LAMC1 expression patterns and associated clinical features of endometrial cancer

( Haruko Irie-kunitomi ),( Yusuke Kobayashi ),( Kouji Banno ),( Kenta Masuda ),( Megumi Yanokura ),( Eichiro Tominaga ),( Daisuke Aoki ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

Objective: Laminins are one of the major components of the extracellular matrix. Recent studies have indicated the relationship between specific laminin expression profiles and cancer progression. Upregulation of LAMC1, which encodes laminin-γ1, is of particular note because this is related to aggressive behavior of cancer cells of various origins. This study aimed to analyze the influence of LAMC1 expression on the clinical features of endometrial cancer. Methods: The subjects were patients with endometrial cancer (EC), atypical endometrial hyperplasia complex (AEHC) or normal endometrium (NE) who underwent hysterectomy during January 2008 and December 2011 at our institute. The study included 100 cases of EC, 9 of AEHC, and 16 NE. LAMC1 immunohistochemical staining was evaluated as negative or positive by two independent evaluators blinded from clinical information. Patient characteristics and clinical outcomes were obtained retrospectively from clinical records. This study was approved by our institutional review board. Results: LAMC1 expression was significantly elevated in EC compared to AEHC and NE (p<0.05). Endometrioid (EM) grade 3, serous and clear cell adenocarcinoma indicated frequent LAMC1 expression compared to EM grade 1 and 2 (85.2% vs. 51.3%, p<0.05). Patients with advanced FIGO surgical stage (stage III and IV) and positive lymphovascular space invasion had a significantly higher LAMC1 expression rate compared to early stage cases (83.7% vs. 63.2%, p<0.05) and cases without lymphovascular space invasion (86.3% vs. 61.0%, p<0.05), respectively. Kaplan-Meier analysis revealed that LAMC1 positive cases had a significantly shorter progression-free survival (p<0.05) and a tendency to have a reduced overall survival (p=0.052). Conclusion: In endometrial cancer, LAMC1 expression is related to aggressive tumor behavior and reduced progression free survival.

Screening for Lynch syndrome using risk assessment criteria in patients with ovarian cancer

Takashi Takeda,Kosuke Tsuji,Kouji Banno,Megumi Yanokura,Yusuke Kobayashi,Eiichiro Tominaga,Daisuke Aoki 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.3

Objective: Lynch syndrome is a cancer predisposition syndrome caused by germline mutation of DNA mismatch repair (MMR) genes. Lynch syndrome only causes about 0.4% of cases of ovarian cancer, which suggests that universal screening may not be cost-efficient. However, the frequency of Lynch syndrome in ovarian cancer is unclear in the Asian population. The goal of the study was to investigate a screening strategy using family history. Methods: The subjects were 129 patients with ovarian cancer. Clinical and family history were collected using a self-administered questionnaire, and Society of Gynecologic Oncology (SGO) criteria 2007 and PREMM5 were used for risk assessment. Microsatellite instability, immunohistochemistry, and methylation of MMR genes were analyzed. Results: Of the 129 cases, 25 (19.4%) met the SGO criteria, and 4 of these 25 had MSI-high and MMR deficiency. Two cases had loss of MSH2 and MSH6, indicating MSH2 mutation, and the other two had loss of MLH1 and PMS2, including one without MLH1 methylation indicating MLH1 mutation. These results show that screening using family history can detect Lynch syndrome in 12.0% (3/25) of ovarian cancer cases. The 3 cases were positive for PREMM5, but negative for Amsterdam II criteria and revised Bethesda guidelines. Genetic testing in one case with MSH2 and MSH6 deficiency confirmed the diagnosis of Lynch syndrome with MSH2 mutation. Conclusion: This is the first study of screening for Lynch syndrome in ovarian cancer using clinical and family history in an Asian population. This approach may be effective for diagnosis in these patients.

