http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sudden cardiac death as a naturally-occurring ventricular hypertrophy in Macaca fascicularis
Hyeon-Gu Yeo,Junghyung Park,Hyun Kuk Kim,Joo Myung Lee,Bon-Sang Koo,Jeong-Hee An,Chang-Yeop Jeon,You Jung An,Won Seok Choi,Sung-Hyun Park,Jincheol Seo,Jinyoung Won,Keonwoo Kim,Jiyeon Cho,Yu Gyeong Kim 충북대학교 동물의학연구소 2021 Journal of Biomedical and Translational Research Vol.22 No.3
고추와 파프리카의 색과 조리방법의 차이에 따른 베타카로틴, 비타민 E, 엽산의 함량과 잔존율
김현영(Hyeon Young Kim),김홍균(Honggyun Kim),천지연(Jiyeon Chun),정혜정(Heajung Chung) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.6
본 연구는 파프리카와 고추 간의 품종별, 색깔별로 나누어 조리방법에 따라 베타카로틴, 비타민 E, 엽산의 함량을 비교하고자 하였다. 또한, 4종류의 시료를 서로 다른 조리방법(삶기, 굽기, 볶기, 튀기기, 찌기, 로스팅, 전자레인지)을 사용하였고, 이를 통해 베타카로틴, 비타민 E, 엽산의 잔존율과 각각의 함량에 대한 색, 종류, 조리방법의 상관성을 조사하였다. 파프리카와 고추는 모두 조리과정에서 중량이 감소하였다. 베타카로틴, 엽산의 함량은 색깔, 종류, 조리방법의 영향을 받았고(P<0.05), 비타민 E의 함량은 영향을 크게 받지 않았다. 조리 전 시료의 베타카로틴, 비타민 E, 엽산 함량(mg/100 g) 범위는 각각 95.4(풋고추)~2,441.1(홍고추), 0.7(녹색 파프리카)~4.2(적색 파프리카), 6.2(풋고추)~148.7(홍고추)로 나타났다. 조리 후 베타카로틴, 비타민 E, 엽산의 함량은 각각 튀긴 홍고추, 로스팅한 홍고추, 삶은 홍고추에서 가장 높은 함량을 보였다. 잔존율의 경우 베타카로틴, 비타민 E, 엽산이 각각 77.3(홍고추-삶기)~356.4(풋고추-로스팅)%, 2.0(홍고추-전자레인지)~789.3(녹색 파프리카-튀기기)%, 20.9(홍고추-전자레인지)~445.1(풋고추-찌기)% 범위로 나타났다. 홍고추를 제외한 나머지 모든 시료에서 베타카로틴, 비타민 E, 엽산이 조리방법에 상관없이 75% 이상의 높은 잔존율을 보였다. 홍고추의 비타민 E와 엽산은 튀기기와 전자레인지에서 2.0~32.1% 범위로 낮은 잔존율을 보였다. 이러한 결과들을 종합해 보았을 때 파프리카와 고추의 조리 전후에 대한 기초자료로 활용이 가능할 것으로 생각하며, 전자레인지를 이용한 조리방법에 따른 영양소 파괴에 대한 추가적인 연구가 필요할 것으로 생각한다. β-Carotene, vitamin E, and folate contents according to color and species of Capsicum annuum were investigated. In addition, four C. annuum species were cooked by using different methods (boiling, pan-cooking, pan-frying, deep-frying, steaming, roasting, and microwaving), and retention rates of β-carotene, vitamin E, and folate affected by color and species were evaluated. Weight loss was observed in all paprika and pepper species using all cooking methods. β-Carotene and folate contents of C. annuum samples were significantly different by color, species, and cooking method (P<0.05), whereas vitamin E contents were not significantly affected by species. β-Carotene, vitamin E, and folate contents (mg/100 g) of raw C. annuum showed ranges of 95.4 (green pepper)∼2,441.1 (red pepper), 0.7 (green paprika)∼4.2 (red paprika), and 6.2 (green pepper)∼148.7 (red pepper). As a result, the highest β-carotene, vitamin E, and folate contents were detected in deep fried red pepper, roasted red pepper, and boiled red pepper, respectively, among the cooked samples. Retention rates of C. annuum varied by cooking method, resulting in ranges of 77.3% (boiled red pepper)∼356.4% (roasted green pepper) for β-carotene, 2.0% (microwaved red pepper)∼789.3% (deep-fried green paprika) for vitamin E, and 20.9% (microwaved red pepper)∼445.1% (steamed green pepper) for folate. Over 75% retention rates were observed for β-carotene, vitamin E, and folate in all samples except for red pepper. However, in red pepper, vitamin E and folate retentions were remarkably lowered by deep frying and microwaving, resulting in rates of 2.0∼32.1%. This study provides information on effective cooking methods for vitamin retention in C. annuum depending on color and species.
