Effect of acute pentylenetetrazol injection induced epileptic seizures on rat dentate gyrus at different postnatal ages

Heba K,Mohamed1,Sohair A,Eltony 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.1

Epilepsy is one of the most common neurological disorders, its prevalence approximately from 0.5% to 2% of the general population. Generalized seizures could lead to several morphological changes in the brain. This study aimed to investigate the morphological effects of a single convulsive dose of pentylenetetrazol (PTZ) on rat dentate gyrus at different postnatal ages. Thirty-six male Wistar rats were used at the following postnatal ages: P10, P21, and P90 (12 rats per each age). The animals in each age were equally divided into two groups: group I, control and group II, treated with a single intraperitoneal injection of PTZ (55 mg/kg). After confirmation of generalized tonic-clonic seizures, specimens from the right dentate gyrus were processed for light and electron microscopy. In PTZ-treated groups, the number of granule cells significantly decreased. Dark granule cells appeared in the deep layers of the granule cells in P10 and with the progress of age, they significantly increased in number and extended into the superficial layers of the granule cells. The dendritic spines diminished. Glial fibrillary acidic protein and caspase-3 expression increased. Ultrastructurally, granule cells showed irregular shaped nucleus, dilated rough endoplasmic reticulum (RER) cisternae, mitochondria with damaged cristae, large vacuoles, lysosomes, and lipofuscin granules. Dark granule cells characterized by electron-dense nucleus and cytoplasm containing disorganized Golgi bodies, swollen mitochondria with damaged cristae, numerous free ribosomes and few long strands of RER. Astrocytes had hypertrophied cell body. Acute treatment with PTZ-induced epileptic seizures caused toxic effect on the structure of rat dentate gyrus at different postnatal ages.

아랍권 한국어 학습자 음운 규칙의 적용 양상 분석 - 중급 및 고급수준의 이집트인 한국어 학습자를 중심으로 -

헤바무함메드 ( Heba Mohammed ) 연세대학교 언어정보연구원(구 연세대학교 언어정보개발원) 2016 언어사실과 관점 Vol.39 No.-

This research aims to analyze and compare phonological rules mistakes which are mistakes made by middle level and high level Arab students who are studying Korean language in Egypt. 14 middle level students and same number of heigh level students were chosen to participate in this research. The unique phonological rules is one of the reasons that make Korean language pronunciation difficult. To determine problems facing Arab students when pronouncing Korean 64 words were given to middle·heigh level Arab students and the reading process has been recorded, and then the students were asked to write the pronunciation to confirm the students level of knowledge of these phonological rules. 3 of the phonological rules were chosen to be the subject of analyzing in this research fortis, Aspirated consonant, palatalization. Then the applyment of the rules to every word was analyzed to applied, not applied, misapplied. Both middle·Heigh level students show high applyment of palatalization, middle students face problems is understanding the rule itself, and that`s why they show heigh “miss applied” rate, but this problem was solved in the heigh students group so the “apply” rate moved to 80% from 54%. fortis “apply” rate 39% was the worst between middle level students to show that fortis is the most difficult rule among the chosen 3 rules for the middle level students, but heigh level students show more high “apply” rate 52%. heigh level students fortis pronunciation was improved but the still show heigh rate of “not applied” and “misapplied” rate 48% which is heigh rate and shows that also heigh level students face real problems appling fortis phonological rule. Aspirated consonant shows interesting result as the apply rate in both middle students group and heigh students group was same, which means Aspirated consonant of heigh level students was not going to improve at all. According to the result of this research the most difficult phonological rule for arab learners among the 3 rules were chosen to be analyzed in this research is Aspirated consonant which was pronunciated as fortis most of time. and the reason behind this is the mother language of the learners as the arab learners can not divide both pronunciation from each other. at the end of the research pronunciation teaching methods were suggested to solve this problem among korean language arab learner specially in the korean department of EGYPT.

Sustainable super fast adsorptive removal of Congo red dye from water by a novel technique based on microwave-enforced sorption process

Mohamed E. Mahmoud,Azza E.H. Abdou,Aziza K. Shehata,Heba M.A. Header,Ezzat A. Hamed 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-

Removal of Congo red was completed in 5–20 s using microwave-enforced sorption (MES) as a sustainable technique. Nano-γ-Al2O3 was covalently bonded with 3-chloropropyltrimethoxysilane to produce γ-Al2O3-SiCl nanosorbent via microwave-assisted approach. Congo red extraction by γ-Al2O3 and γ-Al2O3-SiCl were found 58.42% and 69.41% and increased to 66.13% and 81.47%, respectively using 5 and 20 s of microwave heating. The percentage extraction of Congo red (30 mg L−1) by γ-Al2O3-SiCl was increased from 46.21% to 83.61% upon increasing the pH from 1.0 to 10.0. Other controlling conditions were investigated and optimized. Excellent recovery of Congo red from wastewater was established in 20 s.

