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Wu Jun(Jun Wu),Luo Yuxin(Yuxin Luo) 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.8 No.1
The problem of “shelling” of specialized farmers’ cooperatives is that farmers’ cooperatives neither insist cooperation essence nor farmer actual participation, and there is no normal operation. The “shelling” problem of farmers’ cooperatives in Guizhou Province is manifested in the three phenomena of “titular form”, “be more in name than in reality” and “unsustainable form”. For the unsustainable farmers’ cooperatives, we can take measures such as improving the organizational structure and management system, exploring joint construction mechanism among the rural credit cooperatives, supply and marketing cooperatives and farmers’ cooperatives, exploring technical services, strengthening the connection of interests and cultivating talent through multiple channels to solve the difficult problems existing in “organization, capital, market, talent, motivation” of the farmers’ cooperatives, achieving the high-quality development.
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Research advances in reproduction for dairy goats
Jun Luo,Wei Wang,Shuang Sun 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.8
Considerable progress in reproduction of dairy goats has been made, with advances in reproductive technology accelerating dairy goat production since the 1980s. Reproduction in goats is described as seasonal. The onset and length of the breeding season is dependent on various factors such as breed, climate, physiological stage, male effect, breeding system, and photoperiod. The reproductive physiology of goats was investigated extensively, including hypothalamic and pituitary control of the ovary related to estrus behavior and cyclicity etc. Photoperiodic treatments coupled with the male effect allow hormone-free synchronization of ovulation, but the kidding rate is still less than for hormonal treatments. Different protocols have been developed to meet the needs and expectations of producers; dairy industries are subject to growing demands for year round production. Hormonal treatments for synchronization of estrus and ovulation in combination with artificial insemination (AI) or natural mating facilitate out-of-season breeding and the grouping of the kidding period. The AI with fresh or frozen semen has been increasingly adopted in the intensive production system, this is perhaps the most powerful tool that reproductive physiologists and geneticists have provided the dairy goat industry with for improving reproductive efficiency, genetic progress and genetic materials transportation. One of the most exciting developments in the reproduction of dairy animals is embryo transfer (ET), the so-called second generation reproductive biotechnology following AI. Multiple ovulation and ET (MOET) program in dairy goats combining with estrus synchronization (ES) and AI significantly increase annual genetic improvement by decreasing the generation interval. Based on the advances in reproduction technologies that have been utilized through experiments and investigation, this review will focus on the application of these technologies and how they can be used to promote the dairy goat research and industry development in the future.
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Intrusion Detection System based on Hidden Conditional Random Fields
Jun Luo,Zenghui Gao 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9
Intrusion detection is an important way to ensure the security of computers and networks. In this paper, a new intrusion detection system (IDS) is proposed based on Hidden Conditional Random Fields (HCRFs). In order to optimize the performance of HCRFs, we bring forward the Two-stage Feature Selection method, which contains Manual Feature Selection method and Backward Feature Elimination Wrapper (BFEW) method. The BFEW is a feature selection method which is introduced based on wrapper approach. Experimental results on KDD99 dataset show that the proposed IDS not only has a great advantage in detection efficiency but also has a higher accuracy when compared with other well-known methods.
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Luo Jian-jun 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6
A numerical simulation on the 3D compressible viscous unsteady flow field induced by a high-speed subway train, while it’s passing by the platform screen door of subway station, is presented in the paper. The research on transient pressure in tunnel due to changes of positions of platform screen door is proceeded in order to design platform screen doors for underground stations of Dong-guan to Huizhou inter-city rail traffic. Result shows that the platform is subjected to aerodynamic compression and tension cycle loads all the time. And compression load is larger than tension load. The maximum and minimum pressure values in absolute terms got from measuring points of the whole station decrease gradually along the direction train going ,no matter whether the platform screen door is fixed or not. The absolute values of maximum and minimum pressure of piston wind generated at platform screen door are decreasing while the spacing between platform screen door and platform becomes wider. The pressure value of air at exit is larger than that of air at entrance. As for a high-speed subway station, it is a practicable way to set air shafts at entrance and exit of station to mitigate pressure of air in station and tunnel. The pressure value of air could have a 10 to 15 percent reduction if there is an air shaft. Besides, the platform screen door can be installed in half tall of PSD.
