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조영숙,박석규,박정로,김종홍,이종일,문주석 순천대학교 기초과학연구소 1993 基礎科學硏究誌 Vol.4 No.-
Tubers of Korean yams were analyzed in proximate, amino acid and fatty acid compositions. The yams were composed of moisture (70.6-86.1%), protein (5.8-9.2%), crude fat (0.2-0.9%) and reducing sugar (0.7-2.9%). The pH and titratable acidity of yam juice were 6.0-6.3 and 0.9-1.1 ㎎%, respectively. Total amino acid contents of the yams were 21.2-26.9% comprising essential amino acids of 49.2-57.3%, and the major amino acids were glutamic acid (2.9-4.3%), arginine (3.0-4.2%) and aspartic acid (2.2-2.8%). Thirteen fatty acids were detected, and the major fatty acids were linoleic (42.2-49.4%), palmitic (25.5-27.8%) and oleic acid (4.7-13.7%).
조수헌,하미나,한상환,주영수,성주헌,강종원,윤덕로,송동빈,이명학,김선태 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2
To determine the recovery time from noise-induced temporary threshold shift(TTS), a prospective field study was conducted at three worksites where workers are known to be exposed high level of noise. Subjects were selected according to answers on a questionnaire which inquired about otological history and previous noise exposure, including avocational, military and occupational exposures. After excluding employee with past otologic problems, recent exposure to high level noise, and under medications, total 92 employees participated in the study. Among 92 participants, complete consecutive audiometric examinations were carried out at 0∼2 hours, 5∼7 hours, 14∼16 hours after worktime noise exposure on 26 participants wearing hearing protectors and 22 participants wearing no protective devices. The difference between the hearing level 0∼2 hours after noise exposure and 5∼7 hours is statistically significant by paired t-test(p<0.01). The median recovery times calculated from the data of 22 participants wearing no protective hearing devices are 15.6 hours at 4000Hz, and 7.7 hours, 10.3 hours, 8.4 hours at 1000Hz, 2000Hz and 8000Hz respectively. These data suggest that when measuring the pure tone audiometry for noise exposed workers, at least 16 hours noise-free interval is required.
Ju Kyong Lee,Jong Wook Chung,Yong Jin Park,Kyung Ho Ma,Hee Kyoung Kang,Jung Ro Lee,Jae Gyun Kwag,Nam Soo Kim,Tae San Kim,Sok Young Lee 한국육종학회 2006 한국육종학회지 Vol.38 No.2
Genetic diversity of Korean landrace rice accessions was assessed with microsatellite markers. The 214 alleles weregedfrom 3 for SSS locus to 37 for RM206 locus with an average number of 12.6 alleles per locus. Gene diversity values according tothe 17 mic
Molecular characterization of an α-N-acetylgalactosaminidase from Clonorchis sinensis.
Lee, Myoung-Ro,Yoo, Won Gi,Kim, Yu-Jung,Kim, Dae-Won,Cho, Shin-Hyeong,Hwang, Kwang Yeon,Ju, Jung-Won,Lee, Won-Ja Springer International 2012 Parasitology research Vol.111 No.5
<P>The α-N-acetylgalactosaminidase (α-NAGAL) is an exoglycosidase that selectively cleaves terminal α-linked N-acetylgalactosamines from a variety of sugar chains. A complementary DNA (cDNA) clone encoding a novel Clonorchis sinensis α-NAGAL (Cs-α-NAGAL) was identified in the expressed sequence tags database of the adult C. sinensis liver fluke. The complete coding sequence was 1,308 bp long and encoded a 436-residue protein. The selected glycosidase was manually curated as α-NAGAL (EC 3.2.1.49) based on a composite bioinformatics analysis including a search for orthologues, comparative structure modeling, and the generation of a phylogenetic tree. One orthologue of Cs-α-NAGAL was the Rattus norvegicus α-NAGAL (accession number: NP_001012120) that does not exist in C. sinensis. Cs-α-NAGAL belongs to the GH27 family and the GH-D clan. A phylogenetic analysis revealed that the GH27 family of Cs-α-NAGAL was distinct from GH31 and GH36 within the GH-D clan. The putative 3D structure of Cs-α-NAGAL was built using SWISS-MODEL with a Gallus gallus α-NAGAL template (PDB code 1ktb chain A); this model demonstrated the superimposition of a TIM barrel fold (α/β) structure and substrate binding pocket. Cs-α-NAGAL transcripts were detected in the adult worm and egg cDNA libraries of C. sinensis but not in the metacercaria. Recombinant Cs-α-NAGAL (rCs-α-NAGAL) was expressed in Escherichia coli, and the purified rCs-α-NAGAL was recognized specifically by the C. sinensis-infected human sera. This is the first report of an α-NAGAL protein in the Trematode class, suggesting that it is a potential diagnostic or vaccine candidate with strong antigenicity.</P>
Skin seeding technique using 0.5-mm micro-punch grafting for vitiligo: An animal and clinical study
( Ro Woo Lee ),( Hyun Jeong Ju ),( Gyong Moon Kim ),( Han Na Lee ),( Sung Hye Eun ),( Soo Hyung Kim ),( Jiehoon Kim ),( Dong Seok Kim ),( Jung Min Bae ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2
Background: Among various surgical interventions for vitiligo, punch grafting is a simple and convenient way to treat refractory vitiligo. Objectives: To examine the efficacy and safety of the 0.5-mm micro-punch graft in treatment of stable vitiligo and to compare in terms of orientation of punch graft transplanted. Methods: A total of 100 lesions of 50 patients with stable vitiligo were included in this comparative study. Paired recipient patches from each patient were treated by motorized 0.5-mm micro-punch graft and donor punch grafts were implanted in a chamber in opposite direction, respectively; one lesion was transplanted in epidermal orientation and the other in dermal orientation. The extent of repigmentation was assessed by 4-point scale (poor, fair, good, and excellent). Results: Treatment success (≥75% repigmentation) was achieved in 72% of epidermal orientation group and 76% of dermal orientation group. Cobblestone appearance was observed in 26% of epidermal orientation group and 24% of dermal orientation group. Conclusion: Motorized 0.5-mm micro-punch grafting is a simple and effective treatment option in refractory, stable vitiligo. Practitioner can plant the graft without considering the orientation and we named this technique “Skin seeding technique”.