Kinetics, isotherm, and thermodynamic studies of the adsorption of reactive red 195 A dye from water by modified Switchgrass Biochar adsorbent

Mohamed E. Mahmoud,Gehan M. Nabil,Nabila M. El-Mallah,Heba I. Bassiouny,Sandeep Kumar,Tarek M. Abdel-Fattah 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.37 No.-

A method is described for efficient decolorization of reactive red 195 A dye (RR-195A) from aqueous andwastewater samples using modified Switchgrass Biochar (SB) by tetradecyltrimethyl ammoniumbromide (TTAB) as a cationic surfactant for the formation of a novel SB-TTAB adsorbent. The differentcontrolling experimental conditions such as solution pH, initial dye concentration, contact time,adsorbent mass, interfering ions and temperature have been investigated and optimized. Adsorptionequilibrium using Langmuir, Freundlich, Temkin and Dubinin–Radushkevich (D–R) isotherm modelshave been also studied and the best fit was related to the Langmuir adsorption isotherm. Adsorptionkinetics have been tested and illustrated using pseudo first-order, pseudo-second-order, intraparticlediffusion and Elovich models. The collected results confirmed that the pseudo-second order kineticmodel exhibited the best fit with the experimental data. Thermodynamic parameters were calculatedand the results referred to an exothermic nature and spontaneity of the adsorption process. Improveddecolorization of reactive red 195 A dye from textile industrial wastewater and other water sampleswere successfully established. Excellent recovery values of RR-195A dye by SB-TTAB adsorbent weredetermined in the ranges of 96.61–98.76%, 97.79–99.26%, 94.24–100.0% and 90.98–92.96% ( 1.0–4.0%)from tap water, raw water, wastewater and sea water, respectively

Silymarin Modulates Cisplatin-Induced Oxidative Stress and Hepatotoxicity in Rats

( Heba Hosny Mansour ),( Hafez Farouk Hafez ),( Nadia Mohamed Fahmy ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.6

Effect of implant diameter and cantilever length on the marginal bone height changes and stability of implants supporting screw retained prostheses: A randomized double blinded control trial

Heba Ezzeldin Khorshid,Noha Ossama Issa,Amr Mohamed Ekram 대한치과보철학회 2023 The Journal of Advanced Prosthodontics Vol.15 No.3

PURPOSE. This randomized controlled trial aimed to evaluate the effect of implants’ two different diameters and cantilever lengths on the marginal bone loss and stability of mplants supporting maxillary prostheses. MATERIALS AND METHODS. Ninety-six implants were placed in sixteen completely edentulous maxillary ridges. Patients were randomly divided into two groups: Group A, implants were placed with a cantilever to anterior-posterior AP spread length (CL:AP) at a ratio of 1:3; Group B, implants were placed with a CL:AP at a ratio of 1:2. Patients were further divided into four sub-groups: Groups A1, A2, B1, and B2. Groups A1 and B1 received small diameter implants while Groups A2 and B2 received standard diameter implants. Bone height and stability measurements around each implant were performed at 0, 4, 8 and 24 months after definitive prostheses delivery. RESULTS. Statistical analysis of the mean implant stability and height values revealed an insignificant difference between Group A1 and Group A2 at all the different time intervals while significantly higher values in Group B1 in comparison with Group B2. Results also showed significantly higher values in Group A1 in comparison with Group B1 and an insignificant difference between Group A2 and Group B2 at all the different time intervals. CONCLUSION. It can be concluded that the use of small diameter implants placed with a CL:AP at a ratio of 1:3 provided predictable results and that the 1:2 CL:AP significantly induced more critical bone loss in the small diameter implants group, which can significantly reduce long term success and survival of implants.

