심환좌심실기능부전을 동반한 만성신부전환자에서 완전폐색관상동맥의 경피적관상동맥확장술

이내희,조윤행 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

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경피적관상동맥확장술 도중 발생한 관상동막파열과 대동맥박리 급성페색증을 경피적방법으로 치료한 1예

이내희,조윤행 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

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직장암 수술 후 국소재발과 예후

김태윤,백무준,김성용,신응진,박내경,이문수,김창호,송옥평 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Locally recurrent rectal cancer is a difficult clinical problem. Local recurrence following treatment for rectal cancer is a major cause of morbidity and mortality. The reported range of local recurrence rates following surgical treatment for rectal cancer has varied from 4% to 50%. We retrospectively reviewed the charts of 142 rectal cancer patients from January 1991 to December 1999. Analysis of patients factors included age, gender, type of surgery, and adjuvant therapy. Tumors were assessed for level, stage, and grade. Local recurrence and distant metastases were also documented. Variables influencing local recurrence in this group were identified and disease-free survival is determined. Of the 142 patients(74 males, 68 females; mean age 62.6 years), 137 consecutive patients operated on for rectal cancer and followed up to December 2000 (range 23-118months) were analyzed. Local recurrence rate was 18.3%. Age and sex of patients, type of surgery, location of tumor in the rectum, size, morphology and grading of the tumor were all unrelated to the event under investigation. At Cox regression, the Dukes stage and the postoperative radiotherapy were the only independent prognostic factors for local recurrence, and Dukes stage (Hazard ratio=2.89, p<0.001) and local recurrence (Hazard ratio=3.31, p<0.01) were the only independent factors associated with improved survival.

Original Article : High effectiveness of peginterferon alfa-2a plus ribavirin therapy in Korean patients with chronic hepatitis C in clinical practice

( Nae Yun Heo ),( Young Suk Lim ),( Han Chu Lee ),( Yung Sang Lee ),( Kang Mo Kim ),( Kwan Soo Byun ),( Kwang Hyub Han ),( Kwan Sik Lee ),( Seung Woon Paik ),( Seung Kew Yoon ),( Dong Jin Suh ) 대한간학회 2013 Clinical and Molecular Hepatology(대한간학회지) Vol.19 No.1

Background/Aims: Identifying the impact of a patient`s ethnicity on treatment responses in clinical practice may assist in providing individualized treatment regimens for chronic hepatitis C (CHC). The effectiveness of standard peginterferon plus ribavirin therapy and the need for triple combination therapy with protease inhibitors in Koreans remain matters of debate. These issues were investigated in the present study. Methods: The clinical data of 272 treatment-na?ve Korean CHC patients who were treated in a community-based clinical trial (Clinical Trial group; n=51) and in clinical practice (Cohort group; n=221), were analyzed and compared. All were treated with standard protocols of peginterferon alfa-2a plus ribavirin therapy. Results: For patients with hepatitis C virus (HCV) genotype 1, the sustained virological response (SVR) rates in the Clinical Trial and Cohort groups were 81% (21/26) and 55% (58/106), respectively, by intention-to-treat (ITT) analysis ( P=0.02), and 100% (13/13) and 80% (32/40), respectively, in treatment-adherent patients ( P=0.18). For patients with HCV genotype 2, the SVR rates in these two groups were 96% (24/25) and 88% (101/115), respectively, by ITT analysis ( P=0.31). Adherence and treatment duration were independent predictors of SVR for genotypes 1 and 2, respectively ( P<0.01 for each). Korean patients with CHC achieved high SVR rates with peginterferon alfa-2a plus ribavirin in both the clinical trial and clinical practice settings. Conclusions: Measures to raise adherence to standard therapy in clinical practice may improve the SVR rates in these patients as effectively as adding protease inhibitors, thus obviating the need for the latter. (Clin Mol Hepatol 2013;19:60-69)

Polyelectrolyte Micropatterning Using Agarose Plane Stamp and a Substrate Having Microscale Features on Its Surface

