화상을 입힌 생쥐의 흉선과 비장 임파구아형과 조직학적 소견의 변화

한기택,안상태,강윤섭,임 풍 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4

Due to various documented abnormalities of cell mediated immunity, sepsis still remains the major cause of morbidity and mortality after initial survival from extensive thermal burn. However, the exact mechanisms of immunosuppression after extensive thermal burn remains uncertain. For th observation of immune state, changes of lymphocyte subpopulation have been usually measured in lymphocyte samples from peripheral blood by many reports, but it is only a small fraction of the total lymphocyte pool in the body, and may not present valid picture of the immunological state of the individual. Therefore, analysis of lymphocyte phenotype in thymus and spleen is thought to be necessary in understanding more exact mechanism of immunological changes in extensive thermal burn. The aim of this study is to evaluate the changes of immunocompetency by observing the changes of subpopulation of spleen and thymus lymphocyte using monoclolnal antibodies and changes of tissue with light microscope in burned mice. Cell populations were isolated from spleen and thymus of control and burned mice on the 1st, 3rd, 5th, 7th, 14th and 21st postburn days(PBD). The cell populations were measured with monoclonal antibody by flow cytometry. The results were as follows: 1.In thymus, Thy 1.2+, L3T4+ and LYt2+ cells decreased markedly on the 3rd PBD. Thy 1.2+ and L3T4+ cells decreased also on 5th, 7th, 14th PBD and returned to control level on 21st PBD, but Lyt2+ cells increased more significantly than control after 3rd PBD and returned to normal on 21st PBD. Qa2+ cells revealed most significant reduction on 7th PBD. 2.In spleen, Thy 1.2+, L3T4+ and Ia+ cells increased on 3rd PBD and then decreased on 7th and 14th PBD. Lyt2+ cells increased in all the burned groups. Qa2+ cells increased on 3rd, 5th PBD and returned to normal level. L3T4+ cell/Lyt2+ cell ratio revealed signicant reduction, but they recovered by the 21st PBD. 3.In histopathologic findings of the spleen & thymus, the all cell subpoulations decreased at 1st PBD, but more increased than that of group of 3rd PBD. And then they revealed normal architecture after 5th PBD.

마우스 종양발생에서 Nitric Oxide의 역할에 관한 연구 Ⅲ : Helicobacter pylori에 의해 유발된 마우스 위암에서 염증매개인자의 역할 The role of inducible nitric oxide synthase and cyclooxygenase-2 in H. Pylori-associated gastric carcinogenesis

남기택,오상연,조현무,이국경,강진석,제정환,최미나,한상욱,김대용,장동덕,양기화,안병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

