일부 한국인의 혈청 ceruloplasmin 정상 참고치 설정 및 질환별 비교 : Comparison of Its Level among Various Diseases

이동수,박애자,박정덕,장임원,홍연표 중앙대학교 의과대학 의과학연구소 1999 中央醫大誌 Vol.24 No.1

Ceruloplasmin has been investigated as one of the useful biologic index for early diagnosis of atherolsclerosis. However, there was no normal reference value taking into account the confounders in Korea. This study was performed to estimate the normal reference values and present the confounders of ceruloplasmin. Also we compared of serum ceruloplasmin levels among normal reference group and three abnormal groups: 475 healthy subjects, 53 liver dysfunctions, 72 positive test of chronic inflammatory diseases, and 89 hypercholesterolemias. Mean serum ceruloplasmin level of healthy subjects were 41.6±7.6㎎/㎗ in males and 42.1±7.2㎎/㎗ in females, respectively. It was tend to increase of ceruloplasmin by age in both sexes. However, the statistical difference was not found by age group between both sexes. Statistically significant correlations were found between serum ceruloplasmin and age, body mass index(BMI), white blood cell(WBC) and triglyceride(TG) in males and age, BMI, WBC and total cholestrol(TG) in females. After adjustment for age, BMI,WBC and TG in males and age, BMI, WBC and TG in females, significant elevations of serum ceruloplasmin were found in liver dysfunction(p=0.0077) and positive test of chonic inflammatory disease(p=0.0217) in males and in oisitive test of chronic inflammatory disease(p=0.0023) and hypercholestolemia(p=0.0124) in females relative to healthy reference subjects. There is no statistical difference among liver dysfunction, postive test of chronic inflammatory disease, and hypercholesterolemia.

계면활성제 혼합 천연지방산유지 고형 비누의 세척성 및 생분해성 연구 : 생분해성을 중심으로 On Based Biodegradation

이봉연,류덕환,이태관 한국의류산업학회 2003 한국의류산업학회지 Vol.5 No.3

The purpose of the study was to get the valuable data for developing the new natural fat soaps which have an excellent biodegradation performance. Thus, natural fat soaps mixed with the two types of detergents (AOS and LAS) on the various concentrations were made and the biodegradation of the samples were analysed by Dissolved Oxygen method using active sludge. Also, the results were compared with the commercial synthetic detergents and market soaps. The results from the study were the followings: I. The plant fat soap and the wasted oil soap with the concentration of 5 mg/i and 15 mg/1 had an excellent biodegradation rather than animal fat soap. 2, There was little difference among samples with the concentration of 5 mgtl, but there was much difference among them with the concentration of 15 mg/l. 3. The periods for consuming oxygen of wasted oil soap mixed AOS and LAS was the fastest.

카드뮴폭로후 고환, 신장 및 간장내 카드뮴축적량과 조직손상의 변화

이영구,박정덕,최병선,홍연표,장임원 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.1

To assess the changes of cadmium deposit and histopathologic finding of testis, liver and kidney, different dose of cadmium (Cd) was administerd into male Sprague-Dawley rat(purchased from KIST, Korea Institute of Science and technology) by single intravenous injection. At 12, 24, 48, 168 and 672 hours after Cd injection, tissue cadmium concentration and histopathological change were examined. Cd exposed group showed lesser weight gain than the control. Renal Cd content tended to increase in time-dependent pattern, while hepatic and testicular Cd content tended to decrease. Mean of renal Cd content to hepatic Cd content (Cd K/Cd L) also progressively increased. These results suggest that Cd might be gradually transported to kidney from other organs. In testis and liver, degenerative changes appeared relatively in acute phase. These changes became more pronounced in testis, while less prominent in liver. In kidney, swelling of proximal tubluar cells and thickening of glomerular basement membrane appeared relatively later and these changes were progressively aggravated.

카드뮴폭로에 대한 생물학적 지표 : Competitive ELISA에 의한 Metallothionein 정량 Assay of Metallothionein by Competitive ELiSA

박정덕,이우석,공윤,홍연표,장임원 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

Metallothionein (MT) is a cadmium binding protein that played major roles in protective mechanism for cadmium toxicity. In the present study, competitive ELISA was established to assay the MT expression utilizing monospecific antibody which was generated against MT-I. A total of 10 Sprague-Dawley rats was injected with CdCl₂ for two weeks to induce MT. The cytosolic fraction of rat liver was obtained by differential centrifugation. Two major MT isozymes (MT-I & MT-II) at ca. 10 kDa were purified by Sephadex G-75 gel filtration followed by DEAE-Trisacryl-M anion exchange column chromatography, respectively. Two rabbits were immunized 4 times consecutively with the conjugate of purified MT-I. The sera were collected by heart puncture. When immunoblot was carried out, the immunized rabbit sera (anti-MT-I) exhibited specific immunoreactive band at MT-I while showed any cross reactions neither with MT-II nor with other cytosolic proteins. By chequerboard titration using the monospecific antibody, the competitive ELISA was established. The dose-dependent relationship was observed between anti-MT-I anti-body and the amount of MT in biological samples (r²=0.9980). These results suggested strongly that competitive ELISA is a simple, rapid and reproducible method for screening cadmium exposure.