Lee, Min-Jung,Lee, Nae-Yoon,Lee, Sang-Kil,Park, Sung-Su,Kim, Youn-Sang Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.10

We have introduced polyelectrolyte micro-patterning technique employing agarose plane stamp and a hard substrate having microscale features on its surface. With this method, chemically micropatterned surfaces with both positive and negative functionalities were successfully embedded in well-defined microstructures, and selective impartment of charge functionalities was confirmed by patterning bead bearing surface charge. Furthermore, this technique allows highly sensitive immobilization of protein onto targeted surface simply by endowing functionalities, which extends the potential of its use as a tool for high-throughput protein microarray and proteomics. Because plane agarose stamp is free of structures on its surface, there is no concern for pattern collapse, and the combination of agarose plane stamp with patterned substrate is more suited for selective protein patterning compared with adopting surface-patterned agarose stamp with flat substrate. Our technique using agarose plane stamp and a substrate having microscale features on its surface suggests a range of possible applications, including the micropatterning of biofunctionalized copolymer having polyelectrolyte block, immobilization of micro- and nanoparticle with biofunctionalities such as biotin and streptavidine, and establishing optoelectronic microstructures with micro-beads on various surfaces.

Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7

Lee, Nae-Yoon,Yang, Yoon-sun,Kim, Youn-Sang,Park, Sung-su Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.4

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 $\mu$g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

보조생식술(ART)치료를 받은 환자들에서 자궁근종이 임신에 미치는 영향

이우식,이정노,박찬,이숙환,박원식,남윤성,최동희,정미경,조용선,이학천,윤내영,곽인평,한세열,윤태기,차광열 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.12

목적 : 본 연구는 장막하근종, 근육층내의 근종, 또는 점막하 근종등의 출현이 보조생식술후 임신율과 착상율에 어떠한 영향을 미치는가를 보기 위해 시행되었다. 연구방법 : 1998년 1월 1일 부터 1998년 11월 30일 까지 본원에서 보조생식술(ART)을 시행받은 불임환자들 중 양극단의 연령을 제외한 26-45세 사이의 환자들을 대상으로 하였으며, 자궁근종이 진단된 환자들의 278예와 근종이 없는 환자들의 1180예를 비교하였다. 결과 : 이 연령범위내 전체적인 비교에서는 대조군(비근종군)과 근종군에서의 임신율이 각각 41.78%, 31.65%, 그리고 착상율은 각각 15.7%, 11.48%로 통계적으로 유의하게 근종군에서 낮았다. 그러나 평균연령이 대조군(33.1세)에서 보다 근종군(35.5세)에서 유의하게 높음을 알게 되었고, 이에 따라서 연령군들(26-30, 31-35, 36-4, 41-45)에 따른 근종군과 대조군을 각각 비교하였다. 그 결과 동일 연령군에서 근종군(장막하 근종SS, 근육층내 근종IM)과 대조군, 그리고 근종의 그룹들(SS, IM)간의 임신율과 착상율의 비교에서는 서로간에 유의한 차이를 볼 수 없었다. 그리고 점막하 근종그룹의 경우는 통계적 비교를 하기에는 그 예와 수가 너무 적어 결과를 알 수 없었다. 결론 : 일반적으로 근종군에서 상대적으로 낮은 임신율을 보고한 여러 논문에서와 달리, 본 연구에서는 동일한 각각의 연령군에서의 근종군과 대조군간의 임신율과 착상율의 차이가 없었다. 또한 근종의 위치에 따른 그룹별 임신율 및 착상율의 비교에서도 유의한 차이가 없음을 보였는데 이것은 근종이 근육층을 어느정도의 기준치 이상으로 침범했을 때에만 IM으로 분류한 국외의 일부 보고와 달리 근육층을 조금이라도 침범하면 IM군으로 분류한 본 연구에서의 차이 때문일 것으로 생각되며, 장막하 근종그룹과 근육층내 근종그룹의 분류 기준을 달리한다면 다른 결과를 보일 수도 있으리라 여겨진다. 또한, 근종이 임신에 미치는 영향이 다양함을 고려할 때, 근종의 크기 및 위치에 따른 SS군과 IM군 등의 체계적 분류 후 이에 따르는 전향적 연구가 진행된다면 임신에 대한 근종의 영향평가에 많은 도움이 될 것으로 본다. Objective : The purpose of this study was to examine whether the uterine leiomyomas [subserosal(SS), intramural(IM), submucosal(SM)] influence on the pregnancy rate(PR) and implantation rate(IR) of ART program. Methods : This study was done by the review of medical records of 1458 ART cycles from Jan. 1 of 1998 to Nov. 30 of 1998 at Infertility Medical Center, CHA General Hospital. We compared the PR and IR between 278 cycles of myoma group and 1180 cycles of control(non-myoma) group. Results : The mean age of patients with myomas was significantly higher than that of control group. Accordingly, we set the age range(24 - 46yrs) and divided it into four parts(26-30, 31-35, 36-40, 41-45) that did not show the significant difference in PR and IR. As a result, there were no significant differences of PR and IR among control, SS and IM within same age range. (We didn't know the result in SM myoma group because the number of SM myoma cases were very small.) Conclusion : To the contrary of our expectation, there was no significant difference in PR and IR between control and myoma groups. On the other hand, the result of no significant difference in PR and IR between myoma groups may will show different result if we apply different classification in categorizing SS and IM myomas.

