운동 직후 고온 침수가 EPOC와 TG/ fatty acid cycling에 미치는 영향

조현철,김종규,강민철,홍완표,박노혁 龍仁大學校 體育科學硏究所 2005 體育科學硏究論叢 Vol.15 No.1

The purpose this study was to estimate effects of EPOC and TG/fatty acid cycling on warm water immersion of immediately after exercise. To elucidate the role of fatty metabolism, a sequence of five experiments was performed. Seven physically active, male subjects volunteered to participate in the presented study. The mean values for age, body mass and hight were 25±1.52 yr, 79.2±9.52kg, 177.2±4.62cm, respectively. After giving consent, participant visited the laboratory on six occasion: 1) 30min of treadmill exercise VO2max 55% and a further 60min recovery, 2) 30min partial body warm water immersion in a 39℃ and a further 60min recovery, 3) 30min whole body warm water immersion in a 39℃ and a further 60min recovery, 4) 30min of treadmill exercise VO2mas 55% and in 30min partial body warm water immersion in a 39℃, 5) 30min of treadmill exercise VO2max 55% and in 30min whole body warm water immersion in a 39℃. When compared by recover period within repeat on the base of changes of subjects average body temperature, not effective interactions among repeat. However, partial and whole body warm water immersion immediately after exercise shows it as the best effective exercise for VO2max 55%, partial and whole body warm water immersion. When compared by recover period within repeat on the base of changes of subjects EPOC, effective interactions among repeat(p<.05). Partial and whole body warm water immersion immediately after exercise shows it as the best effective exercise for VO2mas 55%, partial and whole body warm water immersion. The catecholamines concentration was significantly higher partial and whole body warm water immersion than exercise of VO2max 55%(p<.05). The TG concentration and free fatty acid was significantly higher partial and whole body warm water immersion immediately after exercise than exercise of VO2max 55% than Partial and whole body warm water immersion(p<.05). Based on the facts that we have discussed above, human metabolism is increased by both exercise and conditions of immersion and partial and whole body warm water immersion immediately after exercise than exercise of VO2maw 55% shows it as better effective treatment for increasing TG/Fatty acid cycling activation. Due to extremely heavy stress complained by subjects during whole body immersion, it is thought that more researches on it should be required.

Antioxidant Activity and Whitening Effect of Lindera erythrocarpa Extract

Min ?Chul Kang,Ryeo Kyeoung Ko,Ju-Yeop Lee,Haeng-Bum Kim,Bong-Seok Kim,Jong-Heon Han,Seong-Hyun An,Gi-Ok Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

식품중 중금속 규격 과학화를 위한 조사연구(Ⅲ) : 음료수의 중금속 향량에 대하여 Heavy metal contents beverages

김명철,김미혜,권기성,정소영,박성국,이윤동,이승훈,김은정,강민철,박효정 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 국내에 유통되고 있는 음료류중 중금속 함량을 파악하여 식품공전 제 ·개정시 과학적 기초자료로 활용하고자 수행하였다. 출 2☞7여건의 음료류에 대하여 유도결합플라스마 분광기(ICP), 원자홉광광도 계(AAS) 및 수은분석기 등을 치용하여 납, 카드윰 등 중금속함량을 측정하였으며 그 결과는 다음과 같다 [단위 : min-ma)t(irran) ; mrk3 ; f : 0.0001-0.0025(0.0003), As :불검출곰.OB45(0.0003), Pb : 불검출-0.0893(0.0041),Cd ' 불검출곤.0064(0.0011), Cfl : 불검출.(i9(0.05), Mn : 불검출글.찌(0.16), Zn : 불검출-12.85(0.뽀), Sn :불검출-45.36(1.97). 본 조사결과 우리나라에서 유통되고 있는 음료류 중 납 등 중금속 함량은 외국의 모니터랑 결과와 유사하였다. 또한 우리나라 국민이 음료류를 통한 중금속 섭취량은 FAO/UTfO에서 설정한 잠 정주간섭취처용량의 약 0.OleO.06% 이하로 매우 낮았다. 편 연구조사에서 먼어진 결과는 음료류 중 납 기준 설정을 위한 중요한 기초자료로 활용할 수 있는 것으로 사료된다. This study was conducted to estimate the cantents of heavy netals in beverages and to fIFovide a scientific basis for heav)· meta3 standardizatior of Korea Food Code. The contents of rriercur17(Hg), lead(Pb), cadmium(Cd), arsenic(As), manganese(Mn),ziac(Zn), tin(Sn) and topper(Cu) were determined in a total of 20? samples of beverages usinga mercury anal!'zer, aromic absorption spectrophotometer(AAS) and inductiuety coupled plasmaspectrometer(fcpl. The vatues of heavy metals In beverages were as fellows (min-max (mean) , mg/krl , Hg : 0.DOOI-0.0095(0.0004), As : ND-0.0245(0.0003), Pb ND-0.0893(0.0041), Cd : ND-0.0064(0.0011),Cu : ND-1.69(0.03), Mn : ND-3.94(0.16), Zn ND-12.85(0.28), Sn : ND-45.36(1,97). Our results weresimilar to thofe reported by other countries. It is thought that our results could be utilizedimportant references toT es?ablish the standard of lead in beverages.

