The genome of the mesopolyploid crop species Brassica rapa

Wang, Xiaowu,Wang, Hanzhong,Wang, Jun,Sun, Rifei,Wu, Jian,Liu, Shengyi,Bai, Yinqi,Mun, Jeong-Hwan,Bancroft, Ian,Cheng, Feng,Huang, Sanwen,Li, Xixiang,Hua, Wei,Wang, Junyi,Wang, Xiyin,Freeling, Michael Nature Publishing Group, a division of Macmillan P 2011 Nature genetics Vol.43 No.10

We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops.

The Key to The Future Development of Interactive Art - Virtual Reality Technology

Wang, Junyi,Wang, Yuanyuan,Zhang, Ning,Lee, Eung-Joo,Yabin, Li,Liao, Gang Korea Multimedia Society 2018 The journal of multimedia information system Vol.5 No.4

Interactive art is an art form that closely links art creators and art participants. It has been doomed with advanced technology from the very beginning. Virtual Reality is a technology which born in the background of the maturity of computer technology. Its birth has brought immeasurable possibilities for interactive art, especially the interactivity and multi-sensory experience. This paper discusses the current development status of interactive art in the field of interactive art through the characteristics of virtual reality technology and the future development of this technology will bring to interactive art.

Trapidil determines the fate of RHF rats through inhibition of ER stress

Yilin Wang,Yu Wang,Chengxi Wei,Quan Wan,Zhifei Fan,Liying Xuan,Wanru Geng,Liqun Shao,Jie Long,Junyi Gu,Ming Zhao 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.4

Pulmonary arterial hypertension is a fatal disease,especially when it causes right heart failure (RHF). However, it is difficult to treat. It has been reported thattrapidil (Tra) can improve the redox balance and cardiacconditions. In this study, we investigated the effect of Tra onRHF induced by monocrotaline (MCT) in rats. Male Wistarrats were treated with MCT or Tra. Treatment lasted 28 days,then rats were euthanized after echocardiography and catheterization. Subsequently, lungs and right ventricular myocardiawere evaluated by hematoxylin and eosin, Masson,and TUNEL staining. Protein expression was detected bywestern blotting. We found remarkably expanded rightventricle end-diastolic volume, decreased partial pressureof oxygen (PaO2), increased partial pressure of carbondioxide (PaCO2), right ventricular systolic pressure, meanpulmonary arterial pressure, lung/body weight, and liver/body weight in the RHF rat group, as well as increases inthe apoptosis rate and the expression of endoplasmic reticulumstress (ERS)-related proteins. However, these changes were significantly inhibited by Tra. Our data suggested thatinhibition of ERS is essential for improving RHF, and thattherapeutic intervention of Tra in RHF rats works by reducingERS.

The mechanism of inhibitive effect on hydrogen permeation of X70 steel by lanthanum microalloying: Enhanced kinetics of desorption

Zhengyi Xu,Pengyuan Zhang,Bo Zhang,Bing Lei,Zhiyuan Feng,Junyi Wang,Yawei Shao,Guozhe Meng,Yanqiu Wang,Fuhui Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

In this work, electrochemical hydrogen permeation tests were carried out to obtain the steady-statehydrogen permeation current of X70 pipeline steel in acid environment and alkaline electrolyte withapplied cathodic overpotentials simulating the service conditions of pipelines. It is shown that Lamicroalloyingcan efficiently reduce the steady-state hydrogen permeation current density while maintainingthe same mechanical performance. The experimental permeation data were fitted with Iyer-Pickering-Zamanzadeh (IPZ) and surface effect model to analyze the inhibitive mechanism on hydrogenpermeation, and the results depicted that La element on the material surface greatly facilitates the desorptionprocess of hydrogen atoms. In addition, the calculated hydrogen diffusion coefficient was foundsmaller after La microalloyed, which can be ascribed to the larger hydrogen trap density. It is suggestedthat La-microalloying can mitigate hydrogen damage of X70 pipeline steel in both acidic environment orunder cathodic protection.