Neural Stem Cell Differentiation Using Microfluidic Device-Generated Growth Factor Gradient
( Ji Hyeon Kim ),( Jiyeon Sim ),( Hyun-jung Kim ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.4
Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.
정지연 ( Jiyeon Jeong ),이경현 ( Kyunghyun Lee ),최은진 ( Eun-jin Choi ),김지현 ( Ji-hyeon Kim ),소병재 ( Byungjae So ),이승희 ( Seunghee Lee ),신현호 ( Hyunho Shin ),정지열 ( Ji-youl Jung ) 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.1
An 11 year-old male Korean short-haired cat was presented to local animal hospital due to weight loss, vomiting, and intestinal hypomotility. After the cat was euthanized by poor clinical outcomes, necropsy was performed at Animal and Plant Quarantine Agency. At necropsy, the stomach was enlarged and had some nearly complete pellet food and the yellow mucous contents. The lumen of the middle and lower parts of the jejunum became narrow. Histopathologically, medium-sized lymphoid cells with hyperchromatic nuclei enclosed by scant cytoplasm were diffusely proliferated from mucosa to serosa of the small intestine. These findings were mainly observed in the jejunum and slightly in the duodenum and ileum. The monomorphous lymphocytes were 1 to 1.5 times larger than red blood cells and had few mitotic figures. Metastasis of the tumor cells to other organs was not observed. In the result of immunohistochemical analysis for identifying the origin of tumor cells, CD3 was expressed, but CD79 was not detected in the infiltrated cells. This case was diagnosed as T cell intestinal lymphoma in a Korean short-haired cat based on the clinical signs, gross findings, histopathology, and immunohistochemistry.
Park Jiyeon,Kim Dong-Moung,Lee Jin-Ok,Park Hyeon-Chun,Ryu Brian Y.,Kim Ju Han,Lee Sug Hyung,Chung Yeun-Jun 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Despite growing evidence of the relevance of alternative splicing (AS) to cancer development and progression, the biological implications of AS for tumor behaviors, including papillary thyroid cancer (PTC), remain elusive. With the aim of further understanding the molecular and histological subtypes of PTC, we in this study explored whether AS events might act as new molecular determinants. For this purpose, AS profiles were analyzed in RNA-sequencing data from The Cancer Genome Atlas (TCGA) and from a Korean patient dataset. A total of 23 distinct exon-skipping (ES) events that correlated significantly with PTC oncogenic activity and differentiation scores were identified. The two top-ranked ES events, NUMA1_17515 in exon 18 of NUMA1 and TUBB3_38175 in exon 6 of TUBB3, showed high correlations with oncogenic activities and discriminated histological and molecular subtypes of PTC. Furthermore, two novel intron-retention (IR) events for TUBB3 were uncovered. All ES and IR events for the TUBB3 gene were predicted to induce nonsense-mediated mRNA decay. The relative abundances of intron reads in the PTC dataset from TCGA showed IR levels to differ significantly among PTC subtypes, possibly reflecting their different tumor behaviors. This study provides a landscape of AS changes among PTC subtypes and identified two significant AS events, NUMA1_17515 and TUBB3_38175, as potential AS biomarkers for PTC subclassification and characterization. The AS events identified in this study may be involved in the development of phenotypic differences underlying the functional characteristics and histological differentiation of PTCs.