Involvement of calcium-mediated apoptotic signals in H₂O₂-induced MIN6N8a cell death

Choi, Sung-E,Min, Se-Hee,Shin, Ha-Chul,Kim, Hyo-Eun,Jung, Min Whan,Kang, Yup Elsevier 2006 european journal of pharmacology Vol.547 No.1

<P><B>Abstract</B></P><P>Reactive oxygen species are believed to be the central mediators of beta-cell destruction that leads to type 1 and 2 diabetes, and calcium has been reported to be an important mediator of beta cell death. In the present study, the authors investigated whether Ca<SUP>2+</SUP> plays a role in hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-induced MIN6N8a mouse beta cell death. Treatment with low concentration H<SUB>2</SUB>O<SUB>2</SUB> (50?μM) was found to be sufficient to reduce MIN6N8a cell viability by 55%, largely via apoptosis. However, this H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death was near completely blocked by pretreatment with BAPTA/AM (5?μM), a chelator of intracellular Ca<SUP>2+</SUP>. Moreover, the intracellular calcium store channel blockers, such as, xestospongin c and ryanodine, significant protected cells from 50?μM H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death and under extracellular Ca<SUP>2+</SUP>-free conditions, 50?μM H<SUB>2</SUB>O<SUB>2</SUB> elicited transient [Ca<SUP>2+</SUP>]<SUB>i</SUB> increases. In addition, pharmacologic inhibitors of calpain, calcineurin, and calcium/calmodulin-dependent protein kinase II were found to have a protective effect on H<SUB>2</SUB>O<SUB>2</SUB>-induced death. Moreover, H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptotic signals, such as c-JUN N-terminal kinase activation, cytochrome <I>c</I> release, caspase 3 activation, and poly (ADP-ribose) polymerase cleavage were all down-regulated by the intracellular Ca<SUP>2+</SUP> chelation. These findings show that [Ca<SUP>2+</SUP>]<SUB>i</SUB> elevation, possibly due to release from intracellular calcium stores and the subsequent activation of Ca<SUP>2+</SUP>-mediated apoptotic signals, critically mediates low concentration H<SUB>2</SUB>O<SUB>2</SUB>-induced MIN6N8a cell death. These findings suggest that a breakdown of calcium homeostasis by low level of reactive oxygen species may be involved in beta cell destruction during diabetes development.</P>

벤조디아제핀 수용체 영상용 양전자 방출 핵종 표지 플루마제닐 유도체 [F-18](3-(2-Fluoro)flumazenil의 합성과 생체 내 분포

장영수,이숙자,강삼식,홍성현,이명철,이동수,정준기,정재민,조정혁 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.6

Purpose: Radiotracers that bind to the central benzodiazepine receptor are useful for the investigation of various neurological and psychiatric diseases. [C-11]Flumazenil, a benzodiazepine antagonist, is the most widely used radioligand for central benzodiazepine receptor imaging by PET. We synthesized 3-(2-[F- 18]fluoro)flumazenil, a new fluorine-18 (t1/2=110 min) labeled analogue of benzodiazepine receptor imaging agent, and evaluated in vivo for biodistribution in mice. Materials and Methods: Flumazenil (Ro 15-1788) was synthesized by a modification of the reported method. Precursor of 3-(2-[F-18]fluoro)flumazenil, the tosylated flumazenil derivative was prepared by the tosylation of the ethyl ester by ditosylethane. [F-18] labeling of tosyl substitued flumazenil precursor was performed by adding F-18 ion at 85℃ in the hot cell for 20 min. The reaction mixture was trapped by C18 cartridge, washed with 10% ethanol, and eluted by 40% ethanol. Bidistribution in mice was determined after intravenous injection Results: The total chemical yield of tosylated flumazenil derivative was ∼40%. The efficiency of labeling 3-(2-[F-18]fluoro)flumazenil was 66% with a total synthesis time of 50 min. Brain uptakes of 3-(2-[F-18]fluoro)flumazenil at 10, 30, 60 min after injection, were 2.5±0.37, 2.2±0.26, 2.1±0.11 and blood activities were 3.7±0.43, 3.3±0.07, 3.3±0.09%ID/g, respectively. Conclusion: We synthesized a tosylated flumazenil derivative which was successfully labeled with no-carrier-added F-18 by nucleophilic substitution.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme

