Loss of Pten synergizes with c-Met to promote hepatocellular carcinoma development via mTORC2 pathway

Zhong Xu,Junjie Hu,Hui Cao,Maria G Pilo,Antonio Cigliano,Zixuan Shao,Meng Xu,Silvia Ribback,Frank Dombrowski,Diego F Calvisi,Xin Chen 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Hepatocellular carcinoma (HCC) is a deadly malignancy with limited treatment options. Activation of the AKT/mTOR cascade is one of the most frequent events along hepatocarcinogenesis. mTOR is a serine/threonine kinase and presents in two distinct complexes: mTORC1 and mTORC2. While mTORC1 has been extensively studied in HCC, the functional contribution of mTORC2 during hepatocarcinogenesis has not been well characterized, especially in vivo. Pten expression is one of the major mechanisms leading to the aberrant activation of the AKT/mTOR signaling. Here, we show that concomitant downregulation of Pten and upregulation of c-Met occurs in a subset of human HCC, mainly characterized by poor prognosis. Using CRISPR-based gene editing in combination with hydrodynamic injection, Pten was deleted in a subset of mouse hepatocytes (sgPten). We found that loss of Pten synergizes with overexpression of c-Met to promote HCC development in mice (sgPten/c-Met). At the molecular level, sgPten/c-Met liver tumor tissues display increased AKT and mTOR signaling. Using Rictor conditional knockout mice, we demonstrate that sgPten/c-Met-driven HCC development strictly depends on an intact mTORC2 complex. Our findings therefore support the critical role of mTORC2 in hepatocarcinogenesis. sgPten/c-Met mouse model represents a novel valuable system that can be used for the development of targeted therapy against this deadly malignancy.

Efficient Multicast Routing on BCube-Based Data Centers

( Junjie Xie ),( Deke Guo ),( Jia Xu ),( Lailong Luo ),( Xiaoqiang Teng ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.12

Multicast group communication has many advantages in data centers and thus is widely used by many applications. It can efficiently reduce the network traffic and improve the application throughput. For the multicast application in data centers, an essential problem is how to find a minimal multicast tree, which has been proved to be NP-hard. In this paper, we propose an approximation tree-building method for the minimal multicast problem, named HD(Hamming Distance)-based multicast tree. Consider that many new network structures have been proposed for data centers. We choose three representative ones, including BCube, FBFLY, and HyperX, whose topological structures can be regarded as the generalized hypercube. Given a multicast group in BCube, the HD-based method can jointly schedule the path from each of receiver to the only sender among multiple disjoint paths; hence, it can quickly construct an efficient multicast tree with the low cost. The experimental results demonstrate that our method consumes less time to construct an efficient multicast tree, while considerably reduces the cost of the multicast tree compared to the representative methods. Our approach for BCube can also be adapted to other generalized hypercube network structures for data centers after minimal modifications.

Incremental Auto Regressive Prediction Models with External Variables of Greenhouse Air Temperature for Control Purposes

Zhenfeng Xu,Junjie Chen,Jingxia Zhang,Yuan Liu,Qiaowei Zhang 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.9

The impact of actuators should be considered in the prediction modeling of greenhouse air temperature. In this paper, the operating state of a greenhouse was divided into five sub-states based on the on-off characteristic of actuators. A group of novel incremental auto regressive models with external variables (IARX models) suitable for the five operat-ing sub-states were deduced from the mechanistic modeling of greenhouse air tempera-ture. The new IARX models have fewer coefficients than other known ARX models. In or-der to validate the IARX prediction models, the related environmental factors of a glass greenhouse were measured. The prediction results of the IARX models were compared with two typical ARX models. The maximum prediction errors and the mean square errors of the IARX models, under the three operating sub-states of passive state(all actuators are not working), mechanical ventilation and fan-pad cooling, are 0.1°C, 0.14°C, 0.7°C, and 0°C, 0.3°C, 0.4°C, respectively. The prediction results are much better than those of one compared model, while similar with the other.

