CULTURAL TOURISM

Morena Pauliš,ić 세계문화관광학회 2009 Conference Proceedings Vol.10 No.0

This paper is based on the assumption that culture and heritage if closely connected create benefits to destination. The benefits are especially present in point to give spirit, attraction, self -expression as well as give original, added value to the destination. The presented study findings showed us that cultural tourism in Istria has been generating a significant amount of travel, tourists' experience of authentic, unique, distinctive heritage but all happenings depend on some creative and persistence individuals, tourists orientated organizations and local government. The research confirmed that most popular and interesting cultural event always include symbiosis of culture and heritage.

Pharmacological and Neuromodulatory Treatments for Panic Disorder: Clinical Trials from 2010 to 2018

Morena M,Zugliani,Mariana C,Cabo,Antonio E,Nardi,Giampaolo Perna,Rafael C,Freire 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.1

Objective : Treatment for panic disorder (PD) have evolved, although there is still a strong unmet need for more effective and tolerable options. The present study summarizes and discusses recent evidence regarding the pharmacological and neuromodulatory treatment of PD. Methods : MEDLINE, Cochrane Library, PsycINFO and Thomson Reuters’s Web of Science were searched for clinical trials published between 2010 and 2018. We included all prospective experimental studies including randomized controlled trials (RCT) and other clinical trials with more than 10 patients. Results : Only 11 articles met the inclusion criteria, including 4 RCT, 3 open clinical trials and 5 comparative clinical trials. RCT demonstrated efficacy of transcranial magnetic stimulation (TMS) in only one of two trials. Neither pindolol nor d-fenfluramine were effective in blocking flumazenil-induced panic attacks. Augmentation with quetiapine was not superior to placebo. Open trials indicated that escitalopram, vortioxetine and TMS may be effective. Comparative trials did not demonstrate superiority from any drug, but confirmed tranylcypromine, paroxetine, clonazepam and alprazolam as effective options. Conclusion : The current study confirmed the efficacy of tranylcypromine, paroxetine, clonazepam, alprazolam and escitalopram. Vortioxetine and TMS, with duration of 4 or more weeks, also seems to be effective. Quetiapine, pindolol and d-fenfluramine were not considered effective compounds.

Effect of Dipeptidyl Peptidase-4 Inhibitors on the Risk of Bone Fractures in a Korean Population

Morena Ustulin,박소영,최항석,전숙,우정택,이상열 대한의학회 2019 Journal of Korean medical science Vol.34 No.35

Background: There have been equivocal results in studies of the effects of dipeptidyl peptidase-4 inhibitors (DPP-4i) on fractures. In this study, we analyzed the effect of DPP-4i on bone fracture risk in a Korean population. Methods: We extracted subjects (n = 11,164) aged 50 years or older from the National Health Insurance Service–National Sample Cohort 2.0 from 2009 to 2014. Our control group included subjects without diabetes (n = 5,582), and our treatment groups with diabetes included DPP-4i users (n = 1,410) and DPP-4i non-users (n = 4,172). The primary endpoint was the incidence of a composite outcome consisting of osteoporosis diagnosis, osteoporotic fractures, vertebral fractures, non-vertebral fractures, and femoral fractures. The secondary endpoint was the incidence of each individual component of the composite outcome. Survival analysis was performed with adjustment for age, gender, diabetes complications severity index, Charlson comorbidity index, hypertension medication, and dyslipidemia treatment. Results: The incidence of the composite outcome per 1,000 person-years was 0.089 in DPP-4i users, 0.099 in DPP-4i non-users, and 0.095 in controls. There was no significant difference in fracture risk between DPP-4i users and DPP-4i non-users or controls after the adjustments (P > 0.05). The incidences of osteoporosis diagnosis, osteoporotic fractures, vertebral fractures, non-vertebral fractures, and femoral fractures were not significantly different between DPP-4i users and non-users. The results of subgroup analyses by gender and age were consistent. Conclusion: DPP-4i had no significant effect on the risk of fractures in a Korean population.

Air Pollution Has a Significant Negative Impact on Intentional Efforts to Lose Weight: A Global Scale Analysis

Morena Ustulin,박소영,진상욱,전숙,우정택,이상열 대한당뇨병학회 2018 Diabetes and Metabolism Journal Vol.42 No.4

