http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Yesol,Shukla, Shruti,Ahmed, Maruf,Son, Seokmin,Kim, Myunghee,Oh, Sejong Korean Society for Food Science of Animal Resource 2012 한국축산식품학회지 Vol.32 No.6
The present study was aimed to produce a chicken polyclonal antibody against Cronobacter muytjensii and to develop an immunoassay for its detection. Purification of anti-C. muytjensii IgY from egg yolk was accomplished using various methods such as water dilution and salt precipitation. As a result, sodium dodecyl sulfate-polyacrylamide gel electrophoresis produced two bands around 30 and 66 kDa, corresponding to a light and a heavy chain, respectively. Indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was performed to determine the effectiveness of the chicken IgY against C. muytjensii. The optimum conditions for detecting C. muytjensii by indirect ELISA and checkerboard titration of the antigen revealed an optimum average absorbance at the concentration of 18 ${\mu}g/mL$, having ca. $10^8$ coated cells per well. The anti-C. muytjensii IgY antibody had high specificity for C. muytjensii and low cross-reactivity with other tested pathogens. In this assay, no cross-reactivity was observed with the other genera of pathogenic bacteria including Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Bacillus cereus, Enterobacter aerogenes, Salmonella Enteritidis and Listeria monocytogenes. In addition, detection of C. muytjensii in infant formula powder showed a low matrix effect on the detection curve of IC-ELISA for C. muytjensii, with similar detection limit of $10^5$ CFU/mL as shown in standard curve. These findings demonstrate that the developed method is able to detect C. muytjensii in infant formula powder. Due to the stable antibody supply without sacrificing animals, this IgY can have wide applications for the rapid and accurate detection of C. muytjensii in dairy foods samples.
A Stepwise Split Power-Driving Scheme With Automatic Slope Control for EMC-Enhanced LIN Transceiver
Kim, Seungmok,Kim, Daewung,Lee, Kwangmuk,Lee, Myunghee,Kim, Jae Joon IEEE 2018 IEEE Transactions on Vehicular Technology VT Vol.67 No.3
<P>This paper proposes a split power-driving scheme for electromagnetic interference robust design and its automatic stepwise digital slope control for suppression of spectral emission. This analog–digital hybrid method gives power saving effect up to 24.4%, and also production reliability can be improved by utilizing a failure detection and recovery scheme. For feasibility of the proposed electromagnetic compatibility (EMC) scheme, a Local Interconnect Network transceiver prototype is fabricated in a 0.18-μm BCD process, and experimentally verified to provide EMC-enhanced operation with low-cost and low-power overheads.</P>
Kim, Min-Jeong,Kwon, Chang Hyuk,Kim, Dong-In,Im, Hee Su,Park, Sungil,Kim, Ji Ho,Bae, Jin-Sik,Lee, Myunghee,Lee, Min Seob Korean Society of Medical Genetics and Genomics 2015 대한의학유전학회지 Vol.12 No.2
Purpose: We developed and validated a fetal trisomy detection method for use as a noninvasive prenatal test (NIPT) including a Clinical Laboratory Improvement Amendments (CLIA)-certified bioinformatics pipeline on a cloud-based computing system using both Illumina and Life Technology sequencing platforms for 221 Korean clinical samples. We determined the necessary proportions of the fetal fraction in the cell-free DNA (cfDNA) sample for NIPT of trisomies 13, 18, and 21 through a limit of quantification (LOQ) test. Materials and Methods: Next-generation sequencing libraries from 221 clinical samples and three positive controls were generated using Illumina and Life Technology chemistries. Sequencing results were uploaded to a cloud and mapped on the human reference genome (GRCh37/hg19) using bioinformatics tools. Based on Z-scores calculated by normalization of the mapped read counts, final aneuploidy reports were automatically generated for fetal aneuploidy determination. Results: We identified in total 29 aneuploid samples, and additional analytical methods performed to confirm the results showed that one of these was a false-positive. The LOQ test showed that the proportion of fetal fraction in the cfDNA sample would affect the interpretation of the aneuploidy results. Conclusion: Noninvasive chromosome examination (NICE), a CLIA-certified NIPT with a cloud-based bioinformatics platform, showed unambiguous success in fetus aneuploidy detection.