Push-Out Tests on Interlocked Angles Connectors in Steel-Concrete-Steel Composite Structure

Junyi Chen,Yonghui Wang,Ximei Zhai,Xudong Zhi,Menghan Sun 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.2

This paper reported a new interlocked angle connector (IAC) for steel-concrete-steel sandwich structures. Shear performances of IACs embedded in normal concrete were studied via a push-out testing program, and the failure mode, shear resistance, and load–slip responses of IACs in normal concrete were obtained. The influences of height, width, thickness, orientation of steel angles, and interlocking bolts on shear behaviours of IACs were experimentally studied. The experimental results indicated that the ultimate shear resistances and slip capacities of IACs were improved via increasing the height, width and thickness of steel angles, while the orientation of steel angles exhibited limited influence on the ultimate shear resistances and failure modes of IACs. In addition, the analytical models were proposed for predicting ultimate shear resistances and load–slip behaviours of IACs. The experimental results were employed to validate the analytical models, and the proposed analytical models were found to provide more accurate predictions on ultimate shear resistances and load–slip behaviours of IACs as compared to the existing design codes.

Effect of Degumming Methods on the Degradation Behavior of Silk Fibroin Biomaterials

Luping Wang,Zuwei Luo,Qiang Zhang,Yupin Guan,Junyi Cai,Renchuan You,Xiufang Li 한국섬유공학회 2019 Fibers and polymers Vol.20 No.1

Degradation behavior is a key consideration in the field of silk fibroin (SF) biomaterials. Degumming to remove sericin is a prerequisite for SF purification; however, the impact of degumming on the degradation behavior of SF biomaterials has not been established. In this study, two different degumming systems, Na2CO3 and NaHCO3, were used. Na2CO3 exhibited higher degumming efficiency but caused greater degradation of the fibroin. The results demonstrated that NaHCO3 degumming could afford regenerated SF with higher molecular weight, resulting in SF films with higher mechanical strengths. The enzymatic degradation behaviors indicated that the SF films prepared by the Na2CO3 degumming process showed faster degradation, revealing that the choice of degumming method has a substantial impact on the biodegradation of SF-based materials. The results showed that manipulating the degumming conditions can be used to tune the molecular weight of the SF, in turn providing control over the degradation rate of SF biomaterials.

Stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats by inhibiting endoplasmic reticulum stress

Yang, Zhijie,Wang, Chun,Zhang, Xia,Li, Jing,Zhang, Ziqi,Tan, Zhao,Wang, Junyi,Zhang, Junyang,Bai, Xiaofeng The Korean Pain Society 2022 The Korean Journal of Pain Vol.35 No.4

Background: The treatment of trigeminal neuralgia remains a challenging issue. Stem cells from human exfoliated deciduous teeth (SHED) provide optimized therapy for chronic pain. This study aimed to investigate the mechanisms underlying the attenuation of trigeminal neuralgia by SHED. Methods: Trigeminal neuralgia was induced by chronic constriction injury of the infraorbital nerve. The mechanical threshold was assessed after model establishment and local SHED transplantation. Endoplasmic reticulum (ER) morphology and Caspase12 expression in trigeminal ganglion (TG) was evaluated as well. BiP expression was observed in PC12 cells induced by tunicamycin. Results: The local transplantation of SHED could relieve trigeminal neuralgia in rats. Further, transmission electron microscopy revealed swelling of the ER in rats with trigeminal neuralgia. Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. Additionally, SHED decreased the up-regulated expression of Caspase12 mRNA and protein in the TG of rats caused by trigeminal neuralgia after chronic constriction injury of the infraorbital nerve mode. Conclusions: This findings demonstrated that SHED could alleviate pain by relieving ER stress which provide potential basic evidence for clinical pain treatment.