Fabrication of a Hydrophilic Composite Mold and its Application for Various Soft Lithographic Techniques

Nae Yoon Lee,Min Jung Lee,Ju Ri Lim,Mihee Kim,Youn Sang Kim 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

A Simple Imprint Method for Multi-Tiered Polymer Nanopatterning on Large Flexible Substrates Employing a Flexible Mold and Hemispherical PDMS Elastomer

Lee, Nae Yoon,Kim, Youn Sang Hu@thig & Wepf 2007 Macromolecular Rapid Communications Vol.28 No.20

<P>This work reports a facile method to fabricate multi-tiered polymer nanopatterns on SU-8 by the combination of imprint- and photo-lithography. First, SU-8 is imprint patterned using a polymeric flexible mold with an anti-adhesion coating that is deposited on a transparent and flexible substrate, at room temperature under low pressure. Next, the resulting SU-8 nanopatterns are exposed to UV light through a chromium mask by a photolithographic process. Removal of the unexposed SU-8 leaves behind multi-tiered structures. The use of a hemispherical poly(dimethylsiloxane) pad facilitates the evacuation of trapped air during the imprinting process. Line/space patterns of 500 nm with the smallest line width of 200 nm were homogeneously imprint-patterned on SU-8 on a large flexible substrate, and three-tiered structures, ranging in thickness from 300 nm to 2 µm, were successfully formed.</P><P> <img src='wiley_img/10221336-2007-28-20-MARC200700362-gra001.gif' alt='wiley_img/10221336-2007-28-20-MARC200700362-gra001'> </P>

Amine-modified single-walled carbon nanotubes protect neurons from injury in a rat stroke model

Lee, Hyun Jung,Park, Jiae,Yoon, Ok Ja,Kim, Hyun Woo,Lee, Do Yeon,Kim, Do Hee,Lee, Won Bok,Lee, Nae-Eung,Bonventre, Joseph V.,Kim, Sung Su Springer Science and Business Media LLC 2011 Nature nanotechnology Vol.6 No.2

<P>Stroke results in the disruption of tissue architecture and is the third leading cause of death in the United States. Transplanting scaffolds containing stem cells into the injured areas of the brain has been proposed as a treatment strategy, and carbon nanotubes show promise in this regard, with positive outcomes when used as scaffolds in neural cells and brain tissues. Here, we show that pretreating rats with amine-modified single-walled carbon nanotubes can protect neurons and enhance the recovery of behavioural functions in rats with induced stroke. Treated rats showed less tissue damage than controls and took longer to fall from a rotating rod, suggesting better motor functions after injury. Low levels of apoptotic, angiogenic and inflammation markers indicated that amine-modified single-walled carbon nanotubes protected the brains of treated rats from ischaemic injury.</P>