Kang, Wonchull,Hong, Se Hoon,Lee, Hye Min,Kim, Na Yeon,Lim, Yun Chan,Le, Le Thi My,Lim, Bitna,Kim, Hyun Chul,Kim, Tae Yeon,Ashida, Hiroki,Yokota, Akiho,Hah, Sang Soo,Chun, Keun Ho,Jung, Yong-Keun,Yang National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.1

<P>APIP, Apaf-1 interacting protein, has been known to inhibit two main types of programmed cell death, apoptosis and pyroptosis, and was recently found to be associated with cancers and inflammatory diseases. Distinct from its inhibitory role in cell death, APIP was also shown to act as a 5-methylthioribulose-1-phosphate dehydratase, or MtnB, in the methionine salvage pathway. Here we report the structural and enzymatic characterization of human APIP as an MtnB enzyme with a <I>K</I><SUB><I>m</I></SUB> of 9.32 μM and a <I>V</I><SUB><I>max</I></SUB> of 1.39 μmol min<SUP>−1</SUP> mg<SUP>−1</SUP>. The crystal structure was determined at 2.0-Å resolution, revealing an overall fold similar to members of the zinc-dependent class II aldolase family. APIP/MtnB exists as a tetramer in solution and exhibits an assembly with <I>C4</I> symmetry in the crystal lattice. The pocket-shaped active site is located at the end of a long cleft between two adjacent subunits. We propose an enzymatic reaction mechanism involving Glu139* as a catalytic acid/base, as supported by enzymatic assay, substrate-docking study, and sequence conservation analysis. We explored the relationship between two distinct functions of APIP/MtnB, cell death inhibition, and methionine salvage, by measuring the ability of enzymatic mutants to inhibit cell death, and determined that APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis. Our results establish the structural and biochemical groundwork for future mechanistic studies of the role of APIP/MtnB in modulating cell death and inflammation and in the development of related diseases.</P>

Tegretol-CR의 抗 躁症 효과에 관한 임상적 연구

강민희,왕성근,신석철 최신의학사 1993 最新醫學 Vol.36 No.7

개인휴대통신의 기술발전

강민구,조형래,강성철 호남대학교 정보통신연구소 1994 정보통신연구 Vol.3 No.-

이동통신 서비스의 증가와 무선통신 기술의 발전으로 개인휴대통신 서비스의 도입이 각국에서 활발히 추진되고 있다. 본 논문은 국내 개인휴대통신 서비스의 보급과 확대를 위해 고려해야 할 여러 시장 동향과 표준화 동향에 대해 조사하고, 개인휴대통신 구축을 위한 기반기술인 지능형 교환기와 부호다중화 접속기술 및 개인휴대통신 서비스의 직면한 과제의 해결책에 논한다. In this thesis, the technical trend of personal communication service (PCS) is showned for the implementation of PCS in Korea. The comparision betweem technical schemes of many countries is represented for the Korean standards and the core technique of PCS. EspEcially, the code division multiple access (CDMA) and the intelegent switching systems are explained for the Korean PCS standards.

PDA를 이용한 건설 현장업무의 효율 제고를 위한 연구

강민수,신규철,김재준 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

Information Technology is making rapid progress in other industries. However, construction industry is composed of various teams spreaded out to work in their place, so supply and demand on construction information within the appropriate time and the right place are becoming more important and control a successful completion of the construction project. Many researches have been conducted and operated in various construction work scope such as productivity management, logistics management, and so on. However the research concerned work scope that can be efficiently managed by using IT devices are insufficient. This study investigates how this technology especially PDA can be used to improve efficiency and effectiveness of construction management process. It classifies work scope into 9 catalogs based on PMBOK, and subdivides into 28 catalogs. This study propose the future works on PDA application in construction industry from the improvement effect's and applicability's point of view.