Long-term Embryogenic Callus Cultures as a Source of Tetraploid Plants in the Liliaceous Ornamental Tricyrtis hirta

Junji Amano,Toshikazu Nomizu,Keiko Mizunashi,Mami Suzuki,Shiro Mori,Sachiko Kuwayama,Megumi Hayashi,Hiroto Umehara,Eriko Oka,Hiroyuki Kobayashi,Megumi Asano,Shintaro Sugawara,Hiroki Takagi,Yoshihiro H 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Portal bifurcation reconstruction using own hepatic vein grafts due to portal vein anomaly of the living donor for the patient with portal vein thrombosis

Akira Umemura,Hiroyuki Nitta,Takeshi Takahara,Yasushi Hasegawa,Hirokatsu Katagiri,Shoji Kanno,Megumi Kobayashi,Taro Ando,Taku Kimura,Akira Sasaki 한국간담췌외과학회 2020 Annals of hepato-biliary-pancreatic surgery Vol.24 No.4

A 57-year-old Japanese female was considered for living donor liver transplantation (LDLT) due to end-stage liver cirrhosis caused by primary biliary cholangitis with portal vein thrombosis (PVT) formation. A 26-year-old daughter of the patient was selected as a living donor; however, a computed tomography examination revealed trifurcated-type portal vein anomaly (PVA). Preoperative liver volumetry showed that the right lobe graft was necessary for the recipient; therefore, reconstruction of the portal vein bifurcation during LDLT was necessary. We planned to extract the recipient’s own hepatic vein grafts after total hepatectomy, and these would be attached with anterior and posterior portal branches as jump grafts. We performed laparoscopic donor hepatectomy as usual, and the recipient’s hepatic vein grafts were anastomosed on the bench. Then, the liver graft was inserted, and the hepatic vein reconstruction was routinely performed. We confirmed the alignment between the recipient’s portal vein and the bridged hepatic vein graft of the liver graft’s posterior branch, and anastomosed these two vessels. Moreover, we confirmed the front flow and expansion of the reconstructed posterior branch by declamping only the suprapancreatic side of the portal vein. The decision regarding the punch-out location was crucial. We confirmed the alignment between the reconstructed posterior branch and the bridged hepatic vein graft of the anterior branch, and anastomosed these two vessels employing the punched-out technique. In LDLT, liver transplant surgeons occasionally encounter living donors with PVA or recipients with PVT. Our contrivance may be useful when the liver graft needs reconstruction of portal vein bifurcation.

Virulence Determinants in Vancomycin-Resistant Enterococcus faecium vanA Isolated from Different Sources at University Hospital of Londrina, Paraná, Brazil

Flávia Imanishi Ruzon,Suelen Balero de Paula,Renata Lumi Kanoshiki,Jussevania Pereira-Santos,Gilselena Kerbauy,Renata Katsuko Takayama Kobayashi,Lucy Megumi Yamauchi,Márcia Regina Eches Perugini,Sueli 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

Enterococcus faecium, especially those showing multidrug resistance, has emerged as a significant cause of healthcare-associated infections worldwide. However, relatively little is known about the virulence and pathogenesis of this species. The aim of this study was to determine the occurrence of four putative virulence determinants of E. faecium and to correlate them with phenotypic traits. Using forty E. faecium vanA-type isolates from hospitalized patients and their environmental vicinity, we determined the following: the antimicrobial susceptibility profile, occurrence of the genes cylA, efaA, esp, and gelE, hemolytic and gelatinase activities, capacity to form biofilm and in vitro adhesion to epithelial cells. All isolates were shown to be resistant to vancomycin and teicoplanin, as well as to two or more other antimicrobials. All isolates harbored at least one putative virulence marker, and the prevalence was as follows: esp, 87.5%; efaA, 82.5%; gelE, 70%;and cylA, 65%. The presence of 4 genes was observed in 32.5% isolates. The presence of the efaA was associated with the presence of esp, regardless of the source of the isolates. A positive association with the presence of cylA and hemolytic activity in the sheep blood agar assay was observed. No association was found for gelE and gelatinase production in the agar plate assay, for efaA and LLC-MK2 cell adhesion, and for esp and biofilm formation on polystyrene surface. These results show the presence of putative virulence genes in multiple antimicrobial resistant E. faecium isolates from different sources in a hospital setting.