EFL Learners’ Perceptions of the Educational Value of University Extracurricular Writing Programs
Myunghee Kim 한국영어교과교육학회 2018 영어교과교육 Vol.17 No.1
The aim of this study was to explore learner perceptions of the educational value of extracurricular English writing programs for university students in South Korea. The program of this study was designed to help students improve their English writing by providing them with opportunities to meet with native English speaking teachers one-on-one in 30 minute sessions. After completing the 10 week program, an open-ended survey of 85 students and in-depth interviews with 12 of those students were conducted to investigate their perceptions of the educational value of the program. Results indicated that the main reason students participated in the program was to have regular class assignments and job applications edited, focusing on error corrections. Emphasizing one-on-one oral feedback, most participants expressed satisfaction with their experiences in the program. In addition, some students discovered their voices and became more active participants in the process of learning. This study highlights the importance of promoting EFL learners’ active roles in writing conferences, providing individualized oral feedback, and creating stress-free writing experiences.
Predictors and outcomes of unplanned readmission to a different hospital
Kim, Hongsoo,Hung, William W.,Paik, Myunghee Cho,Ross, Joseph S.,Zhao, Zhonglin,Kim, Gi-Soo,Boockvar, Kenneth International Society for Quality in Health Care 2015 International journal for quality in health care Vol.27 No.6
<P><B>Objectives</B></P><P>To examine patient, hospital and market factors and outcomes associated with readmission to a different hospital compared with the same hospital.</P><P><B>Design</B></P><P>A population-based, secondary analysis using multilevel causal modeling.</P><P><B>Setting</B></P><P>Acute care hospitals in California in the USA.</P><P><B>Participants</B></P><P>In total, 509 775 patients aged 50 or older who were discharged alive from acute care hospitals (index hospitalizations), and 59 566 who had a rehospitalization within 30 days following their index discharge.</P><P><B>Intervention</B></P><P>No intervention.</P><P><B>Main Outcome Measures(s)</B></P><P>Thirty-day unplanned readmissions to a different hospital compared with the same hospital and also the costs and health outcomes of the readmissions.</P><P><B>Results</B></P><P>Twenty-one percent of patients with a rehospitalization had a different-hospital readmission. Compared with the same-hospital readmission group, the different-hospital readmission group was more likely to be younger, male and have a lower income. The index hospitals of the different-hospital readmission group were more likely to be smaller, for-profit hospitals, which were also more likely to be located in counties with higher competition. The different-hospital readmission group had higher odds for in-hospital death (8.1 vs. 6.7%; <I>P</I> < 0.0001) and greater readmission hospital costs ($15 671.8 vs. $14 286.4; <I>P</I> < 0.001) than the same-hospital readmission group.</P><P><B>Conclusions</B></P><P>Patient, hospital and market characteristics predicted different-hospital readmissions compared with same-hospital readmissions. Mortality and cost outcomes were worse among patients with different-hospital readmissions. Strategies for better care coordination targeting people at risk for different-hospital readmissions are necessary.</P>
Detection of Foodborne Pathogens and Analysis of Aflatoxin Levels in Home-made Doenjang Samples
Myunghee Kim,Yo Sep Kim 한국식품영양과학회 2012 Preventive Nutrition and Food Science Vol.17 No.2
Doenjang is a traditional Korean fermented soybean product that provides a major source of protein. In this study, a total of 18 different home-made doenjang samples were examined for the presence of foodborne pathogens and the total aflatoxin levels. Using an enzyme-linked immunosorbent assay to assess microbial quality and potential public health risk, we showed that total coliform levels in the doenjang samples ranged from 0 to 4.43±2.32 ×10<SUP>6</SUP> CFU/g, and the maximum limit of Bacillus cereus was 4.67±2.0×10<SUP>5</SUP> CFU/g. However, other foodborne pathogens, such as Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella spp., were not detected among the tested samples. One of the samples (S3) showed a maximum limit of 42.2±9.1 μg/kg for aflatoxin levels, which was above the safety limit allowed by the Codex Alimentarius Commission (CAC) regulatory agency. Further research is necessary to determine whether and how doenjang safety can be improved via elimination/reduction of microbial contamination during fermentation and storage or using microbial starter cultures for its fermentation.