High dose of QX-314 produces anti-nociceptive effect without capsaicin in rats with inflammatory TMJ pain

Kui-Ye Yang,Min-Su Kim,Eun-Kyung Kim,Mi-Sun Kong,Jong-Soo Ahn,Jong-Hun Lee,Jin-Sook Ju,Dong-Kuk Ahn KOREAN ACADAMY OF ORAL BIOLOGY 2013 International Journal of Oral Biology Vol.38 No.4

The present study investigated the effects of QX-314 on inflammatory pain of the temporomandibular joint (TMJ). Experiments were carried out on male Sprague-Dawley rats weighing 220-280 g. Under anesthesia, the TMJ of each animal was injected with 50 μL of formalin (5%). The number of noxious behavioral responses, including rubbing or scratching of the facial region including the TMJ area, was recorded over 9 sequential 5 min intervals for each animal. Although 2.5% QX-314 did not affect formalin-induced nociceptive behavior, administration of 5% QX-314 with formalin significantly decreased the number of scratches produced by the formalin injection. Co-administration of capsaicin, a TRPV1 agonist, with 2.5% QX-314 produced significant anti-nociceptive effects whereas 2.5% QX-314 alone did not. However, the co-administration of capsaicin did not enhance the anti-nociceptive effects in the 5% QX-314-treated rats. Moreover, the co-administration of capsazepine, a TRPV1 antagonist, did not attenuate anti-nociceptive effects in the 5% QX-314-treated rats. These findings suggest that TRPV1 is effective in the transport of low but not high doses of QX-314. Moreover, a high dose of QX-314, which is not mediated by peripheral TRPV1 activity, may be viable therapeutic strategy for inflammatory pain in the TMJ.

The role of sharing infrastructure for utilization of laboratory animal-derived resources in Korea

Kui Ye Yang(양귀예) 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7

Participation of Central P2X7 Receptors in CFA-induced Inflammatory Pain in the Orofacial Area of Rats

Kui-Ye Yang,Myung-Dong Kim,Jin-Sook Ju,Min-Ji Kim,Dong-Kuk Ahn KOREAN ACADAMY OF ORAL BIOLOGY 2014 International Journal of Oral Biology Vol.39 No.1

We investigated the role of central P2X receptors in inflammatory pain transmission in the orofacial area in rats. Experiments were carried out using male Sprague-Dawley rats weighing 230-280g. Complete Freund's adjuvant (CFA, 40 μL) was applied subcutaneously to the vibrissa pad to produce inflammatory pain. The intracisternal administration of iso-PPADS tetrasodium salt, a non-selective P2X receptor antagonist, A317491 sodium salt hydrate, a P2X2/3 receptor antagonist, 5-BDBD, a P2X4 receptor antagonist, or A438079 hydrochloride, a P2X7 receptor antagonist, was performed 5 days after CFA injection. Subcutaneous injections of CFA produced increases in thermal hypersensitivity. Intracisternal injections of iso-PPADS (25 μg) or A438079 (25 or 50 μg) produced significant anti-hyperalgesic effects against thermal stimuli compared to the vehicle group. A317491 or 5-BDBD did not affect the head withdrawal latency times in rats showing an inflammatory response. Subcutaneous injections of CFA resulted in the up-regulation of OX-42, a microglia marker, and GFAP, an astrocyte marker, in the medullary dorsal horn. The intracisternal administration of A438079 reduced the numbers of activated microglia and astrocytes in the medullary dorsal horn. These results suggest that a blockade of the central P2X7 receptor produces antinociceptive effects, mediated by inhibition of glial cell function in the medullary dorsal horn. These data also indicate that central P2X7 receptors are potential targets for future therapeutic approaches to inflammatory pain in the orofacial area.

