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M.Z.A. Rafiquee,Masoom R. Siddiqui,H.N. Haque,M. Shamsul Ola,Hamad A. Al-Lohedan,Z.A. ALOthman,Saikh M. Wabaidur 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-
Reduction of molecular oxygen by 2-mercaptoethanol in the presence of cobalt(II)histidine is described. Cobalt(II)histidine complex forms dinuclear 2:1 (Co:O2) complex with molecular oxygen (m-dioxyte-trakis(histidinato)dicobalt(II)). The molecular oxygen did not directly react with 2-mercaptoethanol, but,reacted in the form of an adduct with cobalt(II)histidine complex. During the course of the reaction themolecular oxygen is reduced to hydroxide ion while the cobalt(II)histidine complex was oxidized tocobalt(III)histidine while 2-mercaptoethanol is reduced into 2,20-dithiodiethanol. The decrease inabsorbance for the dioxygen complex was monitored spectrophotometrically and was observed that thevalues of rate constant increased with the increase in [2-mercaptoethanol]. The values of kobs also increasedwith increasing [NaOH]. Thus, from the results of these studies, mechanism of the reaction has beenproposed. In addition, the values of various equilibrium constants and rate constants were also determinedusing nonlinear least square techniques.
Imam, Hasan,Khan, Mohsin,Gokhale, Nandan S.,McIntyre, Alexa B. R.,Kim, Geon-Woo,Jang, Jae Young,Kim, Seong-Jun,Mason, Christopher E.,Horner, Stacy M.,Siddiqui, Aleem National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.35
<P>N6-methyladenosine (m(6)A) RNA methylation is the most abundant epitranscriptomic modification of eukaryotic messenger RNAs (mRNAs). Previous reports have found m(6)A on both cellular and viral transcripts and defined its role in regulating numerous biological processes, including viral infection. Here, we show that m(6)A and its associated machinery regulate the life cycle of hepatitis B virus (HBV). HBV is a DNA virus that completes its life cycle via an RNA intermediate, termed pregenomic RNA (pgRNA). Silencing of enzymes that catalyze the addition of m(6)A to RNA resulted in increased HBV protein expression, but overall reduced reverse transcription of the pgRNA. We mapped the m(6)A site in the HBV RNA and found that a conserved m(6)A consensus motif situated within the epsilon stem loop structure, is the site for m(6)A modification. The epsilon stem loop is located in the 3' terminus of all HBV mRNAs and at both the 5' and 3' termini of the pgRNA. Mutational analysis of the identified m(6)A site in the 5' epsilon stem loop of pgRNA revealed that m(6)A at this site is required for efficient reverse transcription of pgRNA, while m(6)A methylation of the 3' epsilon stem loop results in destabilization of all HBV transcripts, suggesting that m(6)A has dual regulatory function for HBV RNA. Overall, this study reveals molecular insights into how m(6)A regulates HBV gene expression and reverse transcription, leading to an increased level of understanding of the HBV life cycle.</P>
On a class of modied new Bernstein operators
M. A. Siddiqui,R. R. Agrawal,N. Gupta 장전수학회 2014 Advanced Studies in Contemporary Mathematics Vol.24 No.1
In this paper, by giving a slight modification of the class of new Bernstein type operators introduved in [3], we introduce a class of modified new Bernstein type operators and claim that the midified new Bernstein type operators is more close to classcial Bernstein polynomial in comparison to operators defined in [3]. We prove Voronovskaja type theorem for these operators. We also present a q-analogue of these operators which is an interesting generalizaiton of Bernstein polynomials based on the q-integers, introduved by G M Phillips in [10].
Experimental analysis of electromagnetic vibration damping of a cantilever beam
M. R. Siddiqui,I. Ahmad,M. Khan,S.M. Ahmad 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10
Vibration attenuation is necessary in many electro-mechanical systems. In this paper electromagnetic damping is applied on a flexible cantilever beam (FCB) made of ferromagnetic material. The lateral vibration is controlled by employing two electromagnetic poles mounted in the same axis as that of a vibrating cantilever beam. A power amplifier is developed to provide desired current to the electromagnet. Comparison of damping achieved at different electromagnetic forces is presented. The study employs a new method of applying a constant DC power to one of the electromagnets while other is connected to a variable current source. Experimental results are presented that show the effectiveness of the presented approach.
Study on Factors Leading to Seasonality of Reproduction in Dairy Buffaloes. I. Nutritional Factors
Qureshi, M.S.,Samad, H.A.,Habib, G.,Usmani, R.H.,Siddiqui, M.M. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.7
Fifth one Nili-Ravi dairy buffaloes were studied in North-west Frontier Province of Pakistan. Rectal examination of reproductive organs was carried out on days 14 and 21 and then fortnightly. Milk samples were collected and analyzed for progesterone levels (MPL). Feed samples were collected fortnightly and analyzed. The buffaloes calving during the normal breeding season (NBS, August to January) (p<0.01) postpartum estrus interval of 55.95 days versus 91.15 days in those calving during the low breeding season (LBS, February to July). MPL in the LBS remained lower than the NBS (p<0.01). Shortest postpartum ovulation interval was noted during autumn(August to October), followed by winter (November to January), summer (May to July) and spring (February to April). The incidence of silent ovulations was during LBS than NBS (70.6% versus 29.4%). In autumn there was minimum intake of crude protein (CPI) and maximum intake of metabolizable energy (MEI, p<0.01). Calcium intake was higher in NBS than LBS calving buffaloes (p<0.01). Phosphorus, copper and magnesium intake was lower (p<0.05) and zinc intake was higher (p<0.01) in autumn, It was (p<0.05) and zinc intake was higher (p<0.01) in autumn, It was concluded that onset of breeding season was associated with increasing MEI and decreasing CPI and minerals intake.