The impact of omeprazole on mycophenolate pharmacokinetics in kidney transplant recipients

( Mohamed S. Abdelhalim ),( Ahmed S. Kenawy ),( Heba H. El Demellawy ),( Amany A. Azouz ),( Sarah S. Alghanem ),( Torki Al-otaibi ),( Osama Gheith ),( Mohamed Abd Elmonem ),( Mohammed K. Afifi ),( Rag 대한신장학회 2020 Kidney Research and Clinical Practice Vol.39 No.4

Background: The absorption rates of mycophenolate mofetil (MMF) and enteric-coated mycophenolate sodium (ECMPS) may be influenced by the concomitant use of omeprazole. Methods: One hundred kidney transplant patients were recruited during their outpatient visits, including 50 on MMF and 50 on EC-MPS. At the clinic, a predose mycophenolic acid (MPA) sample (C0) was collected; subsequently, the participants received the proton-pump inhibitor omeprazole along with either MMF or EC-MPS. Two more blood samples were collected at 1.5 and 3.5 hours and used to estimate an area under the curve (AUC) from zero to 12 hours [AUC (0-12)]. Results: The mean number of months after transplant was 92 months. The median AUC (0-12) and C0 results were 62.2 mg·h/L and 2.0 mg/L for the MMF group and 71.9 mg·h/L and 1.8 mg/L for the EC-MPS group (P = 0.160 and 0.225, respectively). Interestingly, 54% of the MMF group and 62% of the EC-MPS group showed AUCs above the target values. The correlation between MPA C0 and the predicted AUC was poor in both groups. Conclusion: Omeprazole can be safely co-administered with either MMF or EC-MPS, as it did not compromise the MPA exposure. Unexpectedly, however, a high percentage of patients presented MPA AUCs exceeding the target value, highlighting the importance of periodically assessing MPA level.

Resveratrol: Isolation, and Its Nanostructured Lipid Carriers, Inhibits Cell Proliferation, Induces Cell Apoptosis in Certain Human Cell Lines Carcinoma and Exerts Protective Effect Against Paraquat-Induced Hepatotoxicity

Heba A. Elgizawy,Ali A. Ali,Mohammed A. Hussein 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.1

Resveratrol (RES) (trans-3, 5,-4′-trihydroxystilebene) is a multi-biofunctional compound found in a variety of plants such as grapes and mulberries. Studies of nanoencapsulated resveratrol have indicated that this compound can inhibit the growth of cancer cells and free radicals. The aim of this study was to isolate resveratrol from Vitis vinifera, develop and evaluate resveratrol nanostructured lipid carriers (NLCs) and/or resveratrol encapsulated chitosan-coated nanostructured lipid carriers (CSNLCs) using low-viscous chitosan for anticancer therapy. In addition, our study was carried out to examine the prophylactic potential of RES, NLC, and CSNLC on paraquat-induced injury in rat hepatocytes. In this study we isolated resveratrol and encapsulated NLCs in phosphate-buffered saline solution using a phase inversion method. In addition, CSNLCs were prepared by ionic gelation method of NLCs using chitosan. NLCs and CSNLCs were then characterized for their particle size, zeta potential, morphology, and entrapment efficiency. Furthermore, NLCs and CSNLCs were evaluated for their cytotoxic effect on Hep-G2, human HCT-116 (colorectal cancer cell line), lymphoblastic leukemia (1301), and human MCF-7 (Michigan Cancer Foundation-7) cells as well as their effect on caspase-3 and death receptor (DR-4). In addition, incubation of hepatocytes with paraquat resulted in increased formation of TBARS (thiobarbituric acid reactive substances) with a parallel increase in lactate dehydrogenase (LDH) leakage at 1 h after incubation. Time-dependent depletion of cellular glutathione (GSH) was observed starting 2 h after incubation with paraquat. The mean particle size of NLC and CSNLC were 67.0 and 98.41 nm, zeta potential were (−) 24.8 and (+) 31.6 mV, entrapment efficiency were 74.15% and 85.46%, respectively, with the observed shapes of nanoparticle being spherical. The treatment of Hep-G2, human HCT-116, lymphoblastic leukemia (1301), and human MCF-7 cells with NLC led to high inhibition in the cell proliferation as concluded by the low IC50 values 27.7, 17.43, 35.39, and 47.66 μg/mL, respectively, whereas CSNLC had high cytotoxic effect on Hep-G2, human HCT-116, lymphoblastic leukemia (1301), and human MCF-7 cells with low IC50 values 13.29, 10.56, 16.79 and 22.60 μg/mL, respectively. Both NLC and CSNLC possess apoptotic properties through activation of the caspase-3 and death receptor (DR-4). In addition, incubation of hepatocytes with RES, NLC, and CSNLC markedly protected against paraquat-induced formation of TBARS, increase in LDH leakage, and prevented GSH depletion. The most effective doses for ethyl acetate, ethanolic, and aqueous extracts were 7.5, 10, and 12.5 μg, respectively. The results presented here may suggest that nanoencapsulated resveratrol isolated from the stems of V. vinifera to obtain NLC and CSNLC possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as new approach of pharmaceutical drugs. In addition, the results clearly suggest that the RES, NLC, and CSNLC exerted protective effect against cytotoxicity induced by paraquat. On the contrary, the effect decreased in order of CSNLC, NLC, and RES.

