http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Morishita, Hidetada,Tamiaki, Hitoshi Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
Regioselective 3$^1$-$^{18}$ O-labelling of chlorophyll derivatives possessing a 3-formyl group such as methyl (pyro) pheophorbide-d (3, 4) was carried out efficiently by a simple one-step procedure; by stirring a homogeneous solution of tetrahydrofuran and H$_2$$^{18}$ O containing a small amount of trifuluoroacetic acid.
Recombinant Protein Expression and Purification of the Human HMTase MMSET/NSD2
Morishita, Masayo,Mevius, Damiaan,Shen, Yunpeng,Di Luccio, Eric Institute of Agricultural Science and Technology 2013 慶北大農學誌 Vol.31 No.3
Chromatin remodelers that include histone methyl transferases (HMTases) are becoming a focal point in cancer drug development. The NSD family of three HMTases, NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L are bona fide oncogenes found aberrantly expressed in several cancers, suggesting their potential role for novel therapeutic strategies. Several histone modifiers including HMTase have clear roles in human carcinogenesis but the extent of their functions and regulations are not well understood, especially in pathological conditions. The extents of the NSDs biological roles in normal and pathological conditions remain unclear. In particular, the substrate specificity of the NSDs remains unsettled and discrepant data has been reported. NSD2/MMSET is a focal point for therapeutic interventions against multiple myeloma and especially for t(4;14) myeloma, which is associated with a significantly worse prognosis than other biological subgroups. Multiple myeloma is the second most common hematological malignancy in the United States, after non-Hodgkin lymphoma. Herein, as a first step before entering a pipeline for protein x-ray crystallography, we cloned, recombinantly expressed and purified the catalytic SET domain of NSD2. Next, we demonstrated the catalytic activities, in vitro, of the recombinantly expressed NSD2-SET on H3K36 and H4K20, its biological targets at the chromatin.
Morishita, Masayo,Cho, Minju,Ryu, Juhee,Mevius, Damiaan E.H.F.,Di Luccio, Eric 경북대학교 농업과학기술연구소 2010 慶北大農學誌 Vol.28 No.-
The epigenetic therapy of cancers is emerging as an effective and valuable approach to both chemotherapy and the chemoprevention of cancer. The utilization of epigenetic targets that include histone methyltransferase (HMTase), Histone deacetylatase, and DNA methyltransferase, are emerging as key therapeutic targets. SET containing proteins such as the HMTase Setd1b has been found significantly amplified in cancerous cells. In order to shed some light on the histone methyl transferase family, we cloned the Setd1b gene from Mus musculus and build a collection of vectors for recombinant protein expression in E.coli that will pave the way for further structural biology studies. We prospect the role of the Setd1b pathway in cancer therapy and detail its unique value for designing novel anti-cancer epigenetic-drugs.
Changing Images of Medieval Women Saints : From St. AEthelthryth to Margery Kempe of Lynn
Sono MORISHITA 한국서양중세사학회 2016 西洋中世史硏究 Vol.0 No.38
성인전과 특히 지역사회에서 추앙되는 숭배는 당시대의 사회적 상황을 반영한다. 본고에서는 먼저 마저리 켐프의 책에 기록된 성녀 에셀드레다와 마저리 켐프의 생애를 살펴보고자 한다. 중세 영국의 유명한 여성들인 성녀 에셀드레다와 마저리 켐프는 상당히 대조적인 인물이다. 이어서 성녀 에셀드레다의 생애와 마저리 켐프의 책을 비교함으로써 앵글로 색슨 시대에서부터 중세 말기까지 성녀들의 이미지가 어떻게 변화하고 있는지를 설명하고자 한다. 성녀들의 이미지는 변하고 있다. 성녀 에셀드레다와 마저리 켐프의 이미지는 사뭇 달라 보이지만 유사한 문제점을 드러내고 있다. 그녀들의 이미지는 당시대 여성들의 사회적·종교적 상황을 이해할 수 있는 실마리를 제공하고 있다. Hagiographies reflect the social circumstances of the time, and especially the cults being practiced in the local community. In this paper, I will first examine the lives of St AEthelthryth and Margery Kempe, as documented in the Book of Margery Kempe. St. AEthelthryth and Margery Kempe are both famous women from medieval England, and they are quite contrasting women. I will then compare the life of St. AEthelthryth and the Book of Margery Kempe, to illustrate the changing image of female saints from the Anglo-Saxon Age to the late Medieval Age. Image of women saints changes. Images of St. AEthelthryth and Margery look different, but both images reveal similar problems, and both images give us clues to help us understand women’s social and religious circumstances in those days.