The influence of surface lithium residue to the performance of LiNi0.9Co0.05Mn0.05O2 cathode materials

Liu Junjie,Chu Chenxiao,Qin Xianzhong,Meng Weisong,Xu Xinrui,Wang Bo,Cai Feipeng 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.3

High nickel ternary cathode materials (Ni ≥ 90%) have great potential for application as power batteries in electric vehicles and have become a hot spot for research on cathode materials. However, the residual Li on the surface of the high Ni ternary cathode materials prepared by solid-phase sintering is one of the main reasons affecting their electrochemical performance. It is found that Li1 + x(Ni0.9Co0.05Mn0.05)1-xO2 with x = 0.3, which has a discharge-specific capacity of 205.74 mAh g −1 at 2.7–4.3 V, 0.1C and 184.1 mAh g −1 at 1 C, retained 89% of its initial discharge capacity after 100 cycles at 1 C. The discharge-specifi c capacity at 10 C was 153.14 mAh g −1. In addition, the Li residue of NCM90-1.03 was 17,189.959 ppm, and the total alkalinity was 4196.896 ppm. The results by X-ray diff raction (XRD) and scanning electron microscope (SEM) showed that when the Li excess was 0.3%, it was possible to obtain uniform particle size, a wider Li layer, and reduced cation mixing. Therefore, choosing the right amount of excess Li can stimulate the application prospect of NCM90 in new energy vehicles.

The corrosion behaviour of alloy 690 tube in simulated PWR secondary water with the effect of solid diffusing hydrogen

Chen, Junjie,Lu, Zhanpeng,Meng, Fanjiang,Xu, Xuelian,Xiao, Qian,Kim, Ho-Sub,Jang, Changheui Elsevier 2019 JOURNAL OF NUCLEAR MATERIALS Vol.517 No.-

<P><B>Abstract</B></P> <P>High-temperature water corrosion tests were performed to investigate the corrosion behaviors of Alloy 690 TT in simulated PWR secondary water with gaseous hydrogen permeation condition. Double-layer oxide films formed on hydrogen-free and hydrogen-permeating specimens. The thickness of a Cr-rich inner oxide film on the hydrogen-free specimens has no obvious difference from the hydrogen-permeating ones. For hydrogen-free specimens, the outer oxide films were Fe and Ni enriched spinel-type oxide particles. For hydrogen-permeating specimens, outer oxide films were sheet-like or needle-like Cr-rich chromia-type oxides. In summary, the solid diffusing hydrogen affected the morphology and composition of outer oxides.</P> <P><B>Highlights</B></P> <P> <UL> <LI> High temperature water corrosion test was performed in hydrogen permeation condition. </LI> <LI> The thickness of Cr-rich inner films has no obvious change by hydrogen permeation. </LI> <LI> Sheet-like outer oxides on hydrogen-permeating samples unlike hydrogen-free ones. </LI> <LI> Fe and Ni-rich spinel-type outer oxide films formed on hydrogen-free specimens. </LI> <LI> Cr-rich chromia-type outer oxide films formed on hydrogen-permeating specimens. </LI> </UL> </P>

In Situ Fabrication of TiO2/Bi3.64Mo0.36O6.55 Nanocomposite with High Visible-Light-Driven Photocatalytic Efficiency

Zhiyuan Yang,Junjie Wang,Lu Chen,Mengjun Liang,Yulin Xu,Chentao Zou,Xiaoxia Liu,Shuijin Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.5

In this work, we developed a simple hydrothermal method toward the fabrication of TiO2/ Bi3.64Mo0.36O6.55 heterostructure, which had superior photocatalytic performance for degrading of RhB under visible light irradiation. The as-prepared samples were characterized by X-ray diffraction (XRD), field emission scanning electron microscope (FESEM), high-resolution transmission electron microscopy (HRTEM), UV-Vis diffuse reflectance spectroscopy (DRS) and photoelectrochemical measurements. The optimal composite with 15 wt.% TiO2/Bi3.64Mo0.36O6.55 (TBMO3) exhibits a much higher photocatalytic activity than that of Bi3.64Mo0.36O6.55 and P25 by degradation of RhB under visible light irradiation within 20 min. The enhanced performance of TBMO3 is predominantly attributed to the synergistic effect both in the higher surface area and the improved separation of photogenerated charge carriers between the two semiconductors. Recycling experiments indicated that TiO2/Bi3.64Mo0.36O6.55 photocatalysts had excellent cycle performance and stability. The photocatalytic mechanism of nanocomposite photocatalysts was proposed, which is confirmed by the active species trapping experiments and photoluminescence tests.