고등학생의 구강건강 관련 삶의 질이 학교생활 만족도에 미치는 영향

양귀예 ( Kui-ye Yang ),장지언 ( Ji-eon Jang ) 한국치위생과학회 2016 치위생과학회지 Vol.16 No.2

이번 연구는 고등학생의 구강건강 관련 삶의 질을 측정하여 학교생활 만족도와 상관관계를 알아보고, 청소년의 구강건강 관련 삶의 질이 학교생활 만족도에 미치는 영향을 분석하기 위해 실시하였다. 2015년 11월 30일부터 12월 7일까지 대구에 위치한 1개 고등학교 학생을 대상으로 편의표본추출법에 의한 자기기입 방식으로 설문조사를 실시하여 다음과 같은 결과를 얻었다. 고등학생의 구강건강 관련 삶의 질 중 기능적 삶이 3.51로 가장 높았으며, 학교생활 만족도 중에서는 대인관계 만족도가 3.75로 가장 높았다. 그리고 구강건강 관련 삶의 질에 유의한 영향을 주는 일반적 특성은 학업성적, 학업 스트레스(p<0.05)였으며, 학교생활 만족도에 유의한 영향을 주는 일반적 특성은 학업성적(p<0.001)이었다. 구강건강 관련 삶의 질은 학교생활 만족도의 하위요인 중 대인관계, 교육환경, 사회적 지지와 정(+)의 상관관계를 보였으며, 학교생활 만족도에 영향을 주는 요인으로는 학업성적과 구강건강 안녕 삶의 질이 유의한 영향을 나타내었다. 이상의 연구결과를 종합해보면, 고등학생의 구강건강 관련 삶의 질은 청소년의 학교생활 만족도와 관련되는 다양한 요인에 영향을 미치고 있다는 것을 알 수 있다. 따라서 청소년들이 올바른 구강건강 관리습관을 형성할 수 있도록 정기적인 구강보건교육 프로그램을 개발하여 효과적으로 실행할 수 있는 제도를 마련해야 하며, 학생 및 학교, 그리고 관련 부서에서 구강건강의 중요성을 인식하고 예방 및 조기치료, 구강건강 증진을 위해 노력해야 한다. The purpose of this study was to evaluate the association between oral health-related quality of life and school life satisfaction in high school students. A questionnaire-based survey was conducted on high school students in Daegu, South Korea from November to December, 2015, and final data from 432 students was analyzed. Analysis of oral health-related quality of life in terms of general characteristics showed that both academic achievement and stress were significant factors (p<0.05). With respect to school life satisfaction, academic achievement was found to be a highly significant influencing factor (p<0.01). Correlation analyses of oral health-related quality of life with various factors of school life satisfaction showed positive correlations with personal relationships, educational learning environment, social support. Regression analysis of school life satisfaction showed that academic achievement and oral health-related quality of life were influencing factors. These results indicate that oral health-related quality of life may play a significant role in school life satisfaction.

남자 고등학생의 구강건강관련 자기효능감이 구강건강관련 삶의 질에 미치는 영향

양귀예 ( Kui-ye Yang ),장지언 ( Ji-eon Jang ) 대한예방치과·구강보건학회 2020 大韓口腔保健學會誌 Vol.44 No.1

Objectives: To evaluate the association between oral health-related quality of life and oral healthrelated self-efficacy in high school students. Methods: A questionnaire-based survey was conducted among high school students in Daegu, South Korea from November to December 2015, and the final data of 432 students were analyzed. Results: Analysis of oral health-related self-efficacy in terms of general characteristics showed that both grade and income were significant factors (P<0.01). Furthermore, analysis of oral health-related quality of life in terms of general characteristics showed that academic achievement, father’s educational level, and academic stress were significant factors (P<0.05). Correlation analyses of oral health-related quality of life with oral health-related self-efficacy revealed positive correlations with tooth brushing and ordinary oral health behavior. Regression analysis of oral health-related quality of life showed that father’s educational level, oral health-related self-efficacy, academic stress, and academic achievement were influencing factors. Conclusions: These results indicate that oral health-related self-efficacy may play a significant role in oral health-related quality of life.