Siddiqui, Z. S.,Cho, J. I.,Kwon, T. R.,Ahn, B. O.,Lee, K. S.,Jeong, M. J.,Ryu, T. H.,Lee, S. K.,Park, S. C.,Park, S. H. POLISH ACADEMY SCIENCES WARSAW 2014 ACTA PHYSIOLOGIAE PLANTARUM Vol.36 No.5
The methionine sulfoxide reductase B2 (MsrB2) gene catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine and is known to provide tolerance to biotic and abiotic environmental stresses. There are yet to be any reports that MsrB2 enhances drought tolerance. Two drought-tolerant transgenic rice lines, L-8 (single copy) and L-23 (two copy), expressing the Capsicum annuum MsrB2 (CaMsrB2) gene were selected for stress tolerance phenotyping under drought stress conditions. CaMsrB2 enhanced relative water content, maintained substantial quantum yield (F (v) /F (m) ratio), and subsequently improved photosynthetic pigments. Interestingly, L-23, carrying two-copy T-DNA insertion, showed greater drought tolerance through more effective stomatal regulation, carotenoid concentration, and osmotic potential than the wild type. High-tech infrared technology (FLIR SC620) was used for the selection of stress-tolerant physiotypes. Later, the IR results were correlated with other tested physiological parameters. The IR images, average plant temperature, and physiological parameters of the treated plants are discussed in detail.
Saikh M. Wabaidur,Zeid A. AlOthman,Masoom R. Siddiqui,Kazi Mohsin,Leda G. Bousiakou,George A. Karikas 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-
Ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method has been developed for the simultaneous determination of pharmaceuticals in industrial waste water samples. The binary mobile phase was used a mixture of acetonitrile and 0.1% aqueous solution of formic acid at a flow rate of 0.4 ml min1. Identification and quantitation of the analyzed drug samples were carried out using mass spectrometer equipped with electrospray ionization source operated in negative ionization mode. The linear range of the proposed method was found to be in the range of 18–270 ng ml1 for all drugs with limit of detection in the range of 1.25 to 1.89 ng ml1.
Ryan Jarrah,Sally El Sammak,Chiduziem Onyedimma,Abdul Karim Ghaith,F.M. Moinuddin,Archis R. Bhandarkar,Ahad Siddiqui,Nicolas Madigan,Mohamad Bydon 대한척추신경외과학회 2022 Neurospine Vol.19 No.2
Objective: To comprehensively characterize the utilization of alginate hydrogels as an alternative treatment modality for spinal cord injury (SCI). Methods: An extensive review of the published literature on studies using alginate hydrogels to treat SCI was performed. The review of the literature was performed using electronic databases such as PubMed, EMBASE, and OVID MEDLINE electronic databases. The keywords used were “alginate,” “spinal cord injury,” “biomaterial,” and “hydrogel.” Results: In the literature, we identified a total of 555 rat models that were treated with alginate scaffolds for regenerative biomarkers. Alginate hydrogels were found to be efficient and promising substrates for tissue engineering, drug delivery, neural regeneration, and cellbased therapies for SCI repair. With its ability to act as a pro-regenerative and antidegenerative agent, the alginate hydrogel has the potential to improve clinical outcomes. Conclusion: The emerging developments of alginate hydrogels as treatment modalities may support current and future tissue regenerative strategies for SCI.
Oxidative CO_2 reforming of CH4 over Ni/a-Al_2O_3 catalyst
A.S.A. Al-Fatesh,A.A. Ibrahim,A.H. Fakeeha,A.E. Abasaeed,M.R.H. Siddiqui 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
Oxidative carbon dioxide reforming of methane to synthesis gas over alumina-supported Ni catalysts was investigated at atmospheric pressure. The reforming reactions were carried out using a CO_2 to CH_4feed ratio of one and reaction temperatures in the range 600–800℃The activity and stability of the catalyst, carbon deposition, and synthesis gas (H_2/CO) ratio were determined. Catalyst deactivation was primarily due to coke formation. energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM) and thermo-gravimetric/differential analyzer (TG/DTA) techniques were used to confirm carbon deposition. It was observed that increasing the oxygen feed concentration and/or reaction temperatures,enhanced methane conversion and reduced coke formation. When 20% O_2 feed was used at 800℃,catalyst stability test revealed a 99.7% CH_4 conversion, 1.0% CH_4 conversion drop and 1% carbon formation.