Design of a High-Precision Constant Current AC-DC Converter with Inductance Compensation

Chang, Changyuan,Xu, Yang,Bian, Bin,Chen, Yao,Hu, Junjie The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

A primary-side regulation AC-DC converter operating in the PFM (Pulse Frequency Modulation) mode with a high precision output current is designed, which applies a novel inductance compensation technique to improve the precision of the output current, which reduces the bad impact of the large tolerance of the transformer primary side inductance in the same batch. In this paper, the output current is regulated by the OSC charging current, which is controlled by a CC (constant current) controller. Meanwhile, for different primary inductors, the inductance compensation module adjusts the OSC charging current finely to improve the accuracy of the output current. The operation principle and design of the CC controller and the inductance compensation module are analyzed and illustrated herein. The control chip is implemented based on a TSMC 0.35μm 5V/40V BCD process, and a 12V/1.1A prototype has been built to verify the proposed control method. The deviation of the output current is within ±3% and the variation of the output current is less than 1% when the inductances of the primary windings vary by 10%.

Improved DV-Hop Localization Algorithm Based on Bat Algorithm in Wireless Sensor Networks

( Yuan Liu ),( Junjie Chen ),( Zhenfeng Xu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.1

Obtaining accurate location information is important in practical applications of wireless sensor networks (WSNs). The distance vector hop (DV-Hop) is a frequently-used range-free localization algorithm in WSNs, but it has low localization accuracy. Moreover, despite various improvements to DV-Hop-based localization algorithms, maintaining a balance between high localization accuracy and good stability and convergence is still a challenge. To overcome these shortcomings, we proposed an improved DV-Hop localization algorithm based on the bat algorithm (IBDV-Hop) for WSNs. The IBDV-Hop algorithm incorporates optimization methods that enhance the accuracy of the average hop distance and fitness function. We also introduce a nonlinear dynamic inertial weight strategy to extend the global search scope and increase the local search accuracy. Moreover, we develop an updated solutions strategy that avoids premature convergence by the IBDV-Hop algorithm. Both theoretical analysis and simulation results show that the IBDV-Hop algorithm achieves higher localization accuracy than the original DV-Hop algorithm and other improved algorithms. The IBDV-Hop algorithm also exhibits good stability, search capability and convergence, and it requires little additional time complexity and energy consumption.

Modified Wettability of Micro-structured Steel Surfaces Fabricated by Elliptical Vibration Diamond Cutting

Jianguo Zhang,Andreas Rosenkranz,Junjie Zhang,Jiang Guo,Xiaoqing Li,Xiao Chen,Junfeng Xiao,Jianfeng Xu 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Hardened steel is an important material related to the development of modern industry branches. In order to satisfy the adhesion strength as well as the corrosion and wear resistance under extreme conditions, the modification of the wettability of hardened steel has become an important scientific topic. As micro/nano-structured surfaces play an essential role to induce advanced functional surfaces with wettability control, this contribution aims at presenting the feasibility of micro/nano sculpturing of hardened steels by elliptical vibration diamond cutting. The influence of the fabricated micro/nano structures on the resulting wettability is discussed and related to the contact line density as well as asperity heights. In this regard, it has been verified that a pitch value of 12 μm and structural height of 500 nm are the preferred structural parameters to increase the hydrophobicity of the textured steel specimens.

Design of a High-Precision Constant Current AC-DC Converter with Inductance Compensation

Changyuan Chang,Yang Xu,Bin Bian,Yao Chen,Junjie Hu 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

A primary-side regulation AC-DC converter operating in the PFM (Pulse Frequency Modulation) mode with a high precision output current is designed, which applies a novel inductance compensation technique to improve the precision of the output current, which reduces the bad impact of the large tolerance of the transformer primary side inductance in the same batch. In this paper, the output current is regulated by the OSC charging current, which is controlled by a CC (constant current) controller. Meanwhile, for different primary inductors, the inductance compensation module adjusts the OSC charging current finely to improve the accuracy of the output current. The operation principle and design of the CC controller and the inductance compensation module are analyzed and illustrated herein. The control chip is implemented based on a TSMC 0.35μm 5V/40V BCD process, and a 12V/1.1A prototype has been built to verify the proposed control method. The deviation of the output current is within ±3% and the variation of the output current is less than 1% when the inductances of the primary windings vary by 10%.