Purification and Cloning of an Extracellular Serine Protease from the Nematode-Trapping Fungus Monacrosporium cystosporium

( Jin Kui Yang ),( Feng Ping Ye ),( Qi Li Mi ),( Song Qing Tang ),( Juan Li ),( Ke Qin Zhang ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.5

일부 치과진료 환자의 구강건강상태와 라이프케어를 위한 구강관리용품 인지 및 사용의 관련성

장지언(Ji-Eon Jang),양귀예(Kui-Ye Yang),정선락(Sun-Rak Jeong),최연희(Youn-Hee Choi) 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회논문지 Vol.12 No.2

이 연구는 치과내원 환자의 진료특성에 따른 주관적 구강건강상태, 구강관리용품에 대한 인지 및 사용, 주관적 구강건강상태와 구강관리용품에 대한 인지 및 사용의 관련성을 조사하였다. 자료는 2013년 12월 2일에서 12월 31일까지 약 한 달 동안 대구시 일부 치과의원에 내원하는 592명 환자를 대상으로 자기기입식 설문지조사를 하였다. 환자특성별에 따른 주관적 구강건강상태는 정기구강검진환자가 가장 높았으며, 구강관리용품 인지와 사용은 교정환자가 가장 높았다. 주관적 구강건강상태와 구강관리용품 인지 및 사용의 관련성은 주관적 구강건강상태가 높을 수록 구강관리용품에 대한 인지는 높게 나타났으나 사용은 유의한 관련성이 없는 것으로 나타났으며, 구강관리용품에 대한 인지가 높을수록 구강관리용품 사용이 높게 나타났다. 따라서 주관적 구강건강상태가 높은 환자는 구강관리용품 사용의 필요성을 못 느끼고 있는 반면, 치과를 자주 방문하거나 치과의료진으로부터 적극적인 구강관리용품 교육을 받은 교정환자나 임플란트 환자는 구강관리용품에 대한 인지나 사용이 높았다. 또한 구강관리용품에 대한 인지가 높은 환자일수록 사용이 높기 때문에 구강건강상태가 건강한 환자를 비롯하여 건강하지 못한 환자까지 치과를 방문하는 모든 환자에게 구강상태에 알맞은 구강관리용품의 홍보와 교육이 이루어져야 한다. The purpose of this study was to investigate subjective oral health status according to patient characteristics, Awareness and utilization of oral hygiene devices according to patient characteristics, correlations among subjective oral health status, awareness and utilization of oral hygiene devices. The data were collected from 592 patients who were visited in dental clinic in Daegu from December 2 to December 31, 2013. The subjective oral health status according to the patient characteristics was the highest in the regular oral examination patients, and the orthodontic patients had the highest Awareness and utilization of oral hygiene devices according to patient characteristics. Subjective oral health status was significantly related to the awareness of oral hygiene devices, but not to the utilization of oral hygiene devices. The higher the awareness of oral hygiene devices were, the higher the utilization of oral hygiene devices were. Therefore, patients with high subjective oral health status do not feel the necessity of using oral hygiene devices, and patients who have high awareness of oral hygiene devices are more likely to use them. so, It is necessary that the promotion and education about oral hygiene devices appropriate to their oral health status are made to all patients visiting the dental clinic, including those with healthy oral health patients and unhealthy.

Participation of central GABA_A receptors in the trigeminal processing of mechanical allodynia in rats

Kim, Min Ji,Park, Young Hong,Yang, Kui Ye,Ju, Jin Sook,Bae, Yong Chul,Han, Seong Kyu,Ahn, Dong Kuk The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1

Here we investigated the central processing mechanisms of mechanical allodynia and found a direct excitatory link with low-threshold input to nociceptive neurons. Experiments were performed on male Sprague-Dawley rats weighing 230-280 g. Subcutaneous injection of interleukin 1 beta ($IL-1{\beta}$) ($1ng/10{\mu}L$) was used to produce mechanical allodynia and thermal hyperalgesia. Intracisternal administration of bicuculline, a gamma aminobutyric acid A ($GABA_A$) receptor antagonist, produced mechanical allodynia in the orofacial area under normal conditions. However, intracisternal administration of bicuculline (50 ng) produced a paradoxical anti-allodynic effect under inflammatory pain conditions. Pretreatment with resiniferatoxin (RTX), which depletes capsaicin receptor protein in primary afferent fibers, did not alter the paradoxical anti-allodynic effects produced by the intracisternal injection of bicuculline. Intracisternal injection of bumetanide, an Na-K-Cl cotransporter (NKCC 1) inhibitor, reversed the $IL-1{\beta}$-induced mechanical allodynia. In the control group, application of GABA ($100{\mu}M$) or muscimol ($3{\mu}M$) led to membrane hyperpolarization in gramicidin perforated current clamp mode. However, in some neurons, application of GABA or muscimol led to membrane depolarization in the $IL-1{\beta}$-treated rats. These results suggest that some large myelinated $A{\beta}$ fibers gain access to the nociceptive system and elicit pain sensation via $GABA_A$ receptors under inflammatory pain conditions.