한국인의 심근 조직내 카드뮴 농도의 참고치

박정덕,임헌방,최병선,권일훈,이상연,강은용,홍연표,장임원 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

Cadmium(Cd) is an ubiquitous, toxic and nonessential metal which is controversial about the association with a cardiovascular disease. In this study, we investigated the reference level of Cd in myocardium in Korean general population. The level of Cd and zinc(Zn) concentration in myocardium of 252 cases of "sudden and unexpected death" autopsies (male: 172, female : 60) aged 0 to 87 years was analyzed. The concentration of Cd and Zn was determined with atomic absorption spectrophotometer(Perkin-Elmer Model 5100) by flameless and flame method, respectively. The content of Cd and Zn in myocardium showed the log-normal distribution rather than normal distribution. Geometric mean concentration of Cd was 0.19 ㎍/g wet weight in myocardium. The level of Cd in myocardium was not significantly different between male (0.18㎍/g wet weight) and female (0.22㎍/g wet weight). Geometric mean concentration of Zn in myocardium was 25.25 ㎍/g wet weight. The level of Zn between male (25.13 ㎍/g wet weight) and female (25.57㎍/g wet weight) was not different. The deposit of Cd in myocardium was age-dependent of biphasic pattern, but the Zn level by age was not significantly different. The level of Cd in myocardium was increased to the fifties of age, thereafter a leveling-off was shown. The regression model of Cd deposit in myocardium by age was predicted as the following equation: Log Heart-Cd= -1.2726 + 0.0234 ·Age -0.0002 ·Age². The maximum Cd deposit in myocardium was estimated to be 0.26 ㎍/g wet weight at the age of 58.5 in Korean general population. In addition, the total Cd burden in heart by age was predicted as a following equation: Total Cd burden in Heart= -10.165+2.891 ·Age -0.0258 ·Age². The maximum heart burden of Cd was estimated to be 70.7㎍ at age of 55.4. The positive correlation between Cd and Zn was observed in myocardium. The linear regression equation was Log Heart-Zn = 1.4195+0.0262 ·Log Heart-Cd.