Background: Air pollution causes many diseases and deaths. It is important to see how air pollution affects obesity, which is common worldwide. Therefore, we analyzed data from a smartphone application for intentional weight loss, and then we validated them. Methods: Our analysis was structured in two parts. We analyzed data from a cohort registered to a smartphone application in 10 large cities of the world and matched it with the annual pollution values. We validated these results using daily pollution data in United States and matching them with user information. Body mass index (BMI) variation between final and initial login time was considered as outcome in the first part, and daily BMI in the validation. We analyzed: daily calories intake, daily weight, daily physical activity, geographical coordinates, seasons, age, gender. Weather Underground application programming interface provided daily climatic values. Annual and daily values of particulate matter PM10 and PM2.5 were extracted. In the first part of the analysis, we used 2,608 users and then 995 users located in United States. Results: Air pollution was highest in Seoul and lowest in Detroit. Users decreased BMI by 2.14 kg/m2 in average (95% confidence interval, –2.26 to –2.04). From a multilevel model, PM10 (β=0.04, P=0.002) and PM2.5 (β=0.08, P<0.001) had a significant negative effect on weight loss when collected per year. The results were confirmed with the validation (βAQI*time=1.5×10-5; P<0.001) by mixed effects model. Conclusion: This is the first study that shows how air pollution affects intentional weight loss applied on wider area of the world.

Characterization of Ligands-Conjugated AuNPs by Using ToF-SIMS Imaging Technique

Hyun Kyong Shon,Morena SAM Xaba,Mary Gulumian,Nam Woong Song,Tae Geol Lee 한국진공학회 2013 한국진공학회 학술발표회초록집 Vol.2013 No.2

Development of a multiphysics numerical solver for modeling the behavior of claybased engineered barriers

Vicente Navarro,Laura Asensio,Heidar Gharbieh,Gema De la Morena,Veli-Matti Pulkkanen 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.4

This work describes the development of a numerical module with a multiphysics structure to simulatethe thermo-hydro-chemo-mechanical behavior of compacted bentonites. First, the conceptual model,based on the state-of-the-art formulation for clay-based engineered barriers in deep geological repositories,is described. Second, the advantages of multiphysics-based modules are highlighted. Then, theguidelines to develop a code using such tools are outlined, presenting an example of implementation. Finally, the simulation of three tests that illustrate the behavior of compacted bentonites assesses thescope of the developed code. The satisfactory results obtained, and the relative simplicity of implementation,show the opportunity of the modeling strategy proposed

Reference Values for a Panel of Cytokinergic and Regulatory Lymphocyte Subpopulations

Vincenzo Sorrenti,Bruno Marenda,Stefano Fortinguerra,Claudia Cecchetto,Roberta Quartesan,Giulia Zorzi,Morena Zusso,Pietro Giusti,Alessandro Buriani 대한면역학회 2016 Immune Network Vol.16 No.6

Lymphocyte subpopulations producing cytokines and exerting regulatory functions represent key immune elements. Given their reciprocal interdependency lymphocyte subpopulations are usually assayed as diagnostic panels, rather than single biomarkers for specialist clinical use. This retrospective analysis on lymphocyte subpopulations, analyzed over the last few years in an outpatient laboratory in Northeast Italy, contributes to the establishment of reference values for several regulatory lymphocytes currently lacking such reference ranges for the general population. Mean values and ranges in a sample of Caucasian patients (mean age 42±8,5 years), were provided for Th1, Th2, Th17, Th-reg, Tc-reg, Tc-CD57+ and B1 lymphocytes. The results are consistent with what is found in literature for the single subtypes and are: Th1 157.8±60.3/ml (7.3%±2.9); Th2 118.2±52.2/ml (5.4%±2.5); Th17 221.6±90.2/ml (10.5%±4.4); Th-reg 15.1±10.2/ml (0.7%±0.4); Tc-reg 5.8±4.7/ml (0.3%±0.2); Tc-CD57+ 103.7±114.1/ml (4.6%±4.7); B1 33.7±22.8/ml (1.5%±0.9); (Values are mean±SD). The results show that despite their variability, mean values are rather consistent in all age or sex groups and can be used as laboratory internal reference for this regulatory panel. Adding regulatory cells to lymphocyte subpopulations panels allows a more complete view of the state of the subject’s immune network balance, thus improving the personalization and the “actionability” of diagnostic data in a systems medicine perspective.

Scalable production of tissue-like vascularized liver organoids from human PSCs

Harrison Sean P.,Siller Richard,Tanaka Yoshiaki,Chollet Maria Eugenia,de la Morena-Barrio María Eugenia,Xiang Yangfei,Patterson Benjamin,Andersen Elisabeth,Bravo-Pérez Carlos,Kempf Henning,Åsrud Kathr 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes, rendering them less physiologically relevant compared to native tissue. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. Using single-cell RNA sequencing and immunofluorescence, we demonstrate a liver-like cellular repertoire, a higher order cellular complexity, presenting with vascular luminal structures, and a population of resident macrophages: Kupffer cells. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality. The organoids can be transplanted and maintained long term in mice producing human albumin. The organoids exhibit a complex cellular repertoire reflective of the organ and have de novo vascularization and liver-like function. These characteristics are a prerequisite for many applications from cellular therapy, tissue engineering, drug toxicity assessment, and disease modeling to basic developmental biology.