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Nagao, Tomokazu,Matsuzaki, Kazuki,Takahashi, Miho,Minamitani, Haruyuki Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
In this study, we investigated apoptotic cell death induced by photodynamic therapy using 5-aminolaevulinic acid (ALA-PDT) in human promyelocytic leukemia cells (HL-60). ALA-PDT induced apoptosis in HL-60 cells as confirmed by DNA agarose gel electrophoresis and nuclear staining with Hoechst 33342. The apoptotic cell death was inhibited by addition of broad-spectrum caspase inhibitor Z-Asp-CH$_2$-DCB, indicating that the apoptotic cell death was induced in a caspase-dependent manner. Actually, western blotting analysis revealed that caspase-3 was processed as early as 1.5 h after ALA-PDT. Cytoplasmic cytochrome c released from mitochondria was detected by western blotting. However, inhibitor of caspase-9, a cysteine protease located in the downstream of cytochrome c release, was not able to reduce the apoptotic cell death. Therefore, the mitochondrial apoptotic pathway was not involved in the ALA-PDT-induced apoptosis. On the other hand, it was found that ALA-PDT-induced apoptosis was clearly inhibited by pretreatment of caspase-8 inhibitor. These data suggest that caspase-8-mediated apoptotic pathway is important in ALA-PDT-induced cell death.
Nagao, K.,Osada, K.,Murai, A.,Okumura, J.,Kita, K. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.12
We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN. In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.
Nagao, K.,Takahara, Y.,Asai, H.,Hayashi, N.,Kitayama, T.,Yoshimura, Y.,Yokota, H.,Kita, Kazumi Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.8
The effect of dietary fatty acid supplementation on milk production, milk composition and plasma constituents in dairy cows was examined. Dietary fatty acids including mainly palmitic acid and stearic acid were given to cows from 2 weeks before and 8 weeks after parturition. Weekly gain of daily milk production was increased gradually during early lactation period and reached a plateau at 4 weeks after parturition. Weekly gain of daily milk production in lactating cows received dietary fatty acids was significantly higher than that of cows given a control diet alone. Although milk lactose concentration was slightly decreased by dietary fatty acid supplementation, milk fat and protein were not significantly influenced by dietary fatty acid supplementation. Dietary fatty acids did not affect plasma concentrations of triglyceride, non-esterified fatty acids, total cholesterol, high-density lipoprotein cholesterol and glucose during postpartum. It is suggested that dietary fatty acid supplementation has the potency to enhance energy balance and improve milk yield without any adverse effects on milk composition.
Nagao, K.,Haba, M. K.,Lee, J. I.,Kim, T.,Lee, M. J.,Park, C.,Jwa, Y. J.,Choi, B.-G. THE GEOCHEMICAL SOCIETY OF JAPAN 2016 Geochemical journal Vol.50 No.4
<P>The Jinju (H5) meteorite fell as four fragments in the Jinju area, Gyeonsangnam-do, South Korea, on March 9, 2014. The major element concentrations and noble gas isotopic compositions were determined for Jinju-1 and Jinju-2, stones of the first and second discoveries, respectively, by using X-ray fluorescence (XRF) analysis and noble gas mass spectrometry combined with total melting and stepwise heating noble gas extraction. The major element composition agrees well with that of equilibrated H chondrites. Very low contamination from terrestrial atmospheric noble gases was detected, which may be attributed to the rapid recovery of the stones within two days after the fall of the Jinju meteorite. A short cosmic ray exposure age of 2.4 My was obtained. The (U, Th)-He, and K-Ar ages are concordant at ca. 4.0 Gy, which suggests that no severe heating event occurred to cause a loss of He after the resetting at 4.0 Gy. Overabundances of trapped Ar and Kr, which were released at 800 degrees C, were found in addition to 'normal' Q gas of Ar, Kr and Xe at >= 1400 degrees C. The low-temperature component could be explained by Ar and Kr supplied by an adjacent impact region that were trapped when the minerals of the Jinju meteorite were formed during cooling. The heavy shock event that occurred at 4.0 Gy might have produced the observed numerous vugs and vapor growth crystals in this meteorite (Choi et al., 2015) and could have supplied the Ar and Kr to thermally weak minerals.</P>
Sharing Information for Event Analysis over the Wide Internet
Nagao, Masahiro,Koide, Kazuhide,Satoh, Akihiro,Keeni, Glenn Mansfield,Shiratori, Norio The Korea Institute of Information and Commucation 2010 Journal of communications and networks Vol.12 No.4
Cross-domain event information sharing is a topic of great interest in the area of event based network management. In this work we use data sets which represent actual attacks in the operational Internet. We analyze the data sets to understand the dynamics of the attacks and then go onto show the effectiveness of sharing incident related information to contain these attacks. We describe universal data acquisition system for event based management (UniDAS), a novel system for secure and automated cross-domain event information sharing. The system uses a generic, structured data format based on a standardized incident object description and exchange format (IODEF). IODEF is an XML-based extensible data format for security incident information exchange. We propose a simple and effective security model for IODEF and apply it to the secure and automated generic event information sharing system UniDAS. We present the system we have developed and evaluate its effectiveness.
Host-microbial Cross-talk in Inflammatory Bowel Disease
Hiroko Nagao-Kitamoto,Nobuhiko Kamada 대한면역학회 2017 Immune Network Vol.17 No.1
A vast community of commensal microorganisms, commonly referred to as the gut microbiota, colonizes the gastrointestinal tract (GI). The involvement of the gut microbiota in the maintenance of the gut ecosystem is two-fold: it educates host immune cells and protects the host from pathogens. However, when healthy microbial composition and function are disrupted (dysbiosis), the dysbiotic gut microbiota can trigger the initiation and development of various GI diseases, including inflammatory bowel disease (IBD). IBD, primarily includes ulcerative colitis (UC) and Crohn's disease (CD), is a major global public health problem affecting over 1 million patients in the United States alone. Accumulating evidence suggests that various environmental and genetic factors contribute to the pathogenesis of IBD. In particular, the gut microbiota is a key factor associated with the triggering and presentation of disease. Gut dysbiosis in patients with IBD is defined as a reduction of beneficial commensal bacteria and an enrichment of potentially harmful commensal bacteria (pathobionts). However, as of now it is largely unknown whether gut dysbiosis is a cause or a consequence of IBD. Recent technological advances have made it possible to address this question and investigate the functional impact of dysbiotic microbiota on IBD. In this review, we will discuss the recent advances in the field, focusing on host-microbial cross-talk in IBD.