Silymarin Modulates Cisplatin-Induced Oxidative Stress and Hepatotoxicity in Rats

Mansour, Heba Hosny,Hafez, Hafez Farouk,Fahmy, Nadia Mohamed Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6

Cisplatin (CDDP) is a widely used anticancer drug, but at high dose, it can produce undesirable side effects such as hepatotoxicity. Because silymrin has been used to treat liver disorders, the protective effect of silymarin on CDDP -induced hepatotoxicity was evaluated in rats. Hepatotoxicity was determined by changes in serum alanine aminotransferase [ALT] and aspartate aminotransferase [AST], nitric oxide [NO] levels, albumin and calcium levels, and superoxide dismutase [SOD], glutathione peroxidase [GSHPx] activities, glutathione content, malondialdehyde [MDA] and nitric oxide [NO] levels in liver tissue of rats. Male albino rats were divided into four groups, 10 rats in each. In the control group, rats were injected i.p. with 0.2 ml of propylene glycol in saline 75/25 (v/v) for 5 consecutive days [Silymarin was dissolved in 0.2 ml of propylene glycol in saline 75/25 v/v]. The second group were injected with CDDP (7.5 mg /kg, I.P.), whereas animals in the third group were i.p. injected with silymarin at a dose of 100 mg/kg/day for 5 consecutive days. The Fourth group received a daily i.p. injection of silymarin (100 mg/kg/day for 5 days) 1 hr before a single i.p. injection of CDDP (7.5 mg/kg). CDDP hepatotoxicity was manifested biochemically by an increase in serum ALT and AST, elevation of MDA and NO in liver tissues as well as a decrease in GSH and the activities of antioxidant enzymes, including SOD, GSHPx in liver tissues. In addition, marked decrease in serum NO, albumin and calcium levels were observed. Serum ALT, AST, liver NO level, MDA was found to decreased in the combination group in comparison with the CDDP group. The activities of SOD, GSHPx, GSH and serum NO were lower in CDDP group than both the control and CDDP pretreated with silymarin groups. The results obtained suggested that silymarin significantly attenuated the hepatotoxicity as an indirect target of CDDP in an animal model of CDDP-induced nephrotoxicity.

Yarrow oil ameliorates ulcerative colitis in mice model via regulating the NF-κB and PPAR-γ pathways

( Maged E. Mohamed ),( Sahar A. Elsayed ),( Hafez R. Madkor ),( Heba M. Saad Eldien ),( Omar M. Mohafez ) 대한장연구학회 2021 Intestinal Research Vol.19 No.2

Background/Aims: Ulcerative colitis (UC) is a chronic inflammatory disorder with indefinite etiology; however, environmen-tal, genetic, immune factors and microbial agents could be implicated in its pathogenesis. UC treatment is lifelong, therefore; the potential side effects and cost of the therapy are significant. Yarrow is a promising medicinal plant with the ability to treat many disorders, owing to its bioactive compounds especially the essential oil. The main aim of this research was to investigate the therapeutic effect of the yarrow oil on colitis including the involved mechanism of action. Methods: In 21-female C57BL/6 mice were divided into 3 groups; control group, colitis model group, and oil-treated group. Groups 2 and 3 received 5% dextran sulfate sodium (DSS) in drinking water for 9 days, and concomitantly, only group 3 was given 100 mg/kg yarrow oil. Mice were examined for their body weight, stool consistency and bleeding, and the disease activity indexes were calculated. Results: Oral administration of yarrow oil markedly repressed the severity of UC via the reduction of the inflammatory signs and restoring colon length. The oil was able to down-regulate nuclear factor kappa light chain enhancer of activated B cells (NF-κB), up-regulate peroxisome proliferator-activated receptor gamma (PPAR-γ), and enhance transforming growth factor-β expression. The oil normalized the tumor necrosis factor-α expression, restored the normal serum level of interleukin-10 (IL-10) and reduced the serum level of IL-6. Conclusions: Yarrow oil mitigated UC symptoms and regulated the inflammatory cytokines secretion via regulation of NF-κB and PPAR-γ pathways in the mice model, however, this recommendation requires further investigations using clinical studies to confirm the use of the oil on humans. (Intest Res 2021;19:194-205)