasic calcium phosphate (BCP)-NC-BMP2-VEGF (BNBV) scaffold was fabricated for this purpose. The sponge BCP scaffold was prepared by replica method and then loaded with 0.5% NC containing BMP2-VEGF. Growth factors were released from NC in a sustainable manner from 1 to 30 days. BNBV scaffolds showed higher cell attachment and proliferation behavior than the other scaffolds loaded with single growth factors. Bare BCP scaffolds and BNBV scaffolds seeded with rat bone marrow mesenchymal stem cells were implanted ectopically and orthotopically in nude mice for 4 weeks. No typical bone formation was exhibited in BNBV scaffolds in ectopic sites. BMP2 and VEGF showed positive effects on new bone formation in BNBV scaffolds, with and without seeded stem cells, in the orthotopic defects. This study demonstrated that the BNBV scaffold could be beneficial for improved bone regeneration. Stem cell incorporation into this scaffold could further enhance the bone healing process.</P>
Son, So-Ra,Linh, Nguyen-Thuy Ba,Yang, Hun-Mo,Lee, Byong-Taek TaylorFrancis 2013 SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS Vol.14 No.1
<P>Scaffolds were fabricated by electrospinning using polycaprolactone (PCL) blended with poly(methyl methacrylate) (PMMA) in ratios of 10/0, 7/3, 5/5 and 3/7. The PCL/PMMA ratio affected the fiber diameter, contact angle, tensile strength and biological <I>in vitro</I> and <I>in vivo</I> properties of the scaffolds, and the 7/3 ratio resulted in a higher mechanical strength than 5/5 and 3/7. <I>In vitro</I> cytotoxicity and proliferation of MG-63 osteoblast cells on these blended scaffolds were examined by MTT assay, and it was found that PCL/PMMA blends are suitable for osteoblast cell proliferation. Confocal images and expression of proliferating cell nuclear antigen confirmed the good proliferation and expression of cells on the 7/3 PCL/PMMA fibrous scaffolds. <I>In vivo</I> bone formation was examined using rat models, and bone formation was observed on the 7/3 PCL/PMMA scaffold within 2 months. <I>In vitro</I> and <I>in vivo</I> results suggest that 7/3 PCL/PMMA scaffolds can be used for bone tissue regeneration.</P>