구강 편평세포암종에서 E-cadherin 및 β-catenin 발현의 변화 : Correlation with Histologic Features and p53 Expression

서진건,권창석,박진배,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Altered expression of cell adheion molecules is associated with biologic behavior of tumor. The aim of this study is to evaluate the expression pattern of E-cadherin, β-catenin and p53 protein according to histologic grade and invasion pattern of squamous cell carcinoma of the oral cavity and the relationship between E-cadherin, β-catenin and p53 protein expression. Methods and Material : The materials were fifty seven cses of squamous cell carcinoma, and clinicl parameers such as age, sex, tumor location, stage and recurrence were recorded. Histologic review was done based on histologic grade and invasion pattern(nodular vs infiltrative). Immunohistochemical stains for E-cadherin and β-ca tenin were interpreted based on staining pattern as los of membranous expression and cytoplasmic expression and p53 protein expression was defined as positive if more than 10% of nuclei were reactive. Statistical analysis between E-cadherin, β-catenin and p53 protein expressions and histologic grade and invasion pattern and the relationship between E-cdherin, β-catenin and p53 protein expressions were perfomed. Results : There wa positive correlation between higher histologic grade and infiltrative pattern. Loss of membranous expression and cytoplasmic expression of E-cadherin were noted in 33.3% and 57.9%, respectively, and loss of membranous expression of E-cadherin showed increaing tendency in poorly differentiated carcinomas, however, there was no significant difference of cytoplasmic expression rate of E-cadherin according to histoogic grade. Altered expression of E-cadherin was more frequent in poorly differentiated carcinomas. Loss of membranous expression and cytoplasmic expression of E-cadherin were more frequent in carinomas with infiltrative pattern than in carcinomas with nodular pattern, but their diferences were not significant, Loss of membranous expression and cytoplasmic expression of β-catenin were observed in 19.3% and 80.7%, respectively. Loss of membranous expression of β-catenin howed no positive correlation according to histologic grade and invasion pattern, but cytoplasmic expression rate of β-catenin was higher in poorly differentiated carcinomas and in carcinomas with infiltrative pettern. p53 protein expression rate was 45.6%, and showed invreasing tendency in poorly differentiated carcinomas, but no significant relationship with invasion pattern. There was an inverse relationship of loss of membranous expression and cytoplasmic expression of E-cadherin and β-catenin. Altered expression of E-cadherin was related to cytoplasmic expression of β-catenin, however, there were no significant relationship between altered expressions of E-cadherin and β-catenin and p53 protein expression. Conclusion : In squamous cell carcinomas of the oral cavity, altered expression of E-cadherin and β-catenin was more frequent in poorly differentiated carcinomas and in carcinomas with infiltrative pattern and there was positive correlation betwween altered expression of E-cadherin and β-catenin. These findings suggest that altered expressions of E-cadherin and β-catenin may have a role in the development of squamous cell carcinomas with aggressive biologic behavior. but altered explosions of E-cadherin and β-catenin. These findings suggest that altered expressions of E-cdherin and β-catenin my have a role in the development of squmaous cell carcinomas with aggressive biologic behavior. But altered expression of E-cadherin and β-catenin might not be related to p53 protein expression. Further study on genetic mutation related to altered expression of E-cadherin and β-catenin will be needed

Separation of Human Breast Cancer and Epithelial Cells by Adhesion Difference in a Microfluidic Channel

Keon Woo Kwon,Sung Sik Choi,Byungkyu Kim,Se Na Lee,Sang Ho Lee,Min Cheol Park,Pilnam Kim,Sukho Park,Youngho Kim,Jungyul Park,Kahp Y. Suh 대한전자공학회 2007 Journal of semiconductor technology and science Vol.7 No.3

A simple, label-free microfluidic cell purification method is presented for separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and 50nm parallel lines with respect to the direction of flow) were fabricated using UVassisted capillary moulding and included inside a polydimethylsiloxane (PDMS) microfluidic channel bonded onto glass substrate. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. It was found that the adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free detection and separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of 300 ㎕/min.

Separation of Human Breast Cancer and Epithelial Cells by Adhesion Difference in a Microfluidic Channel

Kwon, Keon-Woo,Choi, Sung-Sik,Kim, Byung-Kyu,Lee, Se-Na,Lee, Sang-Ho,Park, Min-Cheol,Kim, Pil-Nam,Park, Suk-Ho,Kim, Young-Ho,Park, Jun-Gyul,Suh, Kahp-Y. The Institute of Electronics and Information Engin 2007 Journal of semiconductor technology and science Vol.7 No.3

A simple, label-free microfluidic cell purification method is presented for separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and 50nm parallel lines with respect to the direction of flow) were fabricated using UV-assisted capillary moulding and included inside a polydimethylsiloxane (PDMS) microfluidic channel bonded onto glass substrate. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. It was found that the adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free detection and separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of $300{\mu}l/min$.

Nanotopography-guided migration of T cells.

Kwon, Keon Woo,Park, Hyoungjun,Song, Kwang Hoon,Choi, Jong-Cheol,Ahn, Hyungmin,Park, Moon Jeong,Suh, Kahp-Yang,Doh, Junsang American Association of Immunologists 2012 Journal of Immunology Vol. No.