Participation of central GABA_A receptors in the trigeminal processing of mechanical allodynia in rats

Min Ji Kim,Young Hong Park,Kui Ye Yang,Jin Sook Ju,Yong Chul Bae,Seong Kyu Han,Dong Kuk Ahn 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1

Here we investigated the central processing mechanisms of mechanical allodynia and found a direct excitatory link with low-threshold input to nociceptive neurons. Experiments were performed on male Sprague-Dawley rats weighing 230-280 g. Subcutaneous injection of interleukin 1 beta (IL-1β) (1 ng/10 μL) was used to produce mechanical allodynia and thermal hyperalgesia. Intracisternal administration of bicuculline, a gamma aminobutyric acid A (GABA_A) receptor antagonist, produced mechanical allodynia in the orofacial area under normal conditions. However, intracisternal administration of bicuculline (50 ng) produced a paradoxical anti-allodynic effect under inflammatory pain conditions. Pretreatment with resiniferatoxin (RTX), which depletes capsaicin receptor protein in primary afferent fibers, did not alter the paradoxical anti-allodynic effects produced by the intracisternal injection of bicuculline. Intracisternal injection of bumetanide, an Na-K-Cl cotransporter (NKCC 1) inhibitor, reversed the IL-1β-induced mechanical allodynia. In the control group, application of GABA (100 μM) or muscimol (3 μM) led to membrane hyperpolarization in gramicidin perforated current clamp mode. However, in some neurons, application of GABA or muscimol led to membrane depolarization in the IL-1β-treated rats. These results suggest that some large myelinated Aβ fibers gain access to the nociceptive system and elicit pain sensation via GABA_A receptors under inflammatory pain conditions.

Botulinum Toxin Type A Attenuates Activation of Glial Cells in Rat Medullary Dorsal Horn with CFA-induced Inflammatory Pain

Min-Ji Kim,Jin-Ho Cho,Hye-Jin Kim,Kui-Ye Yang,Jin-Sook Ju,Min-Kyung Lee,Min-Kyoung Park,Dong-Kuk Ahn 대한구강생물학회 2015 International Journal of Oral Biology Vol.40 No.2

The activation of glial cells in the spinal cord has been contribute to the initiation and maintenance of pain facilitation induced by peripheral inflammation and nerve injury. The present study investigated effects of botulinum toxin type A (BoNT-A), injected subcutaneously or intracisternally, on the expression of microglia and astrocytes in rats. Complete Freund’s Adjuvant (CFA)-induced inflammation was employed as an orofacial chronic inflammatory pain model. A subcutaneous injection of 40 μL CFA into the vibrissa pad was performed under 3% isoflurane anesthesia in SD rats. Immunohistochemical analysis for changes in Iba1 (a microglia marker) and GFAP (an astrocyte marker), were performed 5 days after CFA injection. Subcutaneous injection of CFA produced increases in Iba1 and GFAP expression, in the ipsilateral superficial lamia I and II in the medullary dorsal horn of rats. Subcutaneous treatment with BoNT-A attenuated the up-regulation of Iba1 and GFAP expressions induced by CFA injection. Moreover, intracisternal injection of BoNT-A also attenuated the up-regulated Iba1 and GFAP expressions. These results suggest that the anti-nociceptive action of BoNT-A is mediated by modulation activation of glial cells, including microglia and astrocyte.