feficotorfer fyf☞ri(Hp)가 위암파 관련이 있다는 역학적인 증거는 많이 있지만 이에 대한 정확한 기전에 대해선 밝혀져 있지 않고 있으며 실험동물 모델도 적절하지 못한 것으로 알려져있다. 본 실험에서는 위암의 원인으로 알려져 있는 f, fyforf'(Hp)를 이용하여 마우스에서 위암 모델을 확립하고 만성염증과정 중에 생성되는 리0와 COX-2 등의 발현이 위암발생에 미치는 명향을 통하여 예방과 치료를 위한 점근을 시도하고자 하였다. 마우스를 7군으로 나누어서 1, 2, 3, 4군의 등물은 MNU를 증류수에 200ppm 농도로 음수병득 이용하띤 10주간 격주로 투여하였으며 MHU 음술 투여 1주 휴씩 후 배양한 f. fyrofi 를 약 109cru/rfll 로 맞춰 한 마리당 0.1ml 씩 이틀 간격으로 세 번에 걸쳐 하룻방 금식시킨 1, 2, 3, 5, 6, 7군기 마우스의 위장에 투입하떴다. 균 투입을 마친 후 다응 날부터 2군쏙 6군은 iNOS 억제제인 aminoguanidine(AG)을 음수병으로 툰여하였으며 3군과 7군은 COX-2 척제제인 nimesulide(NSD)를 투여하였다. 위의 종양발생양상을 샅최보면 bfNU와 Hp만을 투여한 1 관 ; (hfNU +Hp), 2군 : iNO을 inhibitor 투여군(MNU+HP+AG'1. 3군 ;CO딘-2 Inilibitor 투여군(MNU누Hp누 NSD), 4군 ,MNlf 단독투여군, 5군 ;Hp 단독투여춘, 6군 ; 러p 단독에 AG투여군, 7군 , Hp 단독에 NSD투여군의 종양발샐을은 각각 쁜.Bff(l1/16), 70.6%f12/ti), 했.9ff(7/18), 10%(1/10), Off(O/IS)0%(O/S), 0%(O/5)의 발쟁율을 보여 iNOS 억제제인 AC은 좁양발생을 억계하지 못하였으며, COX-2억제제인 NSD 는 종양발생을 유의적으로 감소시켰다. 콩양발생개수에서는 2.62±0.36, 1.41츠0.14, 0.44 르0.12, 0.10±0.10을 보여 AC와 NSD에서 유의성 있게 발생개수를 줄였다. Hp 단독투여에 의해즌는 종양발생이 나타나지 않았으며 HP+AG, Hp+ IfSD 추여군에콕 시험증료 시점에 약물에 의해 Hp의 제균효과가 있는지의 여부를 확인끓기 위하여 PCR을 이용하여 확인한 결과 모두 양성인 것으로 나타나 Hp의 제균효과에 의한 촐양발생 억제가 일어나지는 않았다. 위의 결과로 볼 때 Hp는 위암발생을 촉진하는 것으로 나타났고 딘p 감염시 매개되는 염증인자를 억제하였을 때 종양발생을 억제하는 것으로 위암 발생에서 염증매개인자는 종양을 촉진하는 것으로 나타났으며 it,705 억제제쓱 COX-』 억제제의 위알 예밭효과fl는 효과적일 것으로 사료된다 In spite of a large volume of epidemiological evidence indicating significant relationship between H. pylori infection and gastric adenocarcinoma, a doubt still exists on an elevated risk of stomach cancer by H. pylori infection due to lack of direct evidence of their exact mechanistic link. It is, therefore, essential to have an appropriate animal model for detailed analysis of the role of H. pylori played in gastric carcinogenesis. There is a wealth of evidence to support that over production of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) is involved in the pathogensis of various cancer in both rodents and humans. The aim of this study was to establish a mouse model for H. pylori-associated gastric carcinogenesis and to identify the role of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) played during the gastric carcinogenesis in mice. Eighty-three specific pathogen free, six-week-old male C57BL/6 mice were randomly divided into seven groups. Animals of the group 1, 2, 3, 4 were given MNU in their drinking water at the concentration of 200 p.p.m. for total five cycles of one-week regimen with one-week pause. After completion of MNU administration, they were given autoclaved distilled water for one weeks, and groups 1, 2, 3, 5, 6, 7 were inoculated with H. pylori. After completion of H. pylori. inoculation, groups 2 and 6 were given aminoguanidine in their drinking water at concentration of 1000p.p.m. and animals of group 3 and 7 were given the diet containing 200 ppm nimesulide at 12 weeks of age. All animals were killed at 50 weeks of age. The incidences of the glandular stomach tumors in the group 1, 2, 3 and 4 were 87.5%(14/16), 76.4%(13/17), 44.4(8/18), 10.0%(1/10), respectively and the tumor incidence of group 3(MNU→Hp+nimesulide) was significantly lower than those of group 1(MNU→Hp) at the value of P<0.01. The average numbers of tumors of group 2(MNU→Hp+AG : 1.41±0.24) and group 3(MNU→Hp+nimesulide : 0.44±0.12) were significantly lower than those of group 1(MNU→Hp : 2.62±0.36) at the value of P<0.05. Therefore, overproduced iNOS and COX-2 plays an important role in mice gastric carcinogenesis. We concluded iNOS and COX-2 inhibitor have good effects on gastric carcinogenesis.