<P>T cells navigate a wide variety of tissues and organs for immune surveillance and effector functions. Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to study how nanoscale topographical features affect T cell migration. In this study, we systematically investigated mechanisms of nanotopography-guided migration of T cells using nanoscale ridge/groove surfaces. The velocity and directionality of T cells on these nanostructured surfaces were quantitatively assessed with and without confinement, which is a key property of three-dimensional interstitial tissue spaces for leukocyte motility. Depending on the confinement, T cells exhibited different mechanisms for nanotopography-guided migration. Without confinement, actin polymerization-driven leading edge protrusion was guided toward the direction of nanogrooves via integrin-mediated adhesion. In contrast, T cells under confinement appeared to migrate along the direction of nanogrooves purely by mechanical effects, and integrin-mediated adhesion was dispensable. Therefore, surface nanotopography may play a prominent role in generating migratory patterns for T cells. Because the majority of cells in periphery migrate along the topography of extracellular matrices with much lower motility than T cells, nanotopography-guided migration of T cells would be an important strategy to efficiently perform cell-mediated immune responses by increasing chances of encountering other cells within a given amount of time.</P>

Multiscale Fabrication of Multiple Proteins and Topographical Structures by Combining Capillary Force Lithography and Microscope Projection Photolithography

Keon Woo Kwon,Jong-Cheol Choi,Kahp-Yang Suh,Junsang Doh 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.4

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

Kwon, Keon Woo,Choi, Sung Sik,Lee, Sang Ho,Kim, Byungkyu,Lee, Se Na,Park, Min Cheol,Kim, Pilnam,Hwang, Se Yon,Suh, Kahp Y. GENERAL AND APPLIED CHEMISTRY JOURNALS 2007 LAB ON A CHIP Vol.7 No.11

<P>A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (<I>e.g.</I>, one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 µl min<SUP>−1</SUP>. The fraction of MCF7 cells was increased from 0.36 ± 0.04 to 0.83 ± 0.04 under these optimized conditions.</P> <P>Graphic Abstract</P><P>A label-free microfluidic cell separation and enrichment device is presented here by using cell adhesion as a physical marker. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b710054j'> </P>

Nanotophgraphy-guided migration of T cells

Keon Woo Kwon,Hyoung Jun Park,Kahp-Yang Suh,Junang Doh 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.3

A Rare Case of Lumbar Traumatic Intradiscal Hematoma Followed by Repeatative Occupation Related Minor Trauma

( Woo-keun Kwon ),( Jong-keon Oh ),( Taek-hyun Kwon ),( Youn-kwan Park ),( Hong Joo Moon ),( Joo-han Kim ) 대한외상학회 2018 大韓外傷學會誌 Vol.31 No.1

A case of surgically treated intervertebral disc extrusion with intraoperatively confirmed intradiscal hematoma in a 30-year-old physical trainer is presented. Preoperative magnetic resonance imaging revealed downward migrating disc herniation, without definite suggestive findings of intradiscal hematoma. Intervertebral disc herniation with concomitant intradiscal hematoma is extremely rare, but could occur in patients who have excessive axial stress to the spine occupationally. In our case, the patient was an occupational physical trainer who had repetitive minor trauma to the lumbar spine. Although the patient did not have any clear history of major trauma to the spine, the intraoperative findings revealed intradiscal hematoma, which is very rare. The presence of intradiscal hematoma is to be suspected even when preoperative imaging studies shows indefinite findings of hematoma, considering the change in signal intensity of hematoma by time.

Non-Operatively Treated Thoracolumbar Burst Fracture with Posterior Ligamentous Complex Injury: Case Report and Consideration on the Limitation of Thoracolumbar Injury Classification and Severity (TLICS) Score

( Woo-keun Kwon ),( Jong-keon Oh ),( Jun-min Cho ),( Taek-hyun Kwon ),( Youn-kwan Park ),( Hong Joo Moon ),( Joo Han Kim ) 대한외상학회 2018 大韓外傷學會誌 Vol.31 No.2

Fractures at the thoracolumbar region are commonly followed after major traumatic injuries, and up to 20% of these fractures are known to be burst fractures. Making surgical decisions for these patients are of great interest however there is no golden standard so far. Since the introduction of Thoracolumbar Injury Classification and Severity (TLICS) score in 2007, it has been widely used as a referential guideline for making surgical decisions in thoracolumbar fractures. However, there is still limitations in this system. In this clinical case report, we introduce a L1 burst fracture after motor vehicle injury, who was successfully treated conservatively even while she was graded as a TLICS 5 injury. A case report is presented as well as discussion on the limitations of this grading system.

마이크로채널 내에서 부착력 차이에 의한 유방암세포의 선별 및 농축

권건우(Keon Woo Kwon),박민철(Min Cheol Park),김필남(Pilnam Kim),황세연(Se Yon Hwang),이상호(Sang Ho Lee),김병규(Byungkyu Kim),서갑양(Kahp Y. Suh) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.10

A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between human breast epithelial cells (MCF10A) and cancer cells (MCF7), flat or nanostructured polymer surfaces (400nm pillars, perpendicular, or parallel line) were constructed on the bottom of PDMS microfluidic channels using UVassisted capillary moulding. The adhesion of MCF10A and MCF7 on each channel was measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e.g., 1, 2, and 4 hrs). It was found that MCF10A cells show higher adhesion than MCF7 cells regardless of surface nanotopography. The optimum separation was found for 2 hours pre-culture on the 400nm perpendicular line pattern at a flow rate of 200 μl/min. The fraction of MCF7 cells was increased from 0.36 ± 0.04 to 0.83 ± 0.04 under these optimized conditions.