Distribution and Pathogenicity of Fusarium Species Associated with Soybean Root Rot in Northeast China

Yingying Liu,Xuena Wei,Feng Chang,Na Yu,Changhong Guo,Hongsheng Cai 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.6

Fusarium root rot is an increasingly severe problem in soybean cultivation. Although several Fusarium species have been reported to infect soybean roots in Heilongjiang province, their frequency and aggressiveness have not been systematically quantified in the region. This study aimed to investigate the diversity and distribution of Fusarium species that cause soybean root rot in Heilongjiang province over two years. A total of 485 isolates belonging to nine Fusarium species were identified, with F. oxysporum and F. solani being the most prevalent. Pot experiments were conducted to examine the relative aggressiveness of different Fusarium species on soybean roots, revealing that F. oxysporum and F. solani were the most aggressive pathogens, causing the most severe root rot symptoms. The study also assessed the susceptibility of different soybean cultivars to Fusarium root rot caused by F. oxysporum and F. solani. The results indicated that the soybean cultivar DN51 exhibited the most resistance to both pathogens, indicating that it may possess genetic traits that make it less susceptible to Fusarium root rot. These findings provide valuable insights into the diversity and distribution of Fusarium species that cause soybean root rot and could facilitate the development of effective management strategies for this disease.

Oxalate formation during hydrogen peroxide-reinforced oxygen delignification

Yingying Liu,Shujuan Ge,Youming Li,Bingyun Li,Hailong Li 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

The effects of different process parameters (alkali dosage, hydrogen peroxide dosage, temperature, reaction time, and oxygen pressure) on oxalate formation during hydrogen peroxide-reinforced oxygen delignification (OP) of Eucalyptus kraft pulp were investigated in the present study. The relationships between oxalate formation and the dosages of both alkali and peroxide were found to be almost linear. Oxalate formation could be divided into “fast” and “slow” periods, depending on the reaction time. The best selectivity for the OP process was achieved using an alkali dosage of 2.0%, a hydrogen peroxide dosage of 2.0%, a temperature of 100 °C, an oxygen pressure of 0.6 MPa and a reaction time of 60 min. Oxalate formation was also, to some extent, reduced under these conditions. These results will be very helpful in optimizing the OP process, controlling oxalate formation and improving product quality.

Cellulose nanofibrils composite hydrogel with polydopamine@zeolitic imidazolate framework-8 encapsulated in used as efficient vehicles for controlled drug release

Yingying Liu,Ying Huo,Qing Fan,Mei-Ling Liu,Hongbin Liu,Bin Li,Youming Li 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.102 No.-

Herein, we report a nanocellulose hydrogel that has a packaging structure for on-demand drug release. Zeolitic imidazolate frameworks-8 (ZIF-8) were grown on the surface of polydopamine (PDA) to producePDA@ZIF-8 nanoparticles. The obtained PDA@ZIF-8 nanoparticles were then incorporated into a networkof cellulose nanofibrils (CNFs) to fabricate a PDA@ZIF-8/CNFs composite hydrogel. The loading ratio of thedrug tetracycline hydrochloride (TH) was 58%. A slight burst release behavior was observed at the beginning,and the composite hydrogel presented a significant pH-dependent release behavior. The drug deliverytime of the PDA@ZIF-8/CNFs composite hydrogel was 3.5 times longer than that of the PDA/CNFscomposite hydrogel. Furthermore, NIR light radiation accelerated the drug delivery rate. The drug releasemechanism was mainly driven by anomalous transport. Importantly, the novel PDA@ZIF-8/CNFs compositehydrogel was found to be biocompatible according to in vitro cytotoxicity test. Because of its uniquemultiple drug release responses, the PDA@ZIF-8/CNFs composite hydrogel may be used in future clinicalapplications as a promising drug delivery carrier.

Proteomic analyses on the browning of shade-dried Thompson seedless grape

Liu Fengjuan,Huang Wenshu,Feng Zuoshan,Tao Yongxia,Fan Yingying,He Weizhong,Li XiaoLi,Fang Xiaotong,Wang Cheng,Bai Yujia 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.3

