Effects of Aqueous Extract of Schizandrae Fructus on Lead-Induced Change of Monoamine Neurotransmitters in Hippocampus

Zhao, Rong Jie,Zhao, Zheng Lin,Zhao, Xiu Feng,Zhao, Guang Wen,Li, Meng Quan,Wu, Yi Yan,Li, Jing Qiu,Guan, Li Xin,Kim, Sang-Chan The Korean Medicine Society for the Herbal Formula 2009 大韓韓醫學方劑學會誌 Vol.17 No.2

The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.

Synthesis, Characterization and Property Studies on a Dinuclear Copper(II) Complex with Dipyridine Derivate and Acetylacetone

Zhao, Pu Su,Guo, Zhi Yan,Sui, Jing,Wang, Jing,Jian, Fang Fang Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1

A dinuclear copper(II) complex of [$Cu_2(aceace)_4$(dipyph)] [aceace = acetylacetone, dipyph = 1,4-di(4-pyridylethene-2-yl-)benzene] has been synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction. It crystallizes in the monoclinic system, space group P21/c, with lattice parameters a = 7.9584(16) $\AA$, b = 18.594(4) $\AA$, c = 15.063(4) $\AA$ $\beta=120.97(2)^o$ and $M_r$ = 807.85 ($C_{40}H_{44}Cu_2N_2O_8$), Z = 2. Each of the $Cu^{2+}$ ion adopts a square pyramid geometry and coordinates with four oxygen atoms from two aceace ligands and one nitrogen atom from dipyph bidentate ligand. Magnetic measurement shows that the Weiss constant and Curie constant for the title compound are -0.22 K and 0.1154 emu K/mol, respectively. Thermal stability data indicate that the title complex undergoes two steps decomposition and the residue is $Cu_2O_4$. In the potential range of -1.5 ~ 0.8 V, the title complex represents an irreversible electrochemical process.

Research on the Program-Driven Innovation and Entrepreneurship Education Mode of Local Universities in China

Zhao, Jing,Wang, Yuhui,Zhu, Yongjin 부산대학교 중국연구소 2021 Journal of China Studies Vol.24 No.2

Synergistic and sustainable activation of peroxymonosulfate by nanoscale MWCNTs-CuFe2O4 as a magnetic heterogeneous catalyst for the efficient removal of levofloxacin

Zhao Jing,Xiao Pengfei 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.6

Nanoscale CuFe2O4 was anchored on the surface of multiwalled carbon nanotubes (MWCNTs) as a magnetic heterogeneous catalyst to achieve efficient and sustainable activation of peroxymonosulfate and degradation of levofloxacin through the synergistic effect of the above materials. The catalyst properties were characterized by a series of detection techniques. It was found that the mass ratio of MWCNTs-CuFe2O4, operational parameters and common interfering substances influenced the levofloxacin removal efficiency to a certain extent. This study sheds light on the ultraefficient removal of levofloxacin with the MWCNTs-CuFe2O4(1:3)/peroxymonosulfate system, which has advantages over other reaction systems. More importantly, we propose two pathways of peroxymonosulfate activation, including free radicals and nonfree radicals, in which superoxide radicals and signal oxygen are the main active species. In addition, we observed that the MWCNT surface groups contributed to the peroxymonosulfate activation processes with the generation of extra reactive species. The Fe3+/Fe2+ and Cu2+/Cu+ redox cycles are conducive to the continuous generation of active species. The results of the catalyst recycling test, metal ion leaching test and mineralization test suggested that the fabricated catalyst had excellent catalytic stability, sustainability and mineralization ability. In addition, twenty-one intermediates were detected using liquid chromatography-mass spectrometry, and three possible degradation pathways were further proposed. MWCNTs-CuFe2O4 makes up for the shortcomings of transition metals and single carbon materials in activating peroxymonosulfate to treat wastewater and have significant potential to improve the separation and catalytic capacity of the catalyst. This study provides new ideas for the design of high-performance multiphase catalysts for applications in catalytic oxidation and proposes new insights into the mechanistic investigation.