Design of ICA to Extract Respiration Signal From PPG Signal
Lee, Ju-Won,Lee, Byeong-Ro The Korea Institute of Information and Commucation 2011 Journal of information and communication convergen Vol.9 No.2
Respiration signal of the vital signs is an important parameter in clinical parts. To extract the respiration signal from PPG signal for mobile healthcare system is difficult because the bands of the motion artifacts and respiration in the frequency domain are overlapped. This study to improve this problem suggested a respiration extraction method using the independent component analysis and evaluated its performances. In results of evaluation, the ICA method showed better performance than LPF suggested recently.
Lee, Seoghyun,Ro, Hyunju,In, Hyun Ju,Choi, Ji-Hee,Kim, Mun-Ock,Lee, Jinhyuk,Hong, Sung-Tae,Lee, Su Ui Elsevier 2018 Cytokine Vol.108 No.-
<P><B>Abstract</B></P> <P>Fisetin (3,7,3′,4′-tetrahydroxyflavone), a natural flavonoid, is a therapeutic agent for respiratory inflammatory diseases such as chronic obstructive pulmonary disease (COPD). However, detailed molecular mechanisms regarding the target protein of fisetin remain unknown.</P> <P>Fisetin significantly reduces tumour necrosis factor alpha (TNF-α)-induced interleukin (IL)-8 levels by inhibiting both nuclear factor kappa B (NF-κB) transcriptional activity and the phosphorylation of its upstream effectors. We show that fisetin prevents interactions between protein kinase C (PKC)δ and TNF receptor-associated factor 2 (TRAF2), thereby inhibiting the inhibitor of kappa B kinase (IKK)/NF-κB downstream signalling cascade. Furthermore, we found that fisetin directly binds to PKCδ <I>in vitro</I>. Our findings provide evidence that fisetin inhibits the TNF-α-activated IKK/NF-κB cascade by targeting PKCδ, thereby mediating inflammatory diseases such as COPD.</P> <P>These data suggest that fisetin is a good therapeutic drug for the treatment of inflammatory lung diseases, such as COPD, by inhibiting the TNF-α/NF-κB signalling pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fisetin inhibits TNF-α-increased <I>IL-8</I> gene expression by blocking NF-κB activity. </LI> <LI> Fisetin inhibits TNF-α-mediated interactions between PKCδ and TRAF2. </LI> <LI> Fisetin inhibits PKCδ activity. </LI> </UL> </P>
Lee, Kyung Ah,Yoon, Ki Ro,Kwon, Sung Hyun,Lee, Kyung Jin,Jo, Sunhee,Lee, Ju Sung,Lee, Kwan-Young,Lee, Seung Woo,Lee, Seung Geol,Kim, Jin Young The Royal Society of Chemistry 2019 Journal of materials chemistry. A, Materials for e Vol.7 No.13
<P>Self-assembly of inter- and intraphases between the ionomer membrane and mechanical reinforcements in reinforced polymeric composite membranes is of great interest in both physics and chemistry, particularly for proton exchange membrane fuel cell (PEMFC) application. One of the greatest challenges is to obtain an optimally structured polytetrafluoroethylene (PTFE)-reinforced perfluorosulfonic acid (PFSA) composite membrane <I>via</I> solution processing. For high- performance and durable PEMFC applications, the self-assembly of interphases of the ionomers/reinforcements and intraphases of the ionomers in the composite PEM needs to be carefully tailored. Here, we show that post-assembly modification of a PTFE/PFSA composite membrane using a spin-drying method can significantly improve fuel cell performance and reliability. The forced assembly of PFSA ionomers during spinning results in greater infiltration yields into the porous PTFE media. In this way, spin-drying post-treatment results in lower gas crossover and improved mechanical durability. This process also yields larger regions of ionic clusters, which facilitate proton conduction at low relative humidity, affording higher fuel cell currents and greater efficiency.</P>