濟州地方에서 감자栽培法 改善에 關한 硏究 : I. Gibberellic Acid處理가 겨울감자의 生育 및 收量에 미치는 影響 I. Effect of Gibberellic Acid Treatment on Growth and Yield of Winter Cultivated Potato

朴庸奉,宋昌訓,韓元琢,金基澤 제주대학교 1991 논문집 Vol.32 No.-

濟州地域 겨울감자 栽培用 種薯 自給率 向上을 위하여 가을감자에 GA處理로 早期休眼打破 效果를 규명코저 3個年間의 試驗結果를 報告한다. 1) 定植時 萌芽長은 慣行區에 比하여 濟州産에 GA處理한 것은 萌芽長이 짧았으나 處理濃度가 높을수록 增加하였다. 2) 出現期는 慣行定植區에 比하여 濟州産 GA處理한 것은 11∼16日程度 늦었으나 無處理區 보다는 13∼18일 정도 빠른편이었다. 3) 草長은 GA處理한 것이 慣行區와 濟州産無處理區보다 길었으나 生育中에 草勢가 軟弱하여 低溫伸長性이 弱한 편이었다. 4) 收量은 GA 4ppm 處利한 것이 無處理보다 128% 增收하는 편이었으나 慣行區보다는 18% 程度 減收하는 傾向을 나타내었다. 以上의 結果로 보아 濟州地域 겨울감자 栽培用 種薯가 大關嶺 9月産으로 全量 確保가 어려울 경우 不足한 것은 濟州가을감자에 GA 4ppm을 30分동안 處理하면 겨울감자 栽培用 種薯로 利用이 可能할것으로 판단되었다 Influence of Gibberellin treatment on growth and yields of potatoes (Solanium tuberosum L.) were examined. The results obtained are summarized as follows; 1) The sprout lengths were decreased by the GA treatment more than usual (the produced in September at alpine level). However, it appeared there was a trend of increase, as related to higher GA concentration. 2) The days to emergence were late in the GA treatment more than the usual degree of 11-16 days. It was earlier than the control of 13-18 days. 3) Plant heights were longer by GA treatment than those of usual practices and of the control. However, during the early growing stage, it shows that low temperature resitance was a trend of weakness. 4) In accordance with the rise in concentration of GA treatment, yields were increased by GA 4ppm treatment about 128% more than by the conreol. On the contrary, GA 4ppm treatment showed a trend of decrease, about 18% less than the usual practice. According to these results, it is possible to use the planting seed of winter cultivated potatoes, if we treated GA 4ppm, for 30 minutes to the control.

한국産 황어의 종묘생산에 관한 연구 1. 産卵習性 및 初期生活史

조재권,오성현,노병율,한경호,성기백,박준택 麗水大學校 産業基術硏究所 1999 産業基術硏究所 論文集 Vol.8 No.-

강원도 양양군에 위치한 남대천과 전라남도 섬진강에 분포하고 있는 황어의 종료생산을 위한 기초자료로 産卵習性과 初期生活史에 관하여 관찰한 결과는 다음과 같다. 황어의 産卵期는 자연상태에서 3∼6月이고, 産卵은 흐르는 하천의 자갈 밑에 하였으며, 受精된 卵은 작은 자갈의 표면에 붙어있었다. 알의 크기는 2.70∼3.20㎜(평균 3.00㎜)로 油球는 없었다. 孵化는 평균 사육수온 10.60℃에서 受精 後 300시간부터 시작되었으며, 312시간만에 완료되었다. 孵化 直後의 仔魚는 전장 6.35∼6.87㎜(평균 10.67㎜)로 난황이 거의 흡수되어 後期仔魚期로 접어들며, 입과 항문이 열려있었다. 孵化 後 7∼9일째 後期仔魚는 전장 11.35∼12.30㎜(평균 11.65㎜)로 등지느러미가 생길 부분이 융기하기 시작하였다. 孵化 後 12∼13일째 개체는 전장 12.65∼13.15㎜(평균 12.80)로 이때부터 Daphnia sp.와 Brachionus plicatilis를 攝餌하였으며, 성장이 다소 빨라졌고, 脊索의 끝부분은 45°위로 굽어져 있었다. 孵化 後 43∼45일째의 개체는 전장이 19.55∼22.85㎜(평균 21.95㎜)로 모든 지느러미 줄기가 정수에 달하여 稚魚期로 이행하였다. 孵化 後 50∼52일째의 稚魚들은 전장이 21.54∼27.35㎜(평균 24.55㎜)로 몸의 형태, 체색 및 옆줄이 완전하게 형성되어 成魚에 닮아 있었다. The spawning behavior and early life history of sea rundace, Tribolodon hakonensis were studied. Spawning of T. hakonensis occurs enmasse on bottoms of stream with a spawning season of March to June. The eggs are attached to the under surface of the gravel. The eggs riped were demersal and adhesive, and their diameter were 2.70∼3.20㎜(mean 3.00㎜). Hatching was started at 300 hours after fertilization at mean water temperature 10.60℃. Newly-hatched larvae were 6.35∼6.87㎜ in total length(TL, Mean 6.65㎜), and mouth and anus were not yet opened. 4∼5 days old larvae transformed to postlarva stage and they were 10.25㎜∼11.15㎜ in TL (mean 10.67㎜). As yolk sac were nearly absorbed, mouth and anus were opened. In 7∼9 days after hatching, total length 11.35∼12.30㎜(mean 11.65㎜). The part of the fin-fold at dorsal fin became high. 12∼13 days old larvae were 12.65∼13.15㎜ in TL(mean 12.80㎜), and they fed Daphnia sp. and Brachionus plicatilis actively. In this time the growth rate slightly increased and tip of notocord was flesed on upward. Aggregate numbers of all fin rays were completed at over 19.55∼22.85㎜ in TL(43∼45 days after hatching), at which time the larvae reached the juvenile stage. 50∼52 days old larvae(21.54∼27.35㎜ in TL) were similar in body from, lateral line and color with adult.