China is one of the main producers in the worldwide raisin market. Most China’s raisins are produced in Xinjiang where the Thompson seedless grape ( Vitis vinifera L.cv.Thompson seedless) is the main variety of green raisin. However, the browning of Thompson seedless grape during drying has been well-acknowledged as the primary factor affecting the development of the raisin industry. Data independent acquisition (DIA)-based protein profiling was performed on fresh and shade-dried Thompson seedless grapes. As a result, 5431 proteins were identified, among which the amounts of 739 proteins in fresh grape were found to be significantly different with those in dried grape. The functional annotation based on the Blast2GO showed that the ‘organic substance metabolic process’, ‘regulation of molecular function’, ‘enzyme regulator activity’, and ‘isomerase activity’ related proteins became very active during browning. Further analyses revealed that the browning-related proteins, which with significant different amounts in fresh and in dried grapes, are primarily involved in the phenylpropanoid biosynthesis, tyrosine metabolism, phenylalanine metabolism, oxidative phosphorylation metabolism, plutathione metabolism, peroxisome pathway, and fatty acid degradation. And five random differential proteins were verified with parallel reaction monitoring (PRM). The PRM results were in agreement with the DIA data. The main browning-related proteins of Thompson seedless grape were identified in this study. Their properties were tested, and their roles in the browning mechanism were indicated. This will lay base to a better understanding on the enzymatic browning of Thompson seedless grape, and it will also provide guidance for controlling the quality of Thompson seedless grapes in industry.

Population genetic structure and phylogenetic analysis of gray’s pipefish, Halicampus grayi in the South China Sea

Yingying Wu,Xin Wang,Shuaishuai Liu,Hao Luo,Qiang Lin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background The evolution of male pregnancy is the most distinctive characteristic of syngnathids, and they were recognized as flagship species for marine conservation. Genus Halicampus is an important branch of syngnathid fishes that has not received the attention it deserves. Objective To sequence the mitochondrial genome of Halicampus grayi, and investage the genetic structure of its populations. Methods Degenerate primers were designed to amplify the entire mitochondrial genome of H. grayi. The phylogenetic relationship between H. grayi and other syngnathids were conducted using maximum-likelihood method. Population genetic structure of three geographic population of H. grayi were determined using median-joining haplotype network based on COI and Cytb sequences. Results The complete mitochondrial genome of Halicampus grayi was assembled into a 17,059 bp circular sequence, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 D-loop region. The overall base composition of H. grayi is 29.93% A, 29.31% T, 16.23% G and 24.54% C, with a slight A + T rich feature (59.24%). Phylogenetic analysis indicated that H. grayi has a close relationship with Trachyrhamphus serratus. Population genetic analysis revealed a relatively high genetic diversity across different geographic populations of H. grayi, and the results of median-joining haplotype network indicated a lack of structure in populations of H. grayi. Conclusion The mitogenome of H. grayi will provided important information about the origin and evolution issues of syngnathid fishes, and the high-level genetic diversity detected in their populations will provide insight into the gene flow pattern of marine fishes.

Ferulic acid exhibits anti-inflammatory effects by inducing autophagy and blocking NLRP3 inflammasome activation

Liu Yongjuan,Shi Lu,Qiu Wenhong,Shi Yingying 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4

Background Inflammation is involved in the healing process; however, when inflammation is overactivated, multiple diseases can occur. The continued discovery of new anti-inflammatory drugs is crucial in the treatment of inflammation-linked diseases. Objectives Ferulic acid (FA), a precursor necessary for lignan synthesis, is widely distributed in plant-based whole foods and is a strong antioxidant. However, the effect of FA on the expression level of inflammatory factors in macrophages has not been fully clarified. The current study aimed to explore the anti-inflammatory effect and mechanism of ferulic acid. Results The results showed that THP-1 cells were induced to differentiate into macrophages by Phorbol-12-myristate- 13-acetate (PMA), and THP-1-derived macrophages were stimulated by LPS to establish an inflammatory cell model. Compared with the control group, low (5 μmol·mL −1 ), medium (10 μmol·mL −1 ), and high (20 μmol·mL −1 ) concentration ferulic acid groups have decreased cell viability and increased apoptosis rate in a dose-dependent manner. FA reduced the transcriptional levels of Interleukin-1β (IL-1β), Interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Importantly, FAinduced autophagy and inhibited NLRP3 inflammasome activation. 3-MA (a widely used autophagy inhibitor) enhanced the secretion of TNF-α, IL-6 and IL-1β. Moreover, autophagy inhibition by 3-MA resulted in increased proteins expression associated with NLRP3 inflammasome signaling pathway. Besides, the inhibition of inflammasome activation by MCC950 reduced the expression of TNF-α, IL-6 and IL-1β. Conclusion It is concluded that FA enhanced autophagy, inhibited the activation of NLRP3 inflammasome and reduced the expression and release of inflammatory factors.

Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers

Zhang Yingying,Liu Yingsen,Li Jiaolong,Xing Tong,Jiang Yun,Zhang Lin,Gao Feng 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: This study was conducted to investigate the effects of dietary corn resistant starch (RS) on the intestinal morphology and barrier functions of broilers. Methods: A total of 320 one-day-old broilers were randomly allocated to 5 dietary treatments: one normal corn–soybean (NC) diet, one corn–soybean–based diet supplementation with 20% corn starch (CS), and 3 corn–soybean–based diets supplementation with 4%, 8%, and 12% corn resistant starch (RS) (identified as 4% RS, 8% RS, and 12% RS, respectively). Each group had eight replicates with eight broilers per replicate. After 21 days feeding, one bird with a body weight (BW) close to the average BW of their replicate was selected and slaughtered. The samples of duodenum, jejunum, ileum, caecum digesta, and blood were collected. Results: Birds fed 4% RS, 8% RS and 12% RS diets showed lower feed intake, BW gain, jejunal villus height (VH), duodenal crypt depth (CD), jejunal VH/CD ratio, duodenal goblet cell density as well as mucin1 mRNA expressions compared to the NC group, but showed higher concentrations of cecal acetic acid and butyric acid, percentage of jejunal proliferating cell nuclear antigen-positive cells and delta like canonical Notch ligand 4 (Dll4), and hes family bHLH transcription factor 1 mRNA expressions. However, there were no differences on the plasma diamine oxidase activity and D-lactic acid concentration among all groups. Conclusion: These findings suggested that RS could suppress intestinal morphology and barrier functions by activating Notch pathway and inhibiting the development of goblet cells, resulting in decreased mucins and tight junction mRNA expression.

DHA-enriched phosphatidylcholine suppressed angiogenesis by activating PPARγ and modulating the VEGFR2/Ras/ERK pathway in human umbilical vein endothelial cells

Yuanyuan Liu,Yingying Tian,Yao Guo,Ziyi Yan,Changhu Xue,Jingfeng Wang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.12

Docosahexaenoic acid-enriched phosphatidylcholine(DHA-PC) is a new generation of omega-3 lipids,which contains an ester bond linking DHA at the sn-2position of phospholipid. DHA-PC has become the interestrecently as its better bioavailability and anti-oxidationcapacity. In this study, the anti-angiogenic effect of DHAPCwas evaluated. The capacities of proliferation, migration,tube formation of human umbilical vein endothelialcells were significantly declined after DHA-PC treatment. Furthermore, DHA-PC inhibited the neovascularization ofthe chick chorioallantoic membrane in vivo. Mechanismresults indicated that DHA-PC enhances the expression ofperoxisome proliferator-activated receptor c (PPARc) attranscriptional and translational level, subsequently downregulatesthe VEGFR2 expression and VEGFR2-mediateddownstream Ras/ERK pathway, resulting in significantreduction in proliferation and differentiation. Additionally,PPARc-specific antagonist GW9662 partly reversed theinhibition effects of DHA-PC on tube formation and neovascularization,suggesting that DHA-PC exerts anti-angiogenesiseffect through activating PPARc. Thesefindings indicated that DHA-PC has a great prospect ofanti-tumor angiogenesis therapy.

Deformation path and springback behavior in double-curved bending at high temperature

Po Liu,Taichao Zhang,Bin Guo,Li Yang,Debin Shan,Yingying Zong 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.9

In this work, integrated double-curved bending–sizing–unloading is simulated for a Ti6Al4V titanium alloy sheet. Bending radii R30 mm × R30 mm and R30 mm × R15 mm are used in the bending tests at 700 °C and 750 °C, respectively. A holding time of 0–600 s is applied to explore the effect of sizing time on forming accuracy. Similar experimental tests are performed for comparison with the finite element analysis results. Results show that bending behavior varies remarkably with the bidirectional radii. As for equal bidirectional curvature, bending along each direction occurs simultaneously. Given that bidirectional radii are different, the sheet consecutively experiences single small-, single large-, and double-curved bending. The deformation path results in nonuniform plastic strain distribution. The springback amount increases from the center to the marginal middle zone. Sizing at 700 °C or 750 °C in 600 s or 180–600 s can remarkably reduce the springback amount, respectively. The springback prediction via finite element method is consistent with that of the experiment.

Selection of Potential Virulence Factors Contributing to Streptococcus suis Serotype 2 Penetration into the Blood-Brain Barrier in an In Vitro Co-Culture Model

( Hongtao Liu ),( Seng Zhu ),( Yingying Sun ),( Na Li ),( Jingmin Gu ),( Changjiang Sun ),( Xin Feng ),( Wenyu Han ),( Jianxia Jiang ),( Liancheng Lei ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1

Meningitis caused by Streptococcus suis serotype 2 (S. suis 2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential S. suis 2 virulence factors have been identified. This greatly hinders the progress of S. suis 2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library of S. suis 2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing to S. suis 2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis of S. suis 2 meningitis and potential targets for the development of drug therapies.