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Luo Juan,Bai Luyan,Tao Jun,Wen Yu,Li Mingke,Zhu Yunzhen,Luo Sufeng,Pu Guangyu,Ma Lanqing 한국유전학회 2021 Genes & Genomics Vol.43 No.10
Background Vacuolating cytotoxin (VacA) is an important virulence factor of Helicobacter pylori (H. pylori). It was previously believed that VacA can trigger the cascade of apoptosis on mitochondria to lead to cell apoptosis. Recently, it was found that VacA can induce autophagy. However, the molecular mechanism by which VacA induces autophagy is largely unknown. Objective We aimed to explore the molecular mechanism of autophagy induced by H. pylori in gastric cancer cells and the efect of autophagy on the survival of gastric cancer cells. Methods The autophagy of human gastric cancer cell line SGC7901 was detected by Western blot and RT-PCR in the treatment of VacA protein of H. pylori. The relationship between autophagy and reactive oxygen species (ROS) in the proliferation of gastric cancer cells were studied by gene expression silences (siRNA) and CM-H2DCFDA (DCF) staining. Results The results showed that VacA protein secreted by H. pylori in the supernatant stimulated autophagy in SGC7901 cells. After VacA protein treatment, the mRNA expressions of BECN1, ATG7 and PIK3C3, were up-regulated. ATG7 silencing by siRNA inhibited VacA-induced autophagy. Furthermore, our data demonstrated that VacA protein increased ROS levels. Addition of the antioxidant N-acetyl-l-cysteine (NAC) suppressed the levels of ROS, leading to inhibition of autophagy. Conclusions H. pylori VacA is a key toxin that induces autophagy by increased ROS levels. And our fndings demonstrated that VacA signifcantly inhibited proliferation in SGC7901 cells.
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Jun Luo,Junying Liu,He-he Liu,Tao Zhang,Jiwen Wang,Hua He,Chunchun Han 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7
Objective: The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Methods: Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established. Results: The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds. Conclusion: This data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future.
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Luo, Qiong,Li, Zhuoneng,Yan, Jun,Zhu, Fan,Xu, Ruo-Jun,Cai, Yi-Zhong The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4
Lycium barbarum polysaccharides (LBPs) are important functional constituents in red-colored fruits of L. barbarum (Guo Qi Zi, a well-known traditional Chinese medicinal plant commonly known as Goji berry or wolfberry). The influence of LBP on human prostate cancer cells was systematically investigated in vitro and in vivo. The in vitro effects of LBP on two cell lines (PC-3 and DU-145) were examined by using trypan blue exclusion staining, single-cell gel electrophoresis, flow cytometry, terminal dUTP nick-end labeling assay, and immunohistochemical assay (assessment of Bcl-2 and Bax expression). The in vivo effect of LBP on PC-3 cells was assessed in the nude mouse xenograft tumor model. The in vitro results showed that LBP can dose- and time-dependently inhibit the growth of both PC-3 and DU-145 cells. LBP caused the breakage of DNA strands of PC-3 and DU-145 cells; the tail frequency and tail length were significantly higher than that of control cells. LBP also markedly induced PC-3 and DU-145 cell apoptosis, with the highest apoptosis rates at 41.5% and 35.5%, respectively. The ratio of Bcl-2/Bax protein expression following LBP treatments decreased significantly with a dose. effect relationship, which suggested that LBP can regulate the expression of Bcl-2 and Bax to induce apoptosis of PC-3 and DU-145 cells. The in vivo experimental results indicate that LBP might significantly inhibit PC-3 tumor growth in nude mice. Both the tumor volume and weight of the LBP treatment group were significantly lower than those of the control group.
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