Cyclooxygenase-2 expression in murine gastric cancer promoted by Helicobacter pylori infection

Ki Taek Nam,Sang-Yeon Oh,Beom Seok Han,Cheul Kyu Kim,Wan-Seob Cho,Ki-Dae Park,Mina CHoi,Ki-Baik Hahm,Dae-Yong Kim,Dong Deuk Jang,Ki-Hwa Yang 한국실험동물학회 2004 한국실험동물학회 학술발표대회 논문집 Vol.2004 No.-

Oral Presentation : Decreased Helicobacter Pylon-Associated Gastric Carcinogenesis in Mice Lacking Inducible Nitric Oxide Synthase

( Ki Taek Nam ),( Sang Yeon Oh ),( Byeong Woo Ahn ),( Beom Seok Han ),( Chuel Kyu Kim ),( Wan Seob Cho ),( Ki Dae Park ),( Dong Deuk Jang ),( Ki Hwa Yang ),( Dae Yong Kim ) 한국수의병리학회 2004 학술대회 Vol.2 No.-

Comparative Analysis of Therapeutic Radiation by CLINAC using Alanine/ESR

Ki-Taek Han,Chul Hee Min,Hyojun Park,Woo Sang Ahn,Sung Jin Noh 한국자기학회 2023 한국자기학회 학술연구발표회 논문개요집 Vol.33 No.1

Decreased Helicobacter Pylon-Associated Gastric Carcinogenesis in Mice Lacking Inducible Nitric Oxide Synthase

( Ki Taek Nam ),( Sang Yeon Oh ),( Byeong Woo Ahn ),( Beom Seok Han ),( Chuel Kyu Kim ),( Wan Seob Cho ),( Ki Dae Park ),( Dong Deuk Jang ),( Ki Hwa Yang ),( Dae Yong Kim ) 한국수의병리학회 2004 학술대회 Vol.8 No.-

Changes of Lymphocyte Subpopulations in Spleen and Thymus after severe Thermal Injury in Mice

Han, Ki Taek,Ahn, Sang Tae,Lim, Poong CATHOLIC MEDICAL CENTER 1992 Bulletin of the Clinical Research Institute Vol.20 No.1

Sepsis still remains the major cause of morbidity and mortality after extensive thermal injury. This has been attributed to various abnormalities of the impaired cellular immunity. The aim of this study is to investigate the changes of immunocompetency by observing the changes of subpopulations of spleen and thymus lymphocytes and changes of these tissues with light microscope in burned mice. The result of this study showed that burn injury induces severe alterations in lymphocyte numbers and subpopulation percentage in the lymphoid organs. These changes may be thought due to a redistribution of the cellular elements responsible for generation of immune response.

Histological Change and Flap Survival of Expanded Rabbit Skin

Han, Ki-Taek,Ma, Heung-Soo,Kim, Jin,Cho, Moon-Je Catholic Medical Center 1986 Bulletin of the Clinical Research Institute Vol.14 No.-