Apoptotic activity of a new jasmonate analogue is associated with its induction of DNA damage

Zhao, Jing,Kang, Saeromi,Zhang, Xin,You, Song,Park, Jang-Su,Jung, Jee H,Kim, Dong-Kyoo Spandidos Publications 2010 ONCOLOGY REPORTS Vol.24 No.3

<P>The current study was undertaken to investigate the effects of methyl 5-chloro-4,5-didehydrojasmonate (J7), an analogue of methyl jasmonate, on the in vitro growth of human cervical carcinoma HeLa cells. Significantly decreased rates of viability (IC50 approximately 15 microM) as well as evidence of apoptosis were observed with J7. Cell morphological changes observed under light microscopy confirmed apoptosis occurrence. Furthermore, the results from Annexin V-FITC/PI double staining and the cell cycle arrest assay indicated that J7 induced earlier apoptosis of HeLa cells. J7 also reduced the expression of Bcl-2 and subsequent activation of a protease cascade involving caspase-9 and -3 by Western blot assay was observed. We also found that J7 was able to induce DNA damage. These findings suggest that J7 induces HeLa cell apoptosis by activation of caspase pathway and the apoptotic effect is associated with DNA damage. Therefore, J7 may be a candidate compound to be developed into an anticancer agent.</P>

Comparison of Fecal Microbiota of Mongolian and Thoroughbred Horses by High-throughput Sequencing of the V4 Region of the 16S rRNA Gene

Zhao, Yiping,Li, Bei,Bai, Dongyi,Huang, Jinlong,Shiraigo, Wunierfu,Yang, Lihua,Zhao, Qinan,Ren, Xiujuan,Wu, Jing,Bao, Wuyundalai,Dugarjaviin, Manglai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.9

The hindgut of horses is an anaerobic fermentative chamber for a complex and dynamic microbial population, which plays a critical role in health and energy requirements. Research on the gut microbiota of Mongolian horses has not been reported until now as far as we know. Mongolian horse is a major local breed in China. We performed high-throughput sequencing of the 16S rRNA genes V4 hypervariable regions from gut fecal material to characterize the gut microbiota of Mongolian horses and compare them to the microbiota in Thoroughbred horses. Fourteen Mongolian and 19 Thoroughbred horses were used in the study. A total of 593,678 sequence reads were obtained from 33 samples analyzed, which were found to belong to 16 phyla and 75 genera. The bacterial community compositions were similar for the two breeds. Firmicutes (56% in Mongolian horses and 53% in Thoroughbred horses) and Bacteroidetes (33% and 32% respectively) were the most abundant and predominant phyla followed by Spirochaete, Verrucomicrobia, Proteobacteria, and Fibrobacteres. Of these 16 phyla, five (Synergistetes, Planctomycetes, Proteobacteria, TM7, and Chloroflexi) were significantly different (p<0.05) between the two breeds. At the genus level, Treponema was the most abundant genus (43% in Mongolian horses vs 29% in Thoroughbred horses), followed by Ruminococcus, Roseburia, Pseudobutyrivibrio, and Anaeroplasma, which were detected in higher distribution proportion in Mongolian horses than in Thoroughbred horses. In contrast, Oscillibacter, Fibrobacter, Methanocorpusculum, and Succinivibrio levels were lower in Mongolian horses. Among 75 genera, 30 genera were significantly different (p<0.05) between the two breeds. We found that the environment was one of very important factors that influenced horse gut microbiota. These findings provide novel information about the gut microbiota of Mongolian horses and a foundation for future investigations of gut bacterial factors that may influence the development and progression of gastrointestinal disease in horses.

