Infrared Photodetector based on (3-Aminopropyl)trimethoxysilane (APTMS)-treated Rhenium Diselenide (ReSe₂) films

최재웅,Muhammad Hasnain,강동호,Ali박진홍 한국진공학회 2018 한국진공학회 학술발표회초록집 Vol.2018 No.2

Effects of peripartal glucose precursor supplementation on lactation performance and metabolic health of primiparous and multiparous dairy cows

Akhtar Muhammad Uzair,Hifzulrahman,Pasha Talat Naseer,Avais Muhammad,Khan Nauman,Chishti Ghazanfar Ali,Ali Mubashar,Imran Muhammad,Tahir Muhammad Naeem,Naveed-ul-Haque Muhammad 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

Objective: Hyperketonemia remains a major metabolic issue of serious milk production and a major health concern in early lactation cows. Oral supplementation of glucose precursors (GP) can be used to prevent hyperketonemia in dairy cows. The objective of this study was to compare the beneficial effects of orally supplementing a mixture of GP on metabolic health indicators and milk production status of primiparous (PP) and multiparous (MP) dairy cows. Methods: Twenty-eight Holstein cows were blocked by expected date of parturition, previous lactation yield, and parity. The cows were randomly allocated to one of the four treatment groups (n = 7 cows/group) based on their parity and GP supplementation: i) PP cows fed basal diet only (PP-CON), ii) PP cows with oral supplementation of GP (PP-GP), iii) MP cows fed basal diet only (MP-CON), and iv) MP cows with oral supplementation of GP (MP-GP). Glucose precursor (glycoline liquid) was orally drenched (300 mL/d) in GP cows from 7 days prepartum through 7 days postpartum. Other than GP supplementation, all cows were fed similar pre- and postpartum basal diets. Results: In both pre- and postpartum periods, serum glucose concentration was increased, whereas β-hydroxybutyrate and free fatty acids were decreased in GP cows compared with the CON cows. Milk yield and milk components were statistically not different between GP and CON cows over the first 9 week of lactation. The yield of actual milk, energycorrected milk, 63-days cumulative milk, colostrum yield, and calf birth weight remained higher in MP cows compared with PP cows. Conclusion: Oral drenching of GP around calving can be recommended to successfully improve the metabolic health and reduce the negative effects of hyperketonemia not only in MP but also in PP dairy cows.

In-vitro assessment of food consumption, utilization indices and losses promises of leafworm, Spodoptera litura (Fab.), on okra crop

Ahmad Nawaz,Habib Ali,Muhammad Sufyan,Muhammad Dildar Gogi,Muhammad Jalal Arif,Abid Ali,Muhammad Qasim,Waqar Islam,Noman Ali,Imran Bodla,Madiha Zaynab,Khalid Ali Khan,Hamed A. Ghramh 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

The lepidopteran insect pests have significant importance in vegetable production. The present study was performed to investigate the baseline studies about the assessment of feeding and consumption potential, utilization indices and losses promises of leafworm, Spodoptera litura (Fab.) on Okra. The data regarding feeding potential, food utilization and consumption indices as well as losses of different larval instars were recorded and subjected to appropriate statistical analysis. The results showed that, in the beginning, the approximate digestibility of various instars was increase, e.g. third instar (51.36%–64.03%), fourth instar (63.42%–69.45%) and fifth instar (70.25%–76.10%). However, after a certain period, the digestibility was decreased and efficiency to convert the ingested food into biomass varied significantly. The consumption index values increased with an increase in time but the consumption and growth rate was declined of fourth instar larvae. The ingestion and digestion increased of third (10.01–13.06, 8.32–11.91 mg), fourth (11.27–17.28, 10.96–14.03 mg) and fifth (12.60–19.40, 11.93–15.28 mg) larval instars. The corrected weight of consumed leaves increased with a gain in body weight. However, in the third instar, a decline was observed on the last day of feeding. Maximum leaf area was consumed by fifth instar larvae (44.66 cm 2 ) followed by fourth (35.41 cm 2 ) and third (27.98 cm 2 ) instars. In conclusion, all the dependent parameters, including food utilization potential, consumption indices and losses were higher for fifth instar larvae than others. These results emphasized the re-establishment of fundamental (economic threshold level: ETL, economic injury level: EIL) integrated pest management concepts.