정신장애인의 재활의 위한 사회기술훈련의 효과성에 관한 연구

황태연,손덕순,백형의,박종원,김지현,이경화 용인정신의학연구소 1999 용인정신의학보 Vol.6 No.2

정신분열증 음성증상에 대한 단기 토큰경제의 효과와 치료인자

안정숙,이연덕,방형석,박정은,신정호 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.5

연구목적 : 정신분열증의 음성증상은 약물치료에 잘 반응하지 않은 채로 남아 재활을 어렵게 한다. 본 연구는 정신분열증환자의 음성증상을 개선시키는데 토큰경제치료(TET)가 효과가 있는 지를 조사하고, 종합병원의 정신과병동 여건에 맞는 TET 프로그램을 개발하고자 시행되었다. 연구방법 : 정신과병동에 입원치료중 음성증상이 주문제가 되어 삼개월 이하의 단기 TET를 받은 정신분열증환자 35사례를 대상으로, PANSS음성증상척도를 사용하여 TET의 효과를 조사하였다. 또한 목표행동의 각 수준별로 성공집단과 실패집단의 환자 변인과 프로그램 변인을 비교함으로써 치료인자를 규명하고자 하였다. 연구결과 : 1) 음성소척도 총점은 기저선 관찰기간의 31.57±4.32점에서 TET 종료시 20.66±4.63점으로 낮아졌다. TET종료시 음성소척도의 7개 증상 모두 감소하였으나 추상적 사고의 장애와 상동적 사고는 다른 5개 소척도 증상에 비해 상대적으로 덜 개선되었다. 또한 TET 기간 동안 항정신증약물의 용량은 487.58±372.79mg/day로부터 291.21±287.74mg/day로 감소하였다. 2) 자조기술 수준(Ⅰ)에 속한 항목들의 성공률은 75%, 병동활동 수준(Ⅱ)에서는 78%, 사회기술 및 작업 수준(Ⅲ)에서는 68%로서, 전체 목표행동에 대한 TET의 성공률은 74%였다. 3) 수준 Ⅱ의 성공집단은 실패집단보다 TET 종료시 항정신병약물의 투약용량이 유의하게 낮았으며, 수준 Ⅲ의 성공집단은 실패집단보다 입원경력이 유의하게 적었다(p<.05). 수준 Ⅰ에서는 간식신청, 면회, 가족외출 등이, 수준 Ⅱ에서는 면회가 강화물로 적용되었을 때 성공률이 유의하게 높았다. 결 론 : 이러한 결과는 TET가 정신분열증환자의 음성증상에 효과적인 치료법임을 보여준다. TET의 효율향상과 원할한 운용을 위해서는 개별화된 프로그램을 개발하려는 지속적인 연구가 필요할 것이다. Objective : The negative symptoms of schizophrenia usually are not responsive to drug therapy to persist and make schizophrenics more difficult in their rehabilitation. The authors investigated the therapeutic effect of token economy(TET) for negative symptoms of schizophrenic patients, and attempted to develop a TET program for psychiatric wards in general hospital. Methods : Negative subscale of PANSS was rated for 35 schizophrenics with negative symptoms as their main problems before and after short-term TET(less than 3 months). Therapeutic factors were identified by comparing patient and program variables between the achieved and the non-achieved group of each level of target behaviors. Results : 1) Subtotal of negative subscale of PANSS was decreased from 31.57±4.32 at baseline to 20.66±4.63 after treatment. With TET, symptoms of 'difficulty in abstract thinking' and 'stereotypic thinking' were less responsive than the other 5 items of the scale. Also the dose of antipsychotics prescribed was reduced to 291.21±287.74mg/day from 487.58±372.79mg/day. 2) The achievement rate of level Ⅰ(self-help) was 75%, level Ⅱ(ward-activity) 78%, and level Ⅲ(social activity/occupation) 68%, resulting in average 74% for all target behaviors. 3) On level Ⅱ, dose of antipsychotics was lower, and on level Ⅲ, number of admission was fewer and female sex was more frequent in achieving patient group than nonachieving group. The rate of achievement was higher in case of special food, reception, and outing with family applied as a reinforcer on level Ⅰ, and reception on level Ⅱ. Conclusion : These results showed that TET was effective for schizophrenic patients with negative symptoms in psychiatric ward. Further investigations to refine the individual program of TET will be needed for enhancement of it's therapeutic efficacy and smart application of it.

말티즈견에서 발생한 승모판폐쇄부전 1례

전소분,박혜연,김선아,정금선,이영원,박성준,김덕환,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 10 years old, intact male, Maltese dog with clinical signs of labored respiration, exercise intolerance and increasing of coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Physical examination revealed tachycardia, panting and mild cyanosis. This case was diagnosed as mitral valve insufficiency by clinical signs, ascultation, blood chemistry, radiography and electrocardiography. This dog was treated with ACE inhibitor (enalapril), diuretics (furosemide) and bronchodilator (aminophylline) for 14 days. After treatment, clnical signs were disappeared and also this dog was needed to be continuous monitoring and sodium restricted diet.

최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

Decreased entropy of unfolding increases the temperature of maximum stability: Thermodynamic stability of a thioredoxin from the hyperthermophilic archaeon Methanococcus jannaschii

Lee, Duck-Yeon,Kim, Kyeong-Ae,Kim, Key-Sun Korean Magnetic Resonance Society 2004 Journal of the Korean Magnetic Resonance Society Vol.8 No.1

A thioredoxin from hyperthermophile, Methanococcus jannashii (MjTRX) was characterized by use of the differential scanning calorimetry to understand the mechanisms of thermodynamic stability. MjTRX has an unfolding transition temperature of 116.5$^{\circ}C$, although the maximum free energy of the unfolding (9.9 Kcal/mol) is similar to that of E. coli thioredoxin (ETRX, 9.0 Kcal/mol). However, the temperature of maximum stability is higher than ETRX by 20$^{\circ}C$, indicating that the unfolding transition temperature increased by shifting the temperature of maximum stability. MjTRX has lower enthalpy and entropy of the unfolding compared to ETRX maintaining a similar free energy of the unfolding. From the structure and the thermodynamic parameters of MjTRX, we showed that the unfolding transition temperature of MjTRX is increased due to the decreased entropy of the unfolding. Decreasing the unfolded state entropy and increasing the folded state entropy can decrease the entropy of the unfolding. In the case of MjTRX, the increased number of proline residues decreased the unfolded state entropy and the increased enthalpy in the folded state increased the folded state entropy.