Strategies for Constructing Tissue-Engineered Fat for Soft Tissue Regeneration

Zhao Jing,Lu Feng,Dong Ziqing 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.3

Background: Repairing soft tissue defects caused by inflammation, tumors, and trauma remains a major challenge for surgeons. Adipose tissue engineering (ATE) provides a promising way to solve this problem. Methods: This review summarizes the current ATE strategies for soft tissue reconstruction, and introduces potential construction methods for ATE. Results: Scaffold-based and scaffold-free strategies are the two main approaches in ATE. Although several of these methods have been effective clinically, both scaffold-based and scaffold-free strategies have limitations. The third strategy is a synergistic tissue engineering strategy and combines the advantages of scaffold-based and scaffold-free strategies. Conclusion: Personalized construction, stable survival of reconstructed tissues and functional recovery of organs are future goals of building tissue-engineered fat for ATE. Background: Repairing soft tissue defects caused by inflammation, tumors, and trauma remains a major challenge for surgeons. Adipose tissue engineering (ATE) provides a promising way to solve this problem. Methods: This review summarizes the current ATE strategies for soft tissue reconstruction, and introduces potential construction methods for ATE. Results: Scaffold-based and scaffold-free strategies are the two main approaches in ATE. Although several of these methods have been effective clinically, both scaffold-based and scaffold-free strategies have limitations. The third strategy is a synergistic tissue engineering strategy and combines the advantages of scaffold-based and scaffold-free strategies. Conclusion: Personalized construction, stable survival of reconstructed tissues and functional recovery of organs are future goals of building tissue-engineered fat for ATE.

XIAP Associated Factor 1 (XAF1) Represses Expression of X-linked Inhibitor of Apoptosis Protein (XIAP) and Regulates Invasion, Cell Cycle, Apoptosis, and Cisplatin Sensitivity of Ovarian Carcinoma Cells

Zhao, Wen-Jing,Deng, Bo-Ya,Wang, Xue-Mei,Miao, Yuan,Wang, Jian-Nan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Background: X-linked inhibitor of apoptosis protein (XIAP) associated factor 1 (XAF1) exhibits aberrantly low or absent expression in various human malignancies, closely associated with anti-apoptosis and overgrowth of cancer cells. However, limited attention has been directed towards the contribution of XAF1 to invasion, apoptosis, and cisplatin (DDP)-resistance of epithelial ovarian cancer (EOC) cells. This study aimed to evaluate the potential effects of XAF1 on invasion, cell cycle, apoptosis, and cisplatin-resistance by overexpressing XAF1 in SKOV-3 and SKOV-3/DDP cells. Methods and Results: The pEGFP-C1-XAF1 plasmid was transfected into SKOV-3 and SKOV-3/DDP cells, and the expression of XAF1 at both mRNA and protein levels was analyzed by reverse transcription-PCR and Western blotting. Overexpression of XAF1 suppressed XIAP expression in both SKOV-3 and SKOV-3/DDP cells. Transwell invasion assays demonstrated that XAF1 exerted a strong anti-invasive effect in XAF1-overexpressing cells. Moreover, flow cytometry analysis revealed that XAF1 overexpression arrested the cell cycle at G0/G1 phase, and cell apoptosis analysis showed that overexpression of XAF1 enhanced apoptosis of SKOV-3 and SKOV-3/DDP cells apparently by activating caspase-9 and caspase-3. Furthermore, MTT assay confirmed a dose-dependent inhibitory effect of cisplatin in the tested tumor cells, and overexpression of XAF1 increased the sensitivity of SKOV-3 and SKOV-3/DDP cells to cisplatin-mediated antiproliferative effects. Conclusions: In summary, our data indicated that overexpression of XAF1 could suppress XIAP expression, inhibit invasion, arrest cell cycle, promote apoptosis, and confer cisplatin-sensitivity in SKOV-3 and SKOV-3/DDP cells. Therefore, XAF1 may be further assessed as a potential target for the treatment of both cisplatin-resistant and non-resistant EOCs.

A One-Step System for Convenient and Flexible Assembly of Transcription Activator-Like Effector Nucleases (TALENs)

Zhao, Jinlong,Sun, Wenye,Liang, Jing,Jiang, Jing,Wu, Zhao Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.9

Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.

Soil and Water Loss Sensitivity Evaluation Based on Gis in Yudu County

Jing Zhao,Jianbo Tang,Zhi Li,Dekui Niu,Wenyuan Zhang,Xiaomin Guo 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6