A Comparison of Artificial Neural Networks (ANN) and Local Linear Regression (LLR) Techniques for Predicting Monthly Reservoir Levels

Muhammad Ali Shamim,Muhammad Hassan,Sameel Ahmad,Muhammad Zeeshan 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.2

Storage dams play a very important role in irrigation especially during lean periods. For proper regulation one should make sure the availability of water according to needs and requirements. Normally regression techniques are used for the estimation of a reservoir level but this study was aimed to account for a non-linear change and variability of natural data by using Gamma Test, for input combination and data length selection, in conjunction with Artificial Neural Networking (ANN) and Local Linear Regression (LLR) based models for monthly reservoir level prediction. Results from both training and validation phase clearly indicate the usefulness of both ANN and LLR based prediction techniques for Water Management in general and reservoir level forecasting in particular, with LLR outperforming the ANN based model with relatively higher values of Nash-Sutcliffe model efficiency coefficnet (R2) and lower values of Root Mean Squared Error (RMSE) and Mean Biased Error (MBE). The study also demonstrates how Gamma test can be effectively used to determine the ideal input combination for data driven model development.

Genome-wide identification and expression analyses of WRKY transcription factor family members from chickpea (Cicer arietinum L.) reveal their role in abiotic stress-responses

Muhammad Waqas,Muhammad Tehseen Azhar,Iqrar Ahmad Rana,Farrukh Azeem,Muhammad Amjad Ali,Muhammad Amjad Nawaz,정규화,Rana Muhammad Atif 한국유전학회 2019 Genes & Genomics Vol.41 No.4

Background WRKY proteins play a vital role in the regulation of several imperative plant metabolic processes and pathways, especially under biotic and abiotic stresses. Although WRKY genes have been characterized in various major crop plants, their identification and characterization in pulse legumes is still in its infancy. Chickpea (Cicer arietinum L.) is the most important pulse legume grown in arid and semi-arid tropics. Objective In silico identification and characterization of WRKY transcription factor-encoding genes in chickpea genome. Methods For this purpose, a systematic genome-wide analysis was carried out to identify the non-redundant WRKY transcription factors in the chickpea genome. Results We have computationally identified 70 WRKY-encoding non-redundant genes which were randomly distributed on all the chickpea chromosomes except chromosome 8. The evolutionary phylogenetic analysis classified the WRKY proteins into three major groups (I, II and III) and seven sub-groups (IN, IC, IIa, IIb, IIc, IId and IIe). The gene structure analysis revealed the presence of 2–7 introns among the family members. Along with the presence of absolutely conserved signatory WRKY domain, 19 different domains were also found to be conserved in a group-specific manner. Insights of gene duplication analysis revealed the predominant role of segmental duplications for the expansion of WRKY genes in chickpea. Purifying selection seems to be operated during the evolution and expansion of paralogous WRKY genes. The transcriptome data-based in silico expression analysis revealed the differential expression of CarWRKY genes in root and shoot tissues under salt, drought, and cold stress conditions. Moreover, some of these genes showed identical expression pattern under these stresses, revealing the possibility of involvement of these genes in conserved abiotic stress–response pathways. Conclusion This genome-wide computational analysis will serve as a base to accelerate the functional characterization of WRKY TFs especially under biotic and abiotic stresses.

Metabolites of Marine Algae Collected from Karachi-coasts of Arabian Sea

Muhammad Shaiq Ali,Muhammad Jahangir,Muhammad Saleem,Muhammad Kashif Pervez,Shaista Hameed,Viqar Uddin Ahmad 한국생약학회 2000 Natural Product Sciences Vol.6 No.2

The ethanolic extracts of marine green, brown and red algae collected from Karachi coasts of Arabian Sea afforded a new enol-derivative of N-acylsphingosine named as coelarthenol (1) from Coelarthrum muelleri, two new glucose-derivatives named: botryenal (2) and botryenol (3) from Botryocladia leptopoda, α-tocopherol quinone (4) from Codium iyengarii, β-sitosterol and hexadecanoic acid from Stokeyia indica. The known constituents (4, β-sitosterol & hexadecanoic acid) have not been reported so far from their corresponding sources and the structures were determined through spectroscopic methods, whereas, the structures of new constituents (1-3) were elucidated with the aid of selective HMBC experiments. The phytotoxicity of 4 was also monitored.

