http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Yongx2010,Tae,Lee, Yex2010,Ryun,Jin, Jianming,Han, Kap‐,Hoon,Kim, Hun,Kim, Jinx2010,Cheol,Lee, Theresa,Yun, Sungx2010,Hwan,Lee, Yinx2010,Won Blackwell Science Ltd 2005 Molecular microbiology Vol.58 No.4
<P><B>Summary</B></P><P>Zearalenone (ZEA) is a polyketide mycotoxin produced by some species of <I>Gibberella/Fusarium</I> and causes hyperestrogenic syndrome in animals. ZEA occurs naturally in cereals infected by <I>Gibberella zeae</I> in temperate regions and threatens animal health. In this study, we report on a set of genes that participate in the biosynthesis of ZEA in <I>G. zeae</I>. Focusing on the non‐reducing polyketide synthase (PKS) genes of the <I>G. zeae</I> genome, we demonstrated that <I>PKS13</I> is required for ZEA production. Subsequent analyses revealed that a continuous, 50 kb segment of DNA carrying <I>PKS13</I> consisted of three additional open reading frames that were coexpressed as a cluster during the condition for ZEA biosynthesis. These genes, in addition to <I>PKS13</I>, were essential for the ZEA biosynthesis. They include another <I>PKS</I> gene (<I>PKS4</I>) encoding a fungal reducing PKS; zearalenone biosynthesis gene 1 (<I>ZEB1</I>), which shows a high similarity to putative isoamyl alcohol oxidase genes; and <I>ZEB2</I> whose deduced product carries a conserved, basic‐region leucine zipper domain. <I>ZEB1</I> is responsible for the chemical conversion of β‐zearalenonol (β‐ZOL) to ZEA in the biosynthetic pathway, and <I>ZEB2</I> controls transcription of the cluster members. Transcription of these genes was strongly influenced by different culture conditions such as nutrient starvations and ambient pH. Furthermore, the same set of genes regulated by <I>ZEB2</I> was dramatically repressed in the transgenic <I>G. zeae</I> strain with the deletion of <I>PKS13</I> or <I>PKS4</I> but not in the <I>ZEB1</I> deletion strain, suggesting that ZEA or β‐ZOL may be involved in transcriptional activation of the gene cluster required for ZEA biosynthesis in <I>G. zeae</I>. This is the first published report on the molecular characterization of genes required for ZEA biosynthesis.</P>
Evaluation of <i>Plasmodium vivax</i> ELISA for the blood screen
Nam, Myungx2010,Hyun,Kim, Jang Su,Cho, Chi Hyun,Han, Eun Taek,Lee, Won Ja,Lee, Hee Kyung,An, Seong Soo A.,Lim, Chae Seung,Lee, Kap No Blackwell Publishing Ltd 2010 Tropical medicine & international health Vol.15 No.12
<P><B>Summary</B></P><P> <I>Plasmodium vivax</I> malaria is the indigenous strain in the Republic of Korea (ROK). <I>Plasmodium vivax</I> can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood‐related products in ROK. We evaluated a <I>P.?vivax</I>‐specific enzyme‐linked immunosorbent assay (Genedia Malaria Ab ELISA 2.0, Green Cross, ROK) with blood samples from four groups: 251 samples from <I>P.?vivax</I>‐infected patients, 39 samples from post‐treatment patients upon follow‐up, 200 samples from healthy volunteers and 421 samples from domestic travellers to and from high endemic areas of ROK. The positive cases from the ELISA test were confirmed by both Giemsa microscopic and polymerase chain reaction methods. The clinical sensitivity and specificity of detecting <I>P.?vivax</I> with ELISA test were 94.4% and 99.0%, respectively. Thirteen of 421 domestic travellers (3.0%) to endemic areas tested positive. The results indicate the effectiveness of detecting antibodies against <I>P.?vivax</I> in blood with Genedia Malaria Ab ELISA 2.0 test in a large blood screen setting.</P>