Optimization Mechanisms of Microstructure and Mechanical Properties of SiC Fiber Reinforced Ti/Al3Ti Laminated Composite Synthesized Using Titanium Barrier

Chunfa Lin,Siyu Wang,Haoran Yan,Yuqiang Han,Junyi Zhu,Hao Shi 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2

A novel structure-optimized SiC fiber reinforced metal-intermetallic-laminated composite (SiCf-Ti/Al3Ti) without intermetalliccenterline defect has been fabricated by vacuum hot pressing using stacked fibers and foils as well as Ti barrierlayer. Through microstructure characterization by SEM and EBSD, the mechanisms of centerline formation and structuraloptimization were investigated detailedly. The mechanical properties and fracture behaviors of the optimized and nonoptimizedSiCf-Ti/Al3Ti composites were studied via quasi-static compression tests. The experimental results indicatedthat the intermetallic centerline region existing at the mid-plane of Al3Tilayer in non-optimized composite mainly containsnewly-formed Kirkendall voids and gathered metallic oxides. Additionally, owing to the similar moving trails of fibers,oxides and voids in molten Al during hot pressing, SiC fiber is always accompanied with centerline, which causes the poorbonding of SiCf/Al3Ti interface. Unlike that, due to the adding of Ti barrier layer, SiC fibers are separated from centerlineand metallurgically bonded with Al3Tiintermetallic in the optimized composite. The compression testing results proved thatthe optimized SiCf-Ti/Al3Ti composite possesses superior strength and toughness compared with those of the non-optimizedcomposite. Besides, the extending of cracks along centerline often leads to large-scale centerline splitting and untimelySiCf/Al3Ti interface debonding in non-optimized composite. Nevertheless, cracks formed in optimized composite tend topropagate at the interfacial zone between layers instead of cutting off Al3Tilayer along its mid-plane. Moreover, ascribedto the well-bonded SiCf/Al3Ti interface, SiC fibers play an important role in strengthening and toughening the optimizedSiCf-Ti/Al3Ti composite by fiber bridging mechanism.

Fabrication on Nano Size Graphene for Single Electron Transistor

Hanul Kim,Yuhwan Hyeon,Junyi Wang,Sungwon Moon,Dongmok Whang 한국진공학회 2018 한국진공학회 학술발표회초록집 Vol.2018 No.8

Moxibustion for medical personnel with negative emotion and insomnia during COVID-19 pandemic: A randomized, controlled trial

Li Xiying,Li Xiaojuan,Wang Xian,Yin Xuan,Li Shanshan,Wu Junyi,Ren Xiumei,Zhang Wei,Mi Yiqun,Xu Shifen 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.3

Background: We conducted this randomized controlled trial (RCT) to evaluate the effectiveness and safety of moxibustion at Sanyinjiao (SP6) acupoint for treatment of negative mood and sleep quality in healthcare workers during the 2019 coronavirus disease (COVID-19). Methods: A total of 180 participants were divided in a 1:1 ratio into two groups, the treatment group (for moxibustion) and the control group (for no treatment). The treatment group had a 30-minute moxibustion therapy once a day for two weeks, followed by a two-week follow-up. The Hamilton Anxiety Scale (HAMA) was used to assess the degree of the participants' anxiety, and the Patient Health Questionnaire-9 (PHQ-9) was utilized to examine their depressed condition. The Maslach Burnout Inventory-General Survey (MBI-GS) was used to measure the level of burnout among healthcare workers. To determine the severity of insomnia, the Sleep Dysfunction Rating Scale (SDRS) was utilized. At baseline, week 2, and week 4, all scales were evaluated. Results: Compared to the control group, The treatment group improved more significantly in the HAMA at week 2 (MD = -19.01, 95% CI: -21.89 to -16.14; P<0.001) and at week 4 follow-up visits (MD = -8.96, 95% CI: -11.19 to -6.73; P<0.001). A subgroup study of HAMA scores revealed that position and education had significant impact on treatment effectiveness. During the 2-week intervention period, the treatment group showed more significant improvements in depressive symptoms measured by PHQ-9 (13.00±2.41 vs. 15.60±3.65; P<0.001), work burnout symptoms measured by MBI-GS (MD = -11.88, 95% CI, -15.73 to -8.03; P<0.001), and insomnia symptoms measured by SDRS (MD = -2.45, 95% CI, -4.24 to -0.66; P<0.01). There were no significant adverse effects reported. Conclusion: Moxibustion at SP6 may be an effective treatment to improve anxiety, depression, sleep quality, and quality of life for healthcare workers during COVID-19.