Erratum to: Upstream signalling of mTORC1 and its hyperactivation in type 2 diabetes (T2D)

( Muhammad Ali ),( Shazia Anwer Bukhari ),( Muhammad Ali ),( Han-woong Lee ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.1

Mammalian target of rapamycin complex 1 (mTORC1) plays a major role in cell growth, proliferation, polarity, differentiation, development, and controls transitioning between anabolic and catabolic states of the cell. It collects almost all extracellular and intracellular signals from growth factors, nutrients, and maintains cellular homeostasis, and is involved in several pathological conditions including, neurodegeneration, Type 2 diabetes (T2D), obesity, and cancer. In this review, we summarize current knowledge of upstream signaling of mTORC1 to explain etiology of T2D and hypertriglyceridemia, in which state, the role of telomere attrition is explained. We discuss if chronic inhibition of mTORC1 can reverse adverse effects resulting from hyperactivation. In conclusion, we suggest the regulatory roles of telomerase (TERT) and hexokinase II (HKII) on mTORC1 as possible remedies to treat hyperactivation. The former inhibits mTORC1 under nutrientrich while the latter under starved condition. We provide an idea of TOS (TOR signaling) motifs that can be used for regulation of mTORC1.

Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals

Muhammad Arshad,Maryam Siddiqa,Saddaf Rashid,Imran Hashmi,Muhammad Ali Awan,Muhammad Arif Ali 한국산업안전보건공단 산업안전보건연구원 2016 Safety and health at work Vol.7 No.2

Background: Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. Methods: Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. Results: The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13-25 years) had significantly higher DNA tail length (7.04 ㎛) than the controls (0.94 ㎛). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01-0.31 mg/L). Conclusion: We found a strong correlation (R2 = 0.91) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers’ attitude to prevent harmful environmental and anthropogenic effects.

A comprehensive review on phytochemistry, bioactivity and medicinal value of bioactive compounds of pomegranate (Punica granatum)

Muhammad Modassar Ali Nawaz Ranjha,Bakhtawar Shafique,Lufeng Wang,Shafeeqa Irfan,Muhammad Naeem Safdar,Mian Anjum Murtaza,Muhammad Nadeem,Shahid Mahmood,Ghulam Mueen-ud-Din,Hafiz Rehan Nadeem 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.1

Pomegranate (Punica granatum) acts as a therapeutic fruit possessing different bioactive compounds. Different compartments of pomegranate (P. granatum) like seed, peel, juice and leaves are rich in potential bioactive compounds. Pomegranate contains sinapyl, coniferyl, ellagic acid, cinnamic acid, genistein, linoleic acid, anthocyanin, gallic acid, catechin, quercetin, rutin, kaempferol, cyanidin, punicalin, delphinidin, punicalagin, chlorogenic acid, coumaric acid, luteolin and pelletierine alkaloids, flavonoids, anthocyanin and caffeic acid. Pomegranate also exhibits significant anti-oxidative, anti-hypertensive, cardioprotective and anti-diabetic properties and anti-carcinogenic possessions including prevention of prostate cancer; reduction in colon inflammation, prevention of skin tumorigenesis, improvement in renal function and reduction in metastatic melanoma growth. This review summarizes the current available data on the phytochemistry, bioavailability and therapeutic importance of potential bioactive compounds of pomegranate.

Upstream signalling of mTORC1 and its hyperactivation in type 2 diabetes (T2D)

( Muhammad Ali ),( Shazia Anwer Bukhari ),( Muhammad Ali ),( Han-woong Lee ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.12

Mammalian target of rapamycin complex 1 (mTORC1) plays a major role in cell growth, proliferation, polarity, differentiation, development, and controls transitioning between anabolic and catabolic states of the cell. It collects almost all extracellular and intracellular signals from growth factors, nutrients, and maintains cellular homeostasis, and is involved in several pathological conditions including, neurodegeneration, Type 2 diabetes (T2D), obesity, and cancer. In this review, we summarize current knowledge of upstream signaling of mTORC1 to explain etiology of T2D and hypertriglyceridemia, in which state, the role of telomere attrition is explained. We discuss if chronic inhibition of mTORC1 can reverse adverse effects resulting from hyperactivation. In conclusion, we suggest the regulatory roles of telomerase (TERT) and hexokinase II (HKII) on mTORC1 as possible remedies to treat hyperactivation. The former inhibits mTORC1 under nutrient-rich while the latter under starved condition. We provide an idea of TOS (TOR signaling) motifs that can be used for regulation of mTORC1. [BMB Reports 2017; 50(12): 601-609]